公开(公告)号：US08252555B2

公开(公告)日：2012-08-28

申请号：US12521141

申请日：2007-12-21

申请人： Oskar Zelder ,  Wolfgang Grabarse ,  Corinna Klopprogge ,  Hartwig Schröder ,  Stefan Haefner ,  Anja Knietsch ,  Andrea Herold

发明人： Oskar Zelder ,  Wolfgang Grabarse ,  Corinna Klopprogge ,  Hartwig Schröder ,  Stefan Haefner ,  Anja Knietsch ,  Andrea Herold

IPC分类号： C12P21/06

CPC分类号： C12N9/0091 ,  C12P13/12

摘要： The present invention relates to nucleotide sequences encoding enzymatically active cobalamin-methionine synthase and functional fragments thereof that are modified in comparison to the respective wild-type enzyme such that said enzymes show reduced product inhibition by methionine. The present invention also relates to polypeptides being encoded by such nucleotide sequences and host cells comprising such nucleotide sequences. Furthermore, the present invention relates to methods for producing methionine in host organisms by making use of such nucleotide sequences.

摘要翻译： 本发明涉及编码酶促活性钴胺素 - 甲硫氨酸合成酶的核苷酸序列及其功能片段，其与相应的野生型酶相比被修饰，使得所述酶显示产品对甲硫氨酸的抑制作用降低。 本发明还涉及由该核苷酸序列编码的多肽和包含该核苷酸序列的宿主细胞。 此外，本发明涉及通过利用这种核苷酸序列在宿主生物体中产生甲硫氨酸的方法。

公开(公告)号：US20100035307A1

公开(公告)日：2010-02-11

申请号：US12521141

申请日：2007-12-21

申请人： Oskar Zelder ,  Wolfgang Grabarse ,  Corinna Klopprogge ,  Hartwig Schröder ,  Stefan Haefner ,  Anja Knietsch ,  Andrea Herold

发明人： Oskar Zelder ,  Wolfgang Grabarse ,  Corinna Klopprogge ,  Hartwig Schröder ,  Stefan Haefner ,  Anja Knietsch ,  Andrea Herold

IPC分类号： C12P13/12 ,  C07H21/04 ,  C12N15/63 ,  C12N1/21

CPC分类号： C12N9/0091 ,  C12P13/12

摘要： The present invention relates to nulceotide sequences encoding enzymatically active cobalamin-methionine synthase and functional fragments thereof being modified in comparison to the respective wild-type enzyme such that said enzymes show reduced product inhibition by methionine. The present invention also relates to polypeptides being encoded by such nucleotide sequences and host cells comprising such nucleotide sequences. Furthermore, the present invention relates to methods for producing methionine in host organisms by making use of such nucleotide sequences.

摘要翻译： 本发明涉及编码酶促活性钴胺素 - 甲硫氨酸合成酶的核苷酸序列及其功能片段，其与相应的野生型酶相比被修饰，使得所述酶显示产品抑制蛋氨酸的产物减少。 本发明还涉及由该核苷酸序列编码的多肽和包含该核苷酸序列的宿主细胞。 此外，本发明涉及通过利用这种核苷酸序列在宿主生物体中产生甲硫氨酸的方法。

公开(公告)号：US20120295315A1

公开(公告)日：2012-11-22

申请号：US13524184

申请日：2012-06-15

申请人： Oskar Zelder ,  Wolfgang Grabarse ,  Corinna Klopprogge ,  Hartwig Schröder ,  Stefan Haefner ,  Anja Knietsch ,  Andrea Herold

发明人： Oskar Zelder ,  Wolfgang Grabarse ,  Corinna Klopprogge ,  Hartwig Schröder ,  Stefan Haefner ,  Anja Knietsch ,  Andrea Herold

IPC分类号： C12N15/54 ,  C12N1/21 ,  C12P13/12 ,  C12N15/63

CPC分类号： C12N9/0091 ,  C12P13/12

摘要： The present invention relates to nulceotide sequences encoding enzymatically active cobalamin-methionine synthase and functional fragments thereof being modified in comparison to the respective wild-type enzyme such that said enzymes show reduced product inhibition by methionine. The present invention also relates to polypeptides being encoded by such nucleotide sequences and host cells comprising such nucleotide sequences. Furthermore, the present invention relates to methods for producing methionine in host organisms by making use of such nucleotide sequences.

摘要翻译： 本发明涉及编码酶促活性钴胺素 - 甲硫氨酸合成酶的核糖核酸序列及其功能片段，其与相应的野生型酶相比被修饰，使得所述酶显示产品抑制蛋氨酸的产物减少。 本发明还涉及由该核苷酸序列编码的多肽和包含该核苷酸序列的宿主细胞。 此外，本发明涉及通过利用这种核苷酸序列在宿主生物体中产生甲硫氨酸的方法。

公开(公告)号：US20120149617A1

公开(公告)日：2012-06-14

申请号：US13313400

申请日：2011-12-07

申请人： Arno LANGE ,  Harald Böhnke ,  Wolfgang Grabarse ,  Hannah Maria König ,  Markus Hansch ,  Ludwig Völkel ,  Ivette Garcia Castro

发明人： Arno LANGE ,  Harald Böhnke ,  Wolfgang Grabarse ,  Hannah Maria König ,  Markus Hansch ,  Ludwig Völkel ,  Ivette Garcia Castro

IPC分类号： C10L1/233 ,  C10M169/04 ,  C07D265/14

CPC分类号： C10L10/04 ,  C07D265/16 ,  C10L1/233 ,  C10L1/238 ,  C10L2200/0259 ,  C10L2230/22 ,  C10L2270/026 ,  C10M133/48 ,  C10M149/14 ,  C10M2215/225 ,  C10M2217/041 ,  C10N2220/021

摘要： Polytetrahydrobenzoxazines and bistetrahydrobenzoxazines, obtainable by (A) reacting at least one diamine of the formula H2N-A-NH2 with a C1- to C12-aldehyde and a C1- to C8-alkanol at 20 to 80° C. with elimination and removal of water, (B) reacting the condensation product from (A) with a phenol which bears a long-chain substituent at 30 to 120° C., and optionally (C) heating the reaction product from (B) to 125 to 280° C. The resulting polytetrahydrobenzoxazines and bistetrahydrobenzoxazines are suitable as fuel or lubricant additives, especially as detergent additives for diesel fuels.

摘要翻译： 可通过（A）使式H2N-A-NH2的至少一种二胺与C 1 -C 12 - 醛和C 1 -C 8烷醇在20至80℃下反应得到的聚四氢苯并恶嗪和双四氢苯并恶嗪，同时除去和除去 水，（B）使来自（A）的缩合物与长链取代基的苯酚在30〜120℃下反应，任选（C）将反应产物从（B）加热至125〜280℃ 所得聚四氢苯并恶嗪和双四氢苯并恶嗪适用作燃料或润滑剂添加剂，特别是作为柴油燃料的洗涤剂添加剂。

公开(公告)号：US20110258917A1

公开(公告)日：2011-10-27

申请号：US13093247

申请日：2011-04-25

申请人： Ivette Garcia Castro ,  Cornelia Roeger-Goepfert ,  Wolfgang Grabarse ,  Klaus Mühlbach ,  Ludwig Völkel ,  Harald Böhnke ,  Christian Tock

发明人： Ivette Garcia Castro ,  Cornelia Roeger-Goepfert ,  Wolfgang Grabarse ,  Klaus Mühlbach ,  Ludwig Völkel ,  Harald Böhnke ,  Christian Tock

IPC分类号： C10L1/18 ,  C08F26/02

CPC分类号： C10L1/221 ,  C08F8/44 ,  C08F210/02 ,  C08F2800/10 ,  C08F2810/50 ,  C10L1/1641 ,  C10L1/1963 ,  C10L1/2366 ,  C10L1/2368 ,  C10L10/08 ,  C10L2200/0259 ,  C10L2230/22 ,  C10L2270/026 ,  C08F218/08 ,  C08F220/34 ,  C08F8/02 ,  C08F2500/02

摘要： Quaternized terpolymer formed from (A) ethylene, (B) C2-C14-alkenyl esters of one or more aliphatic C1-C20-monocarboxylic acids or C1-C24-alkyl esters of acrylic acid or of methacrylic acid and (Cq) ethylenically unsaturated monomers which comprise at least one tertiary nitrogen atom which is partly or fully in quaternized form. The quaternized terpolymer is suitable as a fuel additive for reducing or preventing deposits, for reducing fuel consumption and for minimizing power loss in direct-injection diesel engines, especially in diesel engines with common-rail injection systems.

摘要翻译： 由（A）乙烯，（B）一种或多种脂族C 1 -C 20 - 一元羧酸或丙烯酸或甲基丙烯酸和（Cq）烯属不饱和单体的C 1 -C 24烷基酯形成的季铵化三元共聚物 其包含部分或全部为季铵化形式的至少一个叔氮原子。 季铵化三元共聚物适合用作减少或防止沉积物的燃料添加剂，用于降低燃料消耗和最小化直喷式柴油发动机，特别是在具有共轨喷射系统的柴油发动机中的功率损耗。

公开(公告)号：US20110160594A1

公开(公告)日：2011-06-30

申请号：US13061767

申请日：2009-09-04

申请人： Herbert Platsch ,  Helmut Schiffer ,  Rolf Hellmann ,  Stefan Emig ,  Wolfgang Grabarse ,  Harald Koehle ,  Klaus Grossmann ,  Juergen Langewald ,  Christine Boeddinghaus ,  John Dorsch

发明人： Herbert Platsch ,  Helmut Schiffer ,  Rolf Hellmann ,  Stefan Emig ,  Wolfgang Grabarse ,  Harald Koehle ,  Klaus Grossmann ,  Juergen Langewald ,  Christine Boeddinghaus ,  John Dorsch

IPC分类号： A61B6/00 ,  C12Q1/02

CPC分类号： G01N33/5082

摘要： The present invention relates to a method for testing a substance or a substance mixture and the use thereof for identifying and/or characterizing the mode of action of the substance or the substance mixture or for determining the biochemical and/or metabolic state of an organism or group of organisms or part(s) thereof after exposure to the substance or the substance mixture.

摘要翻译： 本发明涉及用于测定物质或物质混合物的方法及其用于鉴定和/或表征物质或物质混合物的作用方式或用于确定生物体的生化和/或代谢状态的用途， 一组生物体或其部分暴露于物质或物质混合物后。

公开(公告)号：US09006158B2

公开(公告)日：2015-04-14

申请号：US13313400

申请日：2011-12-07

申请人： Arno Lange ,  Harald Böhnke ,  Wolfgang Grabarse ,  Hannah Maria König ,  Markus Hansch ,  Ludwig Völkel ,  Ivette Garcia Castro

发明人： Arno Lange ,  Harald Böhnke ,  Wolfgang Grabarse ,  Hannah Maria König ,  Markus Hansch ,  Ludwig Völkel ,  Ivette Garcia Castro

IPC分类号： C10L1/233 ,  C10M169/04 ,  C07D265/14 ,  C07D265/16 ,  C10L1/238 ,  C10L10/04 ,  C10M133/48 ,  C10M149/14

CPC分类号： C10L10/04 ,  C07D265/16 ,  C10L1/233 ,  C10L1/238 ,  C10L2200/0259 ,  C10L2230/22 ,  C10L2270/026 ,  C10M133/48 ,  C10M149/14 ,  C10M2215/225 ,  C10M2217/041 ,  C10N2220/021

摘要： Polytetrahydrobenzoxazines and bistetrahydrobenzoxazines, obtainable by (A) reacting at least one diamine of the formula H2N-A-NH2 with a C1- to C12-aldehyde and a C1- to C8-alkanol at 20 to 80° C. with elimination and removal of water, (B) reacting the condensation product from (A) with a phenol which bears a long-chain substituent at 30 to 120° C., and optionally (C) heating the reaction product from (B) to 125 to 280° C. The resulting polytetrahydrobenzoxazines and bistetrahydrobenzoxazines are suitable as fuel or lubricant additives, especially as detergent additives for diesel fuels.

摘要翻译： 可通过（A）使式H2N-A-NH2的至少一种二胺与C 1 -C 12 - 醛和C 1 -C 8烷醇在20至80℃下反应得到的聚四氢苯并恶嗪和双四氢苯并恶嗪，同时除去和除去 水，（B）使来自（A）的缩合物与长链取代基的苯酚在30〜120℃下反应，任选（C）将反应产物从（B）加热至125〜280℃ 所得聚四氢苯并恶嗪和双四氢苯并恶嗪适用作燃料或润滑剂添加剂，特别是作为柴油燃料的洗涤剂添加剂。

公开(公告)号：US20130133243A1

公开(公告)日：2013-05-30

申请号：US13535847

申请日：2012-06-28

申请人： Cornelia Röger-Göpfert ,  Harald Böhnke ,  Wolfgang Grabarse ,  Hannah Maria König ,  Markus Hansch ,  Ludwig Völkel

发明人： Cornelia Röger-Göpfert ,  Harald Böhnke ,  Wolfgang Grabarse ,  Hannah Maria König ,  Markus Hansch ,  Ludwig Völkel

IPC分类号： C10L1/22

CPC分类号： C10L1/221 ,  C10L1/2383 ,  C10L10/04 ,  C10L10/06 ,  C10L10/14 ,  C10L2200/0259 ,  C10L2270/023 ,  C10L2270/026 ,  C10M133/58 ,  C10M2215/28 ,  C10N2230/04 ,  C10N2270/00 ,  C10N2260/00

摘要： The present invention relates to novel quaternized nitrogen compounds, to the preparation thereof and to the use thereof as a fuel and lubricant additive, more particularly as a detergent additive; to additive packages which comprise these compounds; and to fuels and lubricants thus additized. The present invention further relates to the use of these quaternized nitrogen compounds as a fuel additive for reducing or preventing deposits in the injection systems of direct-injection diesel engines, especially in common-rail injection systems, for reducing the fuel consumption of direct-injection diesel engines, especially of diesel engines with common-rail injection systems, and for minimizing power loss in direct-injection diesel engines, especially in diesel engines with common-rail injection systems.

公开(公告)号：US08911516B2

公开(公告)日：2014-12-16

申请号：US13168405

申请日：2011-06-24

申请人： Wolfgang Grabarse ,  Hannah Maria Koenig ,  Christian Tock ,  Cornelia Roeger-Goepfert ,  Ludwig Voelkel ,  Harald Boehnke

发明人： Wolfgang Grabarse ,  Hannah Maria Koenig ,  Christian Tock ,  Cornelia Roeger-Goepfert ,  Ludwig Voelkel ,  Harald Boehnke

IPC分类号： C10L1/22 ,  C08F222/06 ,  C10L1/236 ,  C10L10/08 ,  C10L10/18 ,  C10L10/04

CPC分类号： C08F222/06 ,  C08F8/44 ,  C10L1/2364 ,  C10L1/2366 ,  C10L10/04 ,  C10L10/08 ,  C10L10/18 ,  C08F220/14 ,  C08F8/32 ,  C08F8/14

摘要： A quaternized copolymer obtainable by: (A) copolymerization of straight-chain, branched or cyclic, ethylenically unsaturated C2 to C100 hydrocarbons, which may bear oxygen- or nitrogen-functional substituents which cannot be reacted with amines to give amides or imides or with alcohols to give esters, with ethylenically unsaturated C3- to C12-mono- or dicarboxylic acids or acid derivatives, which can be reacted with amines to give amides or imides or with alcohols to give esters, to give a copolymer with a number-average molecular weight of 500 to 20 000; (B) partial or full amidation or imidation or esterification of the carboxylic acid functions by reacting them with oligoamines having 2 to 6 nitrogen atoms or alcoholamines, each of which comprises primary or secondary nitrogen atom or a hydroxyl group and a quaternizable tertiary nitrogen atom; (C) partial or full quaternization of the tertiary nitrogen atom with a quaternizing agent. The quaternized copolymer thus prepared is suitable as a fuel additive in direct-injection diesel engines.

摘要翻译： 可以通过以下方法获得的季铵化共聚物：（A）直链，支链或环状的烯属不饱和C2至C100烃的共聚，其可承载不能与胺反应得到酰胺或酰亚胺或与醇反应的氧或氮官能取代基 得到具有烯键式不饱和C 3 -C 12 - 单 - 或二羧酸或酸衍生物的酯，其可以与胺反应得到酰胺或酰亚胺或与醇反应得到酯，得到具有数均分子量的共聚物 500至20,000; （B）通过使羧酸官能团与具有2-6个氮原子的低聚胺或醇胺进行部分或完全酰化或酰化或酯化，其各自包含伯或仲氮原子或羟基和可季铵化的叔氮原子; （C）叔氮原子与季铵化剂部分或全部季铵化。 如此制备的季铵化共聚物适合作为直接喷射柴油发动机中的燃料添加剂。

公开(公告)号：US20120010112A1

公开(公告)日：2012-01-12

申请号：US13177042

申请日：2011-07-06

申请人： Wolfgang Grabarse ,  Harald Böhnke ,  Christian Tock ,  Cornelia Röger-Göpfert ,  Ludwig Völkel

发明人： Wolfgang Grabarse ,  Harald Böhnke ,  Christian Tock ,  Cornelia Röger-Göpfert ,  Ludwig Völkel

IPC分类号： C10M169/04 ,  C07C237/20 ,  C07C231/02 ,  C10L1/222 ,  C10L1/232

CPC分类号： C10L10/04 ,  C07C231/02 ,  C07C231/12 ,  C07D207/12 ,  C10L1/22 ,  C10L1/2222 ,  C10L1/224 ,  C10L1/232 ,  C10L1/2383 ,  C10L1/2387 ,  C10L10/00 ,  C10L10/14 ,  C10L10/18 ,  C10L2200/0259 ,  C10L2230/22 ,  C10L2270/026 ,  C10M133/16 ,  C10M133/44 ,  C10M133/56 ,  C10M2203/1045 ,  C10M2215/082 ,  C10M2215/223 ,  C10M2215/28 ,  C10N2220/021 ,  C10N2230/04 ,  C10N2240/10

摘要： The present invention relates to novel acid-free quaternized nitrogen compounds, to the preparation thereof and to the use thereof as a fuel and lubricant additive, more particularly as a detergent additive, as a wax antisettling additive (WASA) or as an additive for reducing internal diesel injector deposits (IDID); to additive packages which comprise these compounds; and to fuels and lubricants thus additized. The present invention further relates to the use of these acid-free quaternized nitrogen compounds as a fuel additive for reducing or preventing deposits in the injection systems of direct-injection diesel engines, especially in common-rail injection systems, for reducing the fuel consumption of direct-injection diesel engines, especially of diesel engines with common-rail injection systems, and for minimizing power loss in direct-injection diesel engines, especially in diesel engines with common-rail injection systems.

摘要翻译： 本发明涉及新型无酸季铵化氮化合物，其制备方法及其作为燃料和润滑剂添加剂，更特别地作为洗涤剂添加剂，作为蜡抗沉降添加剂（WASA）或用作还原剂的添加剂 内部柴油喷射器沉积物（IDID）; 包括这些化合物的添加剂包; 并因此增加燃料和润滑剂。 本发明还涉及这些无酸季铵化氮化合物作为燃料添加剂用于减少或防止直喷式柴油发动机，特别是共轨喷射系统的喷射系统中的沉积的用途，用于降低燃料消耗 直喷柴油发动机，特别是具有共轨喷射系统的柴油发动机，并且用于最小化直喷式柴油发动机，特别是具有共轨喷射系统的柴油发动机中的功率损耗。