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公开(公告)号：US5548661A
公开(公告)日：1996-08-20
申请号：US302044
申请日：1994-09-07
申请人： Jeffrey H. Price , David Gough
发明人： Jeffrey H. Price , David Gough
CPC分类号： G06T7/0083 , G01N15/147 , G01N21/6428 , G01N21/6458 , G06K9/00127 , G06T7/0012 , G06T7/0095 , G06T2207/10056 , G06T2207/10064 , G06T2207/30024
摘要： An operator independent image cytometer having a method for image segmentation. Image segmentation comprises the steps of filtering a digital image of a cellular specimen and thresholding the resultant image. In addition, the thresholding may include the sorting of features extracted from the filtered image. The present invention also includes a method for cytometer autofocus that combines the benefits of sharpening and contrast metrics. The present invention further includes an arc lamp stabilization and intensity control system. The image cytometer has broad applications in determining DNA content and other cellular measurements on as many as 10.sup.5 individual cells, including specimens of living cells. Image segmentation applications include PAP smear analysis and particle recognition.
摘要翻译： 具有用于图像分割的方法的操作者独立的图像细胞计数器。 图像分割包括对细胞样本的数字图像进行过滤并对所得到的图像进行阈值化的步骤。 此外，阈值处理可以包括从滤波图像提取的特征的排序。 本发明还包括一种结合了锐化和对比度量度的优点的用于细胞仪自动对焦的方法。 本发明还包括弧光灯稳定和亮度控制系统。 图像细胞仪在确定多达105个个体细胞（包括活细胞标本）的DNA含量和其他细胞测量中具有广泛的应用。 图像分割应用包括PAP涂片分析和粒子识别。
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公开(公告)号：US20180307005A1
公开(公告)日：2018-10-25
申请号：US16011610
申请日：2018-06-18
CPC分类号： G02B7/38 , G02B13/08 , G02B21/242 , G02B21/365 , H04N5/2258 , H04N5/23212 , H04N9/09
摘要： Multifunction autofocus for automated microscopy includes automatic coarse focusing of an automated microscope by reflective positioning, followed by automatic image-based autofocusing of the automated microscope performed in reference to a coarse focus position. In some aspects, the image-based autofocusing utilizes astigmatism in microscope optics for multi-planar image acquisition along a Z axis direction of a microscope. In some other aspects, the image-based autofocusing utilizes astigmatism in microscope optics in combination with chromatic aberration for multi-planar image acquisition along the Z axis direction.
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13.发明授权Automated scanning cytometry using chromatic aberration for multiplanar image acquisition 失效自动扫描细胞仪使用色差进行多平面图像采集公开(公告)号：US08760756B2
公开(公告)日：2014-06-24
申请号：US12587923
申请日：2009-10-14
IPC分类号： G02B21/00
CPC分类号： G02B21/0016 , G02B21/006 , G02B21/0064 , G02B21/244
摘要： In an automated scanning cytometry system, chromatic aberration is used for multiplanar image acquisition.
摘要翻译： 在自动扫描细胞计数系统中，色差用于多平面图像采集。
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公开(公告)号：US20130244318A1
公开(公告)日：2013-09-19
申请号：US13775034
申请日：2013-02-22
IPC分类号： C12Q1/02
CPC分类号： C12Q1/02 , G06T7/0016 , G06T2207/10016 , G06T2207/10056 , G06T2207/10064 , G06T2207/20152 , G06T2207/20216 , G06T2207/30024
摘要： A method, system, and instrument for automatically measuring transient activity in cells uses image time sequences to identify transients in cells. Preferably, the transient activity is stimulated or provoked in synchronism with acquisition of the image time sequences. A cell mask is applied to each image of an image time sequence in order to localize the transient activity with respect to each cell. Localization enables cell-by-cell analysis of properties of the transient activity.
摘要翻译： 用于自动测量细胞中瞬时活动的方法，系统和仪器使用图像时间序列来识别细胞中的瞬变。 优选地，瞬时活动与获取图像时间序列同步地被刺激或激发。 将细胞掩模应用于图像时间序列的每个图像，以便相对于每个细胞定位瞬时活动。 本地化使得能够逐个细胞地分析瞬时活动的性质。
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公开(公告)号：US20130100272A1
公开(公告)日：2013-04-25
申请号：US13317727
申请日：2011-10-25
CPC分类号： G02B7/38 , G02B13/08 , G02B21/242 , G02B21/365 , H04N5/2258 , H04N5/23212 , H04N9/09
摘要： Multifunction autofocus for automated microscopy includes automatic coarse focusing of an automated microscope by reflective positioning, followed by automatic image-based autofocusing of the automated microscope performed in reference to a coarse focus position. In some aspects, the image-based autofocusing utilizes astigmatism in microscope optics for multi-planar image acquisition along a Z axis direction of a microscope. In some other aspects, the image-based autofocusing utilizes astigmatism in microscope optics in combination with chromatic aberration for multi-planar image acquisition along the Z axis direction.
摘要翻译： 用于自动显微镜的多功能自动对焦包括通过反射定位对自动显微镜进行自动粗略对焦，然后根据粗焦点位置执行自动显微镜的自动图像自动对焦。 在某些方面，基于图像的自动对焦利用显微镜光学中的像散，沿着显微镜的Z轴方向进行多平面图像采集。 在一些其他方面，基于图像的自动对焦利用显微镜光学中的散光以及沿着Z轴方向进行多平面图像采集的色像差。
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16.发明申请公开(公告)号：US20110274337A1
公开(公告)日：2011-11-10
申请号：US13066712
申请日：2011-04-21
CPC分类号： G06K9/00127 , G01N15/1475 , G06T7/12 , G06T7/155 , G06T2207/10056 , G06T2207/10064 , G06T2207/20152 , G06T2207/30024
摘要： A system, method and kit for processing an original image of biological material to identify certain components of a biological object by locating the biological object in the image, enhancing the image by sharpening components of interest in the object, and applying a contour-finding function to the enhanced image to create a contour mask. The contour mask may be processed to yield a segmented image divided by structural units of the biological material.
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公开(公告)号：US20100289887A1
公开(公告)日：2010-11-18
申请号：US12454217
申请日：2009-05-13
CPC分类号： C12Q1/02 , G06T7/0016 , G06T2207/10016 , G06T2207/10056 , G06T2207/10064 , G06T2207/20152 , G06T2207/20216 , G06T2207/30024
摘要： A method, system, and instrument for automatically measuring transient activity in cells uses image time sequences to identify transients in cells. Preferably, the transient activity is stimulated or provoked in synchronism with acquisition of the image time sequences. A cell mask is applied to each image of an image time sequence in order to localize the transient activity with respect to each cell. Localization enables cell-by-cell analysis of properties of the transient activity.
摘要翻译： 用于自动测量细胞中瞬时活动的方法，系统和仪器使用图像时间序列来识别细胞中的瞬变。 优选地，瞬时活动与获取图像时间序列同步地被刺激或激发。 将细胞掩模应用于图像时间序列的每个图像，以便相对于每个细胞定位瞬时活动。 本地化使得能够逐个细胞地分析瞬时活动的性质。
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18.发明申请Automated scanning cytometry using chromatic aberrtation for multiplanar image acquisition 失效自动扫描细胞计数使用色差进行多平面图像采集公开(公告)号：US20100172020A1
公开(公告)日：2010-07-08
申请号：US12587923
申请日：2009-10-14
IPC分类号： G02B21/00
CPC分类号： G02B21/0016 , G02B21/006 , G02B21/0064 , G02B21/244
摘要： In an automated scanning cytometry system, chromatic aberration is used for multiplanar image acquisition.
摘要翻译： 在自动扫描细胞计数系统中，色差用于多平面图像采集。
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公开(公告)号：US5856665A
公开(公告)日：1999-01-05
申请号：US858293
申请日：1997-05-19
申请人： Jeffrey H. Price , David Gough
发明人： Jeffrey H. Price , David Gough
CPC分类号： G06T7/0083 , G01N15/147 , G01N21/6428 , G01N21/6458 , G06K9/00127 , G06T7/0012 , G06T7/0095 , G06T2207/10056 , G06T2207/10064 , G06T2207/30024
摘要： An operator independent image cytometer having a method for image segmentation. Image segmentation comprises the steps of filtering a digital image of a cellular specimen and thresholding the resultant image. In addition, the thresholding may include the sorting of features extracted from the filtered image. The present invention also includes a method for cytometer autofocus that combines the benefits of sharpening and contrast metrics. The present invention further includes an arc lamp stabilization and intensity control system. The image cytometer has broad applications in determining DNA content and other cellular measurements on as many as 10.sup.5 individual cells, including specimens of living cells. Image segmentation applications include PAP smear analysis and particle recognition.
摘要翻译： 具有用于图像分割的方法的操作者独立的图像细胞计数器。 图像分割包括对细胞样本的数字图像进行过滤并对所得到的图像进行阈值化的步骤。 此外，阈值处理可以包括从滤波图像提取的特征的排序。 本发明还包括一种结合了锐化和对比度量度的优点的用于细胞仪自动对焦的方法。 本发明还包括弧光灯稳定和亮度控制系统。 图像细胞仪在确定多达105个个体细胞（包括活细胞标本）的DNA含量和其他细胞测量中具有广泛的应用。 图像分割应用包括PAP涂片分析和粒子识别。
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20.发明授权Method and means of least squares designed filters for image segmentation in scanning cytometry 失效用于扫描细胞计数的图像分割的最小二乘法设计滤波器的方法和装置
公开(公告)号：US5790692A
公开(公告)日：1998-08-04
申请号：US820442
申请日：1997-03-12
申请人： Jeffrey H. Price , David A. Gough
发明人： Jeffrey H. Price , David A. Gough
CPC分类号： G06T7/0081 , G01N15/147 , G06K9/00127 , G06T7/0012 , G06T2207/10056 , G06T2207/10064 , G06T2207/20148 , G06T2207/30024
摘要： In an image segmentation system that processes image objects by digital filtration, a digital filter is defined. The digital filter includes a neighborhood operator for processing intensity values of neighborhoods of pixels in a pixel array. A first pixel array is received defining a pixelated image including one or more objects and a background and a second pixel array is received that defines a reference image. The reference image includes at least one object included in the pixelated image in a background. In the reference image, pixels included in the at least one object are distinguished from pixels included in the background by a predetermined amount of contrast. Pixels of the first and second images are compared to determine a merit value; the merit value is used to compute neighborhood operator values; and, the neighborhood operator is applied to images in order to create or enhance contrast between objects and background in the images.
摘要翻译： 在通过数字滤波处理图像对象的图像分割系统中，定义了数字滤波器。 数字滤波器包括用于处理像素阵列中的像素的邻域的强度值的邻域运算符。 接收第一像素阵列，其定义包括一个或多个对象和背景的像素化图像，并且接收限定参考图像的第二像素阵列。 参考图像包括背景中包括在像素化图像中的至少一个对象。 在参考图像中，将包括在至少一个对象中的像素与包含在背景中的像素区分预定量的对比度。 比较第一和第二图像的像素以确定优值; 优点值用于计算邻域运算符值; 并且，邻域运算符被应用于图像以便创建或增强图像中的对象和背景之间的对比度。
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