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公开(公告)号：US10823658B2
公开(公告)日：2020-11-03
申请号：US15992097
申请日：2018-05-29
Applicant: Becton, Dickinson and Company
Inventor： Eric D. Diebold , Keegan Owsley , Matthew Bahr
Abstract: Aspects of the present disclosure include methods for producing an output laser beam having two or more angularly deflected laser beams (e.g., for irradiating a sample in a flow stream) with a predetermined intensity profile. Systems for practicing the subject methods having a laser, an acousto-optic device, a radiofrequency generator and a controller for adjusting the amplitude of the radiofrequency drive signals to produce an output laser beam of angularly deflected laser beams with a predetermined intensity profile are also described.
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12.发明申请公开(公告)号：US20200309671A1
公开(公告)日：2020-10-01
申请号：US16817080
申请日：2020-03-12
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Matthew Bahr , Keegan Owsley
Abstract: Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.
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公开(公告)号：US20190234863A1
公开(公告)日：2019-08-01
申请号：US16382056
申请日：2019-04-11
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Eric Diebold , Keegan Owsley , Jonathan Lin
CPC classification number: G01N15/1434 , G01N15/1429 , G01N15/147 , G01N21/6402 , G01N21/6428 , G01N2015/1006 , G01N2015/1447 , G01N2015/149 , G01N2021/6439
Abstract: In one aspect, a method of sorting cells in a flow cytometry system is disclosed, which includes illuminating a cell with radiation having at least two optical frequencies shifted from one another by a radiofrequency to elicit fluorescent radiation from the cell, detecting the fluorescent radiation to generate temporal fluorescence data, and processing the temporal fluorescence data to arrive at a sorting decision regarding the cell without generating an image (i.e., a pixel-by-pixel image) of the cell based on the fluorescence data. In some cases, the sorting decision can be made with a latency less than about 100 microseconds. In some embodiments, the above method of sorting cells can have a sub-cellular resolution. In some embodiments, a single radiofrequency shift is employed to separate the optical frequencies while in other such embodiments a plurality of different radiofrequency shifts are employed.
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公开(公告)号：US11940372B2
公开(公告)日：2024-03-26
申请号：US17955060
申请日：2022-09-28
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Jonathan Lin , Matthew Bahr , Keegan Owsley
CPC classification number: G01N15/1475 , G01N15/147 , G06T7/11 , G06T7/136 , G06T7/66 , G06T7/70 , G01N2015/1006 , G01N2015/149 , G06T2207/10064 , G06T2207/30024
Abstract: Aspects of the present disclosure include a method for sorting cells of a sample based on an image of a cell in a flow stream. Methods according to certain embodiments include detecting light from a sample having cells in a flow stream, generating an image mask of a cell from the sample and sorting the cell based on the generated image mask. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to generate an image mask of a cell in a sample in a flow stream and to sort the cell based on the generated image mask are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for generating an image mask and for determining one or more features of the cell are also provided.
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公开(公告)号：US11940369B2
公开(公告)日：2024-03-26
申请号：US18214317
申请日：2023-06-26
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Eric D. Diebold , Keegan Owsley , Jonathan Lin
CPC classification number: G01N15/1434 , G01N15/14 , G01N15/1425 , G01N15/1429 , G01N15/147 , G01N15/1475 , G01N21/645 , G02B21/06 , G01N2015/1006 , G01N2015/1447 , G01N2015/1454 , G01N2021/6482
Abstract: Aspects of the disclosure include a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) with a top-hat-shaped excitation beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion to generate an image of the sample from the detected fluorescence. In another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency (e.g., a horizontal extent), which is generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency and detected fluorescence is used to analyze the sample.
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Patent Agency Ranking
公开(公告)号：US11692926B2
公开(公告)日：2023-07-04
申请号：US17745249
申请日：2022-05-16
Applicant: BECTON, DICKINSON AND COMPANY
Inventor： Eric D. Diebold , Keegan Owsley , Jonathan Lin
CPC classification number: G01N15/1434 , G01N15/14 , G01N15/147 , G01N15/1425 , G01N15/1429 , G01N15/1475 , G01N21/645 , G02B21/06 , G01N2015/1006 , G01N2015/1447 , G01N2015/1454 , G01N2021/6482
Abstract: In one aspect, the present teachings provide a system for performing cytometry that can be operated in three operational modes. In one operational mode, a fluorescence image of a sample is obtained by exciting one or more fluorophore(s) present in the sample by an excitation beam formed as a superposition of a top-hat-shaped beam with a plurality of beams that are radiofrequency shifted relative to one another. In another operational mode, a sample can be illuminated successively over a time interval by a laser beam at a plurality of excitation frequencies in a scanning fashion. The fluorescence emission from the sample can be detected and analyzed, e.g., to generate a fluorescence image of the sample. In yet another operational mode, the system can be operated to illuminate a plurality of locations of a sample concurrently by a single excitation frequency, which can be generated, e.g., by shifting the central frequency of a laser beam by a radiofrequency. For example, a horizontal extent of the sample can be illuminated by a laser beam at a single excitation frequency. The detected fluorescence radiation can be used to analyze the fluorescence content of the sample, e.g., a cell/particle.
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公开(公告)号：US11644407B2
公开(公告)日：2023-05-09
申请号：US17545325
申请日：2021-12-08
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Joseph Trotter , Keegan Owsley
CPC classification number: G16C20/20 , B07C5/3425 , B07C5/361 , G01N15/1425 , G01N15/1434 , G06V20/695 , G06V20/698 , G01N2015/149
Abstract: A cell sorting system that automatically generates a sorting strategy based on examples of target events provided by an operator. The target events can be selected using measurements ranging from traditional flow cytometry measurements to derived measurements that are computationally expensive to complex measurements such as images.
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公开(公告)号：US11579071B2
公开(公告)日：2023-02-14
申请号：US16887538
申请日：2020-05-29
Applicant: Becton, Dickinson and Company
Inventor： Jonathan Lin , Keegan Owsley , Matthew Bahr
IPC: G01N15/14
Abstract: Aspects of the present disclosure include methods for characterizing particles of a sample in a flow stream. Methods according to certain embodiments include generating frequency-encoded fluorescence data from a particle of a sample in a flow stream; and calculating phase-corrected spatial data of the particle by performing a transform of the frequency-encoded fluorescence data with a phase correction component. In certain embodiments, methods include generating an image of the particle in the flow stream based on the phase-corrected spatial data. Systems having a processor with memory operably coupled to the processor having instructions stored thereon, which when executed by the processor, cause the processor to calculate phase-corrected spatial data from frequency-encoded fluorescence data of a particle a flow stream are also described. Integrated circuit devices (e.g., field programmable gate arrays) having programming for practicing the subject methods are also provided.
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公开(公告)号：US20220390349A1
公开(公告)日：2022-12-08
申请号：US17699865
申请日：2022-03-21
Applicant: Becton, Dickinson and Company
Inventor： David Alexander Roberts , Christopher J. Wolf , Keegan Owsley
IPC: G01N15/14 , G01N33/49 , G01N33/493
Abstract: Methods of classifying flow cytometer data are provided. Methods of interest include receiving a first gate and flow cytometer data, expanding the first gate to generate a second gate, and determining sets of flow cytometer data encompassed by each of the first gate and the second gate to classify the flow cytometer data. In embodiments, methods also involve recording a subset of the classified flow cytometer data and optionally adjusting the first and/or second gates based on the recorded data. In some cases, the subject methods include sorting particles associated with the classified flow cytometer data based on the first and second gates. Systems and computer-readable storage media for practicing the invention are also provided.
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公开(公告)号：US11513058B2
公开(公告)日：2022-11-29
申请号：US17313457
申请日：2021-05-06
Applicant: Becton, Dickinson and Company
Inventor： Matthew Bahr , Keegan Owsley , Jizuo Zou
Abstract: Aspects of the present disclosure include methods for modulating an intensity profile of a laser beam. Methods according to certain embodiments include irradiating an acousto-optic device with a laser to generate an output laser beam having a plurality of angularly deflected laser beams, capturing an image of the output laser beam, determining an intensity profile of the output laser beam along a horizontal axis from the captured image and adjusting one or more parameters of a waveform inputted into the acousto-optic device in response to the determined intensity profile to generate an output laser beam having a modulated intensity profile. Systems having a laser, an acousto-optic device, an imaging sensor and a waveform generator as well as non-transitory computer readable storage medium with instructions for practicing the subject methods are also described.
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