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105 条记录 (耗时 0.026 秒)

    Thermostable Taq polymerase fragment
    13.
    发明授权
    Thermostable Taq polymerase fragment 有权
    热稳定性Taq聚合酶片段

    公开(公告)号：US07972830B2

    公开(公告)日：2011-07-05

    申请号：US10917157

    申请日：2004-08-12

    申请人： Thomas Meier ,  Waltraud Ankenbauer ,  Annette Deufel ,  Dieter Heindl ,  Gisela Betzl ,  Rainer Schmuck ,  Bernd Schneidinger ,  Jessica Strey

    发明人： Thomas Meier ,  Waltraud Ankenbauer ,  Annette Deufel ,  Dieter Heindl ,  Gisela Betzl ,  Rainer Schmuck ,  Bernd Schneidinger ,  Jessica Strey

    IPC分类号： C12N9/12

    CPC分类号： C12N9/1252

    摘要： It was found that a fragment of native Thermus aquaticus DNA polymerase (TaqWT) lacking 288 N-terminal amino acids (TaqΔ288) possesses an increased thermostability over TaqWT, TaqΔ279, and TaqΔ289. The present invention therefore provides TaqΔ288, recombinant expression vectors encoding the same or derivatives thereof, as well as purification protocols for TaqΔ288. The invention also encompasses kits containing TaqΔ288 as well as the use of TaqΔ288 and kits containing TaqΔ288. In addition, the invention encompasses methods for the sequencing a nucleic acid template and methods for amplifying a target nucleic acid.

    摘要翻译： 发现缺乏288个N-末端氨基酸（Taq＆Dgr; 288）的天然的水生栖热菌水生DNA聚合酶（TaqWT）的片段具有比TaqWT，Taq＆Dgr; 279和Taq＆Dgr; 289增加的热稳定性。 因此，本发明提供Taq＆Dgr; 288，编码其相同或衍生物的重组表达载体，以及Taq＆Dgr; 288的纯化方案。 本发明还包括含有Taq＆Dgr; 288的试剂盒，以及使用Taq＆Dgr; 288和含有Taq＆Dgr; 288的试剂盒。 此外，本发明包括用于测序核酸模板的方法和用于扩增靶核酸的方法。

    METHOD FOR DETECTION OF AN RNA MOLECULE, A KIT AND USE RELATED THEREFOR
    14.
    发明申请
    METHOD FOR DETECTION OF AN RNA MOLECULE, A KIT AND USE RELATED THEREFOR 审中-公开
    用于检测RNA分子的方法，与其相关的试剂盒及其用途

    公开(公告)号：US20110151444A1

    公开(公告)日：2011-06-23

    申请号：US12968322

    申请日：2010-12-15

    申请人： Andreas Albers ,  Waltraud Ankenbauer ,  Frank Bergmann ,  Stefanie Froehner ,  Dieter Heindl

    发明人： Andreas Albers ,  Waltraud Ankenbauer ,  Frank Bergmann ,  Stefanie Froehner ,  Dieter Heindl

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/686 ,  C12Q2525/207 ,  C12Q2525/186 ,  C12Q2525/155

    摘要： Described is a method for the detection of a RNA molecule, the method involving the steps of providing a sample containing the RNA molecule; hybridizing to the RNA molecule a first polynucleotide; extending the first polynucleotide to generate a first strand cDNA; hybridizing a second polynucleotide to the first strand cDNA; extending the first strand cDNA to generate an extension reaction product; amplifying the extension reaction product by means of polymerase chain reaction; and detecting the amplification product by means of real-time fluorescence readout. Also described is a kit containing a first and a second polynucleotide as defined in the present invention, a set of dNTPs, a reverse transcriptase enzyme, and a detection moiety.

    摘要翻译： 描述了用于检测RNA分子的方法，该方法包括提供含有RNA分子的样品的步骤; 与RNA分子杂交第一多核苷酸; 扩展第一多核苷酸以产生第一链cDNA; 将第二多核苷酸与第一链cDNA杂交; 延伸第一链cDNA以产生延伸反应产物; 通过聚合酶链反应扩增延伸反应产物; 并通过实时荧光读数检测扩增产物。 还描述了包含本发明中定义的第一和第二多核苷酸的试剂盒，一组dNTP，逆转录酶和检测部分。

    TYPE OF UNIVERSAL PROBE FOR THE DETECTION OF GENOMIC VARIANTS
    18.
    发明申请
    TYPE OF UNIVERSAL PROBE FOR THE DETECTION OF GENOMIC VARIANTS 审中-公开
    通用探头类型，用于检测基因组变体

    公开(公告)号：US20120225428A1

    公开(公告)日：2012-09-06

    申请号：US13411902

    申请日：2012-03-05

    申请人： Reinhard Beck ,  Frank Bergmann ,  Rita Haerteis ,  Dieter Heindl ,  Ralf Mauritz ,  Heiko Walch

    发明人： Reinhard Beck ,  Frank Bergmann ,  Rita Haerteis ,  Dieter Heindl ,  Ralf Mauritz ,  Heiko Walch

    IPC分类号： C12Q1/68 ,  G01N21/64

    CPC分类号： C12Q1/6832 ,  C12Q1/6827 ,  C12Q2525/101 ,  C12Q2525/204

    摘要： The present disclosure relates to a composition comprising a first set of probes and a second set of probes, composed of one or more DNA nucleotide(s) and five or more LNA (locked nucleic acid) nucleotides, wherein the base at a discriminating position differs for a first probe of the first set and a first probe of the second set. The present disclosure relates to the composition comprising a plurality of probes in each of the first and second set of probes, wherein the probes in each set differ in one, two, or three LNA random position(s). Further, the present disclosure relates to a method of detecting genomic variants by means of the aforementioned probes.

    摘要翻译： 本公开涉及包含由一个或多个DNA核苷酸和五个或更多个LNA（锁定的核酸）核苷酸组成的第一组探针和第二组探针的组合物，其中鉴别位置处的碱基不同 用于第一组的第一探针和第二组的第一探针。 本公开涉及在第一和第二组探针中的每一个中包括多个探针的组合物，其中每组中的探针在一个，两个或三个LNA随机位置上不同。 此外，本公开涉及通过上述探针检测基因组变体的方法。

    DIRECTED SYNTHESIS OF OLIGOPHOSPHORAMIDATE STEREOISOMERS
    19.
    发明申请
    DIRECTED SYNTHESIS OF OLIGOPHOSPHORAMIDATE STEREOISOMERS 失效
    指导性合成寡糖磷酸酯STEREOISOMERS

    公开(公告)号：US20110212861A1

    公开(公告)日：2011-09-01

    申请号：US13099630

    申请日：2011-05-03

    申请人： Dieter Heindl ,  Dirk Kessler ,  Angelika Roesler ,  Christoph Seidel ,  Wilma Thuer

    发明人： Dieter Heindl ,  Dirk Kessler ,  Angelika Roesler ,  Christoph Seidel ,  Wilma Thuer

    IPC分类号： C40B40/04 ,  C07F9/24 ,  G01N33/68

    CPC分类号： G01N33/5308 ,  C07F9/6552 ,  C07F9/65586 ,  Y02P20/55

    摘要： The trivalent phosphorous atom of a compound is reacted with a reagent in such a manner that a stable phosphate mimetic or a specifier is formed. Phosphoramidites with a phosphorous atom containing at least one hydroxyl residue which is provided with a protective group are reacted for this purpose with a free hydroxyl group: In the first synthesis cycle the hydroxyl group is linked to a solid support via a cleavable or non-cleavable linker. In further synthesis cycles the hydroxyl group is created by cleavage of the protective group from the growing oligomer. This results in formation of a phosphorous acid triester which is reacted with azides. By selecting suitable monomers for the synthesis which have a defined stereoconformation compounds of Formula 1 are produced in a stereocontrolled manner.

    摘要翻译： 使化合物的三价磷原子与试剂反应，形成稳定的磷酸酯模拟物或说明物。 具有至少一个含有保护基团的羟基残基的磷原子的亚磷酰胺与该游离羟基反应：在第一个合成循环中，羟基通过可裂解或不可切割的方式与固体支持物连接 接头。 在进一步的合成循环中，通过从生长的低聚物中切割保护基而产生羟基。 这导致形成与叠氮化物反应的亚磷酸三酯。 通过选择具有定义的立体定构的合成合成单体，以立体控制的方式制备式1的化合物。

    Labeling reagent
    20.
    发明授权
    Labeling reagent 有权
    标记试剂

    公开(公告)号：US07759470B2

    公开(公告)日：2010-07-20

    申请号：US11707399

    申请日：2007-02-16

    申请人： Dieter Heindl ,  Frank Bergmann ,  Hans-Peter Josel ,  Dirk Kessler ,  Gunter Lampert ,  Christian Weilke

    发明人： Dieter Heindl ,  Frank Bergmann ,  Hans-Peter Josel ,  Dirk Kessler ,  Gunter Lampert ,  Christian Weilke

    IPC分类号： C07G3/00 ,  C07H21/02 ,  C07H21/04 ,  C07H19/04 ,  C12Q1/68

    CPC分类号： C07H19/23 ,  C07H19/04 ,  C07H21/00 ,  C07H21/04 ,  Y02P20/55

    摘要： The current invention restates substituted indole nucleosides as both terminal as well as internal building blocks of labeled oligonucleotide probes for the detection, analysis and quantitation of nucleic acids. The substituent comprises a linker and a detectable group or a linker and a reactive group for post synthesis coupling. These modified nucleosides grant access to a wide application area. These new substituted indole nucleosides can be used as labeling reagents for the facile preparation of e.g. optimized hybridization probes, Taqman-probes, or molecular beacon probes.

    摘要翻译： 本发明将取代的吲哚核苷作为两个末端以及用于核酸检测，分析和定量的标记的寡核苷酸探针的内部构建块重述。 取代基包括接头和可检测的基团或接头以及用于后合成偶联的反应性基团。 这些修饰的核苷允许进入广泛的应用领域。 这些新的取代的吲哚核苷可以用作标记试剂，用于容易地制备例如 优化的杂交探针，Taqman探针或分子信标探针。