公开(公告)号：US10954495B2

公开(公告)日：2021-03-23

申请号：US16101895

申请日：2018-08-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yan Wang

IPC: C12N9/12 ,  C07K14/00 ,  C07K19/00 ,  C12P19/34 ,  C12N9/96

Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.

公开(公告)号：US10301675B2

公开(公告)日：2019-05-28

申请号：US14926461

申请日：2015-10-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiao-Song Gong ,  Yan Wang

IPC: C12Q1/686 ,  C12Q1/6844 ,  C07K14/195

Abstract: In one aspect, compositions and methods for reducing reverse transcriptase (RT) inhibition in RT-PCR are provided. In some embodiments, the RT inhibition reducer is a phosphorothioate oligodeoxycytosine (SdC), phosphorothioate oligodeoxyadenine (SdA), phosphorothioate oligodeoxythymine (SdT), or phosphorothioate oligodeoxyguanosine (SdG).

公开(公告)号：US09200318B2

公开(公告)日：2015-12-01

申请号：US13681326

申请日：2012-11-19

Applicant: Bio Rad Laboratories, Inc.

Inventor： Xiao-Song Gong ,  Yan Wang

IPC: C12P19/34 ,  C12Q1/68 ,  C07K14/195 ,  C07H21/02 ,  C07H21/04

CPC classification number: C12Q1/686 ,  C07K14/195 ,  C12Q1/6844 ,  C12Q2527/127 ,  C12Q2527/125 ,  C12Q2521/307

Abstract: The present invention provides a method for amplifying a nucleic acid molecule. The method involves mixing an RNA template with a composition having a reverse transcriptase, a DNA polymerase and a RT inhibition reducer. The RT inhibition reducer can be Sso7d, Sac7d, Sac7e, Sso7e, AluI methylase, suramin, a phosphorothioate oligodeoxycytosine, a phosphorothioate oligodeoxyadenine, a phosphorothioate oligodeoxythymine or poly(rA)(dT). The mixing forms a mixture that is incubated under conditions sufficient to synthesize a DNA molecule complementary to all or a portion of the RNA template, thereby amplifying the nucleic acid molecule.

Abstract translation: 本发明提供了扩增核酸分子的方法。 该方法包括将RNA模板与具有逆转录酶，DNA聚合酶和RT抑制减少剂的组合物混合。 RT抑制减少剂可以是Sso7d，Sac7d，Sac7e，Sso7e，AluI甲基化酶，苏拉明，硫代磷酸寡脱氧胞嘧啶，硫代磷酸寡脱氧腺嘌呤，硫代磷酸寡脱氧胸苷或聚（rA）（dT）。 混合形成混合物，其在足以合成与所有或部分RNA模板互补的DNA分子的条件下孵育，从而扩增核酸分子。

公开(公告)号：US20140377767A1

公开(公告)日：2014-12-25

申请号：US14482756

申请日：2014-09-10

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiao-Song Gong ,  John Sullivan ,  Rongdian Fu ,  Yan Wang ,  Evan H. Bursey ,  Man Cheng

IPC: C12P19/34 ,  C12Q1/68

CPC classification number: C12P19/34 ,  C07K16/40 ,  C07K2317/56 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2319/80 ,  C12N9/1252 ,  C12Q1/6804 ,  C12Q1/686 ,  C12Q2531/113 ,  C12Q2527/125 ,  C12Q2521/101

Abstract: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonucleases activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.

Abstract translation: 本发明提供了提高核酸扩增反应效率的方法和组合物。 本发明包括与野生型聚合酶相比显示增加的活性以及降低的核酸外切酶活性的杂合聚合酶。 本发明还包括用于进行核酸合成和扩增反应的方法，组合物和试剂盒，其中引物的非特异性扩增减少。

公开(公告)号：US08895283B2

公开(公告)日：2014-11-25

申请号：US13850048

申请日：2013-03-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yan Wang

IPC: C12N9/12 ,  C07K1/00 ,  C07K14/00 ,  C07K19/00 ,  C12P19/34 ,  C12N9/96

CPC classification number: C12N9/1276 ,  C07K14/00 ,  C07K14/001 ,  C07K19/00 ,  C07K2319/80 ,  C12N9/1252 ,  C12N9/96 ,  C12P19/34 ,  C12Y207/07007 ,  C12Y207/07049 ,  Y02P20/52

Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.

Abstract translation: 本发明提供了改进的新型核酸修饰酶的产生。 改进是以增强酶结合和催化修饰核酸的能力的方式将序列非特异性核酸结合结构域与酶的融合。

公开(公告)号：US12065645B2

公开(公告)日：2024-08-20

申请号：US18125661

申请日：2023-03-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiao-Song Gong ,  Yan Wang

IPC: C12N9/12 ,  C12N9/22 ,  C12N15/10 ,  C12Q1/686 ,  C12Q1/6844

CPC classification number: C12N15/1096 ,  C12N9/22 ,  C12Q1/686 ,  C12N2770/00022 ,  C12Q1/6844 ,  C12Q1/6844 ,  C12Q2521/107 ,  C12Q2521/327

Abstract: Hybrid reverse transcriptases formed from portions of FLVRT and MLVRT are provided.

公开(公告)号：US10787694B2

公开(公告)日：2020-09-29

申请号：US15977254

申请日：2018-05-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xiao-Song Gong ,  John Sullivan ,  Rongdian Fu ,  Yan Wang ,  Evan H. Bursey ,  Man Cheng

IPC: C07K19/00 ,  C12Q1/6804 ,  C12Q1/686 ,  C12P19/34 ,  C07K16/40 ,  C12N9/12

Abstract: The present invention provides methods and compositions for improving the efficiency of nucleic acid amplification reactions. The invention encompasses hybrid polymerases that show increased processivity over wild type polymerases as well as decreased exonucleases activity. The invention also encompasses methods, compositions and kits for conducting nucleic acid synthesis and amplification reactions in which non-specific amplification of primers is reduced.

公开(公告)号：US10577593B2

公开(公告)日：2020-03-03

申请号：US15632733

申请日：2017-06-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Peter B. Vander Horn ,  Yan Wang

IPC: C12N9/12

Abstract: The invention provides novel compositions with polymerase activity and methods of using the compositions.

公开(公告)号：US10066218B2

公开(公告)日：2018-09-04

申请号：US15264345

申请日：2016-09-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yan Wang

IPC: C12N9/12 ,  C07K14/00

CPC classification number: C12N9/1276 ,  C07K14/00 ,  C07K14/001 ,  C07K19/00 ,  C07K2319/80 ,  C12N9/1252 ,  C12N9/96 ,  C12P19/34 ,  C12Y207/07007 ,  C12Y207/07049 ,  Y02P20/52

Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.

公开(公告)号：US20170198267A1

公开(公告)日：2017-07-13

申请号：US15264345

申请日：2016-09-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Yan Wang

IPC: C12N9/12 ,  C07K14/00

CPC classification number: C12N9/1276 ,  C07K14/00 ,  C07K14/001 ,  C07K19/00 ,  C07K2319/80 ,  C12N9/1252 ,  C12N9/96 ,  C12P19/34 ,  C12Y207/07007 ,  C12Y207/07049 ,  Y02P20/52

Abstract: This invention provides for an improved generation of novel nucleic acid modifying enzymes. The improvement is the fusion of a sequence-non-specific nucleic-acid-binding domain to the enzyme in a manner that enhances the ability of the enzyme to bind and catalytically modify the nucleic acid.