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公开(公告)号：US20240358765A1
公开(公告)日：2024-10-31
申请号：US18767576
申请日：2024-07-09
Applicant: Terumo Kabushiki Kaisha , Osaka University
Inventor： Hidetoshi EGUCHI , Yoshito TOMIMARU , Shigeru MIYAGAWA , Keisuke TOYA , Fumiya OHASHI
CPC classification number: A61K35/34 , A61P1/16 , C12N5/0658 , C12N2502/28
Abstract: A composition comprising skeletal myoblasts for treating liver dysfunction or improving liver function, in which the liver dysfunction can be hepatitis, liver fibrosis, liver cirrhosis, liver cancer, or liver failure. The composition can be used for promoting the proliferation of liver cells, regeneration of the liver tissue and/or angiogenesis, and providing a method for treating liver dysfunction, including a step of treating applying the composition to a site exhibiting liver dysfunction.
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12.发明公开公开(公告)号：US20240316115A1
公开(公告)日：2024-09-26
申请号：US18579093
申请日：2022-07-14
Applicant: Astellas Pharma Inc. , OSAKA UNIVERSITY
Inventor： Kenichiro SHIMATANI , Hiromu SATO , Masao SASAI , Yoshiki SAWA , Atsuhiro SAITO , Shigeru MIYAGAWA
CPC classification number: A61K35/44 , C12N5/069 , C12N2500/32 , C12N2500/90 , C12N2501/115 , C12N2501/135 , C12N2506/02 , C12N2509/00 , C12N2513/00 , C12N2533/52 , C12N2533/54
Abstract: An object of the present invention is to provide a pericyte-like cell having high angiogenic potential with a higher cell proliferation ability than a primary pericyte available in the past and high VEGF expression, and a method for producing the same. Provided are a method for producing a VEGF-highly expressing pericyte-like cell, the method including selecting a CD56(−) pericyte-like cell from a population including a pericyte-like cell obtained by inducing differentiation of a pluripotent stem cell; and a VEGF-highly expressing pericyte-like cell produced by the production method.
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公开(公告)号：US20220249613A1
公开(公告)日：2022-08-11
申请号：US17734550
申请日：2022-05-02
Applicant: OSAKA UNIVERSITY , TERUMO KABUSHIKI KAISHA
Inventor： Yoshiki SAWA , Shigeru MIYAGAWA , Keisuke MIYAKE , Kenji OYAMA
Abstract: Cell cultures for treating peripheral arterial disease, methods for producing the cell cultures and methods for treating peripheral arterial disease using the cell cultures are provided. The cell cultures may be 100 to 500 μm in size and may possess an extracellular matrix on an outer surface of the cell culture, and the like are provided.
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公开(公告)号：US20210187031A1
公开(公告)日：2021-06-24
申请号：US16071123
申请日：2017-01-18
Applicant: OSAKA UNIVERSITY
Inventor： Daisuke KAJITA , Satsuki FUKUSHIMA , Shigeru MIYAGAWA , Yoshiki SAWA
IPC: A61K35/28 , A61K35/35 , A61K35/545 , A61K35/51
Abstract: It has been found that a cell cluster obtained by causing just isolated cells to adhere to each other secretes adiponectin after transplantation to the heart, and thereby has an excellent therapeutic effect on heart disease.
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公开(公告)号：US20210009934A1
公开(公告)日：2021-01-14
申请号：US17042301
申请日：2019-03-12
Applicant: DAI NIPPON PRINTING CO., LTD. , OSAKA UNIVERSITY , TERUMO KABUSHIKI KAISHA
Inventor： Taro NAGAI , Yoshiki SAWA , Shigeru MIYAGAWA , Kenji OYAMA
Abstract: When detachment of a cell sheet from a culture dish has started at an unintended timing, immediately detach the cell sheet from the culture dish while preventing generation of wrinkles and the like in the cell sheet. A cell culture device captures an image of cells in the culture dish, and controls a cooling mechanism and a shaking mechanism based on the image so as to shake the cells while cooling them.
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Patent Agency Ranking
公开(公告)号：US20250082690A1
公开(公告)日：2025-03-13
申请号：US18767567
申请日：2024-07-09
Applicant: TERUMO KABUSHIKI KAISHA , OSAKA UNIVERSITY
Inventor： Hidetoshi EGUCHI , Yoshito TOMIMARU , Shigeru MIYAGAWA , Keisuke TOYA , Fumiya OHASHI
Abstract: A sheet-shaped cell culture comprising skeletal myoblasts for treating liver dysfunction or improving liver function. A method for producing the sheet-shaped cell culture, including a step of seeding a cell population comprising skeletal myoblasts on a culture substrate, a step of forming a sheet of the cell population into a sheet to form a sheet-shaped cell culture, and a step of detaching the formed sheet-shaped cell culture from the culture substrate. A method for treating liver dysfunction, including a step of applying the sheet-shaped cell culture to a site exhibiting the liver dysfunction.
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公开(公告)号：US20240277899A1
公开(公告)日：2024-08-22
申请号：US18648087
申请日：2024-04-26
Applicant: FUJIFILM Corporation , OSAKA UNIVERSITY
Inventor： Hayato MIYOSHI , Li LIU , Yoshiki SAWA , Junjun LI , Shigeru MIYAGAWA , Maki TAKEDA
CPC classification number: A61L27/38 , A61K35/34 , C12N5/0657 , C12N2502/1323
Abstract: An object of the present invention is to provide a production method of a myocardial cell layer, and a myocardial cell layer, in which myocardial cells can be matured without using a complicated control device and can be maintained for a long period of time, and to provide a kit for evaluating a pharmaceutical candidate substance, a transplantation material, and an evaluation method of a pharmaceutical candidate substance. According to the present invention, there is provided a production method of a myocardial cell layer including the following steps (1) to (3).
(1) seeding a myocardial cell induced from a pluripotent stem cell in a single layer on a seeding surface having a shape shown in the following (1-1) to form a myocardial cell layer,
(1-1) in a case where spread of the seeding surface in a horizontal direction is a plane figure, a shape thereof is a shape obtained by hollowing out at least a part of a first plane figure of an optional shape having uniform spread in a second plane figure of another optional shape having uniform spread without contacting an outer periphery of the first plane figure of the optional shape, where an average value of a length of the outer periphery of the first plane figure and a length of an outer periphery of the second plane figure satisfies the following formula;
in the formula, x represents the average value of the length of the outer periphery of the first plane figure and the length of the outer periphery of the second plane figure, where a unit thereof is cm, and v represents a conduction velocity of the myocardial cell induced from the pluripotent stem cell, where a unit thereof is cm·s−1,
(2) circulating and proceeding an electrical signal or a calcium signal in the myocardial cell layer, and
(3) culturing the cells for 8 days or more while maintaining a state of the (2).-
公开(公告)号：US20240182858A1
公开(公告)日：2024-06-06
申请号：US18282450
申请日：2022-03-16
Applicant: Astellas Pharma Inc. , Osaka University
Inventor： Kenichiro SHIMATANI , Hiromu SATO , Masao SASAI , Yoshiki SAWA , Atsuhiro SAITO , Shigeru MIYAGAWA
CPC classification number: C12N5/0661 , A61K35/44 , A61P7/00 , C07K14/503 , C12N5/10
Abstract: An object of the present invention is to provide a cell therapy that is expected to be useful as an angiogenic therapy for a peripheral vascular disease such as critical lower limb ischemia. Specifically, the object is to provide a pericyte having high angiogenic potential and a method for producing the same. A pericyte introduced a bFGF gene and a method for producing the same are provided.
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公开(公告)号：US20220372437A1
公开(公告)日：2022-11-24
申请号：US17765637
申请日：2020-09-09
Applicant: OSAKA UNIVERSITY
Inventor： Takaaki SAMURA , Shigeru MIYAGAWA , Yoshiki SAWA , Junya YOKOYAMA
Abstract: A method for producing a fibrin sheet containing at least one selected from the group consisting of cells and drugs in a fibrin gel, the method comprising: a step 1 of applying a fibrinogen solution containing at least one selected from the group consisting of cells and drugs and fibrinogen dropwise onto a surface of a substrate made of a gelatin hydrogel; a step 2 of adding thrombin to the fibrinogen solution on the surface of the substrate; a step 3 of placing a support film on and in contact with a top surface of the fibrinogen solution to which the thrombin has been added; a step 4 of forming a fibrin sheet containing the at least one selected from the group consisting of cells and drugs in a fibrin gel between the substrate and the support film by a reaction between the fibrinogen and the thrombin; and a step 5 of melting the substrate at a temperature not lower than a melting temperature of the gelatin hydrogel to separate, from the substrate, the fibrin sheet supported by the support film.
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公开(公告)号：US20170247658A1
公开(公告)日：2017-08-31
申请号：US15594011
申请日：2017-05-12
Applicant: TERUMO KABUSHIKI KAISHA , OSAKA UNIVERSITY
Inventor： Hiroko ISEOKA , Yoshiki SAWA , Shigeru MIYAGAWA , Satsuki FUKUSHIMA
CPC classification number: C12N5/0657 , A61K35/12 , A61K35/34 , A61L27/00 , C12N2506/45
Abstract: A sheet-shaped cell culture is disclosed that includes myocardial cells derived from pluripotent stem cells and has excellent functional properties. A sheet-shaped cell culture obtained by culturing a cell population that includes myocardial cells derived from pluripotent stem cells, wherein the proportion of the number of the myocardial cells derived from pluripotent stem cells to the total number of cells in the cell population is 50% to 70%; a method of producing the sheet-shaped cell culture; a composition including the sheet-shaped cell culture; and a method of treating a disease using the sheet-shaped cell culture or the composition.
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