公开(公告)号：US5682884A

公开(公告)日：1997-11-04

申请号：US281131

申请日：1994-07-27

申请人： Hugh Allen Oliver Hill ,  Irving John Higgins ,  James Michael McCann ,  Graham Davis

发明人： Hugh Allen Oliver Hill ,  Irving John Higgins ,  James Michael McCann ,  Graham Davis

IPC分类号： A61B5/00 ,  C12Q1/00 ,  C12Q1/68 ,  G01N27/403 ,  G01N33/487 ,  G01N33/543 ,  A61B5/05

CPC分类号： A61B5/1486 ,  A61B5/14865 ,  A61B5/6848 ,  C12Q1/001 ,  C12Q1/004 ,  G01N27/4035 ,  G01N33/5438 ,  A61B2560/0418

摘要： A sensor system that detects a current representative of a compound in a liquid mixture features a two-electrode strip adapted for releasable attachment to signal readout circuitry. The strip comprises an elongated support (preferably flat) adapted for releasable attachment to the readout circuitry; a first conductor and a second conductor each extend along the support and comprise means for connection to the circuitry. An active electrode, positioned to contact the liquid mixture and the first conductor, comprises a deposit of an enzyme capable of catalyzing a reaction involving the compound and (preferably) an electron mediator, capable of transferring electrons between the enzyme-catalyzed reaction and the first conductor. A reference electrode is positioned to contact the mixture and the second conductor. The system includes circuitry adapted to provide an electrical signal representative of the current. The two-electrode strip is manufactured, e.g., by screen printing the enzyme (and preferably the mediator) onto the substrate.

摘要翻译： 检测液体混合物中的化合物的当前代表的传感器系统具有适于可释放地附接到信号读出电路的双电极条。 条带包括适于可释放地附接到读出电路的细长支撑件（优选是平坦的）; 第一导体和第二导体各自沿着支撑件延伸并且包括用于连接到电路的装置。 定位成接触液体混合物和第一导体的活性电极包括能够催化涉及化合物和（优选）电子介体的反应的酶的沉积物，其能够在酶催化的反应和第一个 导体。 定位参考电极以接触混合物和第二导体。 该系统包括适于提供代表电流的电信号的电路。 例如通过将酶（优选介体）丝网印刷到基底上来制造双电极条。

公开(公告)号：US5030571A

公开(公告)日：1991-07-09

申请号：US79759

申请日：1987-07-30

申请人： David J. Best ,  Peter A. Vaughan

发明人： David J. Best ,  Peter A. Vaughan

IPC分类号： C12N9/80 ,  C12R1/01

CPC分类号： C12R1/01 ,  C12N9/80

摘要： An aryl acylamidase is produced by a process which comprises culturing in a culture medium an aryl acylamidase-producing bacterial strain selected from Rhodococcus erythropolis NCIB 12273 and aryl acylamidase-producing mutants or variants thereof, and collecting aryl acylamidase-containing material.

摘要翻译： 通过包括在培养基中培养选自红球菌红细菌NCIB 12273的芳基酰氨基淀粉酶菌株和产生芳基酰基酰胺酶的突变体或其变体，并收集含芳基酰基酰胺酶的物质的方法制备芳基酰胺酶。

公开(公告)号：US4830959A

公开(公告)日：1989-05-16

申请号：US929332

申请日：1986-11-10

申请人： Calum J. McNeil ,  Joseph V. Bannister

发明人： Calum J. McNeil ,  Joseph V. Bannister

IPC分类号： G01N33/543 ,  G01N33/58

CPC分类号： G01N33/581 ,  G01N33/5438 ,  Y10S435/81 ,  Y10S435/817 ,  Y10S436/806 ,  Y10S436/904

摘要： Electrochemical detection of mediator level is employed in a method of assay using a redox enzyme and redox substrate to detect conversion to an effective mediator by an assay enzyme label of a compound which is non-mediating under the assay conditions.

摘要翻译： 在使用氧化还原酶和氧化还原底物的测定方法中使用介体水平的电化学检测来通过在测定条件下不介导的化合物的测定酶标记来检测到有效介质的转化。

公开(公告)号：US4777132A

公开(公告)日：1988-10-11

申请号：US847955

申请日：1986-04-03

申请人： Monika J. Green ,  Philip N. B. Gibbs

发明人： Monika J. Green ,  Philip N. B. Gibbs

IPC分类号： G01N27/327 ,  C12N9/02 ,  C12Q1/26 ,  G01N27/27 ,  G01N27/30 ,  G01N27/416 ,  G01N27/48

CPC分类号： C12N9/0073 ,  C12Q1/26 ,  C12Y114/13001 ,  Y10S435/817

摘要： The specification discloses a method and apparatus for the assay of salicylate (or a derivative thereof) comprising a conducting body having at a surface thereof an enzyme capable of catalyzing the conversion of salicylate (or a derivative thereof) to a catechol whereby said catechol is subject to direct electrochemical measurement as it oxidizes at said surface to generate a current as a measure of the reaction taking place and thereby of the concentration of salicylate at said surface. The method typically comprises the steps of;(a) treating a liquid sample suspected of containing salicylate or a derivative thereof with an enzyme capable of catalyzing the conversion of salicylate or a derivative thereof into a catechol, and,(b) measuring the concentration of catechol in the treated sample by direct electrochemistry.

摘要翻译： 本说明书公开了一种用于测定水杨酸（或其衍生物）的方法和装置，其包含导电体，其表面具有能够催化水杨酸酯（或其衍生物）转化为邻苯二酚的酶，由此所述儿茶酚是受试者 直接电化学测量，因为它在所述表面处氧化以产生电流，作为所发生的反应的测量，从而在所述表面处产生水杨酸盐的浓度。 该方法通常包括以下步骤： （a）用能够催化水杨酸酯或其衍生物转化成儿茶酚的酶处理怀疑含有水杨酸酯或其衍生物的液体样品，和（b）通过直接电化学测量处理的样品中的儿茶酚浓度 。

公开(公告)号：US5849174A

公开(公告)日：1998-12-15

申请号：US776716

申请日：1997-06-11

申请人： Gurdial Singh Sanghera ,  Philip Nigel Bartlett ,  Peter Robert Birkin

发明人： Gurdial Singh Sanghera ,  Philip Nigel Bartlett ,  Peter Robert Birkin

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/02 ,  G01N27/416 ,  G01N27/26

CPC分类号： C12Q1/001 ,  Y10S435/817

摘要： A method for indicating the concentration of a substance in solution includes passing an alternating voltage between a first electrode structure having coated thereon a polymer and a second separate counter electrode in the solution. The polymer is in one of an oxidized and a reduced state, between which states its conductivity varies. Changes in the conductivity of the polymer coating are measured, the measurement being representative of the state of the polymer and thereby of the concentration of the substance in the solution. Electrodes for use in this method are also described.

摘要翻译： PCT No.PCT / GB95 / 01818 Sec。 371日期：1997年6月11日 102（e）日期1997年6月11日PCT提交1995年8月1日PCT公布。 公开号WO96 / 04398 日期1996年2月15日用于指示溶液中物质浓度的方法包括在溶液中涂覆有聚合物的第一电极结构和第二分离的对电极之间传递交流电压。 聚合物处于氧化和还原状态之间，其中导电性变化。 测量聚合物涂层的电导率的变化，测量代表聚合物的状态，从而代表溶液中物质的浓度。 还描述了用于该方法的电极。

公开(公告)号：US5658444A

公开(公告)日：1997-08-19

申请号：US545805

申请日：1995-03-18

申请人： Murdo Black ,  Long Lin ,  James Guthrie

发明人： Murdo Black ,  Long Lin ,  James Guthrie

IPC分类号： B01D69/14 ,  C12Q1/00 ,  G01N27/327 ,  G01N27/40 ,  G01N33/49 ,  G01N33/50 ,  G01N27/26

CPC分类号： B01D69/06 ,  B01D69/141 ,  C12Q1/002 ,  G01N27/3272 ,  G01N33/5002 ,  G01N27/40 ,  Y10S435/817 ,  Y10S435/969 ,  Y10S435/97 ,  Y10S435/975

摘要： Erythrocyte exclusion membranes suited for test strips of electrochemical sensors have a porous matrix and a mobile erythrocyte aggregating agent. The membranes typically comprise a water-insoluble hydrophobic polymer incorporating a hydrophilic polymer and with the erythrocyte aggregating agent. Such membranes can have a pore size which is several times larger than the diameter of erythrocytes, and allow rapid passage of plasma by holding back the erythrocytes as rouleaux at the surface of the membrane. The membranes are preferably made by forming in situ on a test strip using spray casting.

摘要翻译： PCT No.PCT / GB94 / 01011 Sec。 371日期：1996年3月18日 102（e）1996年3月18日PCT PCT 1994年5月11日PCT公布。 公开号WO94 / 27140 日期1994年11月24日适用于电化学传感器测试条的红细胞排除膜具有多孔基质和可移动的红细胞聚集剂。 膜通常包含掺入亲水性聚合物和红细胞聚集剂的水不溶性疏水性聚合物。 这样的膜可以具有比红细胞直径大几倍的孔径，并且允许通过在红细胞的表面保持红细胞而快速通过血浆。 膜优选通过使用喷射浇铸在试纸上原位成型而制成。

公开(公告)号：US5628890A

公开(公告)日：1997-05-13

申请号：US534876

申请日：1995-09-27

申请人： Nigel F. Carter ,  Geoffrey R. Chambers ,  Graham J. Hughes ,  Steven Scott ,  Gurdial S. Sanghera ,  Jared L. Watkin

发明人： Nigel F. Carter ,  Geoffrey R. Chambers ,  Graham J. Hughes ,  Steven Scott ,  Gurdial S. Sanghera ,  Jared L. Watkin

IPC分类号： G01N27/327 ,  C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/001 ,  Y10S435/817

摘要： An electrode strip for use in an electrochemical sensor for measuring a compound in a sample is provided, including an electrode support, a reference or counter electrode disposed on the support, a working electrode spaced from the reference or counter electrode on the support, a covering layer defining an enclosed space over the reference and working electrodes and having an aperture for receiving a sample into the enclosed space, and a plurality of mesh layers interposed in the enclosed space between the covering layer and the support, the covering layer having a sample application aperture spaced from said electrodes and said reference electrode spaced from said working electrode at a position remote from and on the opposite side of said working electrode from said aperture. The working electrode includes an enzyme capable of catalyzing a reaction involving a substrate for the enzyme or a substrate catalytically reactive with an enzyme and a mediator capable of transferring electrons transferred between the enzyme-catalyzed reaction and the working electrode to create a current representative of the activity of the enzyme and representative of the compound.

摘要翻译： 提供了用于测量样品中化合物的电化学传感器的电极条，包括设置在支撑体上的电极支撑体，参考电极或对电极，与支撑体上的参考电极或对电极隔开的工作电极， 层限定在参考电极和工作电极上的封闭空间，并具有用于将样品接收到封闭空间中的孔，以及插入在覆盖层和支撑体之间的封闭空间中的多个网格层，覆盖层具有样品应用 与所述电极间隔开的孔，并且所述参考电极在远离所述工作电极的离开所述孔的位置处和与所述工作电极间隔开的位置处。 工作电极包括能够催化涉及用于酶的底物或与酶催化反应的底物的反应的酶和能够转移在酶催化反应和工作电极之间转移的电子的介体，以产生代表 酶的活性和化合物的代表。

公开(公告)号：US5264106A

公开(公告)日：1993-11-23

申请号：US33422

申请日：1993-03-18

申请人： Jerome F. McAleer ,  John T. Law ,  Richard A. Morris ,  Lesley Scott ,  John M. Mellor ,  Manus Dennison

发明人： Jerome F. McAleer ,  John T. Law ,  Richard A. Morris ,  Lesley Scott ,  John M. Mellor ,  Manus Dennison

IPC分类号： C12Q1/00 ,  G01N27/26

CPC分类号： C12Q1/001 ,  Y10S435/817

摘要： An amperometric sensor comprises a working electrode, a reference electrode, an enzyme whose catalytic activity on a substrate results in electroactivity, and an enhancer compound which enhances the detected electroactivity.

摘要翻译： 电流传感器包括工作电极，参比电极，其对底物的催化活性导致电活性的酶以及增强检测到的电活性的增强剂化合物。

公开(公告)号：US4948727A

公开(公告)日：1990-08-14

申请号：US786974

申请日：1985-10-11

申请人： Anthony E. G. Cass ,  Helena Bramwell

发明人： Anthony E. G. Cass ,  Helena Bramwell

IPC分类号： G01N27/416 ,  C12Q1/00 ,  G01N27/30 ,  G01N27/327 ,  G01N27/48 ,  G01N33/94

CPC分类号： C12Q1/005 ,  G01N33/9486 ,  Y10S435/817

摘要： The present invention is concerned with a chemical sensor, more specifically with a chemical sensor which is capable of detecting the presence of, measuring a quantity of or monitoring the level of N-acetyl primary aromatic amines, such as paracetamol, in whole blood.The specification relates to a method of assay of the type in which an electrode poised at a suitable potential is contacted with a system comprising;(a) a sample suspected to contain paracetamol or a derivative thereof, and(b) an enzyme capable of catalyzing the hydrolysis of an N-acylated primary aromatic amine or a derivative thereof, and,wherein the current flowing in the electrode is a measure of the quantity of hydrolysis products formed and thereby of the concentration of N-acylated primary aromatic amine or derivatives thereof in the sample.

摘要翻译： 本发明涉及一种化学传感器，更具体地涉及一种化学传感器，该化学传感器能够检测全血中N-乙酰基一级芳族胺如对乙酰氨基酚的量的量的测量或监测。 本说明书涉及一种测定其中将在适当电位下稳定的电极与包含以下系统的系统接触的类型的方法： （a）疑似含有对乙酰氨基酚或其衍生物的样品，和（b）能够催化N-酰化的伯芳香胺或其衍生物的水解的酶，并且其中在电极中流动的电流是度量 形成的水解产物的量，从而得到样品中N-酰化的伯芳族胺或其衍生物的浓度。

公开(公告)号：US4876205A

公开(公告)日：1989-10-24

申请号：US84983

申请日：1987-08-12

申请人： Monika J. Green ,  Roger J. Lias

发明人： Monika J. Green ,  Roger J. Lias

IPC分类号： G01N27/416 ,  G01N27/49 ,  G01N33/487 ,  G01N33/72

CPC分类号： G01N33/721

摘要： A method for the assay of haemoglobin in blood is provided in which the blood is contacted with a sufficient amount of a ferricyanide and the haemoglobin is electrochemically assayed by monitoring the current changes produced on reduction of the ferricyanide by the haemoglobin.

摘要翻译： 提供血液中血红蛋白测定方法，其中血液与足量的铁氰化物接触，血红蛋白通过监测由血红蛋白还原铁氰化物产生的当前变化进行电化学测定。