公开(公告)号：US11988665B2

公开(公告)日：2024-05-21

申请号：US17800296

申请日：2021-02-26

申请人： Analiza, Inc.

发明人： Arnon Chait ,  Boris Y. Zaslavsky

IPC分类号： G01N33/569 ,  C12Q1/6888 ,  C12Q1/70 ,  G01N33/49

CPC分类号： G01N33/56983 ,  C12Q1/6888 ,  C12Q1/70 ,  G01N33/491 ,  G01N2333/11 ,  G01N2333/165

摘要： Certain aspects of the present disclosure generally relate to systems and methods for determining viruses. For instance, some aspects are directed to systems and methods for determining viruses using a partitioning system. Within the partitioning system, the virus may partition into one or more phases. In some cases, a virus-binding moiety facilitates partitioning of the virus. The phases may be assayed to determine the virus based on, e.g., quantitative or qualitative assessments of the distribution of virus-binding and/or signaling moieties. The virus-binding moiety may be attached to particles that may form a complex around a virus. The complex may be detectable without a signaling moiety (e.g., as a color change) in some embodiments. In some cases, more than one virus may be determined. For example, a virus-binding moiety may substantially alter the partitioning behavior of one virus or complex, relative to another, by being selective for the first virus.

公开(公告)号：US20240002959A1

公开(公告)日：2024-01-04

申请号：US18240748

申请日：2023-08-31

申请人： Analiza, Inc.

发明人： Arnon Chait ,  Boris Y. Zaslavsky

IPC分类号： C12Q1/70 ,  A61K9/51 ,  A61K39/215 ,  G01N33/52 ,  G01N33/569

CPC分类号： C12Q1/701 ,  A61K9/51 ,  A61K39/215 ,  G01N33/523 ,  G01N33/56983 ,  G01N2333/165

摘要： Certain aspects of the present disclosure generally relate to systems and methods for determining viruses such as coronaviruses. For instance, some aspects are directed to systems and methods for determining viruses using a partitioning system. Within the partitioning system, free RNA or other nucleic acids may preferentially partition into one phase, while intact viruses may be present in the other phase or in both phases. Accordingly, in some cases, free RNA or other nucleic acids may be preferentially removed, e.g., as compared to intact RNA or other nucleic acids present within a virus. In some cases, the phase containing intact viruses can be determined to determine the infectiousness, e.g., of a sample arising from a subject. This may be useful, for example, for distinguishing subjects who are capable of spreading an infection from those who are not infectious.

公开(公告)号：US20230314439A1

公开(公告)日：2023-10-05

申请号：US18030849

申请日：2022-10-13

申请人： Analiza, Inc.

发明人： Arnon Chait ,  Boris Y. Zaslavsky

IPC分类号： G01N33/574 ,  G01N33/50

CPC分类号： G01N33/57488 ,  G01N33/5002

摘要： The present disclosure generally relates to systems and methods for partitioning species. In some embodiments, such systems can be used for determining one or more types of cancer. For example, certain aspects are generally directed to aqueous multi-phase partitioning systems that can be used, for example, for distinguishing between different types of cancers, diagnosing subjects with cancer, or the like. In some cases, such systems can be identified using solvent properties such as the solvent dipolarity/polarizability difference (Δπ*), the solvent hydrogen bond donor acidity difference (Δα), the solvent hydrogen bond acceptor basicity difference (Δβ), and the electrostatic property difference (c) between two phases of the partitioning system. These may be within certain ranges in accordance with various embodiments. Additionally, these ranges can be precisely tuned by controlling the compositions of the phases of the partitioning system to produce novel partitioning systems that are unexpectedly efficient at distinguishing between even relatively minor structural changes of proteins or other species, which accordingly can be used to distinguishing different types of cancers, or for other applications.

公开(公告)号：US11422135B2

公开(公告)日：2022-08-23

申请号：US16273253

申请日：2019-02-12

申请人： Analiza, Inc.

发明人： Arnon Chait ,  Boris Y. Zaslavsky

IPC分类号： G01N33/48 ,  G01N33/50 ,  G01N33/574 ,  G16B5/00 ,  G16C20/30 ,  G01N33/53 ,  G01N33/68 ,  G16H50/50 ,  G01N33/543 ,  G16B20/00

摘要： The present invention generally provides systems and methods for the detection, identification, or characterization of differences between properties or behavior of corresponding species in two or more mixtures comprised of molecules, including biomolecules and/or molecules able to interact with biomolecules, using techniques such as partitioning. The experimental conditions established as distinguishing between the mixtures of the molecules using the systems and methods of the invention can also be used, in some cases, for further fractionation and/or characterization of the biomolecules and/or other molecules, using techniques such as single-step or multiple-step extraction, and/or by liquid-liquid partition chromatography. The methods could also be used for discovering and identifying markers associated with specific diagnostics, and can be used for screening for such markers once discovered and identified during diagnostics screening.

公开(公告)号：US20190178901A1

公开(公告)日：2019-06-13

申请号：US16208671

申请日：2018-12-04

申请人： Analiza, Inc.

发明人： Pavel Idelevich ,  Arnon Chait ,  Alan Bauer ,  Paul David Jackson

IPC分类号： G01N33/84 ,  C12Q1/6806 ,  G01N33/50 ,  G01N33/569 ,  G01N33/574

摘要： Some embodiments of the present invention generally relate to devices and methods for determining and/or isolating cells. For example, one aspect is generally directed to methods and devices for detecting, identifying, counting, and/or potentially sorting cells of interest in blood or other biological sample. In some embodiments, blood samples (or other biological fluids) may be treated with signaling entities, such as pH-sensitive entities, that change color or otherwise produce a signal in suitable internal environments. For example, certain cells, such as cancer or fetal cells, may have differences in intracellular pH compared to other cells, which can be detected using pH-sensitive entities. In certain embodiments, the cells may be sorted based on such signaling entities; for example, illumination of cells in a suitable machine for sorting cells (e.g., using fluorescent light) may allow determination of the cells, which may also be recovered or isolated for further manipulation in some cases.

公开(公告)号：US11896748B2

公开(公告)日：2024-02-13

申请号：US16617904

申请日：2018-06-01

申请人： University of Massachusetts ,  Analiza, Inc. ,  Medical University of Graz

发明人： Yossi Chait ,  Joseph Horowitz ,  Christopher V. Hollot ,  Hamed Samandari ,  Michael J. Germain ,  Daniel Schneditz

IPC分类号： A61M1/16 ,  A61B5/02 ,  A61B5/145 ,  A61M1/36 ,  A61M1/26 ,  A61B5/0275 ,  A61B5/00

CPC分类号： A61M1/1613 ,  A61B5/02028 ,  A61B5/0275 ,  A61B5/14535 ,  A61B5/6866 ,  A61M1/267 ,  A61M1/361 ,  A61M2205/3389 ,  A61M2205/50 ,  A61M2205/52 ,  A61M2230/20 ,  A61M2230/207

摘要： Absolute blood volume in dialysis patients is a useful patient attribute to know for dialysis treatment, diagnosis, adjustments, etc. In some cases, it is difficult or impossible to directly determine absolute blood volume. Estimating absolute blood volume may be used to overcome the inability to directly determine the absolute blood volume. Estimating absolute blood volume may include obtaining a series of measurements of hemoconcentration of a patient over a time period, and estimating parameters for a physiological model based on the series of measurements. The absolute blood volume of the patient may be determined using the physiological model.

公开(公告)号：US20220365074A1

公开(公告)日：2022-11-17

申请号：US17212080

申请日：2021-03-25

申请人： Analiza, Inc.

发明人： Alan Bauer ,  Arnon Chait

IPC分类号： G01N33/50 ,  G01N33/52 ,  G01N15/14

摘要： Certain aspects of the present disclosure generally relates to devices and methods for determining, treating, and/or isolating cells of interest, e.g., within a mixture of cells. In some cases, the cells may be cells infected with viruses, such as coronaviruses. In some embodiments, blood samples (or other biological fluids, such as saliva) may be treated with a pH-sensitive entity. The pH-sensitive entity may be one that is able to change color or otherwise produce a signal in suitable internal environments. For example, cells infected by viruses, such as coronaviruses, may have differences in intracellular pH compared to other cells, which can be detected, for example, using pH-sensitive entities. In certain embodiments, the cells may be sorted based on such signaling entities; for example, illumination of cells in a suitable machine for sorting cells (e.g., using fluorescent light) may allow determination of the cells, which may also be recovered or isolated for further manipulation in some cases.

公开(公告)号：US20190178891A1

公开(公告)日：2019-06-13

申请号：US16273253

申请日：2019-02-12

申请人： Analiza, Inc.

发明人： Arnon Chait ,  Boris Y. Zaslavsky

IPC分类号： G01N33/574 ,  G01N33/68 ,  G01N33/53 ,  G16C20/30 ,  G16B5/00 ,  G16H50/50 ,  G01N33/543

摘要： The present invention generally provides systems and methods for the detection, identification, or characterization of differences between properties or behavior of corresponding species in two or more mixtures comprised of molecules, including biomolecules and/or molecules able to interact with biomolecules, using techniques such as partitioning. The experimental conditions established as distinguishing between the mixtures of the molecules using the systems and methods of the invention can also be used, in some cases, for further fractionation and/or characterization of the biomolecules and/or other molecules, using techniques such as single-step or multiple-step extraction, and/or by liquid-liquid partition chromatography. The methods could also be used for discovering and identifying markers associated with specific diagnostics, and can be used for screening for such markers once discovered and identified during diagnostics screening.

公开(公告)号：US09709556B2

公开(公告)日：2017-07-18

申请号：US14225512

申请日：2014-03-26

申请人： Analiza, Inc.

发明人： Pavel Idelevich ,  Arnon Chait ,  Alan Bauer

IPC分类号： G01N33/50 ,  G01N33/574

CPC分类号： G01N33/5094 ,  G01N33/5005 ,  G01N33/5091 ,  G01N33/57492 ,  G01N2333/70514 ,  G01N2333/70517

摘要： The present invention generally relates to devices and methods for determining and/or isolating cells. One aspect is generally directed to methods and devices for detecting, identifying, counting, and/or potentially sorting cells of interest in blood or other biological sample. In some embodiments, blood samples (or other biological fluids) may be treated with signaling entities, such as pH-sensitive entities, that change color or otherwise produce a signal in suitable internal environments. For example, certain cells, such as cancer or fetal cells, may have differences in intracellular pH compared to other cells, which can be detected using pH-sensitive entities. In certain embodiments, the cells may be sorted based on such signaling entities; for example, illumination of cells in a suitable machine for sorting cells (e.g., using fluorescent light) may allow determination of the cells, which may also be recovered or isolated for further manipulation in some cases.

公开(公告)号：US20150369807A1

公开(公告)日：2015-12-24

申请号：US14312907

申请日：2014-06-24

申请人： Analiza, Inc.

发明人： Arnon Chait ,  Boris Y. Zaslavsky

IPC分类号： G01N33/574 ,  G01N33/68

CPC分类号： G01N33/57488 ,  G01N33/54366 ,  G01N33/57415 ,  G01N33/68 ,  G01N33/6893 ,  G01N2333/765 ,  G01N2800/085 ,  G01N2800/56

摘要： The present invention, in some embodiments thereof, relates to methods and devices for determining a disease state in a patient. In some embodiments, human serum albumin may be analyzed for associated molecules, wherein the associated molecules are related to a disease such as cancer. Certain aspects of the invention are generally directed to devices and methods for determining a disease state as a function of the three-dimensional structure of a blood protein, human serum albumin (HSA) or its complex with other ligands, e.g., due to binding to disease-specific ligands. HSA interacts with biomolecules associated with a disease presence, and liganded HSA may differentially partition between aqueous phases of a predetermined partitioning system, wherein the partitioning behavior differs between HSA from healthy individuals and HSA from people harboring the predetermined disease, such as breast cancer.

摘要翻译： 本发明在其一些实施例中涉及用于确定患者疾病状态的方法和装置。 在一些实施方案中，可以分析人血清白蛋白相关分子，其中相关分子与诸如癌症的疾病相关。 本发明的某些方面通常涉及用于确定作为血液蛋白质，人血清白蛋白（HSA）或其与其它配体的复合物的三维结构的函数的疾病状态的装置和方法，例如由于与 疾病特异性配体。 HSA与与疾病存在相关的生物分子相互作用，并且配体HSA可以在预定分配系统的水相之间差异性地分配，其中分配行为在HSA与健康个体之间不同，HSA与携带预定疾病的人（例如乳腺癌）不同。