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公开(公告)号：US06594432B2
公开(公告)日：2003-07-15
申请号：US09791994
申请日：2001-02-22
Applicant: Shiping Chen , Yuling Luo
Inventor： Shiping Chen , Yuling Luo
IPC: G02B600
CPC classification number: G01N21/6458 , B01J19/0046 , B01J2219/00369 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00628 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00673 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/0262 , B01L3/0268 , B01L3/563 , B01L2200/021 , B01L2300/0819 , B01L2300/0829 , B01L2400/022 , B01L2400/027 , C40B40/06 , C40B40/10 , C40B60/14 , G01N21/6452 , G01N35/1074 , G01N2021/6484 , G01N2035/1037
Abstract: Disclosed is a microarray printing system and methods of printing probe microarrays. The system has a print head formed of one or more bundles of individual capillaries, such as light-guiding capillaries. The bundles may especially be random bundles of capillaries that provide a large number of probes on the surface of a substrate. Methods of registering or correlating the distal and proximal ends of the capillaries are also provided. Further, the invention provides methods and equipment for identifying defective microarrays that are missing one or more probes from the surface of the microarray.
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公开(公告)号：US08628918B2
公开(公告)日：2014-01-14
申请号：US11431092
申请日：2006-05-08
Applicant: Yuling Luo , Wen Yang
Inventor： Yuling Luo , Wen Yang
CPC classification number: C12Q1/6837 , C12Q1/6834 , C12Q2565/543 , C12Q2537/143 , C12Q2537/125 , C12Q2565/515 , C12Q2527/107 , C12Q2563/149 , C12Q2563/143
Abstract: Methods of capturing two or more nucleic acids simultaneously from a single sample are provided. Different nucleic acids are captured through cooperative hybridization events on different subsets of particles or at different selected positions on a spatially addressable solid support. Compositions, kits, and systems related to the methods are also described.
Abstract translation: 提供了从单个样品同时捕获两种或更多种核酸的方法。 通过在不同的颗粒子集上或在空间上可寻址的固体支持物上的不同选择位置上的协同杂交事件捕获不同的核酸。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US08426578B2
公开(公告)日：2013-04-23
申请号：US12804904
申请日：2010-07-29
Applicant: Yuling Luo , Wen Yang
Inventor： Yuling Luo , Wen Yang
CPC classification number: C12Q1/6837 , C12Q1/682 , C12Q1/6834 , C12Q2537/143 , C12Q2537/125 , C12Q2525/313 , C12Q2565/543 , C12Q2565/501 , C12Q2563/149 , C12Q2563/103
Abstract: Methods of detecting two or more nucleic acids in a multiplex branched-chain DNA assay are provided. Different nucleic acids are captured through cooperative hybridization events on different, identifiable subsets of particles or at different selected positions on a spatially addressable solid support. Compositions, kits, and systems related to the methods are also described.
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公开(公告)号：US20130023433A1
公开(公告)日：2013-01-24
申请号：US13498249
申请日：2010-09-28
Applicant: Yuling Luo , John James Flanagan , Nan Su , Huei-Yu Fay Wang
Inventor： Yuling Luo , John James Flanagan , Nan Su , Huei-Yu Fay Wang
CPC classification number: C12Q1/6841 , C12Q2565/543 , C12Q2525/313
Abstract: The invention relates to methods of detecting nucleic acids, including methods of detecting one or more target nucleic acid sequences in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support or suspending cells are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. The invention further relates to methods to improve probe hybridization specificity and their application in genotyping. The invention also relates to in situ detection of mis-joined nucleic acid sequences. The invention relates to reducing false positive signals and improve signal-to-background ratio in hybridization-based nucleic acid detection assay. The invention further relates to method to improve specificity in hybridization based nucleic acid using co-location probes. Compositions, tissue slides, sample of suspended cells, kits, and systems related to the methods are also described.
Abstract translation: 本发明涉及检测核酸的方法，包括在多重支链DNA测定中检测一种或多种靶核酸序列的方法。 检测到在固体支持物或悬浮细胞上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。 本发明还涉及改进探针杂交特异性及其在基因分型中的应用的方法。 本发明还涉及错误连接的核酸序列的原位检测。 本发明涉及在基于杂交的核酸检测测定中减少假阳性信号并提高信号与背景比。 本发明还涉及使用共位点探针改进基于杂交的核酸的特异性的方法。 还描述了组合物，组织载玻片，悬浮细胞样品，试剂盒和与该方法相关的系统。
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公开(公告)号：US07927798B2
公开(公告)日：2011-04-19
申请号：US11543752
申请日：2006-10-04
Applicant: Zhi Zheng , Yuling Luo , Gary McMaster
Inventor： Zhi Zheng , Yuling Luo , Gary McMaster
CPC classification number: C12Q1/6816 , C12Q1/6837 , C12Q2537/143 , C12Q2537/125 , C12Q2525/313
Abstract: Methods of detecting one or more nucleic acids from whole blood or plasma are provided. The nucleic acids are captured on a solid support and detected. Compositions, kits, and systems related to the methods are also described.
Abstract translation: 提供从全血或血浆中检测一种或多种核酸的方法。 将核酸捕获在固体支持物上并检测。 还描述了与方法相关的组合物，试剂盒和系统。
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Patent Agency Ranking
公开(公告)号：US20110059442A1
公开(公告)日：2011-03-10
申请号：US12660524
申请日：2010-02-26
Applicant: Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
Inventor： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
IPC: C12Q1/68
CPC classification number: C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
Abstract: Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.
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17.发明申请Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations 审中-公开检测单个细胞中的核酸并鉴定来自大异质细胞群体的稀有细胞的方法公开(公告)号：US20080038725A1
公开(公告)日：2008-02-14
申请号：US11471278
申请日：2006-06-19
Applicant: Yuling Luo , Shiping Chen
Inventor： Yuling Luo , Shiping Chen
IPC: C12Q1/68
CPC classification number: C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
Abstract: Methods of detecting multiple nucleic acid targets in single cells through indirect capture of labels to the nucleic acids are provided. Methods of assaying the relative levels of nucleic acid targets through normalization to levels of reference nucleic acids are also provided. Methods of detecting individual cells, particularly rare cells from large heterogeneous cell populations, through detection of nucleic acids are described. Related compositions, systems, and kits are also provided.
Abstract translation: 提供了通过间接捕获标记到核酸来检测单个细胞中的多个核酸靶的方法。 还提供了通过归一化到参考核酸水平来测定核酸靶的相对水平的方法。 描述了通过检测核酸检测单个细胞，特别是来自大异质细胞群体的罕见细胞的方法。 还提供了相关的组合物，系统和试剂盒。
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公开(公告)号：US20070161015A1
公开(公告)日：2007-07-12
申请号：US11543752
申请日：2006-10-04
Applicant: Zhi Zheng , Yuling Luo , Gary McMaster
Inventor： Zhi Zheng , Yuling Luo , Gary McMaster
CPC classification number: C12Q1/6816 , C12Q1/6837 , C12Q2537/143 , C12Q2537/125 , C12Q2525/313
Abstract: Methods of detecting one or more nucleic acids from whole blood or plasma are provided. The nucleic acids are captured on a solid support and detected. Compositions, kits, and systems related to the methods are also described.
Abstract translation: 提供从全血或血浆中检测一种或多种核酸的方法。 将核酸捕获在固体支持物上并检测。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US20070015188A1
公开(公告)日：2007-01-18
申请号：US11471025
申请日：2006-06-19
Applicant: Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
Inventor： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
IPC: C12Q1/68
CPC classification number: C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
Abstract: Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.
Abstract translation: 提供了检测核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测到在固体支持物上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US20060286583A1
公开(公告)日：2006-12-21
申请号：US11433081
申请日：2006-05-11
Applicant: Yuling Luo , Wen Yang
Inventor： Yuling Luo , Wen Yang
CPC classification number: C12Q1/6837 , C12Q1/682 , C12Q1/6834 , C12Q2537/143 , C12Q2537/125 , C12Q2525/313 , C12Q2565/543 , C12Q2565/501 , C12Q2563/149 , C12Q2563/103
Abstract: Methods of detecting two or more nucleic acids in a multiplex branched-chain DNA assay are provided. Different nucleic acids are captured through cooperative hybridization events on different, identifiable subsets of particles or at different selected positions on a spatially addressable solid support. Compositions, kits, and systems related to the methods are also described.
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