Method of production of poly-.beta.-hydroxyalkanoate copolymers
    20.
    发明授权
    Method of production of poly-.beta.-hydroxyalkanoate copolymers 失效
    聚-β-羟基链烷酸酯共聚物的制备方法

    公开(公告)号:US5891686A

    公开(公告)日:1999-04-06

    申请号:US881562

    申请日:1997-06-24

    摘要: The present invention provides methods for the production of poly-.beta.-hydroxyalkanoate copolymer comprising the steps of (a) introducing into a prokaryotic host cell a vector construct which directs the expression of a sequence which encodes a poly-.beta.-hydroxybutyrate biosynthetic pathway, (b) introducing into the host cell a vector construct which directs the expression of one or more proteins which regulate acetate and propionate metabolism, (c) culturing the host cell in medium containing propionate or a derivative thereof, and (d) isolating poly-.beta.-hydroxyalkanoate copolymer from the cultured host cell.

    摘要翻译: 本发明提供了生产聚-β-羟基链烷酸酯共聚物的方法,包括以下步骤:(a)向原核宿主细胞中引入指导编码聚-β-羟基丁酸生物合成途径的序列表达的载体构建体( b)将宿主细胞引入到宿主细胞中,该载体构建体引导一种或多种调节乙酸盐和丙酸盐代谢的蛋白质的表达,(c)在含有丙酸盐或其衍生物的培养基中培养宿主细胞,和(d)分离多聚β - 羟基链烷酸酯共聚物。