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公开(公告)号：US09315847B2
公开(公告)日：2016-04-19
申请号：US13775034
申请日：2013-02-22
CPC分类号： C12Q1/02 , G06T7/0016 , G06T2207/10016 , G06T2207/10056 , G06T2207/10064 , G06T2207/20152 , G06T2207/20216 , G06T2207/30024
摘要： A method, system, and instrument for automatically measuring transient activity in cells uses image time sequences to identify transients in cells. Preferably, the transient activity is stimulated or provoked in synchronism with acquisition of the image time sequences. A cell mask is applied to each image of an image time sequence in order to localize the transient activity with respect to each cell. Localization enables cell-by-cell analysis of properties of the transient activity.
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公开(公告)号：US07756305B2
公开(公告)日：2010-07-13
申请号：US10351455
申请日：2003-01-23
申请人： Jeffrey H. Price
发明人： Jeffrey H. Price
IPC分类号： G06K9/00
CPC分类号： G01N21/6458 , G01N15/1475 , G01N2015/0065 , G02B21/367 , G06T5/004 , G06T5/50 , Y10S128/922
摘要： Methods and apparatus are described for enhancing operator independent image cytometry. Aspects of the invention include enhanced tissue surface tracking which can detect both tissue surfaces. The surface detection can aid cytometry, such as by reducing the amount of image data to be stored. Segmentation is described for 3D images in which 3D least squares filtering is applied to increase contrast for simplifying the delineation of objects from backgrounds. A method of creating 3D FIR image filters based on ideal objects is also described. A data structure is defined by which 3D object data may be organized for image representations if samples. Methods of performing remote segmentation processing are also described toward centralizing the necessary processor power and applications and reducing the burden on researchers and clinicians.
摘要翻译： 描述了用于增强操作者独立的图像细胞计数的方法和装置。 本发明的方面包括能够检测组织表面的增强的组织表面跟踪。 表面检测可以帮助细胞计数，例如通过减少要存储的图像数据的量。 针对3D图像描述了分割，其中应用3D最小二乘滤波以增加对比度以简化对象从背景的描绘。 还描述了基于理想对象创建3D FIR图像滤波器的方法。 定义数据结构，通过哪个3D对象数据可以被组织用于图像表示，如果样本。 还描述了执行远程分割处理的方法，以集中所需的处理器功能和应用，并减轻研究人员和临床医师的负担。
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公开(公告)号：USRE40674E1
公开(公告)日：2009-03-24
申请号：US10000943
申请日：2001-11-30
IPC分类号： H04N5/202
CPC分类号： G02B21/244
摘要： An analog circuit for an autofocus microscope system measures a degree of focus of an object directly from the video signal of a microscope CCD camera. The circuit then returns an index to a host computer for the purpose of adjusting the position of the microscope's objective lens to bring the object in focus. Best focus is found by comparing indices at several different vertical positions. The criterion adopted for determining the degree of focus is derived from the energy distribution of the video signal spectrum. The high frequency energy of the video spectrum is a maximum at best focus and as the optics defocus, the distribution shifts to lower frequencies. Low cost, real time autofocus is achieved with the analog circuitry of this invention, replacing more expensive dedicated, real time image processing hardware.
摘要翻译： 用于自动对焦显微镜系统的模拟电路直接从显微镜CCD照相机的视频信号测量物体的焦点。 电路然后将索引返回到主计算机，目的是调整显微镜物镜的位置以使对象聚焦。 通过比较几个不同垂直位置的指标，找到最佳的焦点。 用于确定焦点度的标准是从视频信号频谱的能量分布中得出的。 视频频谱的高频能量在最佳对焦下是最大的，并且随着光学散焦，分布向低频转移。 采用本发明的模拟电路实现了低成本的实时自动对焦，代替更昂贵的专用的实时图像处理硬件。
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公开(公告)号：US07358478B2
公开(公告)日：2008-04-15
申请号：US11433049
申请日：2006-05-12
申请人： Jeffrey H. Price
发明人： Jeffrey H. Price
IPC分类号： H01J3/14
CPC分类号： G02B21/22
摘要： A system for imaging of a sample is disclosed. The system includes a plurality of detectors which are each focussed at a respective focal plane in a sample volume. The system also includes light selection optics positioned between the plurality of detectors and the sample volume. The light selection optics transmit to the detectors a portion of light originating at the respective focal planes while screening out light which originates from outside of the respective focal planes.
摘要翻译： 公开了一种用于成像样本的系统。 该系统包括多个检测器，每个检测器被聚焦在样品体积中的相应焦平面处。 该系统还包括位于多个检测器和样品体积之间的光选择光学器件。 光选择光学器件向检测器传送源自各个焦平面的光的一部分，同时屏蔽来自各个焦平面外部的光。
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5.发明授权Automatic on-the-fly focusing for continuous image acquisition in high-resolution microscopy 失效自动即时聚焦，用于高分辨率显微镜中的连续图像采集公开(公告)号：US06640014B1
公开(公告)日：2003-10-28
申请号：US09235215
申请日：1999-01-22
IPC分类号： H04N718
CPC分类号： G02B21/241
摘要： The invention provides an apparatus, and related method, for continuous volume imaging a specimen using imaging a plurality of image planes at predetermined focal depths. The focal depth for each image plane is adjustable in accordance with a variety of desired spatial configurations. The images generated at the image planes are processing in parallel and the image corresponding the an optimal focal depth at an specimen location is selected for imaging the specimen at that location. The volume imaging apparatus of the invention allows for rapid scanning of an entire microscope slide at high image resolution.
摘要翻译： 本发明提供了一种用于使用在预定焦点深度成像多个图像平面来对样本进行连续体积成像的装置和相关方法。 每个图像平面的焦深可根据各种所需的空间配置进行调节。 在图像平面生成的图像被并行处理，并且选择与样本位置处的最佳焦点深度相对应的图像以在该位置成像样本。 本发明的体积成像装置允许以高图像分辨率快速扫描整个显微镜载玻片。
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公开(公告)号：US5995143A
公开(公告)日：1999-11-30
申请号：US796196
申请日：1997-02-07
CPC分类号： G02B21/244
摘要： An analog circuit for an autofocus microscope system measures a degree of focus of an object directly from the video signal of a microscope CCD camera. The circuit then returns an index to a host computer for the purpose of adjusting the position of the microscope's objective lens to bring the object in focus. Best focus is found by comparing indices at several different vertical positions. The criterion adopted for determining the degree of focus is derived from the energy distribution of the video signal spectrum. The high frequency energy of the video spectrum is a maximum at best focus and as the optics defocus, the distribution shifts to lower frequencies. Low cost, real time autofocus is achieved with the analog circuitry of this invention, replacing more expensive dedicated, real time image processing hardware.
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7.发明授权Autofocus system for scanning microscopy having a volume image formation 失效用于扫描具有体积图像形成的显微镜的自动对焦系统
公开(公告)号：US5932872A
公开(公告)日：1999-08-03
申请号：US913647
申请日：1997-09-10
申请人： Jeffrey H. Price
发明人： Jeffrey H. Price
CPC分类号： G02B21/244 , G01N15/147 , G02B21/002
摘要： Reliable autofocus is required to obtain accurate measurements of fluorescent stained cellular components from a system capable of scanning multiple microscope fields. Autofocus could be performed directly with fluorescence images, but due to photobleaching and destructive fluorescence by-products, it is best to minimize fluorescence exposure for photosensitive specimens and live cells. This exposure problem could be completely avoided by using phase-contrast microscopy, implemented through the same optics as fluorescence microscopy. Functions for both phase-contrast and fluorescence autofocus were evaluated using the present invention and the suitability of phase-contrast autofocus for fluorescence microscopy was determined. The present autofocus system for scanning microscopy can be performed at least as fast as 0.25 s/field without loss of precision. The speed of autofocus can be further increased by a volume image which is obtained by observing an image object at each image plane of a plurality of image planes, where each image plane is vertically displaced with respect to each other image plane. An electronic image representation is obtained at each image plane. The image planes are scanned over the image object and the images arm aligned timewise in a buffer. The buffer holds a volume image comprising images at the image planes, aligned by the buffer. The image plane having the best focus is selected and a microscope objective is automatically positioned at the selected plane.
摘要翻译： PCT No.PCT / US95 / 08424 Sec。 371日期1998年1月20日 102（e）日期1998年1月20日PCT提交1995年6月30日PCT公布。 公开号WO96 / 01438 日期1996年1月18日需要进行可靠的自动对焦以从能够扫描多个显微镜领域的系统获得荧光染色的细胞成分的精确测量。 自动对焦可以直接用荧光图像进行，但由于光漂白和破坏性荧光副产物，最好使感光标本和活细胞的荧光曝光最小化。 通过使用通过与荧光显微镜相同的光学器件实现的相差显微术可以完全避免这种暴露问题。 使用本发明评价相位对比度和荧光自动聚焦的功能，并确定相位自动对焦自动对焦用于荧光显微镜的适用性。 目前用于扫描显微镜的自动对焦系统可以至少快达0.25秒/场，而不会损失精度。 可以通过在多个图像平面的每个图像平面处观察图像对象而获得的体积图像来进一步增加自动对焦速度，其中每个图像平面相对于彼此图像平面垂直移位。 在每个图像平面处获得电子图像表示。 图像平面在图像对象上扫描，图像臂在缓冲器中按时间对齐。 缓冲器保存包括图像平面处的图像的体积图像，由缓冲器对准。 选择具有最佳焦点的图像平面，并且显微镜物镜被自动定位在所选择的平面上。
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公开(公告)号：US10001622B2
公开(公告)日：2018-06-19
申请号：US13317727
申请日：2011-10-25
CPC分类号： G02B7/38 , G02B13/08 , G02B21/242 , G02B21/365 , H04N5/2258 , H04N5/23212 , H04N9/09
摘要： Multifunction autofocus for automated microscopy includes automatic coarse focusing of an automated microscope by reflective positioning, followed by automatic image-based autofocusing of the automated microscope performed in reference to a coarse focus position. In some aspects, the image-based autofocusing utilizes astigmatism in microscope optics for multi-planar image acquisition along a Z axis direction of a microscope. In some other aspects, the image-based autofocusing utilizes astigmatism in microscope optics in combination with chromatic aberration for multi-planar image acquisition along the Z axis direction.
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9.发明申请System, method, and kit for processing a magnified image of biological material to identify components of a biological object 有权用于处理生物材料的放大图像以识别生物体的组分的系统，方法和试剂盒公开(公告)号：US20080144895A1
公开(公告)日：2008-06-19
申请号：US11285691
申请日：2005-11-21
CPC分类号： G06K9/00127 , G01N15/1475 , G06T7/12 , G06T7/155 , G06T2207/10056 , G06T2207/10064 , G06T2207/20152 , G06T2207/30024
摘要： A system, method and kit for processing an original image of biological material to identify certain components of a biological object by locating the biological object in the image, enhancing the image by sharpening components of interest in the object, and applying a contour-finding function to the enhanced image to create a contour mask. The contour mask may be processed to yield a segmented image divided by structural units of the biological material.
摘要翻译： 一种用于处理生物材料的原始图像以通过将生物体放置在图像中来识别生物体的某些组分的系统，方法和试剂盒，通过锐化对象中感兴趣的成分来增强图像，以及应用轮廓寻找功能 到增强图像以创建轮廓掩模。 轮廓掩模可以被处理以产生分割的图像，除以生物材料的结构单元。
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公开(公告)号：US5790710A
公开(公告)日：1998-08-04
申请号：US785614
申请日：1997-01-17
申请人： Jeffrey H. Price , David A. Gough
发明人： Jeffrey H. Price , David A. Gough
CPC分类号： G02B21/244 , G01N15/147 , G02B21/002
摘要： Reliable autofocus is required to obtain accurate measurements of fluorescent stained cellular components from a system capable of scanning multiple microscope fields. Autofocus could be performed directly with fluorescence images, but due to photobleaching and destructive fluorescence by-products, it is best to minimize fluorescence exposure for photosensitive specimens and live cells. This exposure problem could be completely avoided by using phase-contrast microscopy, implemented through the same optics as fluorescence microscopy. Functions for both phase-contrast and fluorescence autofocus were evaluated using the present invention and the suitability of phase-contrast autofocus for fluorescence microscopy was determined. The present autofocus system for scanning microscopy can be performed at least as fast as 0.25 s/field without loss of precision.
摘要翻译： 需要可靠的自动对焦以从能够扫描多个显微镜领域的系统获得荧光染色的细胞成分的精确测量。 自动对焦可以直接用荧光图像进行，但由于光漂白和破坏性荧光副产物，最好使感光标本和活细胞的荧光曝光最小化。 通过使用通过与荧光显微镜相同的光学器件实现的相差显微术可以完全避免这种暴露问题。 使用本发明评价相位对比度和荧光自动聚焦的功能，并确定相位自动对焦自动对焦用于荧光显微镜的适用性。 目前用于扫描显微镜的自动对焦系统可以至少快达0.25秒/场，而不会损失精度。
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