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    Process for purifying cyclolipopeptide compounds or the salts thereof
    14.
    发明授权
    Process for purifying cyclolipopeptide compounds or the salts thereof 有权
    环肽肽化合物或其盐的纯化方法

    公开(公告)号：US08877777B2

    公开(公告)日：2014-11-04

    申请号：US13877141

    申请日：2011-09-27

    申请人： Zhaoli Zhang ,  Shidong Liu ,  Zhonghao Zhuo ,  Xiaoming Ji

    发明人： Zhaoli Zhang ,  Shidong Liu ,  Zhonghao Zhuo ,  Xiaoming Ji

    IPC分类号： A61K38/12 ,  C07K5/00 ,  C07K7/00 ,  C07K16/00 ,  C07K17/00 ,  C07K7/56

    CPC分类号： C07K7/56

    摘要： A method for purifying a cyclic lipopeptide or a salt thereof is provided. The method comprises the steps: (1) extracting a fermentation broth containing a compound of formula I or a salt thereof, to obtain an extract 1 after filtration or centrifugation; (2) diluting or concentrating the extract 1 under vacuum to decrease the content of the organic solvent, to obtain an extract 2; (3) loading the extract 2 onto a macroporous absorption resin; (4) washing the macroporous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution; and (5) eluting the compound of formula I off from the macroporous adsorption resin with water, an organic solvent, or a mixture of water and an organic solvent as a washing solution as an eluant. Compared with the prior art, the purification method has the advantages that fewer organic solvent is used, no silica gel is used, the harm to the environment is less, and the purity of the collected compound of formula I is improved.

    摘要翻译： 提供了一种纯化环状肽或其盐的方法。 该方法包括以下步骤：（1）提取含有式I化合物或其盐的发酵液，过滤或离心后得到提取物1; （2）真空稀释或浓缩提取物1以降低有机溶剂的含量，得到提取物2; （3）将提取物2装载到大孔吸附树脂上; （4）用水，有机溶剂或水和有机溶剂的混合物洗涤大孔吸附树脂作为洗涤液; 和（5）用水，有机溶剂或水和有机溶剂的混合物作为洗脱液从大孔吸附树脂中除去式I化合物作为洗脱剂。 与现有技术相比，纯化方法的优点是使用少量有机溶剂，不使用硅胶，对环境的危害较小，提取了所收集的式I化合物的纯度。