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28 条记录 (耗时 0.038 秒)

    Chemical event selection by suicide substrate conjugates
    11.
    发明授权
    Chemical event selection by suicide substrate conjugates 失效
    化学事件选择由硅化物底物缀合物

    公开(公告)号：US5571681A

    公开(公告)日：1996-11-05

    申请号：US209525

    申请日：1994-03-10

    申请人： Kim D. Janda

    发明人： Kim D. Janda

    IPC分类号： C07K16/00 ,  G01N33/569 ,  C12Q1/25 ,  C12N9/00

    CPC分类号： C07K16/00 ,  G01N33/56983

    摘要： Molecules having covalent catalytic activity are identified by panning synthetic and semisynthetic combinatorial libraries on solid phase suicide substrates. In an alternative mode, mechanism-based inhibitors or affinity labels may substituted for the suicide substrate. Covalent catalysts within the combinatorial library form covalent conjugates with the suicide substrate, mechanism-based inhibitor, or affinity label. Covalent conjugates are immobilized by attachment to the suicide substrate to solid phase and are easily separated from unconjugated elements of the combinatorial library by stringent washing. Combinatorial libraries employing phagemid-display are particularly preferred since such phagemids include genetic material for identifying and amplifying conjugated catalysts. Covalent catalysts obtainable by this method include, inter alia, molecules having esterolytic activity, aldol condensation activity, .beta.-lactamase activity, glycosidase activity, RNase activity, and proteolytic activity.

    摘要翻译： 具有共价催化活性的分子通过在固相硅化物底物上平移合成和半合成组合文库来鉴定。 在替代模式中，基于机制的抑制剂或亲和标记可以代替硅化物底物。 组合文库内的共价催化剂与自杀底物，基于机制的抑制剂或亲和标记形成共价共轭体。 共价缀合物通过附着到硅化物底物固定而固定，并且通过严格洗涤容易地从组合文库的未缀合元件分离。 使用噬菌粒展示的组合库是特别优选的，因为这样的噬菌粒包括用于鉴定和扩增缀合的催化剂的遗传物质。 通过该方法可获得的共价催化剂尤其包括具有酯水解活性的分子，醛醇缩合活性，β-内酰胺酶活性，糖苷酶活性，核糖核酸酶活性和蛋白水解活性。

    Use of encoded reaction cassette
    20.
    发明授权
    Use of encoded reaction cassette 失效
    使用编码反应盒

    公开(公告)号：US06849398B1

    公开(公告)日：2005-02-01

    申请号：US08716448

    申请日：1996-01-18

    申请人： Kim D. Janda ,  Richard A. Lerner ,  Hicham Fenniri

    发明人： Kim D. Janda ,  Richard A. Lerner ,  Hicham Fenniri

    IPC分类号： C07H21/02 ,  C12M1/34 ,  C12P19/34 ,  C12Q1/68

    CPC分类号： C12Q1/686 ,  C12Q2563/131 ,  C12Q2521/301

    摘要： A reaction cassette has been designed for the highly sensitive detection of the making and breaking of chemical bonds. The system may be employed as a companion device to be used in the search for antibody and other novel catalysts. The cassette also has important clinical applications in the design of diagnostic reagents. In its fully encoded format this methodology is capable of both detecting and decoding chemical events.

    摘要翻译： 反应盒已被设计用于高灵敏度地检测化学键的制造和断裂。 该系统可以用作用于搜索抗体和其他新型催化剂的配套设备。 该盒式磁带在诊断试剂的设计中也具有重要的临床应用。 在其完全编码格式中，该方法能够检测和解码化学事件。