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公开(公告)号：US07214536B2
公开(公告)日：2007-05-08
申请号：US10931246
申请日：2004-09-01
CPC分类号： C12Q1/683 , A01K2217/05 , C12N9/22 , C12N15/66 , C12Q1/6841 , Y10S530/824
摘要： An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes.
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公开(公告)号：US20050032223A1
公开(公告)日：2005-02-10
申请号：US10931246
申请日：2004-09-01
IPC分类号： A01K67/027 , C07H21/02 , C07H21/04 , C12N1/19 , C12N1/21 , C12N5/00 , C12N5/10 , C12N9/16 , C12N9/22 , C12N15/00 , C12N15/09 , C12N15/55 , C12N15/63 , C12N15/66 , C12N15/74 , C12N15/82 , C12N15/86 , C12P21/02 , C12P21/06 , C12Q1/68 , C12R1/865 , C12R1/91
CPC分类号： C12Q1/683 , A01K2217/05 , C12N9/22 , C12N15/66 , C12Q1/6841 , Y10S530/824
摘要： An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes.
摘要翻译： 提供编码酶I-SceI的分离的DNA。 DNA序列可以并入克隆和表达载体，转化细胞系和转基因动物中。 载体可用于基因作图和基因定点插入。
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13.发明申请公开(公告)号：US20090081788A1
公开(公告)日：2009-03-26
申请号：US12000888
申请日：2007-12-18
IPC分类号： C12N5/06
CPC分类号： C12Q1/683 , A01K2217/05 , C12N9/22 , C12N15/66 , C12Q1/6841 , Y10S530/824
摘要： An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes.
摘要翻译： 提供编码酶I-SceI的分离的DNA。 DNA序列可以并入克隆和表达载体，转化细胞系和转基因动物中。 载体可用于基因作图和基因定点插入。
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公开(公告)号：US07309605B1
公开(公告)日：2007-12-18
申请号：US10820843
申请日：2004-04-09
CPC分类号： C12Q1/683 , A01K2217/05 , C12N9/22 , C12N15/66 , C12Q1/6841 , Y10S530/824
摘要： An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes.
摘要翻译： 提供编码酶I-SceI的分离的DNA。 DNA序列可以并入克隆和表达载体，转化细胞系和转基因动物中。 载体可用于基因作图和基因定点插入。
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公开(公告)号：US06395959B1
公开(公告)日：2002-05-28
申请号：US08643732
申请日：1996-05-06
IPC分类号： A01K67027
CPC分类号： C12N9/22 , A01K2217/05 , C12N15/66 , C12Q1/683 , C12Q1/6841
摘要： An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes.
摘要翻译： 提供编码酶I-SceI的分离的DNA。 DNA序列可以并入克隆和表达载体，转化细胞系和转基因动物中。 载体可用于基因作图和基因定点插入。
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公开(公告)号：US5866361A
公开(公告)日：1999-02-02
申请号：US465273
申请日：1995-06-05
IPC分类号： A01K67/027 , C07H21/02 , C07H21/04 , C12N1/19 , C12N1/21 , C12N5/00 , C12N5/10 , C12N9/16 , C12N9/22 , C12N15/00 , C12N15/09 , C12N15/55 , C12N15/63 , C12N15/66 , C12N15/74 , C12N15/82 , C12N15/86 , C12P21/02 , C12P21/06 , C12Q1/68 , C12R1/865 , C12R1/91 , C12N9/14
CPC分类号： C12Q1/683 , C12N15/66 , C12N9/22 , C12Q1/6841 , A01K2217/05 , Y10S530/824
摘要： An isolated DNA encoding the enzyme I-SceI is provided. The DNA sequence can be incorporated in cloning and expression vectors, transformed cell lines and transgenic animals. The vectors are useful in gene mapping and site-directed insertion of genes.
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公开(公告)号：US20140121115A1
公开(公告)日：2014-05-01
申请号：US13725798
申请日：2012-12-21
申请人： Sylvain Arnould , Sylvia Bruneau , Jean-Pierre Cabaniols , Patrick Chames , Andre Choulika , Phillipe Duchateau , Jean-Charles Epinat , Agnes Gouble , Emmanuel Lacroix , Frederic Paques , Christophe Perez-Michaut , Julianne Smith , Davie Sourdive
发明人： Sylvain Arnould , Sylvia Bruneau , Jean-Pierre Cabaniols , Patrick Chames , Andre Choulika , Phillipe Duchateau , Jean-Charles Epinat , Agnes Gouble , Emmanuel Lacroix , Frederic Paques , Christophe Perez-Michaut , Julianne Smith , Davie Sourdive
IPC分类号： C12N15/10
CPC分类号： C12N15/907 , A01K67/0275 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/105 , A01K2267/03 , A01K2267/0337 , A61K38/00 , A61K38/1709 , A61K38/465 , A61K48/00 , A61K48/0058 , C07K14/435 , C07K2319/81 , C12N7/00 , C12N9/22 , C12N15/1058 , C12N15/8509 , C12N15/86 , C12N15/90 , C12N2730/10143 , C12N2799/022 , C12N2800/80 , C12N2830/002 , C12N2830/55 , C12N2840/20 , C12N2840/44 , C12Y301/00 , C12Y301/21004
摘要： New rare-cutting endonucleases, also called custom-made meganucleases, which recognize and cleave a specific nucleotide sequence, derived polynucleotide sequences, recombinant vector cell, animal, or plant comprising said polynucleotide sequences, process for producing said rare-cutting endonucleases and any use thereof, more particularly, for genetic engineering, antiviral therapy and gene therapy.
摘要翻译： 新的稀释内切核酸酶，也称为定制的大范围核酸酶，其识别和切割特定的核苷酸序列，衍生的多核苷酸序列，包含所述多核苷酸序列的重组载体细胞，动物或植物，用于产生所述稀少内切核酸酶的方法和任何用途 更具体地，涉及基因工程，抗病毒治疗和基因治疗。
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18.发明授权I-Sce I induced gene replacement and gene conversion in embryonic stem cells 失效I-Sce I诱导胚胎干细胞中的基因替换和基因转化公开(公告)号：US06566579B1
公开(公告)日：2003-05-20
申请号：US09116834
申请日：1998-07-17
申请人： Frederic Jaisser , Michel Cohen-Tannoudji , Sylvie Robine , Andre Choulika , Daniel Louvard , Charles Babinet
发明人： Frederic Jaisser , Michel Cohen-Tannoudji , Sylvie Robine , Andre Choulika , Daniel Louvard , Charles Babinet
IPC分类号： G01N3300
CPC分类号： C07K14/4735
摘要： The invention relates to methods of introducing a heterologous DNA sequence into a mouse embryonic stem cell wherein the DNA sequence is inserted by homologous recombination into a villin gene/I-SceI hybrid by creating a double strand break with I-SceI meganuclease. Subsequently, the mouse embryonic stem cells can be used to generate a transgenic mouse comprising the heterologous DNA sequence. Additionally, the methods can be used for gene replacement in ovo where a mouse oocyte containing a villin gene/I-SceI hybrid within its genome exists or is first generated. More generally, the methods can be used for the targeted insertion of a heterologous DNA sequence into any cell containing a villin gene/I-SceI hybrid sequence within its genome.
摘要翻译： 本发明涉及将异源DNA序列引入小鼠胚胎干细胞的方法，其中通过与I-SceI大范围核酸酶产生双链断裂，将DNA序列通过同源重组插入到绒毛蛋白基因/ I-SceI杂交体中。 随后，小鼠胚胎干细胞可用于产生包含异源DNA序列的转基因小鼠。 此外，该方法可用于卵巢中的基因替换，其中在其基因组内含有绒毛蛋白基因/ I-SceI杂交体的小鼠卵母细胞存在或首先产生。 更一般地，该方法可用于将异源DNA序列靶向插入到其基因组内含有绒毛蛋白基因/ I-SceI杂交序列的任何细胞中。
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公开(公告)号：US20060206949A1
公开(公告)日：2006-09-14
申请号：US10543556
申请日：2004-01-28
申请人： Sylvain Arnould , Slyvia Bruneau , Jean-Pierre Cabaniols , Patrick Chames , Andre Choulika , Philippe Duchateau , Jean-Charles Epinat , Agnes Gouble , Emmanuel Lacroix , Frederic Pagues , Julianne Smith , Christophe Perez-Michaut
发明人： Sylvain Arnould , Slyvia Bruneau , Jean-Pierre Cabaniols , Patrick Chames , Andre Choulika , Philippe Duchateau , Jean-Charles Epinat , Agnes Gouble , Emmanuel Lacroix , Frederic Pagues , Julianne Smith , Christophe Perez-Michaut
CPC分类号： C12N15/907 , A01K67/0275 , A01K67/0278 , A01K2207/15 , A01K2217/00 , A01K2217/05 , A01K2227/105 , A01K2267/03 , A01K2267/0337 , A61K38/00 , A61K38/1709 , A61K38/465 , A61K48/00 , A61K48/0058 , C07K14/435 , C07K2319/81 , C12N7/00 , C12N9/22 , C12N15/1058 , C12N15/8509 , C12N15/86 , C12N15/90 , C12N2730/10143 , C12N2799/022 , C12N2800/80 , C12N2830/002 , C12N2830/55 , C12N2840/20 , C12N2840/44 , C12Y301/00 , C12Y301/21004
摘要： New rare-cutting endonucleases, also called custom-made meganucleases, which recognize and cleave a specific nucleotide sequence, derived polynucleotide sequences, recombinant vector cell, animal, or plant comprising said polynucleotide sequences, process for producing said rare-cutting endonucleases and any use thereof, more particularly, for genetic engineering, antiviral therapy and gene therapy.
摘要翻译： 新的稀释内切核酸酶，也称为定制的大范围核酸酶，其识别和切割特定的核苷酸序列，衍生的多核苷酸序列，包含所述多核苷酸序列的重组载体细胞，动物或植物，用于生产所述稀少内切核酸酶的方法和任何用途 更具体地，涉及基因工程，抗病毒治疗和基因治疗。
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公开(公告)号：US07098031B2
公开(公告)日：2006-08-29
申请号：US10242664
申请日：2002-09-13
CPC分类号： A01K67/0275 , A01K2217/05 , C12N15/90 , C12N2800/108 , C12N2800/80 , C12N2830/00 , C12N2830/008 , C12N2830/85 , C12N2840/203
摘要： The present invention concerns a method for in vivo generation of a linear polynucleotide with 5′ and 3′ free ends from a vector having no free end, said linear polynucleotide being integrated into the host cell genome. The vector having no free end according to the present invention comprise the polynucleotide to be linearized or excised flanked by a cleavage site, said cleavage site being preferably not found in the host cell genome. The present invention also relates to the resulting cells and their uses, for example for production of proteins or other genes, biomolecules, biomaterials, transgenic plants, vaccines, transgenic animals or for treatment or prophylaxis of a condition or disorder in an individual.
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