利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

153 条记录 (耗时 0.027 秒)

    NUCLEIC ACID SEQUENCING BY NANOPORE DETECTION OF TAG MOLECULES
    111.
    发明申请
    NUCLEIC ACID SEQUENCING BY NANOPORE DETECTION OF TAG MOLECULES 审中-公开
    通过纳米检测标签分子的核酸序列

    公开(公告)号：US20150119259A1

    公开(公告)日：2015-04-30

    申请号：US14391337

    申请日：2013-04-08

    申请人： Jingyue JU ,  Randall DAVIS ,  Roger CHEN ,  THE TRUSTEES OF COLUMBIA UNIVERSITY IN THE CITY OF NEW YORK ,  GENIA TECHNOLOGIES, INC.

    发明人： Jingyue Ju ,  Randall Davis ,  Roger Chen

    IPC分类号： C12Q1/68

    摘要： This disclosure provides systems and methods for sequencing nucleic acids using nucleotide analogues and translocation of tags from incorporated nucleotide analogues through a nanopore. In aspects, this disclosure is related to composition, method, and system for sequencing a nucleic acid using tag molecules and detection of translocation through a nanopore of tags released from incorporation of the molecule.

    摘要翻译： 本公开提供了使用核苷酸类似物对核酸进行测序的系统和方法，以及通过纳米孔从掺入的核苷酸类似物转移标签。 在一些方面，本公开涉及使用标签分子对核酸进行测序的组合物，方法和系统，以及通过从分子掺入释放的标记的纳米孔的易位检测。

    Noise shielding techniques for ultra low current measurements in biochemical applications
    112.
    发明授权
    Noise shielding techniques for ultra low current measurements in biochemical applications 有权
    用于生化应用中超低电流测量的噪声屏蔽技术

    公开(公告)号：US08928097B2

    公开(公告)日：2015-01-06

    申请号：US13972616

    申请日：2013-08-21

    申请人： Genia Technologies, Inc.

    发明人： Roger J. A. Chen

    IPC分类号： H01L23/552 ,  H01L49/02

    CPC分类号： H01L23/552 ,  B82Y15/00 ,  G01N27/44756 ,  G01N27/44791 ,  G01N33/48721 ,  H01L21/76877 ,  H01L23/5225 ,  H01L23/585 ,  H01L28/60 ,  H01L2924/0002 ,  H01L2924/00

    摘要： A device having an integrated noise shield is disclosed. The device includes a plurality of vertical shielding structures substantially surrounding a semiconductor device. The device further includes an opening above the semiconductor device substantially filled with a conductive fluid, wherein the plurality of vertical shielding structures and the conductive fluid shield the semiconductor device from ambient radiation. In some embodiments, the device further includes a conductive bottom shield below the semiconductor device shielding the semiconductor device from ambient radiation. In some embodiments, the opening is configured to allow a biological sample to be introduced into the semiconductor device. In some embodiments, the vertical shielding structures comprise a plurality of vias, wherein each of the plurality of vias connects more than one conductive layers together. In some embodiments, the device comprises a nanopore device, and wherein the nanopore device comprises a single cell of a nanopore array.

    摘要翻译： 公开了一种具有集成噪声屏蔽的装置。 该装置包括基本上围绕半导体器件的多个垂直屏蔽结构。 该器件还包括在基本上填充有导电流体的半导体器件上方的开口，其中多个垂直屏蔽结构和导电流体屏蔽半导体器件免受环境辐射。 在一些实施例中，该器件还包括在半导体器件下面的导电底部屏蔽，该半导体器件屏蔽半导体器件免受环境辐射。 在一些实施例中，开口被配置为允许生物样品被引入到半导体器件中。 在一些实施例中，垂直屏蔽结构包括多个通孔，其中多个通孔中的每一个将不止一个导电层连接在一起。 在一些实施方案中，所述装置包括纳米孔装置，并且其中所述纳米孔装置包括纳米孔阵列的单个细胞。

    NANOPORE ARRAYS
    113.
    发明申请
    NANOPORE ARRAYS 有权

    公开(公告)号：US20140221249A1

    公开(公告)日：2014-08-07

    申请号：US13759701

    申请日：2013-02-05

    申请人： Genia Technologies, Inc.

    发明人： Roger J.A. Chen ,  David J. Fullager

    IPC分类号： G01N27/327

    CPC分类号： G01N33/48721

    摘要： A method of analyzing molecules using a nanopore array including a plurality of cells included on a chip is disclosed. Nanopores are caused to be formed in at least a portion of the plurality of the cells. A first physical measurement of the nanopores is evaluated. It is determined whether to cause the molecules to interact with the nanopores. At least a portion of the nanopores is caused to interact with the molecules. A second physical measurement of the nanopores that indicates a property of the molecules is evaluated. It is determined whether to cause the nanopores to be reformed so that the cells may be reused to interact with additional molecules.

    摘要翻译： 公开了一种使用包括在芯片上的多个单元的纳米孔阵列来分析分子的方法。 导致在多个电池的至少一部分中形成纳米孔。 评估纳米孔的第一物理测量。 确定是否使分子与纳米孔相互作用。 导致至少一部分纳米孔与分子相互作用。 评估表明分子性质的纳米孔的第二物理测量。 确定是否使纳米孔被重整，使得细胞可以重新用于与另外的分子相互作用。

    Nanopore Based Molecular Detection and Sequencing
    114.
    发明申请
    Nanopore Based Molecular Detection and Sequencing 审中-公开
    基于纳米孔的分子检测和测序

    公开(公告)号：US20130244340A1

    公开(公告)日：2013-09-19

    申请号：US13745688

    申请日：2013-01-18

    申请人： GENIA TECHNOLOGIES, INC.

    发明人： Randall Davis ,  Roger Chen

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6869 ,  C12Q1/6874 ,  G01N33/48721 ,  C12Q2521/101 ,  C12Q2565/607

    摘要： This disclosure provides systems and methods for molecular identification and polymer (e.g., nucleic acid) sequencing using nanopores. The polymer may be passed through or in proximity to the nanopore and various subunits of the polymer may affect the current flowing through the nanopore. The various subunits may be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. In some cases, the polymerization of tagged nucleotides presents tag molecules to the nanopore that can be identified by measuring the current at a plurality of voltages applied across the nanopore and/or membrane. Also provided herein are systems and methods for sequencing both the sense and anti-sense strand of a double stranded nucleic acid molecule with a nanopore and methods for using ribonucleic acid (RNA) speed bump molecules to slow the passage of a nucleic acid molecule through or in proximity to a nanopore.

    摘要翻译： 本公开提供用于分子鉴定和使用纳米孔的聚合物（例如，核酸）测序的系统和方法。 聚合物可以通过纳米孔或靠近纳米孔，聚合物的各种亚基可能影响流过纳米孔的电流。 可以通过测量跨过纳米孔和/或膜施加的多个电压的电流来识别各种亚基。 在一些情况下，标记的核苷酸的聚合会向纳米孔呈现标签分子，其可以通过测量施加在纳米孔和/或膜上的多个电压的电流来鉴定。 本文还提供了用纳米孔对双链核酸分子的有义链和反义链进行测序的系统和方法，以及使用核糖核酸（RNA）速度突变分子来减缓核酸分子通过的方法或 靠近纳米孔。

    High speed molecular sensing with nanopores
    116.
    发明授权
    High speed molecular sensing with nanopores 有权

    公开(公告)号：US10421995B2

    公开(公告)日：2019-09-24

    申请号：US14521469

    申请日：2014-10-23

    申请人： Genia Technologies, Inc.

    发明人： Randall Davis

    IPC分类号： C12Q1/68 ,  C12Q1/00 ,  C12M1/34 ,  C12Q1/6869 ,  C12Q1/6816

    摘要： Described herein are methods and devices for capturing and determining the identity of molecules using nanopores. The molecules can be counted, sorted and/or binned rapidly in a parallel manner using a large number of nanopores (e.g., 132,000 nanopores reading 180 million molecules in 1 hour). This fast capture and reading of a molecule can be used to capture probe molecules or other molecules that have been generated to represent an original, hard to detect molecule or portions of an original molecule. Precise counting of sample molecules or surrogates for sample molecules can occur. The methods and devices described herein can, among other things, replace flow cytometers and other counting instruments (e.g., while providing increased precision and throughput relative to a flow cytometer). In some cases, the devices and methods capture and hold particular molecules or surrogates of molecules in the nanopores and then eject them into clean solution to perform a capture, sorting, and binning function similar to flow cytometers.

    Non-faradaic, capacitively coupled measurement in a nanopore cell array
    119.
    发明授权
    Non-faradaic, capacitively coupled measurement in a nanopore cell array 有权

    公开(公告)号：US10393700B2

    公开(公告)日：2019-08-27

    申请号：US16157019

    申请日：2018-10-10

    申请人： Genia Technologies, Inc.

    发明人： Roger J. A. Chen

    IPC分类号： G01N27/327 ,  G01N27/447 ,  G01N33/487

    摘要： A method of identifying a molecule is disclosed. A molecule is drawn to a nanopore by applying a first voltage signal to a pair of electrodes during a first period, wherein the first voltage signal causes a first ionic current through the nanopore that is indicative of a property of a portion of the molecule proximate to the nanopore. The molecule is released from the nanopore by applying a second voltage signal to the pair of electrodes during a second period, wherein the second voltage signal causes a second ionic current through the nanopore. The first period and the second period are determined based at least in part on a net ionic current through the nanopore comprising the first ionic current and the second ionic current.