公开(公告)号：US20100291130A1

公开(公告)日：2010-11-18

申请号：US12637696

申请日：2009-12-14

申请人： Jens Bukh ,  Robert H. Purcell ,  Suzanne U. Emerson ,  Akito Sakai ,  Patrizia Farci

发明人： Jens Bukh ,  Robert H. Purcell ,  Suzanne U. Emerson ,  Akito Sakai ,  Patrizia Farci

IPC分类号： A61K39/29 ,  C07K7/06 ,  C07K7/08 ,  C07K14/08 ,  C12P21/02 ,  C12Q1/70 ,  C12Q1/37 ,  A61P31/14

CPC分类号： G01N33/5767 ,  A61K39/12 ,  A61K39/29 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/24221 ,  C12N2770/24222 ,  C12N2770/24234 ,  G01N2500/04 ,  G01N2500/10

摘要： Embodiments described herein include nucleic acid sequences, which encode hepatitis C virus of strain HC-TN, genotype 1a, proteins and polypeptides and fragments thereof. Use of these compositions, and diagnostics for HCV and in the development of screening assays for the identification of antiviral agents for HCV are also contemplated.

摘要翻译： 本文描述的实施方案包括编码菌株HC-TN的丙型肝炎病毒，基因型1a，蛋白质和多肽及其片段的核酸序列。 还考虑使用这些组合物和HCV的诊断以及开发用于鉴定HCV的抗病毒剂的筛选测定法。

公开(公告)号：US20090252755A1

公开(公告)日：2009-10-08

申请号：US12061504

申请日：2008-04-02

申请人： Jens Bukh ,  Robert H. Purcell ,  Suzanne U. Emerson ,  Akito Sakai

发明人： Jens Bukh ,  Robert H. Purcell ,  Suzanne U. Emerson ,  Akito Sakai

IPC分类号： A61K39/00 ,  C12N15/11 ,  C12N15/00 ,  C12N5/06 ,  C07K14/00 ,  A61P37/00 ,  C12N15/87 ,  C12Q1/70 ,  C12Q1/34 ,  C12Q1/37

CPC分类号： G01N33/5767 ,  A61K39/12 ,  A61K39/29 ,  C07K14/005 ,  C12N7/00 ,  C12N2770/24221 ,  C12N2770/24222 ,  C12N2770/24234 ,  G01N2500/04 ,  G01N2500/10

摘要： Embodiments described herein include nucleic acid sequences, which encode hepatitis C virus of strain HC-TN, genotype 1a, proteins and polypeptides and fragments thereof. Use of these compositions, and diagnostics for HCV and in the development of screening assays for the identification of antiviral agents for HCV are also contemplated.

摘要翻译： 本文描述的实施方案包括编码菌株HC-TN的丙型肝炎病毒，基因型1a，蛋白质和多肽及其片段的核酸序列。 还考虑使用这些组合物和HCV的诊断以及开发用于鉴定HCV的抗病毒剂的筛选测定法。

公开(公告)号：US06458562B1

公开(公告)日：2002-10-01

申请号：US09402776

申请日：2000-02-22

申请人： Suzanne U. Emerson ,  Robert H. Purcell ,  Sergei A. Tsarev ,  Robin A. Robinson

发明人： Suzanne U. Emerson ,  Robert H. Purcell ,  Sergei A. Tsarev ,  Robin A. Robinson

IPC分类号： C12N1551

CPC分类号： C07K14/005 ,  C12N2770/28122 ,  Y10S977/804

摘要： The invention relates to the expression of open reading frame 2 (ORF-2) proteins of a strain of hepatitis E virus from Pakistan (SAR-55) in a eukaryotic expression system. The expressed proteins can serve as an antigen in diagnostic immunoassays and/or as an immunogen or vaccine to protect against infection by hepatitis E.

摘要翻译： 本发明涉及在真核表达系统中来自巴基斯坦（SAR-55）的乙型肝炎病毒株的开放阅读框2（ORF-2）蛋白的表达。 表达的蛋白质可以用作诊断免疫测定中的抗原和/或作为免疫原或疫苗以防止乙型肝炎的感染。

公开(公告)号：US06287759B1

公开(公告)日：2001-09-11

申请号：US08471971

申请日：1995-06-06

申请人： Sergei A. Tsarev ,  Suzanne U. Emerson ,  Robert H. Purcell

发明人： Sergei A. Tsarev ,  Suzanne U. Emerson ,  Robert H. Purcell

IPC分类号： C12Q170

CPC分类号： C07K14/005 ,  A61K38/00 ,  A61K39/00 ,  C07H21/00 ,  C12N7/00 ,  C12N15/1131 ,  C12N2310/3125 ,  C12N2310/314 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/341 ,  C12N2310/345 ,  C12N2310/346 ,  C12N2710/14143 ,  C12N2770/28021 ,  C12N2770/28022 ,  C12N2770/28122 ,  C12N2770/28123 ,  Y10S977/802 ,  C12N2310/3521

摘要： A strain of hepatitis E virus from Pakistan (SAR-55) implicated in an epidemic of enterically transmitted non-A, non-B hepatitis, now called hepatitis E, is disclosed. The invention relates to the expression of the whole structural region of SAR-55, designated open reading frame 2 (ORF-2), in a eukaryotic expression system. The expressed protein is capable of forming HEV virus-like particles which can serve as an antigen in diagnostic immunoassays and as an immunogen or vaccine to protect against infection by hepatitis E.

公开(公告)号：US06207416B1

公开(公告)日：2001-03-27

申请号：US08809523

申请日：1997-05-28

申请人： Sergei A. Tsarev ,  Suzanne U. Emerson ,  Robert H. Purcell

发明人： Sergei A. Tsarev ,  Suzanne U. Emerson ,  Robert H. Purcell

IPC分类号： C12N1551

CPC分类号： C07H21/00 ,  A61K38/00 ,  A61K39/00 ,  C07K14/005 ,  C12N7/00 ,  C12N15/1131 ,  C12N2310/3125 ,  C12N2310/314 ,  C12N2310/315 ,  C12N2310/321 ,  C12N2310/341 ,  C12N2310/345 ,  C12N2310/346 ,  C12N2710/14143 ,  C12N2770/28021 ,  C12N2770/28022 ,  C12N2770/28122 ,  C12N2770/28123 ,  C12N2310/3521

摘要： A strain of hepatitis E virus from Pakistan (SAR-55) implicated in an epidemic of enterically transmitted non-A, non-B hepatitis, now called hepatitis E, is disclosed. The invention relates to the expression of the whole structural region of SAR-55, designated open reading frame 2 (ORF-2), in a eukaryotic expression system. The expressed protein is capable of forming HEV virus-like particles which can serve as an antigen in diagnostic immunoassays and as an immunogen or vaccine to protect against infection by hepatitis E.

摘要翻译： 披露了一种来自巴基斯坦的丙型肝炎病毒（SAR-55），涉及到目前被称为戊型肝炎的非传染性非乙型肝炎的流行病。 本发明涉及真核表达系统中SAR-55整体结构域的表达，其命名为开放阅读框架2（ORF-2）。 表达的蛋白质能够形成HEV病毒样颗粒，其可以在诊断免疫测定中作为抗原，并作为免疫原或疫苗以防止乙型肝炎感染。

公开(公告)号：US6113912A

公开(公告)日：2000-09-05

申请号：US475886

申请日：1995-06-07

申请人： Ann W. Funkhouser ,  Suzanne U. Emerson ,  Robert H. Purcell ,  Eric D'Hondt

发明人： Ann W. Funkhouser ,  Suzanne U. Emerson ,  Robert H. Purcell ,  Eric D'Hondt

IPC分类号： C12N15/09 ,  A61K35/76 ,  A61K39/00 ,  A61K39/29 ,  A61P31/12 ,  C07K14/10 ,  C12N7/00 ,  C12N7/01 ,  C12N7/08 ,  C12N15/51 ,  C12P21/02 ,  C12R1/91 ,  H61K39/29

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/29 ,  C12N7/00 ,  A61K2039/5254 ,  A61K2039/542 ,  A61K2039/545 ,  C12N2770/32421 ,  C12N2770/32422 ,  C12N2770/32432 ,  C12N2770/32434

摘要： A live hepatitis A virus adapted to growth in MRC-5 cells, which HAV is preferably characterized by suitable attenuation for effective vaccine administration to humans and animals without inactivation, methods for adapting HAV to growth in MRC-5, vaccine compositions and method of vaccinating humans against HAV infection.

公开(公告)号：US5849562A

公开(公告)日：1998-12-15

申请号：US468926

申请日：1995-06-06

申请人： Robert H. Purcell ,  Suzanne U. Emerson

发明人： Robert H. Purcell ,  Suzanne U. Emerson

IPC分类号： A61K39/00 ,  A61K39/29 ,  C07K14/10 ,  C12Q1/70 ,  C12N5/16

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/29 ,  C12Q1/706 ,  A61K39/00 ,  C12N2770/32422 ,  C12N2770/32434

摘要： Methods for producing HAV cDNA, products thereof, and uses thereof, are described. HAV cDNA is produced, for example, by reverse transcribing HAV RNA and subsequently inserting the HAV cDNA into bacterial plasmids by genetic-engineering techniques. Transformed bacteria are then cloned and cultured to produce replicated chimeric plasmids containing the HAV cDNA. Such HAV cDNA is useful in assaying for the presence of HAV and in the production of HAV antigen and in the production of antibodies against HAV.

摘要翻译： 描述了生产HAV cDNA的方法及其用途及其用途。 例如，通过逆转录HAV RNA并随后通过遗传工程技术将HAV cDNA插入到细菌质粒中产生HAV cDNA。 然后克隆和培养转化的细菌以产生含有HAV cDNA的复制嵌合质粒。 这样的HAV cDNA可用于测定HAV的存在和HAV抗原的产生和抗HAV抗体的产生。

公开(公告)号：US08574853B2

公开(公告)日：2013-11-05

申请号：US13310463

申请日：2011-12-02

申请人： Zhaochun Chen ,  Robert H. Purcell ,  Suzanne U. Emerson ,  Stephen H. Leppla ,  Mahtab Moayeri

发明人： Zhaochun Chen ,  Robert H. Purcell ,  Suzanne U. Emerson ,  Stephen H. Leppla ,  Mahtab Moayeri

IPC分类号： G01N33/53

CPC分类号： C07K16/1278 ,  A61K51/1009 ,  A61K2039/505 ,  C07K16/40 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/51 ,  C07K2317/515 ,  C07K2317/55 ,  C07K2317/565 ,  C07K2317/76 ,  C07K2317/92 ,  G01N33/56911 ,  G01N33/573 ,  G01N2333/32 ,  G01N2333/954 ,  G01N2469/10

摘要： The present invention relates to monoclonal antibodies that bind or neutralize anthrax lethal factor (LF), edema factor (EF), and/or protective antigen (PA). The invention provides such antibodies, fragments of such antibodies retaining anthrax toxin-binding ability, fully human or humanized antibodies retaining anthrax toxin-binding ability, and pharmaceutical compositions including such antibodies. The invention further provides for isolated nucleic acids encoding the antibodies of the invention and host cells transformed therewith. Additionally, the invention provides for prophylactic, therapeutic, and diagnostic methods employing the antibodies and nucleic acids of the invention.

公开(公告)号：US20110097270A1

公开(公告)日：2011-04-28

申请号：US11795255

申请日：2006-01-13

申请人： Darren J. Schofield ,  Suzanne U. Emerson ,  Robert H. Purcell

发明人： Darren J. Schofield ,  Suzanne U. Emerson ,  Robert H. Purcell

IPC分类号： A61K49/00 ,  C07K16/08 ,  C07H21/00 ,  C12N15/63 ,  C12N5/10 ,  A61K39/395 ,  C12Q1/70 ,  A61P31/20 ,  A61P37/04

CPC分类号： C07K16/082 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/55

摘要： The present invention relates to the isolation and characterization of a novel neutralizing chimpanzee monoclonal antibody to hepatitis B virus. The invention provides such antibodies, fragments of such antibodies retaining hepatitis B virus-binding ability, fully human or humanized antibodies retaining hepatitis B virus-binding ability, and pharmaceutical compositions including such antibodies. The invention further provides for isolated nucleic acids encoding the antibodies of the invention and host cells transformed therewith. Additionally, the invention provides for prophylactic, therapeutic, and diagnostic methods employing the antibodies and nucleic acids of the invention.

摘要翻译： 本发明涉及一种用于乙型肝炎病毒的新型中和黑猩猩单克隆抗体的分离和表征。 本发明提供了这样的抗体，保留乙型肝炎病毒结合能力的这种抗体的片段，保留乙型肝炎病毒结合能力的完全人或人源化抗体，以及包含这种抗体的药物组合物。 本发明还提供了编码本发明的抗体的分离的核酸和用其转化的宿主细胞。 另外，本发明提供了使用本发明的抗体和核酸的预防，治疗和诊断方法。

公开(公告)号：US20100074892A1

公开(公告)日：2010-03-25

申请号：US11793735

申请日：2005-12-21

申请人： Zhaochun Chen ,  Stephen Leppla ,  Mahtab Moayeri ,  Suzanne U. Emerson ,  Robert H. Purcell

发明人： Zhaochun Chen ,  Stephen Leppla ,  Mahtab Moayeri ,  Suzanne U. Emerson ,  Robert H. Purcell

IPC分类号： A61K39/395 ,  C07K16/00 ,  C07H21/04 ,  C12N5/10 ,  G01N33/53 ,  A61P31/04

CPC分类号： C07K16/1278 ,  A61K2039/505 ,  C07K2317/21 ,  C07K2317/24 ,  C07K2317/52 ,  C07K2317/622 ,  C07K2317/76 ,  C07K2319/00

摘要： The present invention relates to monoclonal antibodies that bind or neutralize anthrax protective antigen (PA) toxin. The invention provides such antibodies, fragments of such antibodies retaining anthrax PA toxin-binding ability, fully human or humanized antibodies retaining anthrax PA toxin-binding ability, and pharmaceutical compositions including such antibodies. The invention further provides for isolated nucleic acids encoding the antibodies of the invention and host cells transformed therewith. Additionally, the invention provides for prophylactic, therapeutic, and diagnostic methods employing the antibodies and nucleic acids of the invention.

摘要翻译： 本发明涉及结合或中和炭疽保护性抗原（PA）毒素的单克隆抗体。 本发明提供了这样的抗体，这种抗体的片段，其保留了炭疽PA毒素结合能力，完全人或人源化抗体保留了炭疽杆菌PA毒素结合能力，以及包含这些抗体的药物组合物。 本发明还提供了编码本发明的抗体的分离的核酸和用其转化的宿主细胞。 另外，本发明提供了使用本发明的抗体和核酸的预防，治疗和诊断方法。