公开(公告)号：US20160326584A1

公开(公告)日：2016-11-10

申请号：US15224460

申请日：2016-07-29

Applicant: Cellular Research, Inc.

Inventor： Stephen P.A. Fodor ,  Glenn K. Fu

IPC: C12Q1/68 ,  G06F19/22

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G06F19/20 ,  G06F19/22 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.

公开(公告)号：US20160265069A1

公开(公告)日：2016-09-15

申请号：US15167807

申请日：2016-05-27

Applicant: Cellular Research Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20160010151A1

公开(公告)日：2016-01-14

申请号：US14800526

申请日：2015-07-15

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20150005185A1

公开(公告)日：2015-01-01

申请号：US14281706

申请日：2014-05-19

Applicant: Cellular Research, Inc.

Inventor： Stephen P.A. Fodor ,  Glenn K. Fu

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G06F19/20 ,  G06F19/22 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.

Abstract translation: 公开了组合物，方法和试剂盒，用于通过附加不同标签组来随机标记相同分子的集合来进行高灵敏度单分子数字计数。 分子的每个拷贝从不同标签的非消耗性储库中随机选择。 检测可以通过多种方法，包括基于杂交或测序。 否则在信息内容中相同的分子可以被标记以产生在集合中是独特的或大致唯一的可单独检测的产物。 这种随机变换放宽了从定位和识别相同分子之一计数分子的问题到检测是否存在预编程标签的一系列二进制数字问题。 该方法可以用于例如估计样品中给定类型或类型的单独分子的数目。

公开(公告)号：US20180327836A1

公开(公告)日：2018-11-15

申请号：US16038832

申请日：2018-07-18

Applicant: Cellular Research, Inc.

Inventor： Stephen P.A. Fodor ,  Glenn K. Fu

IPC: C12Q1/6874 ,  G06F19/22 ,  C12Q1/6851 ,  G06F19/20 ,  C12N15/10 ,  C12Q1/6876 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q1/686 ,  C12Q1/6837

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G06F19/20 ,  G06F19/22 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.

公开(公告)号：US20180291470A1

公开(公告)日：2018-10-11

申请号：US16012584

申请日：2018-06-19

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/6888 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q1/6874

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20160289740A1

公开(公告)日：2016-10-06

申请号：US15084307

申请日：2016-03-29

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Stephen P.A. Fodor

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6869 ,  C12Q2563/185 ,  C12Q2525/173 ,  C12Q2525/185 ,  C12Q2525/191 ,  C12Q2525/204 ,  C12Q2531/119 ,  C12Q2535/122 ,  C12Q2563/107 ,  C12Q2565/514

Abstract: The present disclosure provide compositions, methods and kits for generating a set of combinatorial barcodes, and uses thereof for barcoding samples such as single cells or genomic DNA fragments. Some embodiments disclosed herein provide compositions comprising a set of component barcodes for producing a set of combinatorial barcodes. The set of component barcodes can comprise, for example, n×m unique component barcodes, wherein n and m are integers, each of the component barcodes comprises: one of n unique barcode subunit sequences; and one or two linker sequences or the complements thereof, wherein the component barcodes are configured to connect to each other through the one or two linker sequences or the complements thereof to produce a set of combinatorial barcodes.

Abstract translation: 本公开提供了用于产生一组组合条形码的组合物，方法和试剂盒及其用于条形码样品如单细胞或基因组DNA片段的用途。 本文公开的一些实施例提供了包括用于产生一组组合条形码的组合条形码组合的组合物。 组件条形码集合可以包括例如n×m个唯一组件条形码，其中n和m是整数，每个组成条形码包括：n个唯一条形码子单元序列之一; 和一个或两个接头序列或其互补物，其中组分条形码被配置为通过一个或两个接头序列或其互补体彼此连接以产生一组组合条形码。

公开(公告)号：US20160258012A2

公开(公告)日：2016-09-08

申请号：US14472363

申请日：2014-08-28

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

Abstract translation: 本公开提供了用于单个细胞的多重核酸分析的方法，组合物和试剂盒。 方法，组合物和系统可用于大规模并行单细胞测序。 方法，组成和系统可用于同时分析数千个细胞。 数千个细胞可以包含混合的细胞群体（例如，不同类型或亚型的细胞，不同大小的细胞）。

公开(公告)号：US20160253584A1

公开(公告)日：2016-09-01

申请号：US15055445

申请日：2016-02-26

Applicant: Cellular Research, Inc.

Inventor： Stephen P.A. Fodor ,  Christina Fan ,  Glenn Fu ,  Geoffrey Facer

IPC: G06K19/06

CPC classification number: G06K19/06103 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q2525/179 ,  C12Q2543/10 ,  C12Q2563/155

Abstract: The disclosure provides for methods, compositions, systems, devices, and kits for determining the number of distinct targets in distinct spatial locations within a sample. In some examples, the methods include: stochastically barcoding the plurality of targets in the sample using a plurality of stochastic barcodes, wherein each of the plurality of stochastic barcodes comprises a spatial label and a molecular label; estimating the number of each of the plurality of targets using the molecular label; and identifying the spatial location of each of the plurality of targets using the spatial label. The method can be multiplexed.

Abstract translation: 本公开提供了用于确定样品中不同空间位置中不同目标的数量的方法，组合物，系统，装置和试剂盒。 在一些示例中，所述方法包括：使用多个随机条形码随机条形码样本中的多个目标，其中所述多个随机条形码中的每一个包括空间标签和分子标签; 使用所述分子标签估计所述多个靶中的每一个的数量; 以及使用所述空间标签来识别所述多​​个目标中的每一个的空间位置。 该方法可以复用。

公开(公告)号：US20150119257A1

公开(公告)日：2015-04-30

申请号：US14326443

申请日：2014-07-08

Applicant: Cellular Research, Inc.

Inventor： Stephen P.A. Fodor ,  Glenn K. Fu

IPC: C12Q1/68

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12Q1/6809 ,  C12Q1/6837 ,  C12Q1/6851 ,  C12Q1/686 ,  C12Q1/6869 ,  C12Q1/6876 ,  G16B25/00 ,  G16B30/00 ,  C12Q2565/102 ,  C12Q2537/157 ,  C12Q2525/191

Abstract: Compositions, methods and kits are disclosed for high-sensitivity single molecule digital counting by the stochastic labeling of a collection of identical molecules by attachment of a diverse set of labels. Each copy of a molecule randomly chooses from a non-depleting reservoir of diverse labels. Detection may be by a variety of methods including hybridization based or sequencing. Molecules that would otherwise be identical in information content can be labeled to create a separately detectable product that is unique or approximately unique in a collection. This stochastic transformation relaxes the problem of counting molecules from one of locating and identifying identical molecules to a series of binary digital questions detecting whether preprogrammed labels are present. The methods may be used, for example, to estimate the number of separate molecules of a given type or types within a sample.