公开(公告)号：US06680171B1

公开(公告)日：2004-01-20

申请号：US09489155

申请日：2000-01-21

申请人： Jian Ni ,  Craig A. Rosen ,  Vishva M. Dixit ,  Reiner L. Gentz ,  Joseph J. Kenny

发明人： Jian Ni ,  Craig A. Rosen ,  Vishva M. Dixit ,  Reiner L. Gentz ,  Joseph J. Kenny

IPC分类号： C07H2104

CPC分类号： C12N9/6475 ,  A61K38/00

摘要： The present invention relates to a novel I-FLICE-1 or I-FLICE-2 protein which is a novel inhibitor of INFR-1 and CD-95 induced apoptosis. In particular, isolated nucleic acid molecules are provided encoding the human I-FLICE-1 or I-FLICE-2 protein. I-FLICE-1 or I-FLICE-2 polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of I-FLICE-1 or I-FLICE-2 activity. Also provided are therapeutic methods for treating diseases and disorders associated with apoptosis.

摘要翻译： 本发明涉及一种新型的I-FLICE-1或I-FLICE-2蛋白，其是INFR-1和CD-95诱导的凋亡的新型抑制剂。 特别地，提供编码人I-FLICE-1或I-FLICE-2蛋白的分离的核酸分子。 还提供I-FLICE-1或I-FLICE-2多肽，载体，宿主细胞和用于制备它的重组方法。 本发明还涉及用于鉴定I-FLICE-1或I-FLICE-2活性的激动剂和拮抗剂的筛选方法。 还提供了用于治疗与凋亡相关的疾病和病症的治疗方法。

公开(公告)号：US06623938B2

公开(公告)日：2003-09-23

申请号：US09009893

申请日：1998-01-21

申请人： Jian Ni ,  Craig A. Rosen ,  Vishva M. Dixit ,  Reiner L. Gentz ,  Joseph J. Kenny

发明人： Jian Ni ,  Craig A. Rosen ,  Vishva M. Dixit ,  Reiner L. Gentz ,  Joseph J. Kenny

IPC分类号： C12P2106

CPC分类号： C12N9/6475 ,  A61K38/00

摘要： The present invention relates to a novel I-FLICE-1 or I-FLICE-2 protein which is a novel inhibitor of TNFR-1 and CD-95 induced apoptosis. In particular, isolated nucleic acid molecules are provided encoding the human I-FLICE-1 or I-FLICE-2 protein. I-FLICE-1 or I-FLICE-2 polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of I-FLICE-1 or I-FLICE-2 activity. Also provided are therapeutic methods for treating diseases and disorders associated with apoptosis.

摘要翻译： 本发明涉及一种新型的I-FLICE-1或I-FLICE-2蛋白，其是TNFR-1和CD-95诱导的细胞凋亡的新型抑制剂。 特别地，提供编码人I-FLICE-1或I-FLICE-2蛋白的分离的核酸分子。 还提供I-FLICE-1或I-FLICE-2多肽，载体，宿主细胞和用于制备它的重组方法。 本发明还涉及用于鉴定I-FLICE-1或I-FLICE-2活性的激动剂和拮抗剂的筛选方法。 还提供了用于治疗与凋亡相关的疾病和病症的治疗方法。

公开(公告)号：US06617132B2

公开(公告)日：2003-09-09

申请号：US09940497

申请日：2001-08-29

申请人： Jian Ni ,  Reiner L. Gentz ,  Guo-Liang Yu ,  Craig A. Rosen

发明人： Jian Ni ,  Reiner L. Gentz ,  Guo-Liang Yu ,  Craig A. Rosen

IPC分类号： C12N510

CPC分类号： C07K14/8139 ,  A61K38/00

摘要： Disclosed is a human CysE polypeptide and DNA (RNA) encoding such polypeptide. Also provided is a procedure for producing such polypeptide by recombinant techniques. Also disclosed are methods for utilizing such polypeptide for treating osteoporosis, tumor metastases, microbial infections, viral infection, septic shock, inflammation, retinal irritation, caries, cachicia and muscle wasting. Diagnostic methods for detecting mutations in the coding sequence and alterations in the concentration of the polypeptides in a sample derived from a host are also disclosed.

摘要翻译： 公开了编码这种多肽的人CysE多肽和DNA（RNA）。 还提供了通过重组技术产生这种多肽的方法。 还公开了利用这种多肽治疗骨质疏松症，肿瘤转移，微生物感染，病毒感染，败血性休克，炎症，视网膜刺激，龋齿，眼球和肌肉消瘦的方法。 还公开了用于检测编码序列中的突变和衍生自宿主的样品中多肽浓度变化的诊断方法。

公开(公告)号：US06548262B2

公开(公告)日：2003-04-15

申请号：US09827948

申请日：2001-04-06

申请人： Reiner L. Gentz ,  Tsu-An Hsu ,  Craig A. Rosen ,  Jian Ni

发明人： Reiner L. Gentz ,  Tsu-An Hsu ,  Craig A. Rosen ,  Jian Ni

IPC分类号： G01N3350

CPC分类号： C07K14/8114 ,  A61K38/00 ,  C07K2319/00

摘要： The present invention relates to a novel TFPI-3 protein which is a member of the tissue factor protease inhibitor family. In particular, isolated nucleic acid molecules are provided encoding human TFPI-3 proteins. TFPI-3 polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of TFPI-3 activity. Also provided are diagnostic methods for detecting hemostasis system-related disorders and therapeutic methods for treating hemostatis system-related disorders.

摘要翻译： 本发明涉及作为组织因子蛋白酶抑制剂家族成员的新型TFPI-3蛋白。 特别地，提供编码人TFPI-3蛋白质的分离的核酸分子。 还提供TFPI-3多肽，载体，宿主细胞和用于制备它们的重组方法。 本发明还涉及用于鉴定TFPI-3活性的激动剂和拮抗剂的筛选方法。 还提供了用于检测止血系统相关疾病的诊断方法和治疗止血系统相关疾病的治疗方法。

公开(公告)号：US06521742B2

公开(公告)日：2003-02-18

申请号：US09345790

申请日：1999-07-01

申请人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen

发明人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen

IPC分类号： C07K1452

CPC分类号： C07K14/5255

摘要： The present invention concerns a novel member of the tumor necrosis factor (TNF) family of cytokines. In particular, isolated nucleic acid molecules are provided encoding the endokine alpha protein. Endokine alpha polypeptides are also provided, as are vectors, host cells and recombinant methods for producing the same. Also provided are diagnostic and therapeutic methods concerning TNF family-related disorders.

摘要翻译： 本发明涉及细胞因子的肿瘤坏死因子（TNF）家族的新成员。 特别地，提供了分离的核酸分子，其编码内丝蛋白α蛋白。 还提供了内含子α多肽，载体，宿主细胞以及用于制备它们的重组方法也是如此。 还提供了关于TNF家族相关疾病的诊断和治疗方法。

公开(公告)号：US06403770B1

公开(公告)日：2002-06-11

申请号：US09589287

申请日：2000-06-08

申请人： Guo-Liang Yu ,  Reinhard Ebner ,  Jian Ni ,  Craig A. Rosen

发明人： Guo-Liang Yu ,  Reinhard Ebner ,  Jian Ni ,  Craig A. Rosen

IPC分类号： C07K1600

CPC分类号： C07K16/24 ,  A61K38/00 ,  A61K48/00 ,  A61K2039/505 ,  C07H21/04 ,  C07K14/52 ,  C07K14/715 ,  C07K16/2875 ,  C07K2317/34 ,  C07K2317/622 ,  C07K2317/76 ,  G01N33/564 ,  G01N33/6863 ,  G01N2333/52 ,  G01N2500/00 ,  G01N2500/02 ,  G01N2800/245 ,  Y02A50/473

摘要： The present invention relates to a novel Neutrokine-alpha, and a splice variant thereof designated Neutrokine-alphaSV, polynucleotides and polypeptides which are members of the TNF family. In particular, isolated nucleic acid molecules are provided encoding the human Neutrokine-alpha and/or Neutrokine-alphaSV polypeptides, including soluble forms of the extracellular domain. Neutrokine-alpha and/or Neutrokine-alphaSV polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of Neutrokine-alpha and/or Neutrokine-alphaSV activity. Also provided are diagnostic methods for detecting immune system-related disorders and therapeutic methods for treating immune system-related disorders.

摘要翻译： 本发明涉及一种新的Neutrokine-α及其剪接变体，其命名为Neutrokine-αSV，作为TNF家族成员的多核苷酸和多肽。 特别地，提供编码人Neutrokine-α和/或中枢神经素-αSV多肽的分离的核酸分子，包括细胞外结构域的可溶形式。 还提供了中和型α和/或中枢神经素αSV多肽，载体，宿主细胞和用于制备它们的重组方法。 本发明进一步涉及用于鉴定中和性α和/或中枢神经素αSV活性的激动剂和拮抗剂的筛选方法。 还提供了用于检测免疫系统相关疾病的诊断方法和用于治疗免疫系统相关疾病的治疗方法。

公开(公告)号：US06342363B1

公开(公告)日：2002-01-29

申请号：US09013895

申请日：1998-01-27

申请人： Jian Ni ,  Craig A. Rosen ,  James G. Pan ,  Reiner L. Gentz ,  Vishva M. Dixit

发明人： Jian Ni ,  Craig A. Rosen ,  James G. Pan ,  Reiner L. Gentz ,  Vishva M. Dixit

IPC分类号： G01N3353

CPC分类号： C07K14/70578

摘要： The present invention relates to novel Death Domain Containing Receptor-4 (DR4) proteins which are members of the tumor necrosis factor (TNF) receptor family. In particular, isolated nucleic acid molecules are provided encoding the human DR4 proteins. DR4 polypeptides are also provided as are vectors, host cells and recombinant methods for producing the same. The invention further relates to screening methods for identifying agonists and antagonists of DR4 activity.

摘要翻译： 本发明涉及作为肿瘤坏死因子（TNF）受体家族成员的新的含有死亡域的受体-4（DR4）蛋白。 特别地，提供编码人DR4蛋白的分离的核酸分子。 还提供了DR4多肽，载体，宿主细胞和用于制备它们的重组方法。 本发明还涉及用于鉴定DR4活性的激动剂和拮抗剂的筛选方法。

公开(公告)号：US5998171A

公开(公告)日：1999-12-07

申请号：US912227

申请日：1997-08-15

申请人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen

发明人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen

IPC分类号： C07K14/525 ,  C12N15/28 ,  C12N15/00

CPC分类号： C07K14/5255

摘要： The present invention novel member of the tumor necrosis factor (TNF) family of cytokines. In particular, isolated nucleic acid molecules are provided encoding the endokine alpha protein. Endokine alpha polypeptides are also provided, as are vectors, host cells and recombinant methods for producing the same. Also provided are diagnostic and therapeutic methods concerning TNF family-related disorders.

摘要翻译： 本发明细胞因子的肿瘤坏死因子（TNF）家族的新成员。 特别地，提供了分离的核酸分子，其编码内丝蛋白α蛋白。 还提供了内含子α多肽，载体，宿主细胞以及用于制备它们的重组方法也是如此。 还提供了关于TNF家族相关疾病的诊断和治疗方法。

公开(公告)号：US07820798B2

公开(公告)日：2010-10-26

申请号：US11837082

申请日：2007-08-10

申请人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen ,  Jun Zhang

发明人： Guo-Liang Yu ,  Jian Ni ,  Craig A. Rosen ,  Jun Zhang

IPC分类号： C07K16/28 ,  C07K16/18 ,  C07K16/22 ,  C07K16/46 ,  C12N5/00 ,  G01N33/53

CPC分类号： A61K51/08 ,  C07K16/241 ,  Y10S930/144

摘要： Human TNF-gamma-alpha and TNF-gamma-beta polypeptides and DNA (RNA) encoding such polypeptides and a procedure for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for utilizing such polypeptides to inhibit cellular growth, for example in a tumor or cancer, for facilitating wound-healing, to provide resistance against infection, induce inflammatory activities, and stimulating the growth of certain cell types to treat diseases, for example restenosis. Also disclosed are diagnostic methods for detecting a mutation in the TNF-gamma-alpha and TNF-gamma-beta nucleic acid sequences or overexpression of the TNF-gamma-alpha and/or TNF-gamma-beta polypeptides. Antagonists against such polypeptides and their use as a therapeutic to treat cachexia, septic shock, cerebral malaria, inflammation, arthritis and graft-rejection are also disclosed.

摘要翻译： 公开了人类TNF-γ-α和TNF-γ-β多肽和编码这种多肽的DNA（RNA）以及通过重组技术产生此类多肽的方法。 还公开了利用这些多肽抑制细胞生长的方法，例如在肿瘤或癌症中，用于促进伤口愈合，提供抗感染，诱导炎症活性和刺激某些细胞类型的生长以治疗疾病， 示例再狭窄。 还公开了用于检测TNF-γ-α和TNF-γ-β核酸序列中的突变或TNF-γ-α和/或TNF-γ-β多肽的过表达的诊断方法。 还公开了抗这些多肽的拮抗剂及其作为治疗性恶病质，败血性休克，脑疟疾，炎症，关节炎和移植排斥的治疗剂的用途。

公开(公告)号：US20090305991A1

公开(公告)日：2009-12-10

申请号：US12538668

申请日：2009-08-10

申请人： Daniel R. Soppet ,  Paul A. Moore ,  Yanggu Shi ,  Steven M. Ruben ,  Craig A. Rosen ,  David W. LaFleur ,  Henrik Olsen ,  Reinhard Ebner ,  Kimberly Florence ,  Paul Young ,  George Komatsoulis ,  Jian Ni

发明人： Daniel R. Soppet ,  Paul A. Moore ,  Yanggu Shi ,  Steven M. Ruben ,  Craig A. Rosen ,  David W. LaFleur ,  Henrik Olsen ,  Reinhard Ebner ,  Kimberly Florence ,  Paul Young ,  George Komatsoulis ,  Jian Ni

IPC分类号： A61K38/16 ,  C12N15/11 ,  C12N15/00 ,  C12N15/87 ,  C12N5/06 ,  C12N1/21 ,  C07K14/435 ,  C07K16/18 ,  C12P21/02 ,  C12Q1/68 ,  C12Q1/02 ,  G01N33/566

CPC分类号： C07K14/47 ,  A61K38/00

摘要： The present invention relates to 33 novel human secreted proteins and isolated nucleic acids containing the coding regions of the genes encoding such proteins. Also provided are vectors, host cells, antibodies, and recombinant methods for producing human secreted proteins. The invention further relates to diagnostic and therapeutic methods useful for diagnosing and treating diseases, disorders, and/or conditions related to these novel human secreted proteins.

摘要翻译： 本发明涉及33种新型人分泌蛋白和含有编码这种蛋白质的基因的编码区的分离的核酸。 还提供了用于产生人分泌蛋白质的载体，宿主细胞，抗体和重组方法。 本发明还涉及用于诊断和治疗与这些新型人分泌蛋白相关的疾病，病症和/或病症的诊断和治疗方法。