公开(公告)号：US20220033919A1

公开(公告)日：2022-02-03

申请号：US17502251

申请日：2021-10-15

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Darren R. Link ,  Sirshendu Roopom Banerjee

IPC: C12Q1/70

Abstract: The invention includes methods for determining the presence of a latent viral population by analyzing an RNA population from the virus with digital techniques, such as digital PCR or by sequencing cDNA produced from the RNA. The invention additional includes methods for determining the presence of latent viral populations by detecting and/or quantifying enzymes that are uniquely associated with the virus, e.g., reverse transcriptases.

公开(公告)号：US11168353B2

公开(公告)日：2021-11-09

申请号：US16400858

申请日：2019-05-01

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12Q1/6806 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/6804 ,  C12N15/10

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US10551382B2

公开(公告)日：2020-02-04

申请号：US16199822

申请日：2018-11-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Roy Link ,  Michael L. Samuels

IPC: G01N33/58 ,  G01N33/573 ,  B01F5/06 ,  B01J19/00 ,  B01F13/00 ,  B01L3/00 ,  B01F3/08 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  B01L7/00 ,  G01N21/64

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US11747327B2

公开(公告)日：2023-09-05

申请号：US16928924

申请日：2020-07-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: G01N33/53 ,  G01N33/532 ,  C40B50/08 ,  C12Q1/6874 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12N15/10

CPC classification number: G01N33/53 ,  C12N15/1075 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/532 ,  G01N33/5436 ,  G01N33/58 ,  C12Q2521/10 ,  C12Q2563/179 ,  C12Q2565/30 ,  G01N2458/10

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US20200225232A1

公开(公告)日：2020-07-16

申请号：US16733132

申请日：2020-01-02

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren R. Link ,  Michael L. Samuels

IPC: G01N33/573 ,  B01F5/06 ,  B01J19/00 ,  B01F13/00 ,  B01L3/00 ,  B01F3/08 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/58

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US10584332B2

公开(公告)日：2020-03-10

申请号：US14995744

申请日：2016-01-14

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12Q1/6874 ,  C12Q1/68 ,  C12P19/34 ,  C12N15/10 ,  C40B50/08 ,  G01N33/543 ,  G01N33/58

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US12140590B2

公开(公告)日：2024-11-12

申请号：US17529082

申请日：2021-11-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: G01N33/53 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/532 ,  G01N33/543 ,  G01N33/58

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US12038438B2

公开(公告)日：2024-07-16

申请号：US17509933

申请日：2021-10-25

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren R. Link ,  Michael L. Samuels

IPC: G01N33/573 ,  B01F23/41 ,  B01F25/433 ,  B01F33/3011 ,  B01J19/00 ,  B01L3/00 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6851 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  G01N33/542 ,  G01N33/58 ,  B01L7/00 ,  G01N21/64

CPC classification number: G01N33/573 ,  B01F23/41 ,  B01F25/433 ,  B01F25/4336 ,  B01F33/3011 ,  B01J19/0046 ,  B01L3/502761 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  C12Q1/6851 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/5008 ,  G01N33/542 ,  G01N33/582 ,  B01J2219/00286 ,  B01J2219/00351 ,  B01J2219/00418 ,  B01J2219/00479 ,  B01J2219/00576 ,  B01J2219/00585 ,  B01J2219/0059 ,  B01J2219/00592 ,  B01J2219/00599 ,  B01J2219/0065 ,  B01J2219/00657 ,  B01J2219/00664 ,  B01J2219/00702 ,  B01J2219/0072 ,  B01J2219/00722 ,  B01J2219/0074 ,  B01J2219/00743 ,  B01L7/52 ,  B01L2200/027 ,  B01L2300/0636 ,  B01L2300/0645 ,  B01L2300/0654 ,  B01L2300/0681 ,  B01L2300/0864 ,  B01L2300/0867 ,  B01L2400/0415 ,  B01L2400/0487 ,  G01N21/6428 ,  G01N21/6445 ,  G01N2500/00

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.

公开(公告)号：US20220064711A1

公开(公告)日：2022-03-03

申请号：US17529082

申请日：2021-11-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael L. Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: C12Q1/6806 ,  C40B50/08 ,  C12Q1/6874 ,  G01N33/543 ,  G01N33/58 ,  C12Q1/6804 ,  C12N15/10

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US20210088519A1

公开(公告)日：2021-03-25

申请号：US17081220

申请日：2020-10-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren R. Link ,  Michael L. Samuels

IPC: G01N33/573 ,  B01F5/06 ,  B01J19/00 ,  B01F13/00 ,  B01L3/00 ,  B01F3/08 ,  C40B40/04 ,  C40B50/08 ,  C40B60/10 ,  G01N33/50 ,  C12Q1/25 ,  C12Q1/6804 ,  C12Q1/6818 ,  C12Q1/6827 ,  G01N33/542 ,  G01N33/58

Abstract: The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.