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1.发明申请Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations 审中-公开检测单个细胞中的核酸并鉴定来自大异质细胞群体的稀有细胞的方法公开(公告)号：US20090081688A1
公开(公告)日：2009-03-26
申请号：US12284163
申请日：2008-09-17
Applicant: Yuling Luo , Shiping Chen , Huei-Yu Fay Wang , John James Flanagan
Inventor： Yuling Luo , Shiping Chen , Huei-Yu Fay Wang , John James Flanagan
IPC: C12Q1/68
CPC classification number: C12Q1/6841 , C12Q2537/143 , C12Q2537/125
Abstract: Methods of detecting multiple nucleic acid targets in single cells through indirect capture of labels to the nucleic acids are provided. Methods of assaying the relative levels of nucleic acid targets through normalization to levels of reference nucleic acids are also provided. Methods of detecting individual cells, particularly rare cells from large heterogeneous cell populations, through detection of nucleic acids are described. Related compositions, systems, and kits are also provided.
Abstract translation: 提供了通过间接捕获标记到核酸来检测单个细胞中的多个核酸靶的方法。 还提供了通过归一化到参考核酸水平来测定核酸靶的相对水平的方法。 描述了通过检测核酸检测单个细胞，特别是来自大异质细胞群体的罕见细胞的方法。 还提供了相关的组合物，系统和试剂盒。
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公开(公告)号：US06594432B2
公开(公告)日：2003-07-15
申请号：US09791994
申请日：2001-02-22
Applicant: Shiping Chen , Yuling Luo
Inventor： Shiping Chen , Yuling Luo
IPC: G02B600
CPC classification number: G01N21/6458 , B01J19/0046 , B01J2219/00369 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00628 , B01J2219/0063 , B01J2219/00637 , B01J2219/00659 , B01J2219/00673 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/0262 , B01L3/0268 , B01L3/563 , B01L2200/021 , B01L2300/0819 , B01L2300/0829 , B01L2400/022 , B01L2400/027 , C40B40/06 , C40B40/10 , C40B60/14 , G01N21/6452 , G01N35/1074 , G01N2021/6484 , G01N2035/1037
Abstract: Disclosed is a microarray printing system and methods of printing probe microarrays. The system has a print head formed of one or more bundles of individual capillaries, such as light-guiding capillaries. The bundles may especially be random bundles of capillaries that provide a large number of probes on the surface of a substrate. Methods of registering or correlating the distal and proximal ends of the capillaries are also provided. Further, the invention provides methods and equipment for identifying defective microarrays that are missing one or more probes from the surface of the microarray.
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3.发明申请Methods of detecting nucleic acids in individual cells and of identifying rare cells from large heterogeneous cell populations 审中-公开检测单个细胞中的核酸并鉴定来自大异质细胞群体的稀有细胞的方法公开(公告)号：US20080038725A1
公开(公告)日：2008-02-14
申请号：US11471278
申请日：2006-06-19
Applicant: Yuling Luo , Shiping Chen
Inventor： Yuling Luo , Shiping Chen
IPC: C12Q1/68
CPC classification number: C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
Abstract: Methods of detecting multiple nucleic acid targets in single cells through indirect capture of labels to the nucleic acids are provided. Methods of assaying the relative levels of nucleic acid targets through normalization to levels of reference nucleic acids are also provided. Methods of detecting individual cells, particularly rare cells from large heterogeneous cell populations, through detection of nucleic acids are described. Related compositions, systems, and kits are also provided.
Abstract translation: 提供了通过间接捕获标记到核酸来检测单个细胞中的多个核酸靶的方法。 还提供了通过归一化到参考核酸水平来测定核酸靶的相对水平的方法。 描述了通过检测核酸检测单个细胞，特别是来自大异质细胞群体的罕见细胞的方法。 还提供了相关的组合物，系统和试剂盒。
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公开(公告)号：US06953551B2
公开(公告)日：2005-10-11
申请号：US09791998
申请日：2001-02-22
Applicant: Shiping Chen , Yuling Luo
Inventor： Shiping Chen , Yuling Luo
IPC: G01N33/53 , B01J4/00 , B01J19/00 , B01L3/02 , B81B1/00 , C12M1/00 , C12N15/09 , C40B40/06 , C40B40/10 , C40B60/14 , G01N21/25 , G01N21/64 , G01N35/10 , G01N37/00 , B41K1/38
CPC classification number: B01L3/0268 , B01J19/0046 , B01J2219/00369 , B01J2219/00382 , B01J2219/00385 , B01J2219/00418 , B01J2219/00527 , B01J2219/00529 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/0061 , B01J2219/00612 , B01J2219/00619 , B01J2219/00621 , B01J2219/00626 , B01J2219/00637 , B01J2219/00648 , B01J2219/00659 , B01J2219/00707 , B01J2219/00722 , B01J2219/00725 , B01L3/0262 , C40B40/06 , C40B40/10 , C40B60/14 , G01N21/253 , G01N21/6452 , G01N35/1074 , G01N2021/6484 , G01N2035/1037
Abstract: Disclosed is a microarray printing system and methods of printing probe microarrays. The system has a print-head formed of one or more capillary bundle, such as light-guiding capillaries. The bundles may especially be bundles of capillaries that provide a large number of probes on the surface of a substrate. Methods of registering or correlating the distal and proximal ends of the capillaries are also provided. Further, the invention provides methods and equipment for identifying defective microarrays that are missing one or more probes from the surface of the microarray.
Abstract translation: 公开了一种微阵列打印系统和打印探针微阵列的方法。 该系统具有由一个或多个毛细管束形成的打印头，例如导光毛细管。 束可以特别地是在基底表面上提供大量探针的毛细管束。 也提供了注射或关联毛细管的远端和近端的方法。 此外，本发明提供了用于鉴定从微阵列表面缺少一个或多个探针的有缺陷的微阵列的方法和设备。
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公开(公告)号：US20130171621A1
公开(公告)日：2013-07-04
申请号：US13575936
申请日：2011-01-31
Applicant: Yuling Luo , Huei-Yu Wang , John James Flanagan , Shiping Chen
Inventor： Yuling Luo , Huei-Yu Wang , John James Flanagan , Shiping Chen
IPC: C12Q1/68
CPC classification number: C12Q1/6881 , C12Q1/6841 , C12Q2600/158 , C12Q2565/626 , C12Q2545/114
Abstract: Methods of detecting the presence or absence of a class of nucleic acid targets in single cells through direct or indirect capture of labels to the nucleic acids are provided, where such labels to the class of nucleic acid targets are indistinguishable from each other. Also described are methods of detecting individual cells, particularly a cell of a specific type from large heterogeneous cell populations, through detection of one or more of nucleic acid targets, where the labels to the one or more of nucleic acid targets are indistinguishable from each other. Related kits are also described.
Abstract translation: 提供了通过对核酸直接或间接捕获标记来检测单细胞中一类核酸靶的存在或不存在的方法，其中与核酸靶标类别的这种标记彼此不可区分。 还描述了通过检测一个或多个核酸靶标来检测单个细胞，特别是来自大异质细胞群体的特定类型的细胞的方法，其中一个或多个核酸靶标的标记彼此不可区分 。 还描述了相关工具包。
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Patent Agency Ranking
公开(公告)号：US08951726B2
公开(公告)日：2015-02-10
申请号：US12660524
申请日：2010-02-26
Applicant: Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
Inventor： Yuling Luo , Yunqing Ma , Cung-Tuong Nguyen
CPC classification number: C12Q1/6837 , C12Q1/682 , C12Q1/6841 , C12Q2537/143 , C12Q2537/125 , C12Q2565/543 , C12Q2525/313
Abstract: Methods of detecting nucleic acids, including methods of detecting two or more nucleic acids in multiplex branched-chain DNA assays, are provided. Nucleic acids captured on a solid support are detected, for example, through cooperative hybridization events that result in specific association of a label with the nucleic acids. Compositions, kits, and systems related to the methods are also described.
Abstract translation: 提供了检测核酸的方法，包括在多重支链DNA测定中检测两种或多种核酸的方法。 检测到在固体支持物上捕获的核酸，例如通过导致标记与核酸的特异性缔合的协同杂交事件。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US20110105351A1
公开(公告)日：2011-05-05
申请号：US12804904
申请日：2010-07-29
Applicant: Yuling Luo , Wen Yang
Inventor： Yuling Luo , Wen Yang
CPC classification number: C12Q1/6837 , C12Q1/682 , C12Q1/6834 , C12Q2537/143 , C12Q2537/125 , C12Q2525/313 , C12Q2565/543 , C12Q2565/501 , C12Q2563/149 , C12Q2563/103
Abstract: Methods of detecting two or more nucleic acids in a multiplex branched-chain DNA assay are provided. Different nucleic acids are captured through cooperative hybridization events on different, identifiable subsets of particles or at different selected positions on a spatially addressable solid support. Compositions, kits, and systems related to the methods are also described.
Abstract translation: 提供了在多重支链DNA测定中检测两种或多种核酸的方法。 通过在不同的，可识别的颗粒子集上或在空间可寻址的固体支持物上的不同选择位置上的协同杂交事件捕获不同的核酸。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US07803541B2
公开(公告)日：2010-09-28
申请号：US11433081
申请日：2006-05-11
Applicant: Yuling Luo , Wen Yang
Inventor： Yuling Luo , Wen Yang
CPC classification number: C12Q1/6837 , C12Q1/682 , C12Q1/6834 , C12Q2537/143 , C12Q2537/125 , C12Q2525/313 , C12Q2565/543 , C12Q2565/501 , C12Q2563/149 , C12Q2563/103
Abstract: Methods of detecting two or more nucleic acids in a multiplex branched-chain DNA assay are provided. Different nucleic acids are captured through cooperative hybridization events on different, identifiable subsets of particles or at different selected positions on a spatially addressable solid support. Compositions, kits, and systems related to the methods are also described.
Abstract translation: 提供了在多重支链DNA测定中检测两种或多种核酸的方法。 通过在不同的，可识别的颗粒子集上或在空间可寻址的固体支持物上的不同选择位置上的协同杂交事件捕获不同的核酸。 还描述了与方法相关的组合物，试剂盒和系统。
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公开(公告)号：US08632970B2
公开(公告)日：2014-01-21
申请号：US12930041
申请日：2010-12-23
Applicant: Yuling Luo , Wen Yang
Inventor： Yuling Luo , Wen Yang
CPC classification number: C12Q1/6874 , C12Q1/6832 , C12Q1/6834 , C12Q1/6837 , C12Q2537/143 , C12Q2563/143 , C12Q2563/149
Abstract: Methods of capturing two or more nucleic acids simultaneously from a single sample are provided. Different nucleic acids are captured through cooperative hybridization events on different subsets of particles or at different selected positions on a spatially addressable solid support. Methods of capturing one or more long nucleic acids and methods of capturing one or more nucleic acid for sequencing are also provided. Compositions, kits, and systems related to the methods are also described.
Abstract translation: 提供了从单个样品同时捕获两种或更多种核酸的方法。 通过在不同的颗粒子集上或在空间上可寻址的固体支持物上的不同选择位置上的协同杂交事件捕获不同的核酸。 还提供了捕获一种或多种长核酸的方法和捕获一种或多种核酸进行测序的方法。 还描述了与方法相关的组合物，试剂盒和系统。
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10.发明申请RNASCOPE® HPV ASSAY FOR DETERMINING HPV STATUS IN HEAD AND NECK CANCERS AND CERVICAL LESIONS 审中-公开用于确定头颈部和颈部癌症和宫颈癌HPV状态的RNASCOPE®HPV测定公开(公告)号：US20120214152A1
公开(公告)日：2012-08-23
申请号：US13359706
申请日：2012-01-27
Applicant: Xiao-Jun Ma , John Flanagan , Yuling Luo
Inventor： Xiao-Jun Ma , John Flanagan , Yuling Luo
CPC classification number: C12Q1/708 , C12Q1/6841 , C12Q1/6886 , C12Q2600/112 , C12Q2600/118
Abstract: The present invention provides a method and a kit for determining whether a head and neck cancer is HPV-related. In one embodiment, an RNAscope® HPV assay was designed to detect the presence of E6/E7 mRNA of certain high-risk HPV subtypes related to head and neck cancer. The present invention also provides a method and a kit for determining whether a cervical lesion is a benign lesion or a cervical intraepithethial neoplasm lesion. The present invention further provides a method for determining the progression of cervical intraepithethial neoplasm based on the spatial pattern and levels of the E6/E7 mRNA of certain high-risk HPV subtypes. The present invention also provides a method for determining the risk of developing cervical cancer in a human diagnosed with cervical intraepithethial neoplasm based on presence and absence of the certain subgroups of high-risk HPV subtypes.
Abstract translation: 本发明提供了一种用于确定头颈癌是否与HPV相关的方法和试剂盒。 在一个实施方案中，设计了RNA检测HPV检测来检测某些与头颈癌相关的高危HPV亚型的E6 / E7 mRNA的存在。 本发明还提供了一种用于确定子宫颈病变是否是良性病变或子宫颈上皮内肿瘤病变的方法和试剂盒。 本发明还提供了一种基于某些高风险HPV亚型的E6 / E7 mRNA的空间格局和水平来确定子宫颈上皮内肿瘤进展的方法。 本发明还提供了一种基于存在和不存在某些亚型的高风险HPV亚型的方法来确定诊断为宫颈上皮内瘤的人的宫颈癌发生风险的方法。
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