-
公开(公告)号：US11795426B2
公开(公告)日：2023-10-24
申请号：US17402047
申请日：2021-08-13
Applicant: CORNING INCORPORATED
Inventor： Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral
CPC classification number: C12M25/02 , C12M23/02 , C12M23/20 , C12M23/44 , C12M25/14 , C12M29/10 , C12M29/26
Abstract: A fixed-bed bioreactor system is provided that includes a vessel with a media inlet, a media outlet, and an interior cavity disposed between and in fluid communication with the media inlet and media outlet. The vessel further includes a cell culture substrate disposed in the interior cavity between the media inlet and the media outlet in a packed-bed configuration, the cell culture substrate including a plurality of porous disks in a stacked arrangement. The interior cavity includes a cell culture section and a spacer section, the cell culture substrate defining the cell culture section and the spacer section being disposed between the cell culture section and the media outlet, and each of the plurality of porous disks has a surface configured to culture cells thereon.
-
公开(公告)号：US20220372421A1
公开(公告)日：2022-11-24
申请号：US17766045
申请日：2020-09-29
Applicant: CORNING INCORPORATED
Inventor： Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral
Abstract: Kits for making a spheroid-stabilizing hydrogel in a calcium-free or calcium-chelated cell culture media include (a) a gelation agent including a polygalacturonic acid (PGA) compound or an alginic acid compound, wherein the PGA compound includes at least one of: (i) pectic acid or salts thereof, or (ii) partially esterified pectic acid having a degree of esterification from about 1 to about 40 mol % or salts thereof; (b) a crosslinking agent, wherein the crosslinking agent includes a salt of a divalent ion; and (c) a proton donor, wherein the proton donor includes lactones, esters, or other compounds that hydrolyze in aqueous solutions to form acids over a period of from 10 minutes to 1 hour. Resultant spheroid-stabilizing hydrogels and methods of preparing the same.
-
13.发明申请公开(公告)号：US20200248124A1
公开(公告)日：2020-08-06
申请号：US16781883
申请日：2020-02-04
Applicant: CORNING INCORPORATED
Inventor： Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral , Lori E. Romeo
Abstract: A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.
-
公开(公告)号：US20200248122A1
公开(公告)日：2020-08-06
申请号：US16781764
申请日：2020-02-04
Applicant: CORNING INCORPORATED
Inventor： Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral , Lori E. Romeo
Abstract: A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.
-
公开(公告)号：US20200148989A1
公开(公告)日：2020-05-14
申请号：US16628309
申请日：2018-07-13
Applicant: CORNING INCORPORATED
Inventor： Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral , Jeffrey Glenn Lynn
Abstract: A cell culture vessel (100) has walls and a substrate having a plurality of microcavities (120), where each microcavity of the plurality of microcavities includes a concave well and an opening to allow the microcavity to be filled with liquid. A flange (170) surrounds the substrate having an array of microcavities. A channel (175, 176) surrounds the flange, providing a moat around the microcavity substrate. The flange is angled. Methods of culturing cells in the cell culture vessel are also provided.
-
Patent Agency Ranking
公开(公告)号：US20170342363A1
公开(公告)日：2017-11-30
申请号：US15492690
申请日：2017-04-20
Applicant: Corning Incorporated
Inventor： Ye Fang , Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral , Gregory Roger Martin , Kaitlyn Mary Matias , Michael Kurt Schaefer , Allison Jean Tanner
Abstract: The present disclosure relates to apparatuses, systems and methods for culturing cells. In particular, devices and methods are provided for generation and culture of 3d cell aggregates.
-
公开(公告)号：US20150275167A1
公开(公告)日：2015-10-01
申请号：US14663533
申请日：2015-03-20
Applicant: Corning Incorporated
Inventor： Ronald Allen Faris , Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral , Gregory Roger Martin , Jin Liu
CPC classification number: A61K9/2866
Abstract: An ex vivo cell culture sustained release composition, including: a mixture comprising: a sustenant; and a non-biodegradable binder; and a non-biodegradable and water insoluble encapsulant coat that encapsulates the mixture, as defined herein. Also disclosed is a method for sustainably providing a sustenant to a cell culture in aqueous media, including contacting a cell culture with the sustained release composition.
Abstract translation: 一种离体细胞培养持续释放组合物，包括：包含：维生素的混合物; 和不可生物降解的粘合剂; 以及包封如本文所定义的混合物的不可生物降解的水不溶性包封剂涂层。 还公开了一种用于将水分介质中的细胞培养物可持续地提供给细胞培养物的方法，包括使细胞培养物与持续释放组合物接触。
-
公开(公告)号：US20150268222A1
公开(公告)日：2015-09-24
申请号：US14437275
申请日：2013-11-15
Applicant: CORNING INCORPORATED
Inventor： Oberon Denaci Deichmann , Ye Fang , Ann MeeJin Ferrie , Haibei Hu , Qi Wu
CPC classification number: G01N33/5014 , G01N15/1429 , G01N15/1434 , G01N15/1456 , G01N15/1463 , G01N21/3151 , G01N21/7743 , G01N33/502 , G01N33/5061 , G01N2015/0065 , G01N2015/144 , G01N2015/1454
Abstract: A label-free resonant waveguide grating biosensor imager system for measuring beat patterns and dynamic mass redistribution (DMR) signals of cultured cardiomyocytes in the absence and presence of a drug molecule. The disclosure also provides a method using the imager system for analyzing the beat patterns and the DMR signals of the cardiomyocytes to assess drug-induced cardiotoxicity.
Abstract translation: 一种无标记的谐振波导光栅生物传感器成像系统，用于在不存在和存在药物分子的情况下测量培养的心肌细胞的搏动模式和动态质量再分配（DMR）信号。 本公开还提供了一种使用成像仪系统分析心肌细胞的搏动模式和DMR信号以评估药物诱导的心脏毒性的方法。
-
公开(公告)号：US12037572B2
公开(公告)日：2024-07-16
申请号：US18206501
申请日：2023-06-06
Applicant: CORNING INCORPORATED
Inventor： Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral , Lori Eileen Romeo
CPC classification number: C12M25/14 , C12M23/02 , C12M23/20 , C12M23/44 , C12M25/04 , C12M29/10 , C12M29/26 , C12M41/26 , C12M25/02 , C12N5/0068 , C12N5/0602 , C12N2513/00 , C12N2533/30 , C12N2533/40 , C12N2533/90 , C12N2535/00
Abstract: A cell culture matrix is provided that has a substrate with a first side, a second side opposite the first side, a thickness separating the first side and the second side, and a plurality of openings formed in the substrate and passing through the thickness of the substrate. The plurality of openings allow flow of at least one of cell culture media, cells, or cell products through the thickness of the substrate, and provides a uniform, efficient, and scalable matrix for cell seeding, proliferation, and culturing. The substrate can be formed from a woven polymer mesh material that provides a high surface area to volume ratio for cells and good fluid flow through the matrix. Bioreactor systems incorporating the cell culture matrix and related methods are also provided.
-
公开(公告)号：US20240026287A1
公开(公告)日：2024-01-25
申请号：US18226559
申请日：2023-07-26
Applicant: CORNING INCORPORATED
Inventor： Ye Fang , Ann MeeJin Ferrie , Vasiliy Nikolaevich Goral , David Henry , Martial Hervy , Corinne Walerack , Yue Zhou
IPC: C12N5/00 , C08J3/24 , C09D167/00
CPC classification number: C12N5/0075 , C12N5/0068 , C08J3/24 , C09D167/00 , C12N2533/40 , C12N2533/50 , C12N2537/10 , C12N2533/32 , C12N2533/74
Abstract: A cell culture article includes a substrate having a polymer coating that is conducive to colony passaging of cells cultured on the coating. Example polymer coatings are formed from polygalacturonic acid (PGA), alginate, or combinations thereof. Cells cultured on the polymer coating can be separated from the substrate as a colony or layer of cells by exposing the polymer coating to (i) a chelating agent, (ii) a proteinase-free enzyme, or (iii) a chelating agent and a proteinase-free enzyme.
-
-
-
-
-
-
-
-
-
Search Results
55
records
(took 0.021 seconds)
