公开(公告)号：US11268887B2

公开(公告)日：2022-03-08

申请号：US16733137

申请日：2020-01-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin Miller ,  Brian Hutchison ,  Andrew Wilson ,  Jonathan William Larson ,  Qun Zhong ,  Yevgeny Yurkovetsky ,  Darren Roy Link ,  Mark Weary

IPC: G01N1/38 ,  F16K99/00 ,  B01L3/02 ,  B01L3/00 ,  B01F15/00 ,  B01F13/00 ,  F17D3/01 ,  G01N27/447 ,  G01N21/03 ,  G01N21/17

Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.

公开(公告)号：US10551382B2

公开(公告)日：2020-02-04

申请号：US16199822

申请日：2018-11-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Michael L. Samuels

IPC: G01N33/58 ,  G01N33/573 ,  B01F5/06 ,  B01J19/00 ,  B01F13/00 ,  B01L3/00 ,  B01F3/08 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  B01L7/00 ,  G01N21/64

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US10527529B2

公开(公告)日：2020-01-07

申请号：US15674074

申请日：2017-08-10

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Miller ,  Brian Hutchison ,  Andrew Wilson ,  Jonathan William Larson ,  Qun Zhong ,  Yevgeny Yurkovetsky ,  Darren Roy Link ,  Mark Weary

IPC: B01L3/02 ,  G01N1/38 ,  B01L3/00 ,  F17D3/01 ,  G01N27/447 ,  F16K99/00 ,  G01N21/03 ,  G01N21/17

Abstract: The invention provides methods for assessing one or more predetermined characteristics or properties of a microfluidic droplet within a microfluidic channel, and regulating one or more fluid flow rates within that channel to selectively alter the predetermined microdroplet characteristic or property using a feedback control.

公开(公告)号：US20200002748A1

公开(公告)日：2020-01-02

申请号：US16565773

申请日：2019-09-10

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Benjamin J. Miller ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6806 ,  B05B1/08 ,  B01F13/00 ,  B01F3/08 ,  B05B1/02 ,  B01L3/00

Abstract: The invention generally relates to methods and systems for manipulating droplet size. In certain aspects, the invention provides methods for manipulating droplet size that include forming droplets of aqueous fluid surrounded by an immiscible carrier fluid, and manipulating droplet size during the forming step by adjusting pressure exerted on the aqueous fluid or the carrier fluid.

公开(公告)号：US12241116B2

公开(公告)日：2025-03-04

申请号：US18536935

申请日：2023-12-12

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/68 ,  C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883 ,  C12Q1/6886

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US20240093271A1

公开(公告)日：2024-03-21

申请号：US18514645

申请日：2023-11-20

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

CPC classification number: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686 ,  C12Q1/6883

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US20230086845A1

公开(公告)日：2023-03-23

申请号：US17992304

申请日：2022-11-22

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jonathan William Larson ,  Qun Zhong ,  Darren Roy Link

IPC: C12Q1/6816 ,  C12Q1/6827 ,  C12Q1/686

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, methods of the invention involve forming sample droplets containing, on average, a single target nucleic acid, amplifying the target in the droplets, excluding droplets containing amplicon from the target and amplicon from a variant of the target, and analyzing target amplicons.

公开(公告)号：US10927407B2

公开(公告)日：2021-02-23

申请号：US16022360

申请日：2018-06-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link

IPC: B01L3/00 ,  C12Q1/6848 ,  G01N1/38 ,  C12Q1/686 ,  B01J19/00 ,  B01F13/00 ,  B03C5/00 ,  B03C5/02 ,  B01F3/08 ,  C12Q1/00 ,  C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6874 ,  G01N35/08 ,  G01N15/14 ,  C12N15/10 ,  C12Q1/6827 ,  B01L7/00 ,  B01L9/00 ,  G01N35/00

Abstract: The invention generally relates to assemblies for displacing droplets from a vessel that facilitate the collection and transfer of the droplets while minimizing sample loss. In certain aspects, the assembly includes at least one droplet formation module, in which the module is configured to form droplets surrounded by an immiscible fluid. The assembly also includes at least one chamber including an outlet, in which the chamber is configured to receive droplets and an immiscible fluid, and in which the outlet is configured to receive substantially only droplets. The assembly further includes a channel, configured such that the droplet formation module and the chamber are in fluid communication with each other via the channel. In other aspects, the assembly includes a plurality of hollow members, in which the hollow members are channels and in which the members are configured to interact with a vessel.

公开(公告)号：US20200318157A1

公开(公告)日：2020-10-08

申请号：US16838800

申请日：2020-04-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John Brian Hutchison ,  Jeffrey Charles Olson ,  Darren Roy Link

IPC: C12Q1/686

Abstract: The invention generally relates to droplet based digital PCR and methods for analyzing a target nucleic acid using the same. In certain embodiments, a method for determining the nucleic acid make-up of a sample is provided.

公开(公告)号：US12292440B2

公开(公告)日：2025-05-06

申请号：US18653506

申请日：2024-05-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren Roy Link

IPC: G01N33/543 ,  B01F33/3011 ,  B01F33/302 ,  C12Q1/6874 ,  C40B30/04 ,  C40B40/00 ,  C40B40/04 ,  C40B70/00 ,  G01N33/532

Abstract: The invention generally relates to performing sandwich assays in droplets. In certain embodiments, the invention provides methods for detecting a target analyte that involve forming a compartmentalized portion of fluid including a portion of a sample suspected of containing a target analyte and a sample identifier, a first binding agent having a target identifier, and a second binding agent specific to the target analyte under conditions that produce a complex of the first and second binding agents with the target analyte, separating the complexes, and detecting the complexes, thereby detecting the target analyte.