公开(公告)号：US20210171459A1

公开(公告)日：2021-06-10

申请号：US16932405

申请日：2020-07-17

Applicant: Genomatica, Inc.

Inventor： Rudolf Philippus Maria Guit ,  Thomas Van Der Does ,  Lourina Madeleine Raamsdonk

IPC: C07D201/08 ,  C07D223/10 ,  C07D201/16 ,  C08G69/16

Abstract: The invention relates to a method for preparing caprolactam comprising recovering a mixture containing 6-aminocaproic acid, from a culture medium comprising biomass, and thereafter cyclising the 6-aminocaproic acid in the presence of superheated steam, thereby forming caprolactam, wherein the weight to weight ratio carbohydrate to 6-aminocaproic acid in said mixture is 0.03 or less.

公开(公告)号：US11008597B2

公开(公告)日：2021-05-18

申请号：US15301754

申请日：2015-04-10

Applicant: Genomatica, Inc.

Inventor： Stephen B. Del Cardayre ,  Andreas W. Schirmer ,  Myong Ko ,  Haibo Wang

IPC: C12P17/08 ,  C12P17/04 ,  C12P7/44 ,  C12P7/42

Abstract: The present disclosure relates to processes that combine microbial production of organic intermediates and subsequent synthetic transformation to provide compounds of industrial value, including compounds used in fragrances.

公开(公告)号：US20210101855A1

公开(公告)日：2021-04-08

申请号：US16498313

申请日：2018-03-29

Applicant: Genomatica, Inc.

Inventor： Julia Khandurina ,  Rachel Diane Pacheco ,  Lauri Hannunpoika Suominen ,  Rainer Wagester

IPC: C07C29/80 ,  B01D3/14 ,  C07C29/94 ,  B01D15/36

Abstract: Provided herein are bioderived 1,3-butanediol compositions and systems and processes for producing such bioderived 1,3-butanediol compositions.

公开(公告)号：US10941454B2

公开(公告)日：2021-03-09

申请号：US15578488

申请日：2016-05-26

Applicant: GENOMATICA, INC. ,  BRASKEM S.A.

Inventor： Stephanie J. Culler ,  Robert J. Haselbeck ,  Harish Nagarajan ,  Iuri Estrada Gouvea ,  Daniel Johannes Koch ,  Mateus Schreiner Garcez Lopes ,  Lucas Pedersen Parizzi

IPC: C12N9/88 ,  C12N9/90 ,  C07K16/40 ,  C08F36/06 ,  C12N15/82 ,  C12P5/02 ,  C12P7/16

Abstract: In alternative embodiments, provided are non-natural or genetically engineered vinylisomerase-dehydratase enzymes, including alkenol dehydratases, linalool dehydratases and crotyl alcohol dehydratases. In alternative embodiments, provided are non-natural or genetically engineered polypeptides having an activity comprising, for example, a vinylisomerase-dehydratase, an alkenol dehydratase, a linalool dehydratase and/or a crotyl alcohol dehydratase activity, or a combination thereof. In alternative embodiments, also provided are non-natural or genetically engineered nucleic acids (polynucleotides) encoding polypeptides described herein, expression or cloning vehicles comprising or having contained therein nucleic acids as described herein, and non-natural or genetically engineered cells comprising or having contained therein nucleic acids as described herein. In alternative embodiments, also provided are methods for making various organic compounds, including methyl vinyl carbinol and butadiene.

公开(公告)号：US10900057B2

公开(公告)日：2021-01-26

申请号：US15039052

申请日：2014-12-05

Applicant: Genomatica, Inc.

Inventor： Stephen B. Del Cardayre ,  Louis G. Hom

IPC: C12P13/00 ,  C12N9/16 ,  C12N9/02 ,  C12N9/10 ,  C12N9/00 ,  C12N9/06

Abstract: The disclosure relates to recombinant microorganisms for the production of fatty amines and derivatives thereof. Further contemplated are cultured recombinant host cells as well as methods of producing fatty amines by employing these host cells.

公开(公告)号：US20210002673A1

公开(公告)日：2021-01-07

申请号：US16888339

申请日：2020-05-29

Applicant: Genomatica, Inc.

Inventor： Stephanie J. Culler ,  Mark J. Burk ,  Robin E. Osterhout ,  Priti Pharkya

IPC: C12P7/04 ,  C12N15/52 ,  C12P5/02 ,  C12P7/40 ,  C12N9/90 ,  C12N9/88 ,  C12N9/04

Abstract: The invention provides a non-naturally occurring microbial organism having a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol, pathway. The microbial organism contains at least one exogenous nucleic acid encoding an enzyme in a pathway. The invention additionally provides a method for producing butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol. The method can include culturing a butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol-producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding a pathway enzyme in a sufficient amount, and under conditions and for a sufficient period of time to produce butadiene, crotyl alcohol, 2,4-pentadienoate, 3-buten-2-ol, or 3-buten-1-ol.

公开(公告)号：US20200370024A1

公开(公告)日：2020-11-26

申请号：US16661667

申请日：2019-10-23

Applicant: Genomatica, Inc.

Inventor： Baolong ZHU ,  Andreas W. Schirmer ,  Cindy CHANG

IPC: C12N9/02 ,  C12P7/64

Abstract: The disclosure relates to omega-hydroxylase-related fusion polypeptides that result in improved omega-hydroxylated fatty acid derivative production when expressed in recombinant host cells. The disclosure further relates to microorganisms for expressing the omega-hydroxylase-related fusion polypeptides for the production of omega-hydroxylated fatty acid derivatives.

公开(公告)号：US10570074B2

公开(公告)日：2020-02-25

申请号：US15705012

申请日：2017-09-14

Applicant: REG Life Sciences LLC

Inventor： Haibo Wang

IPC: C07C33/035

Abstract: The disclosure relates to the field of specialty chemicals. In particular, the disclosure provides novel 1,3-fatty-diol compounds and derivatives thereof which are useful e.g., in the production of personal care products, surfactants, detergents, polymers, paints, coatings, and as emulsifiers, emollients, and thickeners in cosmetics and foods, as industrial solvents and plasticizers, etc.

公开(公告)号：US10563180B2

公开(公告)日：2020-02-18

申请号：US15027169

申请日：2014-10-03

Applicant: Genomatica Inc.

Inventor： Stefan Andrae ,  Michael Patrick Kuchinskas ,  Jingyi Li ,  Harish Nagarajan ,  Priti Pharkya

IPC: C12N9/04 ,  C12P7/24

Abstract: Described herein are non-natural NAD+-dependent alcohol dehydrogenases (ADHs) capable of at least two fold greater conversion of methanol or ethanol to formaldehyde or acetaldehyde, respectively, as compared to its unmodified counterpart. Nucleic acids encoding the non-natural alcohol dehydrogenases, as well as expression constructs including the nucleic acids, and engineered cells comprising the nucleic acids or expression constructs are described. Also described are engineered cells expressing a non-natural NAD+-dependent alcohol dehydrogenase, optionally include one or more additional metabolic pathway transgene(s), methanol metabolic pathway genes, target product pathway genes, cell culture compositions including the cells, methods for promoting production of the target product or intermediate thereof from the cells, compositions including the target product or intermediate, and products made from the target product or intermediate.

公开(公告)号：US10550411B2

公开(公告)日：2020-02-04

申请号：US15889788

申请日：2018-02-06

Applicant: Genomatica, Inc.

Inventor： Mark J. Burk ,  Christophe H. Schilling ,  Anthony P. Burgard ,  John D. Trawick

IPC: C12P7/18 ,  C12N9/02 ,  C12N9/10 ,  C12N9/00 ,  C12P19/32 ,  C12N15/70

Abstract: The invention provides a non-naturally occurring microbial organism having an acetyl-CoA pathway and the capability of utilizing syngas or syngas and methanol. In one embodiment, the invention provides a non-naturally occurring microorganism, comprising one or more exogenous proteins conferring to the microorganism a pathway to convert CO, CO2 and/or H2 to acetyl-coenzyme A (acetyl-CoA), methyl tetrahydrofolate (methyl-THF) or other desired products, wherein the microorganism lacks the ability to convert CO or CO2 and H2 to acetyl-CoA or methyl-THF in the absence of the one or more exogenous proteins. For example, the microbial organism can contain at least one exogenous nucleic acid encoding an enzyme or protein in an acetyl-CoA pathway. The microbial organism is capable of utilizing synthesis gases comprising CO, CO2 and/or H2, alone or in combination with methanol, to produce acetyl-CoA. The invention additionally provides a method for producing acetyl-CoA, for example, by culturing an acetyl-CoA producing microbial organism, where the microbial organism expresses at least one exogenous nucleic acid encoding an acetyl-CoA pathway enzyme or protein in a sufficient amount to produce acetyl-CoA, under conditions and for a sufficient period of time to produce acetyl-CoA.