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30 条记录 (耗时 0.017 秒)

    Chemiluminescence enhancer
    11.
    发明授权
    Chemiluminescence enhancer 有权
    化学发光增强剂

    公开(公告)号：US07276343B2

    公开(公告)日：2007-10-02

    申请号：US10518586

    申请日：2003-06-24

    申请人： Kazushige Moriyama ,  Tetsuji Tanimoto ,  Tatsuki Matsuno ,  Yoshihiro Ashihara

    发明人： Kazushige Moriyama ,  Tetsuji Tanimoto ,  Tatsuki Matsuno ,  Yoshihiro Ashihara

    IPC分类号： G01N33/53 ,  G01N33/542 ,  G01N33/543 ,  G01N33/553 ,  G01N33/566 ,  G01N21/75 ,  G01N21/76 ,  C12Q1/00 ,  C12N9/00 ,  C09K3/00

    CPC分类号： G01N33/533 ,  G01N21/76 ,  G01N33/532

    摘要： The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.

    摘要翻译： 本发明提供一种化学发光增强剂，其处理以保持细固体载体的良好分散性并稳定地发挥化学发光增强作用。 本发明提供了一种化学发光增强剂，其用于固相免疫测定中的信号检测，所述抗原或/和抗体固定在可分散于液体介质中的细小固体载体上，该水溶液由水溶性大分子季铵盐，季锍盐或季鏻 盐，以增强由具有二氧杂环丁烷的化学发光底物的酶反应引起的光的发射，其中通过用氧化剂或还原剂处理化学发光增强剂对细小固体载体进行聚集抑制处理，以及化学发光 方法和使用化学发光增强剂的试剂盒。

    Liquid supply device and analytical test device
    12.
    发明授权
    Liquid supply device and analytical test device 失效
    液体供应装置和分析试验装置

    公开(公告)号：US6017494A

    公开(公告)日：2000-01-25

    申请号：US65725

    申请日：1998-04-24

    申请人： Yoshihiro Ashihara ,  Mitsushi Gotanda ,  Akira Hasegawa ,  Yuko Fujiwara

    发明人： Yoshihiro Ashihara ,  Mitsushi Gotanda ,  Akira Hasegawa ,  Yuko Fujiwara

    IPC分类号： G01N31/22 ,  B01L3/00 ,  G01N1/10 ,  G01N33/543 ,  G01N33/558 ,  G01N35/02 ,  G01N21/29

    CPC分类号： G01N33/558 ,  B01L3/5023 ,  B01L2300/0672 ,  B01L2300/0825 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0683

    摘要： A liquid supply device for transporting a liquid to a predetermined portion includes a liquid container for holding a liquid therein, which has an opening, a sealing sheet member which is impermeable but rupturable and seals the opening of the container, and an absorbent material which is capable of transporting the liquid by capillary action, the absorbent material being disposed on the sealing sheet member, out of contact with the liquid when the sealing sheet member is not ruptured, while when the sealing sheet member is ruptured and the absorbent material is brought into contact with the liquid, part of the absorbent material being continuously held within the liquid and transporting the liquid to the predetermined position. An analytical test device using the above liquid supply device is provided, which may be used, for instance, immunoassay.

    摘要翻译： 用于将液体输送到预定部分的液体供应装置包括：具有开口的用于保持液体的液体容器，具有不可渗透但可破裂并且密封容器的开口的密封片部件;以及吸收材料， 能够通过毛细作用输送液体，当密封片构件未破裂时，吸收材料设置在密封片构件上，与液体不接触，而当密封片构件破裂并且吸收材料被引入时 与液体接触，吸收材料的一部分连续地保持在液体内并将液体输送到预定位置。 提供了使用上述液体供给装置的分析测试装置，其可以用于例如免疫测定。

    Method for determining the activity of the angiotensin-converting enzyme
    14.
    发明授权
    Method for determining the activity of the angiotensin-converting enzyme 失效
    测定血管紧张素转换酶活性的方法

    公开(公告)号：US4407944A

    公开(公告)日：1983-10-04

    申请号：US330110

    申请日：1981-12-14

    申请人： Yasushi Kasahara ,  Yoshihiro Ashihara

    发明人： Yasushi Kasahara ,  Yoshihiro Ashihara

    IPC分类号： G01N33/50 ,  C12Q1/34 ,  C12Q1/37 ,  C12Q1/36

    CPC分类号： C12Q1/37 ,  C12Q1/34 ,  C12Q2326/96 ,  C12Q2337/00

    摘要： A method for determining the activity of an angiotensin-converting enzyme is disclosed comprising the steps of mixing a liquid containing (i) a synthetic substrate X-hippuryl-L-dipeptide having the formula ##STR1## wherein X represents OH, NH.sub.2 or N(CH.sub.3).sub.2, and when R.sub.1 is H, R.sub.2 is H, and when R.sub.1 is ##STR2## R.sub.2 is CH.sub.2 CH(CH.sub.3).sub.2, (ii) hippuricase and (iii) 4-aminoantipyrine, with a liquid containing an angiotensin-converting enzyme; measuring colorimetrically the concentration of the quinonimine dye which is formed by adding an oxiding agent to the above mixture; and calculating the activity of the angiotensin-converting enzyme from the concentration of the quinonimine dye.

    摘要翻译： 公开了一种用于测定血管紧张素转换酶的活性的方法，包括以下步骤：混合含有（i）具有式“IMAGE”的合成底物X-马尿基-L-二肽的液体，其中X表示OH，NH 2或N（ CH 3）2，当R 1为H时，R 2为H，当R 1为CH 2 CH（CH 3）2时，（ⅱ）马尿酸和（iii）4-氨基安替比林与含有血管紧张素转化酶的液体 ; 通过向上述混合物中加入氧化剂形成的醌亚胺染料的浓度进行比色测定; 并从醌亚胺染料的浓度计算血管紧张素转换酶的活性。

    Method of measuring biological ligand by utilizing amylase
    17.
    发明授权
    Method of measuring biological ligand by utilizing amylase 失效
    利用淀粉酶测定生物配体的方法

    公开(公告)号：US4757001A

    公开(公告)日：1988-07-12

    申请号：US699675

    申请日：1985-02-08

    申请人： Yoshihiro Ashihara ,  Yasushi Kasahara

    发明人： Yoshihiro Ashihara ,  Yasushi Kasahara

    IPC分类号： G01N33/542 ,  G01N33/563

    CPC分类号： G01N33/563 ,  G01N33/542

    摘要： The present invention relates to the method of measuring a biological ligand (1) in a sample containing an amylase (1) derived from a higher animal. This method is characterized by employing a combination of an amylase (2) not contained in the sample and an antibody against the ligand (1) or a ligand (2) capable of reacting with the antibody and an amylase inhibitor of which inhibitory activity against the amylase (1) is stronger than that against the amylase (2). The method of the invention utilizes the emphasis of steric hindrance of the amylase (2) reaction which is carried out at the boundary between solid phase and liquid phase and the specific removal of the influence of the amylase (1) by using the above amylase inhibitor. This method is suitable as a clinical test for the determination of physiological substances and trace components in a humoral fluid such as serum.

    摘要翻译： 本发明涉及含有来自较高级动物的淀粉酶（1）的样品中的生物配体（1）的测定方法。 该方法的特征在于使用样品中不含有的淀粉酶（2）和配体（1）的抗体或能够与抗体反应的配体（2）和对该抗体具有抑制活性的淀粉酶抑制剂的组合 淀粉酶（1）比对淀粉酶（2）更强。 本发明的方法利用在固相和液相边界处进行的淀粉酶（2）反应的空间位阻的强调，以及通过使用上述淀粉酶抑制剂特异性去除淀粉酶（1）的影响 。 该方法适用于测定体液如血清中生理物质和痕量成分的临床试验。

    Method of measuring biological ligand by the use of enzymes
    18.
    发明授权
    Method of measuring biological ligand by the use of enzymes 失效
    通过使用酶测量生物配体的方法

    公开(公告)号：US4692404A

    公开(公告)日：1987-09-08

    申请号：US670764

    申请日：1984-11-13

    申请人： Yoshihiro Ashihara ,  Yasushi Kasahara

    发明人： Yoshihiro Ashihara ,  Yasushi Kasahara

    IPC分类号： G01N33/535 ,  G01N33/542 ,  G01N33/573 ,  G01N33/58 ,  G01N33/74 ,  C12Q1/34 ,  C12Q1/40 ,  G01N33/536

    CPC分类号： G01N33/542 ,  G01N33/535 ,  G01N33/573 ,  G01N33/581 ,  G01N33/74 ,  Y10S435/963 ,  Y10S435/97 ,  Y10S436/813 ,  Y10S436/815

    摘要： A method is provided which is highly sensitive and specific, and is suitable as a clinical test for the determination of physiological substances and trace components in humoral fluid. The method employs a conjugate of an enzyme capable of acting on a water-insoluble macromolecular substance and an antibody against the ligand (1) to be measured or a ligand (2) having an antigenic determinant common to one of the antigenic determinant(s) of the ligand (1). In the method of the invention, steric hindrance of the conjugate is emphasized by the enzyme reaction which is carried out at the boundary between solid phase and liquid phase.

    摘要翻译： 提供了一种高灵敏度和特异性的方法，适用于测定体液中生理物质和微量成分的临床试验。 该方法使用能够作用于水不溶性大分子物质的酶和针对要测量的配体（1）的抗体的缀合物或具有抗原决定簇之一共有的抗原决定簇的配体（2） 的配体（1）。 在本发明的方法中，通过在固相和液相之间的边界处进行的酶反应来强调缀合物的空间位阻。