GLUCOAMYLASE VARIANTS
    16.
    发明申请

    公开(公告)号:US20110014681A1

    公开(公告)日:2011-01-20

    申请号:US12680193

    申请日:2008-04-08

    IPC分类号: C12N9/34 C07H21/00 C12N1/15

    摘要: The present invention relates to glucoamylase variants. In particular, the invention relates to variants in the starch binding domain (SBD) of a glucoamylase. The invention also relates to variants having altered properties (e.g., improved thermostability and/or increased specific activity) as compared to a corresponding parent glucoamylase. The present invention also provides enzyme compositions comprising the variant glucoamylases; DNA constructs comprising polynucleotides encoding the variants; and methods of producing the glucoamylase variants in host cells.

    摘要翻译: 本发明涉及葡糖淀粉酶变体。 特别地,本发明涉及葡糖淀粉酶的淀粉结合结构域(SBD)中的变体。 本发明还涉及与相应的母体葡糖淀粉酶相比具有改变的性质(例如,改善的热稳定性和/或增加的比活性)的变体。 本发明还提供了包含变体葡糖淀粉酶的酶组合物; 包含编码变体的多核苷酸的DNA构建体; 以及在宿主细胞中产生葡糖淀粉酶变体的方法。

    Pullulanase variants with increased productivity
    20.
    发明授权
    Pullulanase variants with increased productivity 失效
    支链淀粉酶变体具有更高的生产力

    公开(公告)号:US08354101B2

    公开(公告)日:2013-01-15

    申请号:US13036749

    申请日:2011-02-28

    IPC分类号: A61K38/46 C12N9/44

    CPC分类号: C12Y302/01041 C12N9/2457

    摘要: The invention relates to novel variants of the enzymatic peptide pullulanase, the gene sequences encoding said novel peptides, expression vectors comprising those gene sequences as well as organisms expressing the novel pullulanase variants. The novel pullulanase variants of the present invention were made empirically by the use of codon-optimization procedures, selective truncation of “wild-type” molecules and through the replacement of selected amino acid residues. Furthermore, the invention relates to the use of these novel pullulanase peptides in the textile, fermentation, food and other industries.

    摘要翻译: 本发明涉及酶促支链淀粉酶的新变体,编码所述新肽的基因序列,包含那些基因序列的表达载体以及表达新型支链淀粉酶变体的生物。 通过使用密码子优化程序,野生型分子的选择性截短和通过置换所选择的氨基酸残基,经验地制备了本发明的新型支链淀粉酶变体。 此外,本发明涉及这些新型支链淀粉酶肽在纺织,发酵,食品和其它工业中的用途。