公开(公告)号：US20110207229A1

公开(公告)日：2011-08-25

申请号：US13124407

申请日：2009-10-06

申请人： Toon Hendrik Evers ,  Wendy Uyen Dittmer

发明人： Toon Hendrik Evers ,  Wendy Uyen Dittmer

IPC分类号： G01N33/00

CPC分类号： G01N33/54326 ,  B82Y25/00 ,  G01N27/745 ,  G01N35/0098 ,  G01R33/093 ,  G01R33/1269

摘要： The invention relates to a method and a device (100) for determining the amount of a target component (2) in a sample, wherein magnetic particles (2) can bind to a contact surface (4) with kinetics that depend on the sample-amount of the target component. The method comprises at least two washing steps during which magnetic particles (2) are magnetically moved away from the contact surface (4) and corresponding measurements of the remaining amount of magnetic particles (2) at the contact surface (4). The amount of target component in the sample is estimated from at least one of such measurement results. The measurement allows to determine also high concentrations of target component for which the sensor surface (4) is saturated in a steady-state.

摘要翻译： 本发明涉及一种用于确定样品中目标组分（2）的量的方法和装置（100），其中磁性颗粒（2）可以结合取决于样品的动力学的接触表面（4） 目标成分的数量。 该方法包括至少两个洗涤步骤，在此过程中，磁性颗粒（2）被磁性地远离接触表面（4）移动，并且在接触表面（4）处磁性颗粒（2）剩余量的相应测量值。 从这些测量结果中的至少一个估计样品中的目标组分的量。 该测量允许确定传感器表面（4）在稳定状态下饱和的目标部件的高浓度。

公开(公告)号：US20140057366A1

公开(公告)日：2014-02-27

申请号：US13988833

申请日：2011-11-28

申请人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus dionisius Van Zon

发明人： Wendy Uyen Dittmer ,  Mikhail Mikhaylovich Ovsyanko ,  Toon Hendrik Evers ,  Jeroen Hans Nieuwenhuis ,  Joannes Baptist Adrianus dionisius Van Zon

IPC分类号： G01N33/543

CPC分类号： G01N33/54373 ,  G01N21/274 ,  G01N21/552 ,  G01N27/72 ,  G01N27/745 ,  G01N27/825 ,  G01N33/54326 ,  G01N35/0098

摘要： The invention relates to a sensor device (100) and a method for the detection of magnetic particles (1) in a sample chamber (2) with a contact surface (11). The sensor device (100) comprises a sensor unit (120, 130) for detecting magnetic particles (1) in a target region (TR) and/or in at least one reference region on the contact surface. Moreover, it comprises a magnetic field generator (140) for generating a magnetic field that shall guide magnetic particles to the contact surface. With the help of these components, an “auxiliary parameter” is determined that is related to the magnetic particles (1) and/or their movement but that is independent of binding processes taking place in the target region between magnetic particles and the contact surface. The auxiliary parameter may for example be related to the degree of mismatch between the positions reached by the magnetic particles (1) under the influence of a magnetic field and the target region (TR). The evaluation results can be used to validate and/or correct the measurements obtained in the target region (TR).

摘要翻译： 本发明涉及一种传感器装置（100）和用于在具有接触表面（11））的样品室（2）中检测磁性颗粒（1）的方法。 传感器装置（100）包括用于检测目标区域（TR）中和/或接触表面上的至少一个参考区域中的磁性颗粒（1）的传感器单元（120,130）。 此外，它包括用于产生磁场的磁场发生器（140），该磁场将磁性颗粒引导到接触表面。 在这些组件的帮助下，确定与磁性颗粒（1）和/或它们的运动相关的“辅助参数”，但是与磁性颗粒和接触表面之间的目标区域中发生的结合过程无关。 辅助参数可以例如与在磁场和目标区域（TR）的影响下由磁性颗粒（1）达到的位置之间的失配程度相关。 评估结果可用于验证和/或校正在目标区域（TR）中获得的测量结果。

公开(公告)号：US20130109106A1

公开(公告)日：2013-05-02

申请号：US13808522

申请日：2011-07-04

申请人： Derk Jan Wilfred Klunder ,  Jeroen Hans Nieuwehuis ,  Menno Willem Jose Prins ,  Toon Hendrik Evers

发明人： Derk Jan Wilfred Klunder ,  Jeroen Hans Nieuwehuis ,  Menno Willem Jose Prins ,  Toon Hendrik Evers

IPC分类号： G01N1/10

CPC分类号： G01N1/10 ,  B01L3/0275 ,  B01L3/527 ,  B01L7/52 ,  B01L2200/04 ,  B01L2200/0684 ,  B01L2200/16 ,  B01L2300/027 ,  B01L2300/044 ,  B01L2300/0627 ,  B01L2300/0654 ,  B01L2300/087 ,  B01L2400/043 ,  G01N35/00029 ,  G01N35/0098 ,  G01N35/1002 ,  G01N2035/00158 ,  G01N2035/0436 ,  Y10T436/2575

摘要： The invention relates to a method and a system (100) for selectively processing a sample (130) according to one of a plurality of different assays, for example for detecting a certain target component in the sample. The system comprises a plurality of “specific reagent reservoirs” (120) that contain different sets of reagents, wherein each set is required for one of the assays. Moreover, the system (100) comprises a “universal reagent reservoir” in which reagents for several assays are provided, preferably reagents for all assays. The universal reagent reservoir may preferably be a cartridge (110) in which the processing of a sample (130) can take place and which comprises a plurality of binding sites that are selective for different target components. Depending on the assay to be performed with a sample (130) at hand, the appropriate specific reagent reservoir (121b) is chosen and processed in the universal cartridge (110). This can particularly be done by a manipulator (140) in a high-throughput automated laboratory system.

摘要翻译： 本发明涉及用于根据多种不同测定中的一种选择性处理样品（130）的方法和系统（100），例如用于检测样品中某一目标成分。 该系统包括多个含有不同试剂组的“特异性试剂储存器”（120），其中每种试剂均需要每组。 此外，系统（100）包括“通用试剂储库”，其中提供用于几种测定的试剂，优选用于所有测定的试剂。 通用试剂储存器可以优选地是其中样品（130）的处理可以发生并且包括对不同目标组分具有选择性的多个结合位点的盒（110）。 根据将要用手的样品（130）进行的测定，在通用盒（110）中选择并处理适当的特定试剂储存器（121b）。 这可以通过高通量自动化实验室系统中的机械手（140）来进行。

公开(公告)号：US20120252033A1

公开(公告)日：2012-10-04

申请号：US13515853

申请日：2010-12-10

申请人： Joannes Baptist Adrianus Dionisius Van Zon ,  Toon Hendrik Evers ,  Maatje Koets

发明人： Joannes Baptist Adrianus Dionisius Van Zon ,  Toon Hendrik Evers ,  Maatje Koets

IPC分类号： G01N33/53 ,  G01N33/566 ,  G01N30/00

CPC分类号： G01N21/552 ,  G01N33/54373

摘要： The invention relates to a substance determining apparatus and method for determining a substance within a fluid. Particles attach to the substance and bind to a binding surface (30), wherein a particle release curve being indicative of a release of bound particles from the binding surface (30) is determined, and a part of the particle release curve caused by particles bound to the binding surface via a predefined kind of binding is determined based on the temporal behaviour of the particle release curve. The substance within the fluid is determined based on the part of the particle release curve. The substance can therefore be determined based on particles which are bound to the binding surface via a certain kind of binding, i.e. other kinds of binding substantially do not affect the determination of the substance, thereby improving the accuracy of determining the substance.

摘要翻译： 本发明涉及一种用于确定流体内的物质的物质确定装置和方法。 颗粒附着于物质并结合至结合表面（30），其中确定指示结合颗粒从结合表面（30）释放的颗粒释放曲线，并且由颗粒结合引起的部分颗粒释放曲线 基于粒子释放曲线的时间行为来确定经由预定类型的结合的结合表面。 基于颗粒释放曲线的部分确定流体内的物质。 因此，物质可以基于通过某种结合结合到结合表面的颗粒来确定，即其它种类的结合基本上不影响物质的测定，从而提高了确定物质的准确性。

公开(公告)号：US20120178186A1

公开(公告)日：2012-07-12

申请号：US13497813

申请日：2010-09-10

申请人： Jeroen Hans Nieuwennhuis ,  Toon Hendrik Evers

发明人： Jeroen Hans Nieuwennhuis ,  Toon Hendrik Evers

IPC分类号： G01N27/72

CPC分类号： G01N33/54326 ,  G01N27/745

摘要： The present invention relates to a cartridge (1) for the detection of an analyte (2) in a binding assay with magnetic labels (3), the cartridge comprising at least one capture binding agent (4) against a binding site on the analyte and comprising at least two magnetically labelled tracer binding agents (51 and 52) against further different binding sites on the analyte, characterised in that the cartridge comprises at least two regions (61 and 62) wherein a first region (61) comprises the at least one capture binding agent (4) and a first magnetically labelled tracer binding agent (51) and wherein a second region (62) comprises at least one capture binding agent (4) and a second magnetically labelled tracer binding agent (52). Herein the first region (61) does not comprise a magnetically labelled tracer binding agent other than the first magnetically labelled tracer bind agent (51) and wherein the second region (62) does not comprise a magnetically labelled tracer binding agent other than the second magnetically labelled tracer binding agent (52).

摘要翻译： 本发明涉及一种用于在具有磁性标签（3）的结合测定中检测分析物（2）的盒（1），所述盒包含针对分析物上的结合位点的至少一种捕获结合剂（4） 包括至少两个磁性标记的示踪剂结合剂（51和52），其抵抗所述分析物上的另外不同的结合位点，其特征在于，所述盒包括至少两个区域（61和62），其中第一区域（61）包括所述至少一个 捕获结合剂（4）和第一磁性标记的示踪剂结合剂（51），并且其中第二区域（62）包含至少一种捕获结合剂（4）和第二磁性标记示踪剂结合剂（52）。 本文中，第一区域（61）不包含除了第一磁性标记的示踪剂结合剂（51）之外的磁性标记的示踪剂结合剂，并且其中第二区域（62）不包括除了第二磁性标记的磁性标记的示踪剂结合剂 标记示踪剂结合剂（52）。

公开(公告)号：US20110244596A1

公开(公告)日：2011-10-06

申请号：US13124426

申请日：2009-10-06

申请人： Toon Hendrik Evers ,  Mikhail Mikhaylovich Ovsyanko

发明人： Toon Hendrik Evers ,  Mikhail Mikhaylovich Ovsyanko

IPC分类号： G01N33/543 ,  G01N27/72

摘要： A method for controlling the movement of magnetic or magnetizable objects (10) in a biosensor cartridge. The method comprises the step of providing a biosensor cartridge with a laterally extending sensor surface (A) and at least a magnetic field generating means (20, 30, 30′) for generating a magnetic field with a field gradient substantially perpendicular to the sensor surface (A). The magnetic field generating means (20, 30, 30′) are alternatingly actuated such that the generated magnetic field directs alternatingly the magnetic or magnetizable objects (10) substantially perpendicular to the sensor surface (A) away and toward the sensor surface, wherein pulse lengths of the alternating actuation are adjusted such that a lateral movement of magnetizable objects along the laterally extending sensor surface is substantially avoided.

摘要翻译： 一种用于控制生物传感器盒中的磁性或可磁化物体（10）的运动的方法。 该方法包括以下步骤：向生物传感器盒提供横向延伸的传感器表面（A）和至少一个用于产生具有基本上垂直于传感器表面的场梯度的磁场的磁场产生装置（20,30,30'） （一个）。 磁场产生装置（20,30,30'）被交替地致动，使得所产生的磁场交替地引导基本上垂直于传感器表面（A）的磁性或可磁化物体（10）远离传感器表面，其中脉冲 调整交替致动的长度，使得可沿着横向延伸的传感器表面的可磁化物体的横向移动被基本上避免。

公开(公告)号：US10794903B2

公开(公告)日：2020-10-06

申请号：US13124426

申请日：2009-10-06

申请人： Toon Hendrik Evers ,  Mikhail Mikhaylovich Ovsyanko

发明人： Toon Hendrik Evers ,  Mikhail Mikhaylovich Ovsyanko

IPC分类号： G01N33/543 ,  B03C5/02 ,  B03C1/28 ,  G01N27/74 ,  B01L3/00 ,  G01R33/12

摘要： A method for controlling the movement of magnetic or magnetizable objects (10) in a biosensor cartridge. The method comprises the step of providing a biosensor cartridge with a laterally extending sensor surface (A) and at least a magnetic field generating means (20, 30, 30′) for generating a magnetic field with a field gradient substantially perpendicular to the sensor surface (A). The magnetic field generating means (20, 30, 30′) are alternatingly actuated such that the generated magnetic field directs alternatingly the magnetic or magnetizable objects (10) substantially perpendicular to the sensor surface (A) away and toward the sensor surface, wherein pulse lengths of the alternating actuation are adjusted such that a lateral movement of magnetizable objects along the laterally extending sensor surface is substantially avoided.

公开(公告)号：US09720003B2

公开(公告)日：2017-08-01

申请号：US13141439

申请日：2009-12-15

申请人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Peggy De Kievit ,  Ricky Kamps ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens ,  David Walterus Cornelis Dekkers

发明人： Wendy Uyen Dittmer ,  Toon Hendrik Evers ,  Peggy De Kievit ,  Ricky Kamps ,  Joost Lambert Max Vissers ,  Michael Franciscus Wilhelmus Cornelis Martens ,  David Walterus Cornelis Dekkers

IPC分类号： G01N33/68 ,  G01N33/543

CPC分类号： G01N33/6887 ,  G01N33/54373 ,  G01N2800/324

摘要： The present invention relates to a method for measuring Troponin I in a sample comprising the steps of providing a sample, contacting the sample with a monoclonal anti-Troponin I antibody coupled to a magnetic label, contacting the sample with a polyclonal anti-Troponin I antibody coupled to a sensor surface and detecting the magnetic label on the sensor surface. The invention further relates to a device and a cartridge for measuring Troponin I in a sample.

公开(公告)号：US09612239B2

公开(公告)日：2017-04-04

申请号：US14001566

申请日：2012-02-27

申请人： Joannes Baptist Adrianus Dionisius Van Zon ,  Toon Hendrik Evers ,  Ron Martinus Laurentius Van Lieshout ,  Wilhelmina Maria Hardeman ,  Derk Jan Wilfred Klunder

发明人： Joannes Baptist Adrianus Dionisius Van Zon ,  Toon Hendrik Evers ,  Ron Martinus Laurentius Van Lieshout ,  Wilhelmina Maria Hardeman ,  Derk Jan Wilfred Klunder

IPC分类号： G01N21/17 ,  G01N33/543 ,  G01N21/552 ,  G01N21/64 ,  G01N21/77 ,  G01N27/74

CPC分类号： G01N33/54326 ,  G01N21/552 ,  G01N21/648 ,  G01N21/7703 ,  G01N27/745

摘要： The invention relates to a substance determining apparatus (19) for determining a substance within a fluid. A location event determination unit (34) determines location events indicating that a particle, which may have attached the substance, is located in a sensing region and a property of the location events from the sensing signal generated by sensing the sensing region. A location events selection unit (70) selects a part of the location events having a property in a predefined property range, and a substance determination unit (40) determines the substance within the fluid (3) based on the selected part of the location events. This allows tuning the determination of the substance within the fluid to a desired property range, which can relate to a desired kind of bound particles, thereby reducing the influence of unwanted effects on the determination of the substance and, thus, improving the determination accuracy.

公开(公告)号：US09612236B2

公开(公告)日：2017-04-04

申请号：US13704258

申请日：2011-06-14

申请人： Gwenola Sabatte ,  Menno Willem Jose Prins ,  Toon Hendrik Evers ,  Wilhelmina Maria Hardeman ,  Joukje Garrelina Orsel

发明人： Gwenola Sabatte ,  Menno Willem Jose Prins ,  Toon Hendrik Evers ,  Wilhelmina Maria Hardeman ,  Joukje Garrelina Orsel

IPC分类号： G01N33/53 ,  G01N33/543

CPC分类号： G01N33/5306 ,  G01N33/54306 ,  G01N33/54333

摘要： The present invention is related to a method for detection of a biological target in an affinity assay, the method comprising the steps of providing a biological sample volume containing the biological target, adding a first capturing moiety to the biological sample volume comprising the biological target, wherein the first capturing moiety is adhered to a particle, concentration of the captured biological target into an elution volume that is smaller than the biological sample volume in step a), cleavage of the first capturing moiety or the biological target from the particle and direct or indirect detection and/or quantification of the biological target in a sandwich or competitive affinity assay format, wherein the biological target is associated with at least one capturing moiety, preferably at least two capturing moieties.