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11.发明授权Isolation and characterization of the genomic DNA clones of ribosomal protein gene L25 in tobacco 失效烟草中核糖体蛋白基因L25的基因组DNA克隆的分离和表征公开(公告)号：US06783978B1
公开(公告)日：2004-08-31
申请号：US09714948
申请日：2000-11-20
申请人： Lifang Shi , Johnway Gao , Brian S. Hooker , Ziyu Dai
发明人： Lifang Shi , Johnway Gao , Brian S. Hooker , Ziyu Dai
IPC分类号： C12N1500
CPC分类号： C12N15/8234 , C12N15/8225
摘要： The promoter of the ribosomal protein gene L25 is operably linked to a structural gene. This chimeric gene is placed in an expression vector. The expression vector containing the chimeric gene is used to transform plants cells and plants. Seeds are obtained from the transformed plants. A product, such as a protein, encoded by the structural gene is isolated from the transformed plant cells and plants.
摘要翻译： 核糖体蛋白基因L25的启动子与结构基因可操作地连接。 将该嵌合基因置于表达载体中。 含有嵌合基因的表达载体用于转化植物细胞和植物。 从转化的植物获得种子。 从转化的植物细胞和植物中分离由结构基因编码的产物，如蛋白质。
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公开(公告)号：US06355864B1
公开(公告)日：2002-03-12
申请号：US09417019
申请日：1999-10-12
申请人： Lifang Shi , Ziyu Dai , Johnway Gao , Brian S. Hooker , Daniel B. Anderson
发明人： Lifang Shi , Ziyu Dai , Johnway Gao , Brian S. Hooker , Daniel B. Anderson
IPC分类号： C07H2104
CPC分类号： C12N15/8238 , C12N15/8216 , C12N15/8229 , C12N15/8237 , C12N15/8239
摘要： Synergistically functional, yet separable, cis-acting enhancer elements from the rpL34 promoter are disclosed. These enhancer elements of the instant invention may be used in combination with a plurality of promoters to increase gene expression without affecting the intrinsic specificity of the promoters. Also disclosed are methods for using the enhancer elements of the instant invention as well as vectors and transgenic plants comprising the enhancer elements.
摘要翻译： 公开了来自rpL34启动子的协同作用的，但可分离的顺式作用的增强子元件。 本发明的这些增强子元件可以与多个启动子组合使用以增加基因表达而不影响启动子的固有特异性。 还公开了使用本发明的增强子元件以及包含增强子元件的载体和转基因植物的方法。
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公开(公告)号：US06551798B2
公开(公告)日：2003-04-22
申请号：US09921942
申请日：2001-08-02
IPC分类号： C12N1563
摘要： The present invention provides the promoter clone discovery of an alpha-amylase gene of a starch utilizing yeast strain Schwanniomyces castellii. The isolated alpha-amylase promoter is an inducible promoter, which can regulate strong gene expression in starch culture medium.
摘要翻译： 本发明提供了使用酵母菌株施氏葡萄球菌（Schwanniomyces castellii）的淀粉的α-淀粉酶基因的启动子克隆发现。 分离的α-淀粉酶启动子是可诱导的启动子，可以调节淀粉培养基中的强基因表达。
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14.发明授权Method and apparatus for energy efficient self-aeration in chemical, biochemical, and wastewater treatment processes 失效化学，生化和废水处理过程中能量效率自曝气的方法和装置公开(公告)号：US06395175B1
公开(公告)日：2002-05-28
申请号：US09541868
申请日：2000-04-03
申请人： Johnway Gao , Rodney S. Skeen
发明人： Johnway Gao , Rodney S. Skeen
IPC分类号： C02F174
CPC分类号： B01F3/0446 , B01F5/0646 , B01F5/0655 , B01F5/0659 , B01F2215/0052 , C02F1/74 , C02F2301/063 , Y10S261/54 , Y10S261/75
摘要： The present invention is a pulse spilling self-aerator (PSSA) that has the potential to greatly lower the installation, operation, and maintenance cost associated with aerating and mixing aqueous solutions. Currently, large quantities of low-pressure air are required in aeration systems to support many biochemical production processes and wastewater treatment plants. Oxygen is traditionally supplied and mixed by a compressor or blower and a mechanical agitator. These systems have high-energy requirements and high installation and maintenance costs. The PSSA provides a mixing and aeration capability that can increase operational efficiency and reduce overall cost.
摘要翻译： 本发明是一种脉冲溢出自曝气（PSSA），其具有大大降低与曝气和混合水溶液相关的安装，操作和维护成本的潜力。 目前，曝气系统需要大量的低压空气来支持许多生化生产过程和污水处理厂。 氧气传统上由压缩机或鼓风机和机械搅拌器供应和混合。 这些系统具有高能量要求和高安装和维护成本。 PSSA提供混合和曝气功能，可以提高运营效率并降低总体成本。
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公开(公告)号：US07518710B2
公开(公告)日：2009-04-14
申请号：US11486619
申请日：2006-07-14
申请人： Johnway Gao , Jerome U. Gilberry , William A. Ivancic , John J. Kester , Joseph A. McClintock , Clark A. Morrow , Jason A. Schaefer
发明人： Johnway Gao , Jerome U. Gilberry , William A. Ivancic , John J. Kester , Joseph A. McClintock , Clark A. Morrow , Jason A. Schaefer
CPC分类号： G01N15/1463 , G01N2015/0261 , G01N2015/1488 , Y10T436/11 , Y10T436/115831
摘要： A biological and chemical detection system is provided that detects and identifies biological and/or chemical particulates of interest. The biological and chemical detection system comprises a collector, a first optical device, a second optical device and a processor. The collector is configured to deposit particulates drawn from a fluid stream onto a sample substrate to define a sample area. The first optical device derives first data relative to at least a portion of the sample area, which is analyzed to determine at least one field of view and/or specific target location. The second optical device then interrogates the sample area at each determined target location, e.g., using Raman spectroscopy, to produce interrogation data. The processor determines whether the sample area includes predetermined biological or chemical particulates of interest based upon an analysis of the interrogation data and triggers an event such as an alarm or message if the predetermined biological or chemical particulates of interest are identified.
摘要翻译： 提供了一种生物和化学检测系统，用于检测和识别感兴趣的生物和/或化学颗粒物。 生物和化学检测系统包括收集器，第一光学装置，第二光学装置和处理器。 收集器被配置为将从流体流中吸取的微粒沉积到样品基底上以限定样品区域。 第一光学装置相对于样本区域的至少一部分导出第一数据，其被分析以确定至少一个视场和/或特定目标位置。 然后，第二光学装置在每个确定的目标位置询问样品区域，例如使用拉曼光谱法，产生询问数据。 处理器基于询问数据的分析来确定样本区域是否包括预期的生物或化学颗粒物，并且如果识别出预定的感兴趣的生物或化学颗粒物，则触发诸如报警或消息的事件。
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公开(公告)号：US20090066934A1
公开(公告)日：2009-03-12
申请号：US11486619
申请日：2006-07-14
申请人： Johnway Gao , Jerome U. Gilberry , William A. Ivancic , John J. Kester , Joseph A. McClintock , Clark A. Morrow , Jason A. Schaefer
发明人： Johnway Gao , Jerome U. Gilberry , William A. Ivancic , John J. Kester , Joseph A. McClintock , Clark A. Morrow , Jason A. Schaefer
CPC分类号： G01N15/1463 , G01N2015/0261 , G01N2015/1488 , Y10T436/11 , Y10T436/115831
摘要： A biological and chemical detection system is provided that detects and identifies biological and/or chemical particulates of interest. The biological and chemical detection system comprises a collector, a first optical device, a second optical device and a processor. The collector is configured to deposit particulates drawn from a fluid stream onto a sample substrate to define a sample area. The first optical device derives first data relative to at least a portion of the sample area, which is analyzed to determine at least one field of view and/or specific target location. The second optical device then interrogates the sample area at each determined target location, e.g., using Raman spectroscopy, to produce interrogation data. The processor determines whether the sample area includes predetermined biological or chemical particulates of interest based upon an analysis of the interrogation data and triggers an event such as an alarm or message if the predetermined biological or chemical particulates of interest are identified.
摘要翻译： 提供了一种生物和化学检测系统，用于检测和识别感兴趣的生物和/或化学颗粒物。 生物和化学检测系统包括收集器，第一光学装置，第二光学装置和处理器。 收集器被配置为将从流体流中吸取的微粒沉积到样品基底上以限定样品区域。 第一光学装置相对于样本区域的至少一部分导出第一数据，其被分析以确定至少一个视场和/或特定目标位置。 然后，第二光学装置在每个确定的目标位置询问样品区域，例如使用拉曼光谱法，产生询问数据。 处理器基于询问数据的分析来确定样品区域是否包括预期的生物或化学颗粒物，并且如果识别出预定的感兴趣的生物或化学颗粒物，则触发诸如报警或消息的事件。
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17.发明授权Promoter sequence of 3-phosphoglycerate kinase gene 1 of lactic acid-producing fungus rhizopus oryzae and a method of expressing a gene of interest in fungal species 失效产生乳酸的真菌根霉的3-磷酸甘油酸激酶基因1的启动子序列和在真菌种中表达目的基因的方法公开(公告)号：US06465635B2
公开(公告)日：2002-10-15
申请号：US09536034
申请日：2000-03-27
申请人： Johnway Gao , Rodney S. Skeen
发明人： Johnway Gao , Rodney S. Skeen
IPC分类号： C07H2104
CPC分类号： C12N9/1217 , C07K14/37 , C12N15/80
摘要： The present invention provides the promoter clone discovery of phosphoglycerate kinase gene 1 of a lactic acid-producing filamentous fungal strain, Rhizopus oryzae. The isolated promoter can constitutively regulate gene expression under various carbohydrate conditions. In addition, the present invention also provides a design of an integration vector for the transformation of a foreign gene in Rhizopus oryzae.
摘要翻译： 本发明提供了产生乳酸的丝状真菌菌株，米根霉（Rhizopus oryzae）的磷酸甘油酸激酶基因1的启动子克隆发现。 分离的启动子可以在各种碳水化合物条件下组成型调节基因表达。 此外，本发明还提供了用于在米根霉（Rhizopus oryzae）中外源基因转化的整合载体的设计。
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公开(公告)号：US20100261280A1
公开(公告)日：2010-10-14
申请号：US11486939
申请日：2006-07-14
申请人： Rodney S. Black , Jeffrey P. Carpenter , Johnway Gao , Jerome U. Gilberry , John J. Kester , Clark A. Morrow
发明人： Rodney S. Black , Jeffrey P. Carpenter , Johnway Gao , Jerome U. Gilberry , John J. Kester , Clark A. Morrow
CPC分类号： G01N15/1463 , G01N2015/0261 , G01N2015/1488 , Y10T436/11 , Y10T436/115831
摘要： A biological and chemical detection system is provided that detects and identifies biological and/or chemical particulates of interest. The biological and chemical detection system comprises a collector, a first optical device, a second optical device and a processor. The collector is configured to deposit particulates drawn from a fluid stream onto a sample substrate to define a sample area. The first optical device derives first data relative to at least a portion of the sample area, which is analyzed to determine at least one field of view and/or specific target location. The second optical device then interrogates the sample area at each determined target location, e.g., using Raman spectroscopy, to produce interrogation data. The processor determines whether the sample area includes predetermined biological or chemical particulates of interest based upon an analysis of the interrogation data and triggers an event such as an alarm or message if the predetermined biological or chemical particulates of interest are identified.
摘要翻译： 提供了一种生物和化学检测系统，用于检测和识别感兴趣的生物和/或化学颗粒物。 生物和化学检测系统包括收集器，第一光学装置，第二光学装置和处理器。 收集器被配置为将从流体流中吸取的微粒沉积到样品基底上以限定样品区域。 第一光学装置相对于样本区域的至少一部分导出第一数据，其被分析以确定至少一个视场和/或特定目标位置。 然后，第二光学装置在每个确定的目标位置询问样品区域，例如使用拉曼光谱法，产生询问数据。 处理器基于询问数据的分析来确定样本区域是否包括预期的生物或化学颗粒物，并且如果识别出预定的感兴趣的生物或化学颗粒物，则触发诸如报警或消息的事件。
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公开(公告)号：US07532314B1
公开(公告)日：2009-05-12
申请号：US11486861
申请日：2006-07-14
申请人： Rodney S. Black , Jeffrey P. Carpenter , Johnway Gao , Jerome U. Gilberry , William A. Ivancic , John J. Kester , Clark A. Morrow
发明人： Rodney S. Black , Jeffrey P. Carpenter , Johnway Gao , Jerome U. Gilberry , William A. Ivancic , John J. Kester , Clark A. Morrow
CPC分类号： G01N15/1463 , G01N2015/0261 , G01N2015/1488 , Y10T436/11 , Y10T436/115831
摘要： A biological and chemical detection system is provided that detects and identifies biological and/or chemical particulates of interest. The biological and chemical detection system comprises a collector, a first optical device, a second optical device and a processor. The collector is configured to deposit particulates drawn from a fluid stream onto a sample substrate to define a sample area. The first optical device derives first data relative to at least a portion of the sample area, which is analyzed to determine at least one field of view and/or specific target location. The second optical device then interrogates the sample area at each determined target location, e.g., using Raman spectroscopy, to produce interrogation data. The processor determines whether the sample area includes predetermined biological or chemical particulates of interest based upon an analysis of the interrogation data and triggers an event such as an alarm or message if the predetermined biological or chemical particulates of interest are identified.
摘要翻译： 提供了一种生物和化学检测系统，用于检测和识别感兴趣的生物和/或化学颗粒物。 生物和化学检测系统包括收集器，第一光学装置，第二光学装置和处理器。 收集器被配置为将从流体流中吸取的微粒沉积到样品基底上以限定样品区域。 第一光学装置相对于样本区域的至少一部分导出第一数据，其被分析以确定至少一个视场和/或特定目标位置。 然后，第二光学装置在每个确定的目标位置询问样品区域，例如使用拉曼光谱法，产生询问数据。 处理器基于询问数据的分析来确定样本区域是否包括预期的生物或化学颗粒物，并且如果识别出预定的感兴趣的生物或化学颗粒物，则触发诸如报警或消息的事件。
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公开(公告)号：US20090103082A1
公开(公告)日：2009-04-23
申请号：US11486861
申请日：2006-07-14
申请人： Rodney S. Black , Jeffrey P. Carpenter , Johnway Gao , Jerome U. Gilberry , William A. Ivancic , John J. Kester , Clark A. Morrow
发明人： Rodney S. Black , Jeffrey P. Carpenter , Johnway Gao , Jerome U. Gilberry , William A. Ivancic , John J. Kester , Clark A. Morrow
CPC分类号： G01N15/1463 , G01N2015/0261 , G01N2015/1488 , Y10T436/11 , Y10T436/115831
摘要： A biological and chemical detection system is provided that detects and identifies biological and/or chemical particulates of interest. The biological and chemical detection system comprises a collector, a first optical device, a second optical device and a processor. The collector is configured to deposit particulates drawn from a fluid stream onto a sample substrate to define a sample area. The first optical device derives first data relative to at least a portion of the sample area, which is analyzed to determine at least one field of view and/or specific target location. The second optical device then interrogates the sample area at each determined target location, e.g., using Raman spectroscopy, to produce interrogation data. The processor determines whether the sample area includes predetermined biological or chemical particulates of interest based upon an analysis of the interrogation data and triggers an event such as an alarm or message if the predetermined biological or chemical particulates of interest are identified.
摘要翻译： 提供了一种生物和化学检测系统，用于检测和识别感兴趣的生物和/或化学颗粒物。 生物和化学检测系统包括收集器，第一光学装置，第二光学装置和处理器。 收集器被配置为将从流体流中吸取的微粒沉积到样品基底上以限定样品区域。 第一光学装置相对于样本区域的至少一部分导出第一数据，其被分析以确定至少一个视场和/或特定目标位置。 然后，第二光学装置在每个确定的目标位置询问样品区域，例如使用拉曼光谱法，产生询问数据。 处理器基于询问数据的分析来确定样品区域是否包括预期的生物或化学颗粒物，并且如果识别出预定的感兴趣的生物或化学颗粒物，则触发诸如报警或消息的事件。
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