公开(公告)号：US20160370266A1

公开(公告)日：2016-12-22

申请号：US15136763

申请日：2016-04-22

Applicant: Berkeley Lights, Inc.

Inventor： Mark P. White ,  Randall D. Lowe, JR.

IPC: G01N1/34 ,  B03C5/00 ,  G01N27/447 ,  C12Q1/68 ,  B01L3/00

CPC classification number: G01N1/34 ,  B01L3/502761 ,  B01L2200/0668 ,  B01L2300/0816 ,  B01L2300/0819 ,  B01L2300/0877 ,  B01L2400/0424 ,  B01L2400/0454 ,  B01L2400/0457 ,  B01L2400/086 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  C12Q1/6806 ,  G01N27/44704 ,  G01N27/44791

Abstract: Aspects of the present disclosure are directed to the manipulation of a cell nucleus in a micro-fluidic device as well as compositions, systems, and kits for performing such methods. In some aspects, the disclosure provides methods for placing one or more selected cell nuclei into an isolation region of a sequestration pen in a micro-fluidic device. The isolated nucleus/nuclei may then be retrieved from the isolation region of the sequestration pen and used in any desired downstream assay or process.

Abstract translation: 本公开的方面涉及微流体装置中的细胞核的操作以及用于执行这些方法的组合物，系统和试剂盒。 在一些方面，本公开提供了将一个或多个选定的细胞核放置在微流体装置中的隔离笔的隔离区域中的方法。 然后可以从隔离笔的隔离区域中取出分离的核/核，并用于任何所需的下游测定或过程。

公开(公告)号：US20160158748A1

公开(公告)日：2016-06-09

申请号：US14960010

申请日：2015-12-04

Applicant: BERKELEY LIGHTS, INC. ,  THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

Inventor： Ming-Chiang Wu ,  Jodi Tsu-An Loo ,  Shao Ning Pei ,  Gaetan L. Mathieu ,  Jian Gong ,  Randall D. Lowe, JR. ,  Justin K. Valley

IPC: B01L3/00 ,  G01N27/447

CPC classification number: B01L3/50273 ,  B01L3/502707 ,  B01L3/502715 ,  B01L3/502792 ,  B01L2200/06 ,  B01L2300/0654 ,  B01L2300/0816 ,  B01L2300/0858 ,  B01L2300/0877 ,  B01L2300/12 ,  B01L2300/165 ,  B01L2400/0427 ,  G01N27/44704 ,  G01N27/44743 ,  G01N27/44791

Abstract: Single-sided optoelectrowetting (SSOEW)-configured substrates are provided, as well as microfluidic devices that include such substrates. The substrates can include a planar electrode, a photoconductive (or photosensitive) layer, a dielectric layer (single-layer or composite), a mesh electrode, and a hydrophobic coating. Fluid droplets can be moved across the hydrophobic coating of such substrates in a light-actuated manner, upon the application of a suitable AC voltage potential across the substrate and the focusing of light into the photoconductive layer of the substrate in a location proximal to the droplets. Walls can be disposed upon the substrates to form the microfluidic devices. Together the walls and substrate can form a microfluidic circuit, through which droplets can be moved.

Abstract translation: 提供了单面光电注入（SSOEW）配置的基板，以及包括这种基板的微流体装置。 基板可以包括平面电极，光导（或感光）层，电介质层（单层或复合），网状电极和疏水涂层。 当在基板上施加合适的AC电压电位并且将光聚焦到基板的光电导层中时，流体液滴可以以光致致动的方式移动穿过这种基板的疏水涂层 。 壁可以设置在基底上以形成微流体装置。 壁和基底可以一起形成微流体电路，通过该微流体电路可以移动液滴。