公开(公告)号：US20060083746A1

公开(公告)日：2006-04-20

申请号：US11047224

申请日：2005-01-31

申请人： John Young ,  Kenneth Bradley ,  R. Collier ,  Jeremy Mogridge

发明人： John Young ,  Kenneth Bradley ,  R. Collier ,  Jeremy Mogridge

IPC分类号： A61K39/40 ,  C07H21/04 ,  C12N15/74 ,  C12N1/21 ,  C07K14/32

CPC分类号： C07K14/705 ,  A61K38/00

摘要： The present invention relates to mammalian anthrax toxin receptor polypeptides and polynucleotides encoding same as well as related polypeptides and polynucleotides, vectors containing the polynucleotides and polypeptides, host cells containing related polynucleotide molecules, and cells displaying no anthrax toxin receptor on an exterior surface of the cells-minus cell lines and animals. The present invention also relates to methods for identifying molecules that bind the anthrax toxin receptor and molecules that reduce the toxicity of anthrax toxin. Finally, the present invention provides methods for treating human and non-human animals suffering from anthrax.

摘要翻译： 本发明涉及哺乳动物炭疽毒素受体多肽和编码其的多核苷酸以及相关多肽和多核苷酸，含有多核苷酸和多肽的载体，含有相关多核苷酸分子的宿主细胞和在细胞外表面上不显示炭疽毒素受体的细胞 - 细胞系和动物。 本发明还涉及用于鉴定结合炭疽毒素受体的分子和降低炭疽毒素毒性的分子的方法。 最后，本发明提供了治疗患有炭疽的人类和非人类动物的方法。

公开(公告)号：US20050247866A1

公开(公告)日：2005-11-10

申请号：US11079533

申请日：2005-03-15

申请人： Joseph Plewa ,  Evan Tanner ,  Daniel Mueth ,  Lewis Gruber ,  Kenneth Bradley

发明人： Joseph Plewa ,  Evan Tanner ,  Daniel Mueth ,  Lewis Gruber ,  Kenneth Bradley

IPC分类号： G21K1/00 ,  H05H3/04

CPC分类号： B82Y20/00 ,  B82Y10/00 ,  G03H1/08 ,  G03H1/2294 ,  G03H2001/0077 ,  G03H2225/32

摘要： A method an apparatus for manipulating particles (micro, nano, and pico) having one or more characteristics with an optical trap formed by modulating a laser beam with a Diffractive Optical Element (DOE). At least one characteristic of the material is selected; and a laser beam having a selected wavelength corresponding to the at least one selected characteristic of the material is generated. Values of the DOE are calculated corresponding to the at least one selected characteristic of the material. The beam and the DOE are modulated to produce a holographic optical trap having properties corresponding to the at least one selected characteristic; the trap is focused to a beam focus or selected spot size; and the beam focus is located near a particle location for trapping the particle therein.

摘要翻译： 一种用于通过用衍射光学元件（DOE）调制激光束形成的光阱来操纵具有一个或多个特性的微粒（微，纳米和微微）的装置的方法。 选择材料的至少一种特性; 并且产生具有与材料的至少一个所选特征对应的选定波长的激光束。 根据材料的至少一个所选特征计算DOE的值。 光束和DOE被调制以产生具有对应于至少一个所选特征的性质的全息光阱; 陷阱聚焦到光束焦点或选定的光斑尺寸; 并且束聚焦位于颗粒位置附近以将颗粒捕获在其中。

公开(公告)号：US06718083B2

公开(公告)日：2004-04-06

申请号：US10394172

申请日：2003-03-24

申请人： Ward Lopes ,  Kenneth Bradley

发明人： Ward Lopes ,  Kenneth Bradley

IPC分类号： G02B626

CPC分类号： G02B6/29341 ,  G02B6/29352 ,  G02F1/3132 ,  G02F2203/15 ,  G02F2203/585

摘要： An optical switch and optical router to rapidly (in the range of nanoseconds to picoseconds) route signals from particular channels within an optical band by using optical switches which utilize a controlled whispering gallery mode (WGM) resonance of dielectric microspheres to optically switch signal by virtue of their evanescent waves.

摘要翻译： 光学开关和光路由器快速（在纳秒到皮秒范围内）通过使用光电开关对来自光频带内的特定通道的信号进行信号传递，该光开关利用电介质微球的受控耳语画廊模式（WGM）共振以通过美学光学切换信号 的消逝波。

公开(公告)号：US08169600B2

公开(公告)日：2012-05-01

申请号：US12440949

申请日：2007-09-14

申请人： Christopher Knutson ,  Crystal Duke ,  Gary Stacey ,  Dan Mueth ,  Evan Tanner ,  Osman Akcakir ,  Haojun Fu ,  Robert Lancelot ,  Tania Chakrabarty ,  Kenneth Bradley

发明人： Christopher Knutson ,  Crystal Duke ,  Gary Stacey ,  Dan Mueth ,  Evan Tanner ,  Osman Akcakir ,  Haojun Fu ,  Robert Lancelot ,  Tania Chakrabarty ,  Kenneth Bradley

IPC分类号： G01N33/48

CPC分类号： G01N33/48 ,  G01N33/54373 ,  G01N33/80

摘要： Methods and apparatus for analyzing surface properties of particles are provided. A method for analyzing the surface properties of the particle includes a associating a first particle with a first capture zone having a specific binding affinity for a first chemical species, applying an optical force to the first particle, sensing a response of the first particle to the optical force, and using the sensed response to determine the presence, absence or quantity of the first chemical species on the first particle surface. This process may be repeated in parallel to test multiple particles. In addition to directly testing the surface properties of the particles, the method can be used in direct, indirect and competitive assays to determine the presence, absence or quantity of free or immobilized analytes. A fluidic cartridge with capture zones having avidities that are tuned for the use of optical forces is provided. A software routine for performing the method is also provided.

摘要翻译： 提供了分析颗粒表面性质的方法和装置。 用于分析颗粒的表面性质的方法包括将第一颗粒与对第一化学物质具有特异性结合亲和力的第一捕获区缔合，向第一颗粒施加光学力，感测第一颗粒对第一颗粒的响应 并且使用感测到的响应来确定第一颗粒表面上的第一化学物质的存在，不存在或数量。 该过程可以并行重复以测试多个颗粒。 除了直接测试颗粒的表面性质之外，该方法可用于直接，间接和竞争性测定，以确定游离或固定化分析物的存在，不存在或数量。 提供了一种具有捕获区域的流体盒，其具有被调整以用于光学力的空间。 还提供了用于执行该方法的软件程序。

公开(公告)号：US20090108190A1

公开(公告)日：2009-04-30

申请号：US12230304

申请日：2008-08-27

申请人： Joseph Plewa ,  Evan Tanner ,  Dan Mueth ,  Lewis Gruber ,  Kenneth Bradley

发明人： Joseph Plewa ,  Evan Tanner ,  Dan Mueth ,  Lewis Gruber ,  Kenneth Bradley

IPC分类号： H05H3/04

CPC分类号： B82Y10/00 ,  G03H1/08 ,  G03H1/2294 ,  G03H2001/0077 ,  G03H2225/32

摘要： A method for manipulating particles (micro, nano, and pico) having one or more characteristics with an optical trap formed by modulating a laser beam with a Diffractive Optical Element (DOE). At least one characteristic of the material is selected; and a laser beam having a selected wavelength corresponding to the at least one selected characteristic of the material is generated. Values of the DOE are calculated corresponding to the at least one selected characteristic of the material. The beam and the DOE are modulated to produce a holographic optical trap having properties corresponding to the at least one selected characteristic; the trap is focused to a beam focus or selected spot size; and the beam focus is located near a particle location for trapping the particle therein.

摘要翻译： 一种通过用衍射光学元件（DOE）调制激光束形成的光阱来操纵具有一个或多个特性的微粒（微，纳米和微微）的方法。 选择材料的至少一种特性; 并且产生具有与材料的至少一个所选特征对应的选定波长的激光束。 根据材料的至少一个所选特征计算DOE的值。 光束和DOE被调制以产生具有对应于至少一个所选特征的性质的全息光阱; 陷阱聚焦到光束焦点或选定的光斑尺寸; 并且束聚焦位于颗粒位置附近以将颗粒捕获在其中。

公开(公告)号：US07411181B2

公开(公告)日：2008-08-12

申请号：US10974976

申请日：2004-10-28

申请人： Joseph Plewa ,  Evan Tanner ,  Dan Mueth ,  Lewis Gruber ,  Kenneth Bradley

发明人： Joseph Plewa ,  Evan Tanner ,  Dan Mueth ,  Lewis Gruber ,  Kenneth Bradley

IPC分类号： G02B5/18 ,  G02B15/32

CPC分类号： G03H1/22 ,  G02B5/32 ,  G03H1/0005 ,  G03H1/08 ,  G03H1/2294 ,  G03H2001/0077 ,  G03H2001/2284

摘要： A method and apparatus for manipulating particles (micro, nano, and pico) having one or more characteristics with an optical trap formed by modulating a laser beam with a Diffractive Optical Element (DOE). At least one characteristic of the material is selected; and a laser beam having a selected wavelength corresponding to the at least one selected characteristic of the material is generated. Values of the DOE are calculated corresponding to the at least one selected characteristic of the material. The beam and the DOE are modulated to produce a holographic optical trap having properties corresponding to the at least one selected characteristic. The trap is focused to a beam focus or selected spot size; and the beam focus is located near a particle location for trapping the particle therein.

摘要翻译： 一种用于通过用衍射光学元件（DOE）调制激光束而形成的光阱来操纵具有一个或多个特性的微粒（微，纳米和微微）的方法和装置。 选择材料的至少一种特性; 并且产生具有与材料的至少一个所选特征对应的选定波长的激光束。 根据材料的至少一个所选特征计算DOE的值。 束和DOE被调制以产生具有对应于至少一个所选特征的性质的全息光阱。 陷阱聚焦到光束对焦或选定的光斑尺寸; 并且束聚焦位于颗粒位置附近以将颗粒捕获在其中。

公开(公告)号：US20070172500A1

公开(公告)日：2007-07-26

申请号：US11340424

申请日：2006-01-26

申请人： John Young ,  Heather Scobie ,  G. Jonah Rainey ,  Kenneth Bradley

发明人： John Young ,  Heather Scobie ,  G. Jonah Rainey ,  Kenneth Bradley

IPC分类号： A61K39/07 ,  C07H21/04 ,  C07K14/32 ,  C12N15/74 ,  C12N1/21

CPC分类号： C07K16/1278 ,  A61K38/00 ,  C07K14/705 ,  C07K14/78

摘要： The present invention provides compositions useful in preparing and/or serving as antitoxins against Bacillus anthracis, the causative agent of anthrax. The present invention also provides polypeptides and polynucleotides relating to the capillary morphogenisis gene 2 (CMG-2), vectors containing the polynucleotides and polypeptides, and host cells containing related polynucleotide molecules, all used in association with the treatment of, or the research and development of treatments for anthrax. The present invention also relates to methods for identifying molecules that bind CMG-2 and molecules that reduce the toxicity of anthrax toxin. Finally, the present invention provides methods for treating human and non-human animals suffering from anthrax.

摘要翻译： 本发明提供了可用于制备和/或用作抗炭疽杆菌（Anthracis）的抗毒素的组合物，炭疽芽孢杆菌的致病因子。 本发明还提供了与毛细血管形成形成发生基因2（CMG-2）相关的多肽和多核苷酸，含有多核苷酸和多肽的载体，以及包含相关多核苷酸分子的宿主细胞，所有这些都与治疗或研究和开发相结合 的炭疽治疗。 本发明还涉及用于鉴定结合CMG-2的分子和降低炭疽毒素毒性的分子的方法。 最后，本发明提供了治疗患有炭疽的人类和非人类动物的方法。

公开(公告)号：US07241988B2

公开(公告)日：2007-07-10

申请号：US10630904

申请日：2003-07-31

申请人： Lewis Gruber ,  Kenneth Bradley ,  Ward Lopes ,  Robert W. Lancelot ,  Joseph S. Plewa ,  David G. Grier

发明人： Lewis Gruber ,  Kenneth Bradley ,  Ward Lopes ,  Robert W. Lancelot ,  Joseph S. Plewa ,  David G. Grier

IPC分类号： G01N21/63

CPC分类号： G01N15/1459 ,  B01L3/5027 ,  G01N2015/149 ,  G02B21/32 ,  G03H1/08 ,  G03H1/2294 ,  G03H2001/0077 ,  G03H2001/085 ,  G03H2225/32 ,  H05H3/04

摘要： The present invention employs a beam steering apparatus to isolate valuable cells from other cells, tissues, and contaminants. In one embodiment, the system balances optical trapping against biasing flow to parallelize cell sorting under the flexible control of computer program-directed traps which differentially manipulate cells based on their composition or labels to direct separation.

摘要翻译： 本发明采用光束转向装置来将有价值的细胞与其它细胞，组织和污染物分离。 在一个实施例中，系统平衡光学捕获与偏置流平衡，以在计算机程序导向的陷阱的灵活控制下平行化细胞分选，其基于它们的组成或标签差异地操纵细胞以引导分离。

公开(公告)号：US20070117159A1

公开(公告)日：2007-05-24

申请号：US11483872

申请日：2006-07-10

申请人： John Young ,  Kenneth Bradley ,  R. Collier ,  Jeremy Mogridge

发明人： John Young ,  Kenneth Bradley ,  R. Collier ,  Jeremy Mogridge

IPC分类号： G01N33/567 ,  C07H21/04 ,  C12P21/06 ,  C12N1/21 ,  C07K14/74 ,  C07K14/32 ,  C12N15/74

CPC分类号： C07K14/705 ,  A61K38/00

摘要： The present invention relates to mammalian anthrax toxin receptor polypeptides and polynucleotides encoding same as well as related polypeptides and polynucleotides, vectors containing the polynucleotides and polypeptides, host cells containing related polynucleotide molecules, and cells displaying no anthrax toxin receptor on an exterior surface of the cells-minus cell lines and animals. The present invention also relates to methods for identifying molecules that bind the anthrax toxin receptor and molecules that reduce the toxicity of anthrax toxin. Finally, the present invention provides methods for treating human and non-human animals suffering from anthrax.

摘要翻译： 本发明涉及哺乳动物炭疽毒素受体多肽和编码其的多核苷酸以及相关多肽和多核苷酸，含有多核苷酸和多肽的载体，含有相关多核苷酸分子的宿主细胞和在细胞外表面上不显示炭疽毒素受体的细胞 - 细胞系和动物。 本发明还涉及用于鉴定结合炭疽毒素受体的分子和降低炭疽毒素毒性的分子的方法。 最后，本发明提供了治疗患有炭疽的人类和非人类动物的方法。

公开(公告)号：US20060130859A1

公开(公告)日：2006-06-22

申请号：US10527286

申请日：2003-08-28

申请人： John-Paul Mua ,  Brad Hayes ,  Kenneth Bradley

发明人： John-Paul Mua ,  Brad Hayes ,  Kenneth Bradley

IPC分类号： A24B15/24

CPC分类号： A24B15/243 ,  A24B15/24 ,  A24B15/245

摘要： A process for reducing lignin and nitrogenous content in tobacco lamina and tobacco fiber material, including whole leaf, stems, scraps, fines and lamina, as well as burley leaf and stem, in an extraction with a solution containing hydrogen peroxide and an alkali metal hydroxide. The treated tobacco may then be further processed for use in cigarettes and other smoking articles.

摘要翻译： 一种用含有过氧化氢和碱金属氢氧化物的溶液萃取的方法，用于降低烟草层和烟草纤维材料中的木质素和含氮量，包括全叶，茎，碎屑，细粉和薄片以及白肋叶和茎 。 然后处理的烟草可以进一步处理以用于香烟和其他吸烟制品。