摘要:
The present invention relates to methods and materials for the detection of ketone and aldehyde analytes in fluid samples by means of reacting analyte containing samples with a first solid matrix material to which a nitroprusside salt is coupled and a second solid matrix material to which an amine is covalently coupled. Methods and devices are also provided for ascertaining the fat catabolism effects of a weight loss dietary regimen comprising determining the breath acetone concentration of the subject.
摘要:
A diffraction immunoassay method in which a diffraction grating design of non-light disturbing primary binding reagent on an insoluble surface is conjugated with analyte in a sample. If the primary binding reagent-analyte conjugate is light disturbing, a diffraction grating is formed. If the primary binding reagent-analyte conjugate is non-light disturbing, the analyte is further conjugated with a secondary binding reagent which is labeled with a light disturbing material to form a diffraction grating. Light from a narrow band light source is then applied to the surface, and the intensity of beams of diffracted light formed by the diffraction grating is determined. The diffraction immunoassay plate for the method comprises a smooth insoluble surface having on the surface thereof, a diffraction grating design of lines of the primary binding reagent. The diffraction immunoassay apparatus comprises a light source, a platform for supporting the diffraction immunoassay plates in the path of a beam of light from the light source, and at least one light detector positioned to detect light diffracted by the diffraction grating.
摘要:
A composition, device and method for preparing a ketone control solution are disclosed. The composition comprises dimethylformamide and a Group I, II or III metal salt of a .beta.-keto acid ester. The salt has the structure ##STR1## in which R is lower alkyl of 1 to 6 carbon atoms, R' is an aliphatic or cyclic group having 1 to about 7 carbon atoms, M is a Group I, II or III metal ion and n is 1, 2 or 3. A carrier matrix incorporated with the composition can be affixed to a support member to form the device. Preferably, a hydrolyzing agent is included on the device in the same or another carrier matrix. The method for preparing the control solution comprises contacting a predetermined volume of an aqueous solution with the device for a predetermined time.
摘要:
A method of measuring the beta-hydroxybutyrate concentration of a urine sample including, in one step, the oxidation of beta-hydroxybutyrate to acetone and acetoacetate and the removal of pre-existing acetone/acetoacetate.
摘要:
In the process for the production of a diagnostic agent for the detection of ketones, consisting of an absorbent carrier impregnated in a first stage with an aqueous solution of an amino acid and tetrasodium ethylenediamine tetraacetate as buffer, dried and impregnated in a second stage with a solution of sodium nitroferricyanide in a solvent mixture one component of which is methanol and dried again, the improvement which comprises using as the second component of the solvent mixture, instead of dimethyl formamide, which is considered detrimental to health, quite a number of other solvents.
摘要:
A test strip for the detection of ketone bodies, comprising an absorbing material, a heavy metal salt and a nitroprusside salt. The test strip is useful in the diagnosis of various diseases such as diabetes, disorders in the digestive organs, renal insufficiency, uremia, self-poisoning or malignant carcinoma.
摘要:
Disclosed herein is a method for determining a concentration of an analyte in a fluid and a system that uses the method. The method comprises exposing a sorbent material having a hydroxylamine salt and a halochromic indicator sorbed thereon and/or therein to the fluid, and determining the concentration of analyte using the change of colour, or rate of change of colour of the sorbent material.
摘要:
A portable system is provided for measuring an analyte, such as acetone, in the breath or other bodily fluid of a user. The system includes a portable measurement device that analyzes fluid samples and generates corresponding measurements. The portable measurement device communicates with an application which runs on a smartphone or other mobile device of the user. The application tracks, and generates graphs of, the measurements, and may include various features for facilitating the analysis of the measurements.