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    Anti-cocaine vaccine
    4.
    发明授权
    Anti-cocaine vaccine 失效
    抗可卡因疫苗

    公开(公告)号：US06383490B1

    公开(公告)日：2002-05-07

    申请号：US09077434

    申请日：1998-06-12

    申请人： Peter Wirsching ,  Kim D. Janda

    发明人： Peter Wirsching ,  Kim D. Janda

    IPC分类号： A61K39395

    CPC分类号： C07D451/04 ,  A61K31/46 ,  A61K39/0013 ,  A61K47/6803 ,  A61K2039/6081 ,  C07D451/12 ,  C07F9/6561 ,  C07K16/44 ,  Y10S530/807

    摘要： An anti-cocaine vaccine employs a cocaine hapten conjugated to a carrier protein. The anti-cocaine vaccine elicits an immune response which reduces the psychoactive effects of cocaine consumption by the production of anti-cocaine polyclonal antibodies. The antibodies may be employed in an ELISA test for assaying cocaine. The immune response elicited by the anti-cocaine vaccine produces antibody producing cells which may be isolated and cloned for producing anti-cocaine monoclonal antibodies.

    摘要翻译： 抗可卡因疫苗使用与载体蛋白缀合的可卡因半抗原。 抗可卡因疫苗引发免疫反应，通过生产抗可卡因多克隆抗体降低可卡因消费的精神影响。 抗体可用于测定可卡因的ELISA试验。 由抗可卡因疫苗引起的免疫应答产生抗体产生细胞，其可以被分离并克隆以产生抗可卡因单克隆抗体。

    Chemical event selection by suicide substrate conjugates
    5.
    发明授权
    Chemical event selection by suicide substrate conjugates 失效
    化学事件选择由硅化物底物缀合物

    公开(公告)号：US5571681A

    公开(公告)日：1996-11-05

    申请号：US209525

    申请日：1994-03-10

    申请人： Kim D. Janda

    发明人： Kim D. Janda

    IPC分类号： C07K16/00 ,  G01N33/569 ,  C12Q1/25 ,  C12N9/00

    CPC分类号： C07K16/00 ,  G01N33/56983

    摘要： Molecules having covalent catalytic activity are identified by panning synthetic and semisynthetic combinatorial libraries on solid phase suicide substrates. In an alternative mode, mechanism-based inhibitors or affinity labels may substituted for the suicide substrate. Covalent catalysts within the combinatorial library form covalent conjugates with the suicide substrate, mechanism-based inhibitor, or affinity label. Covalent conjugates are immobilized by attachment to the suicide substrate to solid phase and are easily separated from unconjugated elements of the combinatorial library by stringent washing. Combinatorial libraries employing phagemid-display are particularly preferred since such phagemids include genetic material for identifying and amplifying conjugated catalysts. Covalent catalysts obtainable by this method include, inter alia, molecules having esterolytic activity, aldol condensation activity, .beta.-lactamase activity, glycosidase activity, RNase activity, and proteolytic activity.

    摘要翻译： 具有共价催化活性的分子通过在固相硅化物底物上平移合成和半合成组合文库来鉴定。 在替代模式中，基于机制的抑制剂或亲和标记可以代替硅化物底物。 组合文库内的共价催化剂与自杀底物，基于机制的抑制剂或亲和标记形成共价共轭体。 共价缀合物通过附着到硅化物底物固定而固定，并且通过严格洗涤容易地从组合文库的未缀合元件分离。 使用噬菌粒展示的组合库是特别优选的，因为这样的噬菌粒包括用于鉴定和扩增缀合的催化剂的遗传物质。 通过该方法可获得的共价催化剂尤其包括具有酯水解活性的分子，醛醇缩合活性，β-内酰胺酶活性，糖苷酶活性，核糖核酸酶活性和蛋白水解活性。