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    Mutagenized strain of Glarea lozoyensis and a method of preparing a compound from the mutagenized strain
    3.
    发明授权
    Mutagenized strain of Glarea lozoyensis and a method of preparing a compound from the mutagenized strain 失效
    变形菌株Glarea lozoyensis和从诱变菌株制备化合物的方法

    公开(公告)号：US08431383B2

    公开(公告)日：2013-04-30

    申请号：US13497927

    申请日：2009-09-28

    申请人： Jing Xu ,  Yi Chen ,  Xiaoming Ji ,  Xiaoliang Gao ,  Shidong Liu ,  Zhaoli Zhang

    发明人： Jing Xu ,  Yi Chen ,  Xiaoming Ji ,  Xiaoliang Gao ,  Shidong Liu ,  Zhaoli Zhang

    IPC分类号： C12N1/20 ,  C07K7/56

    CPC分类号： C12R1/645 ,  C07K7/56 ,  C12N15/01

    摘要： High yield antibiotics producing fungus strain, preparation method and use thereof are provided. The fungus strain is a mutant derived from Glarea lozoyensis, and deposited in CGMCC with the accession number of CGMCC 2933. The preparation method concludes following steps: (a) mixing the culture media of Glarea lozoyensis strain ATCC 20957 with nitrosoguanidine, and obtaining mixture a; (b) mixing lywallzyme with the mixture a, and obtaining protoplasts; (c) regenerating the protoplasts, and obtaining single clones; and (d) culturing the single clones, then obtaining the mutant strain. This fungus strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable to be used in industry.

    摘要翻译： 提供生产真菌菌株的高产抗生素，其制备方法和用途。 真菌菌株是来自山茱萸的突变体，保藏于CGMCC，保藏号为CGMCC 2933.制备方法的结论如下：（a）将葛兰氏杆菌菌株ATCC 20957的培养基与亚硝基胍混合，得到混合物 ; （b）将溶菌酶与混合物a混合，得到原生质体; （c）再生原生质体，获得单个克隆; 和（d）培养单个克隆，然后获得突变株。 该真菌菌株具有稳定的遗传和生产性能，在发酵中产生的杂质少，适用于工业。

    HIGH YIELD ANTIBIOTICS PRODUCING FUNGUS STRAIN, PREPARATION METHOD AND USE THEREOF
    4.
    发明申请
    HIGH YIELD ANTIBIOTICS PRODUCING FUNGUS STRAIN, PREPARATION METHOD AND USE THEREOF 失效
    生产真菌菌株的高效抗生素，制备方法及其应用

    公开(公告)号：US20120258498A1

    公开(公告)日：2012-10-11

    申请号：US13497927

    申请日：2009-09-28

    申请人： Jing Xu ,  Yi Chen ,  Xiaoming Ji ,  Xiaoliang Gao ,  Shidong Liu ,  Zhaoli Zhang

    发明人： Jing Xu ,  Yi Chen ,  Xiaoming Ji ,  Xiaoliang Gao ,  Shidong Liu ,  Zhaoli Zhang

    IPC分类号： A01H15/00 ,  C12P21/04

    CPC分类号： C12R1/645 ,  C07K7/56 ,  C12N15/01

    摘要： High yield antibiotics producing fungus strain, preparation method and use thereof are provided. The fungus strain is a mutant derived from Glarea lozoyensis, and deposited in CGMCC with the accession number of CGMCC 2933. The preparation method concludes following steps: (a) mixing the culture media of Glarea lozoyensis strain ATCC 20957 with nitrosoguanidine, and obtaining mixture a; (b) mixing lywallzyme with the mixture a, and obtaining protoplasts; (c) regenerating the protoplasts, and obtaining single clones; and (d) culturing the single clones, then obtaining the mutant strain. This fungus strain has stable genetic and producing property, produces little impurities in fermentation, and is suitable to be used in industry.

    摘要翻译： 提供生产真菌菌株的高产抗生素，其制备方法和用途。 真菌菌株是来自山茱萸的突变体，保藏于CGMCC，保藏号为CGMCC 2933.制备方法的结论如下：（a）将葛兰氏杆菌菌株ATCC 20957的培养基与亚硝基胍混合，得到混合物 ; （b）将溶菌酶与混合物a混合，得到原生质体; （c）再生原生质体，获得单个克隆; 和（d）培养单个克隆，然后获得突变株。 该真菌菌株具有稳定的遗传和生产性能，在发酵中产生的杂质少，适用于工业。