Patent search ap:("Bio-Rad Laboratories Page Inc.") AND inv:"Ryan T. Koehler"

1.

发明申请
PARTITION-BASED METHOD OF ANALYSIS 有权

公开(公告)号：US20220008914A1

公开(公告)日：2022-01-13

申请号：US17486667

申请日：2021-09-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, JR. , Erin R. Chia , Billy W. Colston, JR. , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler

IPC: B01L3/00 , B01F3/08 , B01F13/00 , B01F13/10 , B01L9/00 , C12Q1/686

Abstract: Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.

2.

发明申请
SYSTEM FOR GENERATING DROPLETS WITH PRESSURE MONITORING 有权

公开(公告)号：US20170151558A1

公开(公告)日：2017-06-01

申请号：US15351354

申请日：2016-11-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, JR. , Erin R. Chia , Billy W. Colston, JR. , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler

IPC: B01L3/00 , C12Q1/68 , B01L9/00

CPC classification number: B01L3/50273 , B01F3/0807 , B01F5/0085 , B01F5/0256 , B01F13/0062 , B01F13/0071 , B01F13/1022 , B01L3/502 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L9/527 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , C12Q1/686 , G01N35/08

Abstract: System, including methods, apparatus, and kits, for forming emulsions. In an exemplary method of generating droplets, a device may be selected that includes a plurality of emulsion-formation units each including a sample well, a continuous-phase well, a droplet well, and a channel network that fluidically interconnects the wells and creates a droplet-generation region. A discrete volume of sample-containing fluid may be placed into the sample well of each emulsion-formation unit, and a discrete volume of continuous-phase fluid into the continuous-phase well of each emulsion-formation unit. Pressure may be applied to the device with a fluidics assembly after the step of placing, such that the plurality of emulsion-formation units generate droplets in parallel with one another. A pressure signal may be detected from the fluidics assembly. Application of the pressure may be stopped when the pressure signal indicates that a sample well is empty.

3.

发明申请
MULTIPLEXED DIGITAL ASSAY FOR VARIANT AND NORMAL FORMS OF A GENE OF INTEREST 有权
Title translation: 用于变异基因的变体和正常形式的多重数字测定

公开(公告)号：US20140274799A1

公开(公告)日：2014-09-18

申请号：US14215516

申请日：2014-03-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ryan T. Koehler , Svilen S. Tzonev

IPC: C12Q1/68

CPC classification number: C12Q1/6886 , C12Q1/6851 , C12Q1/686 , C12Q2600/156 , C12Q2535/125 , C12Q2537/143 , C12Q2537/165 , C12Q2563/159 , C12Q2537/16 , C12Q2545/101

Abstract: System, including methods, apparatus, and compositions, for performing a multiplexed digital assay of at least three targets. In an exemplary method, partitions may be formed each including a portion of a same sample. The sample may include a first target that is a reference sequence, a second target that is a variant sequence from a gene of interest, and a third target that is a normal sequence from the gene of interest. The target may be amplified in the partitions and amplification data may be collected. A level of the second target may be determined. A copy number of the third target may be determined from a level of the first target and a level of the third target.

Abstract translation: 用于执行至少三个目标的多路复用数字测定的系统，包括方法，装置和组合物。在示例性方法中，可以形成每个包括相同样品的一部分的分隔。样品可以包括作为参考序列的第一靶，作为来自目标基因的变体序列的第二靶，以及来自目的基因的正常序列的第三靶。可以在分区中放大目标，并且可以收集放大数据。可以确定第二目标的水平。可以从第一目标的级别和第三目标的级别确定第三目标的副本号码。

4.

发明申请
DROPLET-BASED ANALYSIS METHOD 审中-公开

公开(公告)号：US20180147573A1

公开(公告)日：2018-05-31

申请号：US15707908

申请日：2017-09-18

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz, Jr. , Erin R. Chia , Billy W. Colston, Jr. , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler

IPC: B01L3/00 , B01L9/00 , B01F13/00 , C12Q1/686 , B01F13/10 , B01F3/08 , B01F5/02 , B01F5/00 , G01N35/08

CPC classification number: B01L3/50273 , B01F3/0807 , B01F5/0085 , B01F5/0256 , B01F13/0062 , B01F13/0071 , B01F13/1022 , B01L3/502 , B01L3/502761 , B01L3/502784 , B01L3/52 , B01L9/527 , B01L2200/025 , B01L2200/0636 , B01L2200/0647 , B01L2200/0673 , B01L2200/14 , B01L2200/16 , B01L2300/0609 , B01L2300/0681 , B01L2300/0816 , B01L2300/0829 , B01L2300/0861 , B01L2400/049 , C12Q1/686 , G01N35/08

Abstract: Devices and methods for generating droplets. An exemplary device comprises a substantially planar base portion including a bottom surface having a plurality of microfluidic channels formed therein as recessed regions of the bottom surface. The device also comprises a plurality of protrusions projecting from a top surface of the base portion and each formed integrally with the base portion. The device further comprises a sample well, a carrier well, and a droplet well. Each well has an upper portion created by one of the protrusions. A cover layer is attached to the bottom surface of the base portion and seals a bottom side of each microfluidic channel.

5.

发明授权
Multiplexed digital assay for variant and normal forms of a gene of interest 有权
Title translation: 用于感兴趣的基因的变体和正常形式的多重数字测定

公开(公告)号：US09458511B2

公开(公告)日：2016-10-04

申请号：US14215516

申请日：2014-03-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ryan T. Koehler , Svilen S. Tzonev

IPC: C12Q1/68 , C12P19/34

CPC classification number: C12Q1/6886 , C12Q1/6851 , C12Q1/686 , C12Q2600/156 , C12Q2535/125 , C12Q2537/143 , C12Q2537/165 , C12Q2563/159 , C12Q2537/16 , C12Q2545/101

Abstract: System, including methods, apparatus, and compositions, for performing a multiplexed digital assay of at least three targets. In an exemplary method, partitions may be formed each including a portion of a same sample. The sample may include a first target that is a reference sequence, a second target that is a variant sequence from a gene of interest, and a third target that is a normal sequence from the gene of interest. The target may be amplified in the partitions and amplification data may be collected. A level of the second target may be determined. A copy number of the third target may be determined from a level of the first target and a level of the third target.

Abstract translation: 用于执行至少三个目标的多路复用数字测定的系统，包括方法，装置和组合物。在示例性方法中，可以形成每个包括相同样品的一部分的分隔。样品可以包括作为参考序列的第一靶，作为来自目标基因的变体序列的第二靶，以及来自目的基因的正常序列的第三靶。可以在分区中放大目标，并且可以收集放大数据。可以确定第二目标的水平。可以从第一目标的级别和第三目标的级别确定第三目标的副本号码。

6.

发明公开
PARTITION-BASED METHOD OF ANALYSIS 审中-公开

公开(公告)号：US20230372935A1

公开(公告)日：2023-11-23

申请号：US18362530

申请日：2023-07-31

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Amy L. Hiddessen , Donald A. Masquelier , Kevin D. Ness , Benjamin J. Hindson , Anthony J. Makarewicz , Erin R. Chia , Billy W. Colston , Serge Saxonov , Svilen S. Tzonev , Michael Y. Lucero , Ryan T. Koehler

IPC: B01L3/00 , B01F23/41 , B01F33/81 , B01F33/3011 , B01L9/00 , C12Q1/686

CPC classification number: B01L3/50273 , B01F23/41 , B01F33/813 , B01F33/3011 , B01L3/502 , B01L3/502784 , B01L3/52 , B01L9/527 , C12Q1/686 , B01F25/23

Abstract: Methods of partition-based analysis. In an exemplary method, a device having a port fluidically connected to a chamber may be selected. A sample-containing fluid may be placed into the port. The sample-containing fluid may be moved from the port to the chamber. Partitions of the sample-containing fluid may be formed. A monolayer of the partitions in the chamber may be created. At least a portion of the monolayer may be imaged.