公开(公告)号：US20170337459A1

公开(公告)日：2017-11-23

申请号：US15667125

申请日：2017-08-02

Applicant: Cellular Research, Inc.

Inventor： Stephen P.A. Fodor ,  Christina Fan ,  Glenn Fu ,  Geoffrey Facer

IPC: G06K19/06 ,  C12Q1/68

CPC classification number: G06K19/06103 ,  C12Q1/6813 ,  C12Q1/6816 ,  C12Q2525/179 ,  C12Q2543/10 ,  C12Q2563/155

Abstract: The disclosure provides for methods, compositions, systems, devices, and kits for determining the number of distinct targets in distinct spatial locations within a sample. In some examples, the methods include: stochastically barcoding the plurality of targets in the sample using a plurality of stochastic barcodes, wherein each of the plurality of stochastic barcodes comprises a spatial label and a molecular label; estimating the number of each of the plurality of targets using the molecular label; and identifying the spatial location of each of the plurality of targets using the spatial label. The method can be multiplexed.

公开(公告)号：US09637799B2

公开(公告)日：2017-05-02

申请号：US14872377

申请日：2015-10-01

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20170073730A1

公开(公告)日：2017-03-16

申请号：US15260106

申请日：2016-09-08

Applicant: Cellular Research, Inc.

Inventor： Craig Betts ,  Glenn Fu

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12N15/1093 ,  C12N15/1096 ,  C12Q2525/191 ,  C12Q2537/159 ,  C12Q2523/305 ,  C12Q2525/155 ,  C12Q2525/186 ,  C12Q2531/113 ,  C12Q2537/143 ,  C12Q2563/131 ,  C12Q2563/143 ,  C12Q2563/149 ,  C12Q2535/122

Abstract: The disclosure provides for methods, compositions, and kits for normalizing nucleic acid libraries, for example sequencing libraries.

Abstract translation: 本公开提供了用于标准化核酸文库的方法，组合物和试剂盒，例如测序文库。

公开(公告)号：US20160257993A1

公开(公告)日：2016-09-08

申请号：US15055407

申请日：2016-02-26

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Julie Wilhelmy ,  Maria Simbirsky ,  Eleen Shum

IPC: C12Q1/68

CPC classification number: C12Q1/6806 ,  C12Q1/6844 ,  C12Q1/6869 ,  C12Q2525/155 ,  C12Q2525/191 ,  C12Q2535/122 ,  C12Q2563/179

Abstract: Embodiments provided herein relate to methods and compositions for labeling a nucleic acid in a sample with a stochastic barcode. Some embodiments relate to methods and compositions for characterizing a sample by identifying the TCR alpha chain or beta chain of a T cell.

Abstract translation: 本文提供的实施方案涉及用随机条形码标记样品中核酸的方法和组合物。 一些实施方案涉及通过鉴定T细胞的TCRα链或β链来表征样品的方法和组合物。

公开(公告)号：US20160145683A1

公开(公告)日：2016-05-26

申请号：US14872377

申请日：2015-10-01

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P.A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68

CPC classification number: C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881 ,  C12Q2600/158 ,  C12Q2600/16 ,  C12Q2563/159 ,  C12Q2563/185

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20160055632A1

公开(公告)日：2016-02-25

申请号：US14508911

申请日：2014-10-07

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Roger Rudoff ,  David Stern ,  George T. Wu ,  Stephen P.A. Fodor ,  Geoff Facer

IPC: G06T7/00 ,  G06T7/20

CPC classification number: G06T7/0012 ,  G01N21/274 ,  G01N21/6428 ,  G01N21/6452 ,  G01N21/6456 ,  G01N2021/6439 ,  G01N2201/129 ,  G06K9/6267 ,  G06T7/277 ,  G06T2207/10056 ,  G06T2207/10064 ,  G06T2207/30072 ,  G06T2207/30242

Abstract: Methods, systems and platforms for digital imaging of multiple regions of an array, and detection and counting of the labeled features thereon, are described.

Abstract translation: 描述了用于阵列的多个区域的数字成像的方法，系统和平台以及其上的标记特征的检测和计数。

公开(公告)号：US11845986B2

公开(公告)日：2023-12-19

申请号：US16374626

申请日：2019-04-03

Applicant: Cellular Research, Inc.

Inventor： Eleen Shum ,  Glenn Fu ,  Craig Betts

IPC: C12N15/10 ,  C12Q1/6874 ,  C12Q1/6806 ,  G16B25/00 ,  G16B25/10

CPC classification number: C12Q1/6874 ,  C12N15/1065 ,  C12N15/1093 ,  C12Q1/6806 ,  G16B25/00 ,  G16B25/10 ,  C12N15/1093 ,  C12Q2537/159 ,  C12N15/1093 ,  C12Q2537/159 ,  C12Q2563/179

Abstract: This disclosure provides methods and compositions for removing one or more high abundance species from a plurality of nucleic acid molecules. In some embodiments, the methods and compositions can be used for normalizing nucleic acid libraries. In some embodiments, molecular labels are used in conjunction with the methods and compositions disclosed herein to improve sequencing efficiency.

公开(公告)号：US11618929B2

公开(公告)日：2023-04-04

申请号：US16012584

申请日：2018-06-19

Applicant: Cellular Research, Inc.

Inventor： Christina Fan ,  Stephen P. A. Fodor ,  Glenn Fu ,  Geoffrey Richard Facer ,  Julie Wilhelmy

IPC: C12Q1/68 ,  C12Q1/6888 ,  C12Q1/6874 ,  C12Q1/6876 ,  C12Q1/6881

Abstract: The disclosure provides for methods, compositions, and kits for multiplex nucleic acid analysis of single cells. The methods, compositions and systems may be used for massively parallel single cell sequencing. The methods, compositions and systems may be used to analyze thousands of cells concurrently. The thousands of cells may comprise a mixed population of cells (e.g., cells of different types or subtypes, different sizes).

公开(公告)号：US20200332284A1

公开(公告)日：2020-10-22

申请号：US16836750

申请日：2020-03-31

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Eleen Shum

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6874 ,  C12Q1/6844

Abstract: The present disclosure relates to compositions, methods and kits for labeling an internal sequence of a target nucleic acid molecule with molecular barcodes. In some embodiments, the methods comprise intramolecular circulation of a labeled target nucleic acid molecule. Further provided methods for generating sequencing libraries comprising overlapping fragments covering the full length of a target nucleic acid molecule, sequencing the libraries using the methods disclosed herein, and methods of analyzing sequencing results therefrom.

公开(公告)号：US20200157600A1

公开(公告)日：2020-05-21

申请号：US16194819

申请日：2018-11-19

Applicant: Cellular Research, Inc.

Inventor： Glenn Fu ,  Craig Betts ,  Christina Fan ,  Gretchen Yinbon Lam

IPC: C12Q1/6806 ,  C12Q1/6837 ,  C12Q1/6855 ,  C12N9/00 ,  C12N9/50 ,  C12N15/10

Abstract: The disclosure provides for methods, compositions, systems, devices, and kits for whole transcriptome amplification using stochastic barcodes.