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    Translocation and mutant ROS kinase in human non-small cell lung carcinoma
    11.
    发明授权
    Translocation and mutant ROS kinase in human non-small cell lung carcinoma 有权
    人非小细胞肺癌易位和突变型ROS激酶

    公开(公告)号：US09096855B2

    公开(公告)日：2015-08-04

    申请号：US12738210

    申请日：2008-10-20

    Applicant: Ting-Lei Gu ,  Ailan Guo

    Inventor： Ting-Lei Gu ,  Ailan Guo

    IPC: C12Q1/68 ,  C12N15/11 ,  C12N15/12 ,  C12N15/113 ,  C07K14/74 ,  C07K14/82 ,  C07K16/40 ,  C12N9/99 ,  A61K39/00

    CPC classification number: C12Q1/6886 ,  A61K2039/505 ,  C07K14/70539 ,  C07K14/82 ,  C07K16/40 ,  C07K2319/00 ,  C12N9/12 ,  C12N9/99 ,  C12N15/1137 ,  C12Q1/68 ,  C12Q1/6813 ,  C12Q1/6883 ,  C12Q2600/136 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Y207/10001

    Abstract: In accordance with the invention, a novel gene translocation, (5q32, 6q22), in human non-small cell lung carcinoma (NSCLC) that results in a fusion proteins combining part of CD74 with Proto-oncogene Tyrosine Protein Kinase ROS Precursor (ROS) kinase has now been identified. The CD74-ROS fusion protein is anticipated to drive the proliferation and survival of a subgroup of NSCLC tumors. The invention therefore provides, in part, isolated polynucleotides and vectors encoding the disclosed mutant ROS kinase polypeptides, probes for detecting it, isolated mutant polypeptides, recombinant polypeptides, and reagents for detecting the fusion and truncated polypeptides. The disclosed identification of the new fusion protein enables new methods for determining the presence of these mutant ROS kinase polypeptides in a biological sample, methods for screening for compounds that inhibit the proteins, and methods for inhibiting the progression of a cancer characterized by the mutant polynucleotides or polypeptides, which are also provided by the invention.

    Abstract translation: 根据本发明，在人非小细胞肺癌（NSCLC）中的新基因易位（5q32,6q22），其导致将CD74的一部分与原癌基因酪氨酸蛋白激酶ROS前体（ROS）结合的融合蛋白， 激酶现已被鉴定。 预期CD74-ROS融合蛋白将驱动NSCLC肿瘤亚组的增殖和存活。 因此，本发明部分地提供分离的多核苷酸和编码所公开的突变型ROS激酶多肽的载体，用于检测其的探针，分离的突变多肽，重组多肽和用于检测融合和截短的多肽的试剂。 所公开的新融合蛋白的鉴定使得能够确定生物样品中这些突变型ROS激酶多肽的存在的新方法，用于筛选抑制蛋白质的化合物的方法，以及抑制以突变体多核苷酸为特征的癌症进展的方法 或多肽，其也由本发明提供。

    Reagent and kit for classifying and counting leukocytes, the preparation thereof, and process for classifying and counting leukocytes
    12.
    发明授权
    Reagent and kit for classifying and counting leukocytes, the preparation thereof, and process for classifying and counting leukocytes 有权
    用于白细胞分类和计数的试剂和试剂盒，其制备方法以及白细胞分类和计数方法

    公开(公告)号：US08685661B2

    公开(公告)日：2014-04-01

    申请号：US12843671

    申请日：2010-07-26

    Applicant: Yang Zhao ,  Ting Lei ,  Yumei Zhao ,  Bing Xu

    Inventor： Yang Zhao ,  Ting Lei ,  Yumei Zhao ,  Bing Xu

    IPC: G01N33/49 ,  A61K31/427

    CPC classification number: G01N15/147 ,  G01N33/56972 ,  G01N2015/008 ,  G01N2015/1477 ,  G01N2015/1486

    Abstract: A reagent for classifying and counting leukocytes containing (1) a cyanine fluorescent dye; and (2) a glycoside compound; a reagent kit containing the reagent for classifying and counting leukocytes as well as its preparation process; and a process for classifying and counting blood cells using the reagent or kit are provided. Using the reagent, kit and/or process provided, leukocytes can be classified and counted in four groups with a high degree of differentiation and a better classification among each subpopulation of leukocytes, especially in that it successfully addresses the indistinct classification between lymphocytes and monocytes and between the eosinophils and neutrophils in a scattergram.

    Abstract translation: 一种用于对含有（1）花青荧光染料的白细胞进行分类和计数的试剂; 和（2）糖苷化合物; 含有白细胞分类和计数试剂的试剂盒及其制备方法; 并提供使用试剂或试剂盒对血细胞进行分类和计数的方法。 使用所提供的试剂，试剂盒和/或方法，可以将白细胞分类和计数在四个组中，在白细胞的每个亚群中具有高度分化和更好的分类，特别是其成功地解决了淋巴细胞和单核细胞之间的模糊分类， 嗜中性粒细胞和嗜中性粒细胞之间。

    GENE DEFECTS AND MUTANT ALK KINASE IN HUMAN SOLID TUMORS
    13.
    发明申请
    GENE DEFECTS AND MUTANT ALK KINASE IN HUMAN SOLID TUMORS 审中-公开
    人类固体肿瘤中的基因缺陷和突变碱性激酶

    公开(公告)号：US20130209452A1

    公开(公告)日：2013-08-15

    申请号：US13618400

    申请日：2012-09-14

    Applicant: Klarisa Rikova ,  Herbert Haack ,  Laura Sullivan ,  Ailan Guo ,  Anthony Possemato ,  Joan MacNeill ,  Ting-Lei Gu ,  Jian Yu

    Inventor： Klarisa Rikova ,  Herbert Haack ,  Laura Sullivan ,  Ailan Guo ,  Anthony Possemato ,  Joan MacNeill ,  Ting-Lei Gu ,  Jian Yu

    IPC: C12Q1/68 ,  A61K31/713 ,  G01N33/68

    CPC classification number: C12Q1/6886 ,  A61K31/713 ,  A61K38/00 ,  C07K14/47 ,  C07K2319/00 ,  C12N9/12 ,  C12N9/1205 ,  C12Q2600/112 ,  C12Q2600/156 ,  C12Q2600/158 ,  C12Y207/10001 ,  G01N33/57423 ,  G01N33/57484 ,  G01N33/6893 ,  G01N2333/912 ,  G01N2333/9121

    Abstract: Novel gene deletions and translocations involving chromosome 2 resulting in fusion proteins combining part of Anaplastic Lymphoma Kinase (ALK) kinase with part of a secondary protein have been identified herein in human solid tumors, e.g. non-small cell lung carcinoma (NSCLC). Secondary proteins include Echinoderm Microtubule-Associated Protein-Like 4 (EML-4) and TRK-Fusion Gene (TFG). The EML4-ALK fusion protein, which retains ALK tyrosine kinase activity, was confirmed to drive the proliferation and survival of NSCLC characterized by this mutation. The invention therefore provides, in part, isolated polynucleotides and vectors encoding the disclosed mutant ALK kinase polypeptides, probes for detecting it, isolated mutant polypeptides, recombinant polypeptides, and reagents for detecting the fusion and truncated polypeptides. The disclosed identification of this new fusion protein enables methods for screening for compounds that inhibit the proteins, and methods for inhibiting the progression of a cancer characterized by the mutant polynucleotides or polypeptides.

    Abstract translation: 涉及染色体2的新型基因缺失和易位导致融合蛋白结合部分间变型淋巴瘤激酶（ALK）激酶与部分二级蛋白，已经在人体实体肿瘤中被鉴定，例如， 非小细胞肺癌（NSCLC）。 次级蛋白包括棘皮动物微管相关蛋白样4（EML-4）和TRK-融合基因（TFG）。 确认了保留ALK酪氨酸激酶活性的EML4-ALK融合蛋白，以驱动以这种突变为特征的NSCLC的增殖和存活。 因此，本发明部分地提供分离的多核苷酸和编码所公开的突变ALK激酶多肽的载体，用于检测其的探针，分离的突变多肽，重组多肽和用于检测融合和截短的多肽的试剂。 所公开的这种新的融合蛋白的鉴定使得能够筛选抑制蛋白质的化合物的方法，以及抑制以突变多核苷酸或多肽为特征的癌症进展的方法。

    REAGENT AND KIT FOR CLASSIFYING AND COUNTING LEUKOCYTES, THE PREPARATION THEREOF, AND PROCESS FOR CLASSIFYING AND COUNTING LEUKOCYTES
    15.
    发明申请
    REAGENT AND KIT FOR CLASSIFYING AND COUNTING LEUKOCYTES, THE PREPARATION THEREOF, AND PROCESS FOR CLASSIFYING AND COUNTING LEUKOCYTES 有权
    用于分类和计数白藜芦醇的试剂和试剂盒，其制备方法和分类和计数白藜芦醇的方法

    公开(公告)号：US20110027788A1

    公开(公告)日：2011-02-03

    申请号：US12843671

    申请日：2010-07-26

    Applicant: Yang Zhao ,  Ting Lei ,  Yumei Zhao ,  Bing Xu

    Inventor： Yang Zhao ,  Ting Lei ,  Yumei Zhao ,  Bing Xu

    IPC: C12Q1/68

    CPC classification number: G01N15/147 ,  G01N33/56972 ,  G01N2015/008 ,  G01N2015/1477 ,  G01N2015/1486

    Abstract: A reagent for classifying and counting leukocytes containing (1) a cyanine fluorescent dye; and (2) a glycoside compound; a reagent kit containing the reagent for classifying and counting leukocytes as well as its preparation process; and a process for classifying and counting blood cells using the reagent or kit are provided. Using the reagent, kit and/or process provided, leukocytes can be classified and counted in four groups with a high degree of differentiation and a better classification among each subpopulation of leukocytes, especially in that it successfully addresses the indistinct classification between lymphocytes and monocytes and between the eosinophils and neutrophils in a scattergram.

    Abstract translation: 一种用于对含有（1）花青荧光染料的白细胞进行分类和计数的试剂; 和（2）糖苷化合物; 含有白细胞分类和计数试剂的试剂盒及其制备方法; 并提供使用试剂或试剂盒对血细胞进行分类和计数的方法。 使用所提供的试剂，试剂盒和/或方法，可以将白细胞分类和计数在四个组中，在白细胞的每个亚群中具有高度分化和更好的分类，特别是其成功地解决了淋巴细胞和单核细胞之间的模糊分类， 嗜中性粒细胞和嗜中性粒细胞之间。

    Reagents for the detection of protein phosphorylation in signaling pathways
    16.
    发明申请
    Reagents for the detection of protein phosphorylation in signaling pathways 审中-公开
    用于检测信号通路中蛋白质磷酸化的试剂

    公开(公告)号：US20100159477A1

    公开(公告)日：2010-06-24

    申请号：US12309311

    申请日：2007-07-13

    Applicant: Peter Hornbeck ,  Valerie Goss ,  Kimberly Lee ,  Ting-Lei Gu ,  Albrecht Moritz

    Inventor： Peter Hornbeck ,  Valerie Goss ,  Kimberly Lee ,  Ting-Lei Gu ,  Albrecht Moritz

    IPC: G01N33/573 ,  G01N33/53 ,  C07K16/00 ,  C07K7/06 ,  C07K7/08 ,  C07K14/00 ,  C12N5/071 ,  C12N5/16 ,  C12N5/18

    CPC classification number: G01N33/68 ,  C12Q1/485 ,  G01N33/6842 ,  G01N33/6845 ,  G01N33/6848 ,  G01N33/6872

    Abstract: The invention discloses novel phosphorylation sites identified in signal transduction proteins and pathways, and provides phosphorylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these phosphorylated sites/proteins, as well as methods of using the reagents for such purpose. Among the phosphorylation sites identified are sites occurring in the following protein types: adaptor/scaffold proteins, adhesion/extracellular matrix protein, apoptosis proteins, calcium binding proteins, cell cycle regulation proteins, chaperone proteins, chromatin, DNA binding/repair/replication proteins, cytoskeletal proteins, endoplasmic reticulum or golgi proteins, enzyme proteins, G/regulator proteins, inhibitor proteins, motor/contractile proteins, phosphatase, protease, Ser/Thr protein kinases, Protein kinase (Tyr)s, receptor/channel/cell suface proteins, RNA binding proteins, transcriptional regulators, tumor suppressor proteins, ubiquitan conjugating system proteins and proteins of unknown function.

    Abstract translation: 本发明公开了在信号转导蛋白和途径中鉴定的新型磷酸化位点，并提供磷酸化位点特异性抗体和重同位素标记肽（AQUA肽），用于选择性检测和定量这些磷酸化位点/蛋白质，以及使用 用于此目的的试剂。 鉴定的磷酸化位点是发生在以下蛋白质类型中的位点：衔接子/支架蛋白，粘附/细胞外基质蛋白，凋亡蛋白，钙结合蛋白，细胞周期调节蛋白，伴侣蛋白，染色质，DNA结合/修复/复制蛋白， 细胞骨架蛋白，内质网或高尔基体蛋白，酶蛋白，G /调节蛋白，抑制蛋白，运动/收缩蛋白，磷酸酶，蛋白酶，Ser / Thr蛋白激酶，蛋白激酶（Tyr），受体/通道/细胞表面蛋白， RNA结合蛋白，转录调节物，肿瘤抑制蛋白，泛素偶联系统蛋白和功能未知的蛋白。

    Reagens for the Detection of Protein Acetylation Signaling Pathways
    20.
    发明申请
    Reagens for the Detection of Protein Acetylation Signaling Pathways 审中-公开
    用于检测蛋白质乙酰化信号通路的试剂

    公开(公告)号：US20090124023A1

    公开(公告)日：2009-05-14

    申请号：US12227321

    申请日：2007-05-11

    Applicant: Ailan Guo ,  Ting-Lei Gu ,  Jeffrey Mitchell ,  Peter Hornbeck

    Inventor： Ailan Guo ,  Ting-Lei Gu ,  Jeffrey Mitchell ,  Peter Hornbeck

    IPC: G01N33/566 ,  C07K16/18

    CPC classification number: G01N33/68 ,  C07K16/18 ,  C07K16/44 ,  G01N33/6842

    Abstract: The invention discloses 432 novel acetylation sites identified in signal transduction proteins and pathways underlying human protein acetylation signaling pathways, and provides acetylation-site specific antibodies and heavy-isotope labeled peptides (AQUA peptides) for the selective detection and quantification of these acetylated sites/proteins, as well as methods of using the reagents for such purpose. Among the acetylation sites identified are sites occurring in the following protein types: Acetyltransferases, Adaptor/Scaffold proteins, Actin binding proteins, Adhesion proteins, Apoptosis proteins, Calcium-binding proteins, Cell Cycle Regulation proteins, Cell Surface proteins, DNA binding proteins, DNA replication proteins, Channel proteins, Chaperone proteins, Cellular Metabolism enzymes, Cytoskeletal proteins, DNA repair proteins, Endoplasmic reticulum proteins, Enzyme proteins, G protein and GTPase Activating proteins, Guanine Nucleotide Exchange Factors, Helicase proteins, Isomerase proteins, Extracelluar matrix proteins, Hydrolases, Ligase proteins, Lipid kinases, Inhibtor proteins, Lipid Binding proteins and Lyases.

    Abstract translation: 本发明公开了在信号转导蛋白中鉴定的432个新的乙酰化位点和基于人蛋白质乙酰化信号通路的途径，并提供乙酰化位点特异性抗体和重同位素标记肽（AQUA肽），用于选择性检测和定量这些乙酰化位点/蛋白质 ，以及使用试剂用于此目的的方法。 确定的乙酰化位点之间的位点是发生在以下蛋白质类型中的位点：乙酰转移酶，适配器/支架蛋白，肌动蛋白结合蛋白，粘附蛋白，细胞凋亡蛋白，钙结合蛋白，细胞周期调节蛋白，细胞表面蛋白，DNA结合蛋白，DNA 复制蛋白，通道蛋白，伴侣蛋白，细胞代谢酶，细胞骨架蛋白，DNA修复蛋白，内质网蛋白，酶蛋白，G蛋白和GTP酶活化蛋白，鸟嘌呤核苷酸交换因子，Helicase蛋白，异构酶蛋白，Extracelluar基质蛋白，水解酶 ，连接酶蛋白，脂质激酶，抑制蛋白，脂质结合蛋白和裂解酶。

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