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    METHODS AND COMPOSITIONS FOR NUCLEIC ACID ANALYSIS
    91.
    发明申请
    METHODS AND COMPOSITIONS FOR NUCLEIC ACID ANALYSIS 有权

    公开(公告)号：US20210002632A1

    公开(公告)日：2021-01-07

    申请号：US16935754

    申请日：2020-07-22

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Serge SAXONOV

    IPC: C12N15/10 ,  C12Q1/6876

    Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.

    System and method for isolating and analyzing cells
    92.
    发明授权
    System and method for isolating and analyzing cells 有权

    公开(公告)号：US10821440B2

    公开(公告)日：2020-11-03

    申请号：US16507905

    申请日：2019-07-10

    Applicant: Celsee Diagnostics, Inc.

    Inventor： Kalyan Handique ,  Vishal Sharma

    IPC: C12Q1/68 ,  B01L3/00 ,  C12M1/00 ,  G01N33/574 ,  C12Q1/6816 ,  G01N33/543 ,  G01N33/569 ,  G01N15/14 ,  C12Q1/6886 ,  G01N15/10

    Abstract: A system and method for isolating and analyzing single cells, wherein the system includes: an array of wells defined at a substrate, each well including an open surface and a well cavity configured to capture cells in one of a single-cell format and single-cluster format, and a fluid delivery module including a fluid reservoir superior to the array of wells through which fluid flow is controlled along a fluid path in a direction parallel to the broad face of the substrate; and wherein the method includes: capturing a population of non-cell particles into the array of wells in single-particle format; releasing, from the non-cell particles, a set of probes into the array of wells; capturing a population of cells into the array of wells in single-cell format; releasing biomolecules from each captured cell into the array of wells; and generating a set of genetic complexes comprising the biomolecules associated with a single captured cell and a subset of probes within individual wells of the array of wells.

    Digital immunoassay
    93.
    发明授权
    Digital immunoassay 有权

    公开(公告)号：US10809250B2

    公开(公告)日：2020-10-20

    申请号：US15752108

    申请日：2016-08-18

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Jan zur Megede ,  George Karlin-Neumann

    IPC: G01N33/542 ,  G01N33/58

    Abstract: Methods of detecting an analyte in a sample are provided. In one embodiment, the method comprises forming a partition comprising the sample, a binding agent capable of emitting a signal when bound to the analyte, and a marker capable of identifying the partition; allowing the binding agent to bind to the analyte, if present; and detecting the presence of the analyte in the sample by detecting the signal emitted from the binding agent in the partition.

    Methods and compositions for nucleic acid analysis
    96.
    发明授权
    Methods and compositions for nucleic acid analysis 有权

    公开(公告)号：US10760073B2

    公开(公告)日：2020-09-01

    申请号：US16223416

    申请日：2018-12-18

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Serge Saxonov

    IPC: C12N15/00 ,  C12Q1/68 ,  C12N15/10 ,  C12Q1/6876

    Abstract: Provided herein are methods, compositions, and kits for assays, many of which involve amplification reactions such as digital PCR or droplet digital PCR. The assays may be used for such applications as sequencing, copy number variation analysis, and others. In some cases, the assays involve subdividing a sample into multiple partitions (e.g., droplets) and merging the partitions with other partitions that comprise adaptors with barcodes.

    Value assignment for customizable quality controls
    100.
    发明授权
    Value assignment for customizable quality controls 有权

    公开(公告)号：US10697981B2

    公开(公告)日：2020-06-30

    申请号：US15849514

    申请日：2017-12-20

    Applicant: Bio-Rad Laboratories, Inc.

    Inventor： Alireza Ebrahim ,  Karl De Vore ,  Christopher Spates

    IPC: G01N33/96 ,  G01N33/78 ,  G01N33/48

    Abstract: Methods are provided for end users of diagnostic measurement procedures to prepare quality controls having desired analyte recoveries, estimate recoveries of quality controls already prepared, and compare estimated and measured recoveries. To prepare a quality control containing a particular analyte, a desired recovery of a measurement procedure for the analyte can be scaled by a correlation factor to obtain a target nominal concentration of the analyte in the quality control. Alternatively, the nominal concentration of an analyte in a quality control can be scaled by a correlation factor to obtain a predicted recovery of a measurement procedure for the analyte. The correlation factors can be based on recovery data previously obtained using the measurement procedure and optionally one or more reference procedures, and can be calculated using regression analysis of these data. Each quality control can be prepared by dissolving a number of solid beads containing the analyte(s) of interest in a volume of base matrix.

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