公开(公告)号：US20170151558A1

公开(公告)日：2017-06-01

申请号：US15351354

申请日：2016-11-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen ,  Donald A. Masquelier ,  Kevin D. Ness ,  Benjamin J. Hindson ,  Anthony J. Makarewicz, JR. ,  Erin R. Chia ,  Billy W. Colston, JR. ,  Serge Saxonov ,  Svilen S. Tzonev ,  Michael Y. Lucero ,  Ryan T. Koehler

IPC: B01L3/00 ,  C12Q1/68 ,  B01L9/00

CPC classification number: B01L3/50273 ,  B01F3/0807 ,  B01F5/0085 ,  B01F5/0256 ,  B01F13/0062 ,  B01F13/0071 ,  B01F13/1022 ,  B01L3/502 ,  B01L3/502761 ,  B01L3/502784 ,  B01L3/52 ,  B01L9/527 ,  B01L2200/025 ,  B01L2200/0636 ,  B01L2200/0647 ,  B01L2200/0673 ,  B01L2200/14 ,  B01L2200/16 ,  B01L2300/0609 ,  B01L2300/0681 ,  B01L2300/0816 ,  B01L2300/0829 ,  B01L2300/0861 ,  B01L2400/049 ,  C12Q1/686 ,  G01N35/08

Abstract: System, including methods, apparatus, and kits, for forming emulsions. In an exemplary method of generating droplets, a device may be selected that includes a plurality of emulsion-formation units each including a sample well, a continuous-phase well, a droplet well, and a channel network that fluidically interconnects the wells and creates a droplet-generation region. A discrete volume of sample-containing fluid may be placed into the sample well of each emulsion-formation unit, and a discrete volume of continuous-phase fluid into the continuous-phase well of each emulsion-formation unit. Pressure may be applied to the device with a fluidics assembly after the step of placing, such that the plurality of emulsion-formation units generate droplets in parallel with one another. A pressure signal may be detected from the fluidics assembly. Application of the pressure may be stopped when the pressure signal indicates that a sample well is empty.