利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

48 条记录 (耗时 0.035 秒)

    Method for producing methionine using a microorganism with reduced expression activity of a negative regulator of methionine biosynthesis
    16.
    发明授权
    Method for producing methionine using a microorganism with reduced expression activity of a negative regulator of methionine biosynthesis 有权
    使用具有降低甲硫氨酸生物合成负调节子的表达活性的微生物生产甲硫氨酸的方法

    公开(公告)号：US08389251B2

    公开(公告)日：2013-03-05

    申请号：US13018119

    申请日：2011-01-31

    申请人： Markus Pompejus ,  Burkhard Kröger ,  Hartwig Schröder ,  Oskar Zelder ,  Gregor Haberhauer ,  Stefan Haefner ,  Corrinna Klopprogge

    发明人： Markus Pompejus ,  Burkhard Kröger ,  Hartwig Schröder ,  Oskar Zelder ,  Gregor Haberhauer ,  Stefan Haefner ,  Corrinna Klopprogge

    IPC分类号： C12P13/12 ,  C12N15/74

    CPC分类号： C07K14/34

    摘要： Isolated nucleic acid molecules, designated MR nucleic acid molecules, which encode novel MR proteins from Corynebacterium glutamicum are described which are involved in biosynthesis of a fine chemical, e.g., methionine biosynthesis. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing MR nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides methods of producing methionine from microorganisms, e.g., C. glutamicum, which involve culturing recombinant microorganisms which overexpress or underexpress at least one MR molecule of the invention under conditions such that methionine is produced. Also featured are methods of producing a fine chemical, e.g., methionine, which involve culturing recombinant microorganisms having selected MR genes deleted or mutated under conditions such that the fine chemical, e.g., methionine, is produced.

    摘要翻译： 描述了编码来自谷氨酸棒杆菌的新型MR蛋白质的分离的核酸分子，称为MR核酸分子，其涉及精细化学品的生物合成，例如甲硫氨酸生物合成。 本发明还提供反义核酸分子，含有MR核酸分子的重组表达载体和已经引入表达载体的宿主细胞。 本发明还提供了从微生物例如谷氨酸棒杆菌制备甲硫氨酸的方法，其涉及在产生甲硫氨酸的条件下培养过表达或低表达本发明的至少一种MR分子的重组微生物。 还特征在于生产精细化学品的方法，例如甲硫氨酸，其涉及在产生精细化学物质例如甲硫氨酸的条件下培养具有选择的MR基因缺失或突变的重组微生物。

    CORYNEBACTERIUM GLUTAMICUM GENES ENCODING REGULATORY PROTEINS INVOLVED IN THE PRODUCTION OF METHIONINE
    17.
    发明申请
    CORYNEBACTERIUM GLUTAMICUM GENES ENCODING REGULATORY PROTEINS INVOLVED IN THE PRODUCTION OF METHIONINE 有权
    CORYNEBACTERIUM GLUTAMICUM GENES编码参与甲磺酸生产的法规蛋白质

    公开(公告)号：US20110229937A1

    公开(公告)日：2011-09-22

    申请号：US13018119

    申请日：2011-01-31

    申请人： Markus Pompejus ,  Burkhard Kröger ,  Hartwig Schröder ,  Oskar Zelder ,  Gregor Haberhauer ,  Stefan Haefner ,  Corrinna Klopprogge

    发明人： Markus Pompejus ,  Burkhard Kröger ,  Hartwig Schröder ,  Oskar Zelder ,  Gregor Haberhauer ,  Stefan Haefner ,  Corrinna Klopprogge

    IPC分类号： C12P19/40 ,  C12P13/12 ,  C12N15/63 ,  C12P13/04

    CPC分类号： C07K14/34

    摘要： Isolated nucleic acid molecules, designated MR nucleic acid molecules, which encode novel MR proteins from Corynebacterium glutamicum are described which are involved in biosynthesis of a fine chemical, e.g., methionine biosynthesis. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing MR nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides methods of producing methionine from microorganisms, e.g., C. glutamicum, which involve culturing recombinant microorganisms which overexpress or underexpress at least one MR molecule of the invention under conditions such that methionine is produced. Also featured are methods of producing a fine chemical, e.g., methionine, which involve culturing recombinant microorganisms having selected MR genes deleted or mutated under conditions such that the fine chemical, e.g., methionine, is produced.

    摘要翻译： 描述了编码来自谷氨酸棒杆菌的新型MR蛋白质的分离的核酸分子，称为MR核酸分子，其涉及精细化学品的生物合成，例如甲硫氨酸生物合成。 本发明还提供反义核酸分子，含有MR核酸分子的重组表达载体和已经引入表达载体的宿主细胞。 本发明还提供了从微生物例如谷氨酸棒杆菌制备甲硫氨酸的方法，其涉及在产生甲硫氨酸的条件下培养过表达或低表达本发明的至少一种MR分子的重组微生物。 还特征在于生产精细化学品的方法，例如甲硫氨酸，其涉及在产生精细化学物质例如甲硫氨酸的条件下培养具有选择的MR基因缺失或突变的重组微生物。

    Nucleic acid molecules encoding metabolic regulatory proteins from Corynebacterium glutamicum, useful for increasing the production of methionone by a microorganism
    18.
    发明授权
    Nucleic acid molecules encoding metabolic regulatory proteins from Corynebacterium glutamicum, useful for increasing the production of methionone by a microorganism 失效
    编码来自谷氨酸棒杆菌的代谢调节蛋白的核酸分子，用于增加微生物产生的甲硫氨酸

    公开(公告)号：US07892798B2

    公开(公告)日：2011-02-22

    申请号：US10307138

    申请日：2002-11-29

    申请人： Markus Pompejus ,  Burkhard Kröger ,  Hartwig Schröder ,  Oskar Zelder ,  Gregor Haberhauer ,  Stefan Haefner ,  Corrinna Klopprogge

    发明人： Markus Pompejus ,  Burkhard Kröger ,  Hartwig Schröder ,  Oskar Zelder ,  Gregor Haberhauer ,  Stefan Haefner ,  Corrinna Klopprogge

    IPC分类号： C12P13/12 ,  C12P21/06 ,  C12N1/20 ,  C12N15/00 ,  C07H21/04

    CPC分类号： C07K14/34

    摘要： Isolated nucleic acid molecules, which encode novel metabolic regulatory proteins from Corynebacterium glutamicum are described. These nucleic acid molecules are involved in the biosynthesis of a fine chemical, e.g., methionine biosynthesis. The invention also provides antisense nucleic acid molecules, recombinant expression vectors containing metabolic regulatory nucleic acid molecules, and host cells into which the expression vectors have been introduced. The invention still further provides methods of producing methionine from microorganisms, e.g., C. glutamicum, which involve culturing recombinant microorganisms which overexpress or underexpress at least one metabolic regulatory molecule of the invention under conditions such that methionine is produced. Also featured are methods of producing a fine chemical, e.g., methionine, which involve culturing recombinant microorganisms having selected metabolic regulatory genes deleted or mutated under conditions such that the fine chemical, e.g., methionine, is produced.

    摘要翻译： 描述了编码来自谷氨酸棒杆菌的新型代谢调节蛋白的分离的核酸分子。 这些核酸分子参与精细化学品的生物合成，例如甲硫氨酸生物合成。 本发明还提供了反义核酸分子，含有代谢调控核酸分子的重组表达载体，以及引入了表达载体的宿主细胞。 本发明还提供了从微生物例如谷氨酸棒杆菌制备甲硫氨酸的方法，其涉及在产生甲硫氨酸的条件下培养过表达或低表达本发明的至少一种代谢调节分子的重组微生物。 还特征在于生产精细化学品的方法，例如甲硫氨酸，其涉及培养在产生精细化学物质（例如甲硫氨酸）的条件下缺失或突变的选择的代谢调节基因的重组微生物。

    Use of dimethyl disulfide for methionine production in microoragnisms
    19.
    发明申请
    Use of dimethyl disulfide for methionine production in microoragnisms 有权
    二甲基二硫化物用于微生物中的甲硫氨酸生产

    公开(公告)号：US20090281353A1

    公开(公告)日：2009-11-12

    申请号：US11988971

    申请日：2006-07-18

    申请人： Oskar Zelder ,  Stefan Haefner ,  Andrea Herold ,  Corinna Klopprogge ,  Hartwig Schroder ,  R. Rogers Yocum ,  Mark K. Williams

    发明人： Oskar Zelder ,  Stefan Haefner ,  Andrea Herold ,  Corinna Klopprogge ,  Hartwig Schroder ,  R. Rogers Yocum ,  Mark K. Williams

    IPC分类号： C12P13/12 ,  C07C323/03 ,  C12N1/21 ,  C12N15/31 ,  C12Q1/68 ,  C12N9/00

    CPC分类号： C12P13/12

    摘要： The present invention features improved processes and organisms for the production of methionine. The invention demonstrates that a ΔmetF organism or a ΔmetE AmetH organism, for example, mutants of C. glutamicum or E. coli, can use a methyl capped sulfide source, e.g., dimethyl disulfide (DMDS), as a source of both sulfur and a methyl group, bypassing the need for MetH/MetE and MetF activity and the need to reduce sulfate, for the synthesis of methionine. Also described in this patent are data implicating MetY (also called MetZ) as an enzyme that incorporates a methyl capped sulfide source, e.g., DMDS, into methionine. A ΔmetF ΔmetB strain of C. glutamicum can use a methyl capped sulfide source, e.g., DMDS, as a source of both sulfide and a methyl group. Furthermore, methionine production by engineered prototrophic organisms that overproduce O-acetyl-homoserine was improved by the addition of a methyl capped sulfide source, e.g., DMDS.

    摘要翻译： 本发明的特征在于用于生产甲硫氨酸的改进方法和生物体。 本发明证明，DeltametF生物体或DeltametE AmetH生物体，例如谷氨酸棒状杆菌或大肠杆菌的突变体可以使用甲基封端的硫化物源，例如二甲基二硫醚（DMDS）作为硫和 甲基，绕过MetH / MetE和MetF活性的需要，需要还原硫酸盐，用于合成甲硫氨酸。 该专利中还描述的是涉及MetY（也称为MetZ）作为将甲基封端的硫化物源例如DMDS掺入甲硫氨酸的酶的数据。 谷氨酸棒状病毒的DeltametF DeltametB菌株可以使用甲基封端的硫化物源，例如DMDS，作为硫化物和甲基两者的来源。 此外，通过加入甲基封端的硫化物源（例如DMDS），改善过量产生O-乙酰高丝氨酸的工程化原养殖生物体的甲硫氨酸生产。