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公开(公告)号：US09850506B2
公开(公告)日：2017-12-26
申请号：US15116896
申请日：2015-05-02
Applicant: BASF SE
Inventor： Joanna Martyna Krawczyk , Stefan Haefner , Hartwig Schröder , Esther Dantas Costa , Oskar Zelder , Gregory Von Abendroth
IPC: C12P7/46
CPC classification number: C12P7/46 , C07K14/285 , C12N1/20 , C12N15/52
Abstract: The present invention relates to a modified microorganism having, compared to its wild-type, a reduced activity of the enzyme that is encoded by the fruA-gene. The present invention also relates to a method for producing an organic compound and to the use of modified microorganisms.
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12.发明申请GENETICALLY MODIFIED MICROORGANISMS CAPABLE OF PRODUCING BETA-GLUCANS AND METHODS FOR PRODUCING BETA-GLUCANS 有权能够生产β-葡聚糖的基因改造的微生物和用于生产β-葡聚糖的方法公开(公告)号：US20160194676A1
公开(公告)日：2016-07-07
申请号：US15074330
申请日：2016-03-18
Applicant: BASF SE , Wintershall Holding GmbH
Inventor： Beata Brockmann , Andrea Herold , Oskar Zelder , Stefan Haefner , Christian Fleck , Hartwig Schröder , Mari Granström , Julia Kristiane Schmidt
CPC classification number: C12P19/04 , C08B37/0024 , C08L5/00 , C12N9/1051 , C12N15/80 , C12P19/18 , C12Y204/01034 , Y02P20/52
Abstract: The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that the genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-β-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity or the use of such a polypeptide for producing β-glucans. Furthermore, the present invention relates to methods for producing β-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity thereby increasing the expression of the polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity into a microorganism being able to synthesize β-glucans.
Abstract translation: 本发明涉及能够产生β-葡聚糖的转基因微生物，其特征在于，遗传修饰的微生物过表达（i）编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸和/或（ ii）与相同菌株的相应未修饰的对照微生物相比，具有1,3-葡聚糖 - D-葡聚糖合酶活性的多肽。 本发明还涉及编码具有1,3-葡萄糖 - D-葡聚糖合酶活性的多肽的多核苷酸的用途或这种多肽用于生产葡聚糖的用途。 此外，本发明涉及生产葡聚糖的方法，包括在编码具有1,3-葡萄糖-D-葡聚糖合酶活性的多肽的多核苷酸上游引入启动子，从而增加多核苷酸的表达，或 编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸能够合成葡聚糖。
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13.发明申请GENETICALLY MODIFIED MICROORGANISMS CAPABLE OF PRODUCING BETA-GLUCANS AND METHODS FOR PRODUCING BETA-GLUCANS 审中-公开能够生产β-葡聚糖的基因改造的微生物和用于生产β-葡聚糖的方法公开(公告)号：US20150152453A1
公开(公告)日：2015-06-04
申请号：US14412212
申请日：2013-07-03
Applicant: BASF SE , Wintershall Holding GmbH
Inventor： Beata Brockmann , Andrea Herold , Oskar Zelder , Stefan Haefner , Christian Fleck , Hartwig Schröder , Mari Granström , Julia Kristine Schmidt
CPC classification number: C12P19/04 , C12N9/1051 , C12Y204/01034
Abstract: The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that said genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-β-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity or the use of such a polypeptide for producing β-glucans. Furthermore, the present invention relates to methods for producing β-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity thereby increasing the expression of said polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity into a microorganism being able to synthesize β-glucans.
Abstract translation: 本发明涉及能够产生β-葡聚糖的转基因微生物，其特征在于所述遗传修饰的微生物过表达（i）编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸和/或（ ii）与相同菌株的相应未修饰的对照微生物相比，具有1,3-葡聚糖 - D-葡聚糖合酶活性的多肽。 本发明还涉及编码具有1,3-葡萄糖 - D-葡聚糖合酶活性的多肽的多核苷酸的用途或这种多肽用于生产葡聚糖的用途。 此外，本发明涉及生产葡聚糖的方法，包括在编码具有1,3-葡萄糖-D-葡聚糖合酶活性的多肽的多核苷酸上游引入启动子，从而增加所述多核苷酸的表达，或 编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸能够合成葡聚糖。
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14.发明申请公开(公告)号：US20140127764A1
公开(公告)日：2014-05-08
申请号：US14150206
申请日：2014-01-08
Applicant: BASF SE
Inventor： Hartwig Schröder , Stefan Haefner , Gregory Von Abendroth , Rajan Hollmann , Aline Raddatz , Hansgeorg Ernst , Hans Gurski
IPC: C12P7/46
CPC classification number: C12P7/46 , C12N1/32 , C12N9/1029 , C12R1/01 , C12Y203/01054
Abstract: The present invention relates to bacterial strains, capable of utilizing glycerol as a carbon source for the fermentative production of succinic acid, wherein said strains are genetically modified so that they comprise a deregulation of their endogenous pyruvate-formate-lyase enzyme activity, as well as to methods of producing organic acids, in particular succinic acid, by making use of such microorganism. The present invention also relates to the downstream processing of the produced organic acids by cation exchange chromatography.
Abstract translation: 本发明涉及能够利用甘油作为发酵生产琥珀酸的碳源的细菌菌株，其中所述菌株被遗传修饰，使得它们包含其内源性丙酮酸 - 甲酸裂解酶活性的失调，以及 涉及通过利用这种微生物生产有机酸，特别是琥珀酸的方法。 本发明还涉及通过阳离子交换层析对所生产的有机酸的下游加工。
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公开(公告)号：US10731188B2
公开(公告)日：2020-08-04
申请号：US16235511
申请日：2018-12-28
Applicant: BASF SE
Inventor： Hartwig Schröder , Holger Hartmann , Qingzhao Wang , Shakir Siraj Ratani , Zheyuan Guo , Markus Pompejus
IPC: C12P13/06 , C07K14/245 , C12N1/20 , C12N9/06 , C12P7/04 , C12P7/46 , C12P7/56 , C12P13/08 , C12P13/20
Abstract: The present invention relates to a recombinant nucleic acid molecule, a recombinant microorganism, to a method for producing alanine and to the use of the recombinant nucleic acid molecule or the recombinant microorganism for the fermentative production of alanine.
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Patent Agency Ranking
公开(公告)号：US20160304917A1
公开(公告)日：2016-10-20
申请号：US14914855
申请日：2014-08-18
Applicant: BASF SE
Inventor： Joanna Martyna Krawczyk , Stefan Haefner , Hartwig Schröder , Oskar Zelder , Jonathan Thomas Fabarius
CPC classification number: C12P13/06 , C12N9/0016 , C12R1/01 , C12Y104/01001
Abstract: The present invention relates to a modified microorganism having, compared to its wildtype, an increased activity of the enzyme that is encoded by the alaD-gene. The present invention also relates to a method for producing an alanine and to the use of modified microorganisms.
Abstract translation: 本发明涉及一种经修饰的微生物，其与野生型相比具有增加由alaD基因编码的酶的活性。 本发明还涉及丙氨酸的生产方法和改性微生物的使用。
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17.发明授权Genetically modified microorganisms capable of producing β-glucans and methods for producing β-glucans 有权能够生产葡聚糖的转基因微生物和生产葡聚糖的方法公开(公告)号：US09388441B1
公开(公告)日：2016-07-12
申请号：US15074330
申请日：2016-03-18
Applicant: BASF SE , Wintershall Holding GmbH
Inventor： Beata Brockmann , Andrea Herold , Oskar Zelder , Stefan Haefner , Christian Fleck , Hartwig Schröder , Mari Granström , Julia Kristiane Schmidt
CPC classification number: C12P19/04 , C08B37/0024 , C08L5/00 , C12N9/1051 , C12N15/80 , C12P19/18 , C12Y204/01034 , Y02P20/52
Abstract: The present invention relates to genetically modified microorganisms capable of producing beta-glucans, characterized in that the genetically modified microorganism overexpresses (i) a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity, and/or (ii) a polypeptide having 1,3-β-D-glucan synthase-activity, compared to a corresponding non-modified control microorganism of the same strain. The present invention also relates to the use of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity or the use of such a polypeptide for producing β-glucans. Furthermore, the present invention relates to methods for producing β-glucans comprising the introduction of a promoter upstream of a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity thereby increasing the expression of the polynucleotide, or a polynucleotide encoding a polypeptide having 1,3-β-D-glucan synthase-activity into a microorganism being able to synthesize β-glucans.
Abstract translation: 本发明涉及能够产生β-葡聚糖的转基因微生物，其特征在于，遗传修饰的微生物过表达（i）编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸和/或（ ii）与相同菌株的相应未修饰的对照微生物相比，具有1,3-葡聚糖 - D-葡聚糖合酶活性的多肽。 本发明还涉及编码具有1,3-葡萄糖 - D-葡聚糖合酶活性的多肽的多核苷酸的用途或这种多肽用于生产葡聚糖的用途。 此外，本发明涉及生产葡聚糖的方法，包括在编码具有1,3-葡萄糖-D-葡聚糖合酶活性的多肽的多核苷酸上游引入启动子，从而增加多核苷酸的表达，或 编码具有1,3-葡聚糖合酶活性的多肽的多核苷酸能够合成葡聚糖。
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18.发明申请公开(公告)号：US20140141440A1
公开(公告)日：2014-05-22
申请号：US14084030
申请日：2013-11-19
Applicant: BASF SE
Inventor： Oskar Zelder , Birgit Hoff , Hartwig Schröder , Andrea Molt , Holger Hartmann , Klaus Ditrich , Michael Breuer , Rüdiger Reingruber , Jakob Weber
IPC: C12P17/04
Abstract: The invention pertains to/the field of production of natural products and, in particular, in the field of production of cornexistin and hydroxycornexistin. It provides polynucleotides encoding polypeptides involved in the biosynthesis of cornexistin and hydroxycornexistin as well as vectors and recombinant microorganisms comprising such polynucleotides. Also provided are methods for the production of natural products, in particular methods for the production of cornexistin and hydroxycornexistin, using such polynucleotides and polpeptides encoded therein, as well as vectors and recombinant microorganisms comprising such polynucleotides and polypeptides.
Abstract translation: 本发明涉及天然产物的生产领域，尤其涉及在玉米corn霉素和羟基角质素的生产领域。 它提供了编码涉及Cornexistin和羟基角质素的生物合成的多肽的多核苷酸以及包含这种多核苷酸的载体和重组微生物。 还提供了生产天然产物的方法，特别是使用这样的多核苷酸和其中编码的多肽以及包含这种多核苷酸和多肽的载体和重组微生物来生产玉米corn霉素和羟基角质柔比星的方法。
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公开(公告)号：US10513693B2
公开(公告)日：2019-12-24
申请号：US15127181
申请日：2015-03-18
Applicant: BASF SE
Inventor： Esther Dantas Costa , Oskar Zelder , Hartwig Schröder , Stefan Haefner , Joanna Martyna Krawczyk , Gregory Von Abendroth , Christian Riedele , Torsten Renz
IPC: C12N9/04 , C12N9/10 , C12P7/46 , C07K14/285
Abstract: The present invention relates to a process for producing an organic acid by fermentation, comprising the process steps: I) cultivating microorganisms in a culture medium to which are fed, as assimilable carbon sources, glycerol and a further carbonaceous compound, to allow the microorganisms to produce the organic acid, thereby obtaining a fermentation broth comprising the organic acid; II) recovering the organic acid or the salt thereof from the fermentation broth obtained in process step I); wherein, at least for a certain period of time in process step I), the consumption rate of the further carbonaceous compound (CRc.c.; in g per liter per hour) is lower than the maximum theoretical consumption rate of the further carbonaceous compound (CRc.c. max; in g per liter per hour).
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公开(公告)号：US20180030418A1
公开(公告)日：2018-02-01
申请号：US15127181
申请日：2015-03-18
Applicant: BASF SE
Inventor： Esther Dantas Costa , Oskar Zelder , Hartwig Schröder , Stefan Haefner , Joanna Martyna Krawczyk , Gregory Von Abendroth , Christian Riedele , Torsten Renz
CPC classification number: C12N9/0006 , C07K14/285 , C12N9/1029 , C12P7/46 , C12Y101/01027 , C12Y203/01054
Abstract: The present invention relates to a process for producing an organic acid by fermentation, comprising the process steps: I) cultivating microorganisms in a culture medium to which are fed, as assimilable carbon sources, glycerol and a further carbonaceous compound, to allow the microorganisms to produce the organic acid, thereby obtaining a fermentation broth comprising the organic acid; II) recovering the organic acid or the salt thereof from the fermentation broth obtained in process step I); wherein, at least for a certain period of time in process step I), the consumption rate of the further carbonaceous compound (CRc.c.; in g per liter per hour) is lower than the maximum theoretical consumption rate of the further carbonaceous compound (CRc.c. max; in g per liter per hour).
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