公开(公告)号：US11906402B2

公开(公告)日：2024-02-20

申请号：US15356331

申请日：2016-11-18

Applicant: EMD Millipore Corporation

Inventor： David D. Newbold ,  Douglas L. Millard ,  Erwin Y. Yu ,  Paul T. Jeffers ,  Jeffrey W. Weber

IPC: G01N1/20 ,  G01N1/14 ,  C12M1/26 ,  G01N1/10 ,  C12M1/12

CPC classification number: G01N1/2035 ,  C12M33/04 ,  C12M37/00 ,  G01N1/10 ,  G01N1/14 ,  G01N1/20 ,  G01N2001/1445 ,  G01N2001/205

Abstract: A sample can be collected from an enclosed container by opening a sample collection valve and drawing the sample from the enclosed container. After delivery of the sample out of a fluid flow path, a sanitizing fluid can be directed along the fluid flow path to sanitize the fluid flow path.

公开(公告)号：US11691107B2

公开(公告)日：2023-07-04

申请号：US16762688

申请日：2018-11-01

Applicant: EMD Millipore Corporation

Inventor： Elizabeth M. Goodrich ,  Akshat Gupta ,  Herbert Lutz

IPC: B01D63/00 ,  B01D61/18 ,  B01D61/14 ,  B01D61/22 ,  B01D65/02

CPC classification number: B01D61/146 ,  B01D61/18 ,  B01D61/22 ,  B01D65/02 ,  B01D2311/04 ,  B01D2311/08 ,  B01D2311/25 ,  B01D2313/18 ,  B01D2313/243 ,  B01D2313/50 ,  B01D2315/10 ,  B01D2315/14 ,  B01D2315/16 ,  B01D2317/022 ,  B01D2317/04 ,  B01D2321/16

Abstract: Processes and systems for filtering a liquid sample are provided. Batches of a liquid sample can be routed to two or more cycling tanks (e.g., first and second cycling tanks). Upon filling a first cycling tank, a first batch of the liquid sample can be routed to a filtration assembly by a continuous diafiltration process that includes routing produced retentate back to the first cycling tank or to a collection vessel. Upon filling a second cycling tank, a second batch of the liquid sample is routed to the filtration assembly by a continuous diafiltration process that includes routing produced retentate back to the second cycling tank or to the collection vessel. The filling and continuous diafiltration of batches of the liquid sample continues to alternate between the two or more cycling tanks until a total product volume is processed.

公开(公告)号：US11634457B2

公开(公告)日：2023-04-25

申请号：US16358845

申请日：2019-03-20

Applicant: EMD Millipore Corporation

Inventor： Nanying Bian ,  Christopher Gillespie ,  Matthew T. Stone ,  Mikhail Kozlov ,  Jie Chen ,  Martin Siwak

IPC: B01D15/36 ,  C07K1/36 ,  C07K1/16 ,  B01D15/18 ,  C07K1/18 ,  C07K1/22 ,  C07K16/00 ,  B01J20/282 ,  C07K1/20 ,  B01D15/12 ,  B01J39/26 ,  B01J41/20 ,  B01J43/00 ,  B01J47/04 ,  B01J20/20 ,  B01D15/38

Abstract: The present invention provides novel and improved protein purification processes which incorporate certain types of carbonaceous materials and result in effective and selective removal of certain undesirable impurities without adversely affecting the yield of the desired protein product.

公开(公告)号：US11617988B2

公开(公告)日：2023-04-04

申请号：US16207874

申请日：2018-12-03

Applicant: EMD Millipore Corporation

Inventor： Herbert Lutz ,  Joseph Parrella ,  Bala Raghunath

IPC: B01D61/14 ,  B01D61/18 ,  B01D63/08 ,  B01D65/02 ,  C07K1/34

Abstract: The present invention provides, in various embodiments, hybrid single-pass tangential flow filtration assemblies, disposable single-pass tangential flow filtration assemblies, scalable single-pass tangential flow filtration assemblies and adaptable modular single-pass tangential flow filtration assemblies. In other embodiments, the invention relates to processes for recovering proteins from the surface of a filtration membrane in a single-pass tangential flow filtration assembly and for cleaning a tangential flow filtration assembly. In additional embodiments, the invention provides methods of increasing the processing capacity of a single-pass tangential flow filtration assembly.

公开(公告)号：US11602709B2

公开(公告)日：2023-03-14

申请号：US16328640

申请日：2017-08-29

Applicant: EMD Millipore Corporation

Inventor： Joseph Geringer ,  Robert Castro ,  Martin Szyk ,  Sophie Daudenarde ,  Joseph Almasian

IPC: B01D46/42 ,  B01D46/00

Abstract: Port protection device particularly suited for an encapsulated filter unit. In certain embodiments, the protection device comprises a main body defining an internal region configured to receive a port or the like protruding from an encapsulated filter. In certain embodiments, the main body includes a slot that allows for easy removal of the protection device from the port or the like. A tube management system may be provided to receive and hold tubing. One suitable tube management system includes two spaced tangs that extend from the main body of the protection device and are configured to cooperatively receive and hold tubing between them.

公开(公告)号：US20220402968A1

公开(公告)日：2022-12-22

申请号：US17756096

申请日：2020-12-11

Applicant: EMD Millipore Corporation

Inventor： Herbert Lutz ,  Kenneth Scott ,  Michael Bruce ,  Kristina Cunningham

IPC: C07K1/36 ,  C12M1/00 ,  C12N7/00 ,  C07K1/22 ,  C07K1/34 ,  B01D15/38 ,  B01D15/18 ,  B01D15/36 ,  B01D15/32

Abstract: Method and system for purifying a sample comprising a biomolecule of interest and impurities, comprising expressing said biomolecule of interest in a bioreactor to form a product sample comprising said biomolecule of interest and impurities; subjecting said product sample to filtration to form a clarified product sample; subjecting said clarified product sample to affinity chromatography to remove impurities; subsequently subjecting said product sample to diafiltration followed by virus filtration and optional concentration. The buffer used during the diafiltration step (and thus in the virus filtration step) is the buffer desired for the final formulation of the product.

公开(公告)号：USD970517S1

公开(公告)日：2022-11-22

申请号：US29758166

申请日：2020-11-12

Applicant: Sigma-Aldrich Co. LLC ,  EMD Millipore Corporation

Designer： Sarah Trice ,  Lindsey Rickershauser ,  Xiang Wu ,  Churl Oh ,  Nadia Brugger ,  Emma Gardener

公开(公告)号：USD962282S1

公开(公告)日：2022-08-30

申请号：US29758161

申请日：2020-11-12

Applicant: Sigma-Aldrich Co. LLC ,  EMD Millipore Corporation

Designer： Sarah Trice ,  Lindsey Rickershauser ,  Xiang Wu ,  Churl Oh ,  Nadia Brugger ,  Emma Gardener

公开(公告)号：US20220186259A1

公开(公告)日：2022-06-16

申请号：US17605190

申请日：2020-06-25

Applicant: EMD Millipore Corporation

Inventor： Naohisa Yoshioka

IPC: C12N15/86 ,  C12N9/12

Abstract: Synthetic, self-replicating RNA vectors comprising sequence encoding at least one immortalization protein, and use of the self-replicating RNA vectors to extend the lifespan of primary cell populations and expand said populations of primary cells.

公开(公告)号：US20220186235A1

公开(公告)日：2022-06-16

申请号：US17606644

申请日：2020-05-13

Applicant: EMD Millipore Corporation

Inventor： Naohisa Yoshioka

IPC: C12N15/79 ,  C12N15/10 ,  C12N9/22 ,  C12N15/11

Abstract: Synthetic, noninfectious, self-replicating RNA vectors that encode CRISPR proteins are provided. Each self-replicating RNA vector comprises a sequence encoding a plurality of non-structural replication complex proteins from an alphavirus and a sequence encoding a CRISPR protein. Also provided are methods for genome editing in which a synthetic self-replicating RNA vector is transfected into cells along with at least one corresponding guide RNA.