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1187 条记录 (耗时 0.035 秒)

    METHOD FOR ISOLATING SHORT-CHAIN NUCLEIC ACIDS
    94.
    发明申请
    METHOD FOR ISOLATING SHORT-CHAIN NUCLEIC ACIDS 有权
    用于分离短链核酸的方法

    公开(公告)号：US20110160446A1

    公开(公告)日：2011-06-30

    申请号：US12995341

    申请日：2009-05-12

    申请人： Christoph Ritt ,  Martin Horlitz ,  Markus Sprenger-Haussels

    发明人： Christoph Ritt ,  Martin Horlitz ,  Markus Sprenger-Haussels

    IPC分类号： C07H1/06

    CPC分类号： C07H1/08 ,  C12N15/1006

    摘要： The invention relates to a method and kits for isolating and/or purifying nucleic acids, in particular, short-chain nucleic acids, from a nucleic acid containing starting material, characterised by the following method steps: (a) bonding the nucleic acids to a nucleic acid bonding support material, wherein the starting material is brought into contact with the nucleic acid bonding support material in the presence of at least one chaotropic compound and preferably isopropanol, wherein the isopropanol is present in a concentration of ≧25% (v/v) and ≦35% (v/v), (b) optional elution of the bonded nucleic acids from the nucleic acid bonding support material. Said method is particularly suitable for the purification of foetal DNA from maternal blood.

    摘要翻译： 本发明涉及用于从含有起始物质的核酸分离和/或纯化核酸，特别是短链核酸的方法和试剂盒，其特征在于以下方法步骤：（a）将核酸结合至 核酸结合载体材料，其中原料在至少一种离液序列化合物和优选异丙醇的存在下与核酸键合载体材料接触，其中异丙醇以≥25％（v / v）的浓度存在 ）和＆lt; nlE; 35％（v / v），（b）从核酸粘合载体材料任选洗脱键合的核酸。 所述方法特别适用于从母体血液中纯化胎儿DNA。

    Method of Isolation of Nucleic Acids
    95.
    发明申请
    Method of Isolation of Nucleic Acids 有权
    核酸分离方法

    公开(公告)号：US20110124851A1

    公开(公告)日：2011-05-26

    申请号：US12624861

    申请日：2009-11-24

    申请人： Qi Guo

    发明人： Qi Guo

    IPC分类号： C07H21/04 ,  C07H21/00

    CPC分类号： C12N15/1006 ,  C12N15/1013

    摘要： A method of isolation of nucleic acids from a biological sample of cells comprising a combination of a solid phase cell nuclei isolation procedure with a solid phase nucleic acid isolation method.

    摘要翻译： 从细胞生物样品中分离核酸的方法，其包括固相细胞核分离程序与固相核酸分离方法的组合。

    METHODS AND DEVICES FOR PRODUCING BIOMOLECULES
    96.
    发明申请
    METHODS AND DEVICES FOR PRODUCING BIOMOLECULES 审中-公开
    用于生产生物分子的方法和装置

    公开(公告)号：US20110124101A1

    公开(公告)日：2011-05-26

    申请号：US12866127

    申请日：2009-02-06

    申请人： Jochen Urthaler ,  Christine Ascher ,  Daniel Bucheli

    发明人： Jochen Urthaler ,  Christine Ascher ,  Daniel Bucheli

    IPC分类号： C12N15/63 ,  C07H21/04 ,  B01D29/00

    CPC分类号： C12P19/34 ,  B03D1/028 ,  B03D1/082 ,  B03D1/1462 ,  B03D1/1468 ,  B03D1/1487 ,  B03D1/18 ,  B03D2203/003 ,  C12N15/1003 ,  C12N15/1006

    摘要： A scalable process and device for producing a bio molecule, in particular pharmaceutical grade plasmid DNA is described. The process includes the steps of alkaline lysis, neutralization and clarification and can be further extended. For separating the lysate and the precipitate an improved floatation method is disclosed. This method is based on attachment of CO2 bubbles on the precipitate floe. The CO2 is released from a carbonate salt during or after neutralization (acidification). The method of the invention is preferably carried out in an automated continuous mode applying devices for lysis and neutralization and a novel device for completely continuous clarification (separation of floes and clarified lysate).

    摘要翻译： 描述了用于生产生物分子，特别是药物级质粒DNA的可扩展的方法和装置。 该方法包括碱裂解，中和和澄清的步骤，并可进一步扩展。 为了分离裂解物和沉淀物，公开了改进的浮选方法。 这种方法是基于沉淀物上的二氧化碳气泡的附着。 在中和（酸化）期间或之后，CO 2从碳酸盐中释放出来。 本发明的方法优选以自动连续模式施加用于裂解和中和的装置和用于完全连续澄清（分离絮凝物和澄清裂解物）的新装置进行。

    METHOD FOR NUCLEIC ACIDS ISOLATION
    97.
    发明申请
    METHOD FOR NUCLEIC ACIDS ISOLATION 有权
    核酸分离方法

    公开(公告)号：US20110118457A1

    公开(公告)日：2011-05-19

    申请号：US12747802

    申请日：2008-12-12

    申请人： Jesus Ching

    发明人： Jesus Ching

    IPC分类号： C07H21/00 ,  B01D15/00

    CPC分类号： B01J20/3251 ,  B01D15/424 ,  B01J20/286 ,  C12N15/1006

    摘要： The present invention provides a method for isolating nucleic acids. The method comprises: contacting a sample containing nucleic acids with a solid phase in a first aqueous solution to provide a loaded solid phase; washing the loaded solid phase with a second aqueous solution to provide a washed solid phase; and eluting the washed solid phase with a low ionic strength liquid to obtain the isolated nucleic acids. The present invention also provides a kit for practicing the present method.

    摘要翻译： 本发明提供了分离核酸的方法。 该方法包括：使含有核酸的样品与固相在第一水溶液中接触以提供负载的固相; 用第二水溶液洗涤负载的固相以提供洗涤的固相; 并用低离子强度液体洗脱洗涤的固相以获得分离的核酸。 本发明还提供了用于实施本方法的试剂盒。

    Method of purifying RNA using kosmotropic salt
    99.
    发明授权
    Method of purifying RNA using kosmotropic salt 有权
    使用去离子水纯化RNA的方法

    公开(公告)号：US07923551B2

    公开(公告)日：2011-04-12

    申请号：US12110391

    申请日：2008-04-28

    申请人： Myo-yong Lee ,  Nam Huh ,  Joon-ho Kim

    发明人： Myo-yong Lee ,  Nam Huh ,  Joon-ho Kim

    IPC分类号： C07H21/00

    CPC分类号： C07H21/02 ,  C12N15/1003 ,  C12N15/1006

    摘要： The present invention provides a method of purifying RNA, including contacting a solid support with an acidic solution having a RNA-containing sample and a kosmotropic salt having a concentration of less than 1M, thereby binding the RNA to the solid support. According to the present invention, RNA is purified efficiently due to high RNA yield and low contamination by DNA. The present invention is particularly effective in purifying RNAs of 200 nucleotides or less.

    摘要翻译： 本发明提供了一种纯化RNA的方法，包括使固体支持物与具有含RNA样品的酸性溶液和浓度小于1M的感应性盐接触，从而将RNA与固体支持物结合。 根据本发明，由于高RNA产量和低DNA污染，RNA被有效地纯化。 本发明在纯化200个或更少核苷酸的RNA中特别有效。