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    Novel processes for non-linearly amplifying nucleic acids using a primer with three segments
    14.
    发明申请
    Novel processes for non-linearly amplifying nucleic acids using a primer with three segments 审中-公开
    使用具有三个片段的引物非线性扩增核酸的新方法

    公开(公告)号：US20050260573A1

    公开(公告)日：2005-11-24

    申请号：US10303152

    申请日：2002-11-22

    申请人： Elazar Rabbani ,  Jannis Stavrianopoulos ,  James Donegan ,  Jack Coleman ,  Marleen Walner

    发明人： Elazar Rabbani ,  Jannis Stavrianopoulos ,  James Donegan ,  Jack Coleman ,  Marleen Walner

    IPC分类号： C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q1/6869

    CPC分类号： C12Q1/6869 ,  C12P19/34 ,  C12Q1/6844 ,  C12Q1/6853 ,  C12Q2531/119 ,  C12Q2525/301 ,  C12Q2525/161

    摘要： This invention provides novel processes for amplifying nucleic acid sequences of interest, including linear and non-linear amplification. In linear amplification, a single initial primer or nucleic acid construct is utilized to carry out the amplification process. In non-linear amplification, a first initial primer or nucleic acid construct is employed with a subsequent initial primer or nucleic acid construct. In other non-linear amplification processes provided by this invention, a first initial primer or nucleic acid construct is deployed with a second initial primer or nucleic acid construct to amplify the specific nucleic acid sequence of interest and its complement that are provided. A singular primer or a singular nucleic acid construct capable of non-linear amplification can also be used to carry out non-linear amplification in accordance with this invention. Post-termination labeling process for nucleic acid sequencing is also disclosed in this invention that is based upon the detection of tagged molecules that are covalently bound to chemically reactive groups provided for chain terminators. A process for producing nucleic acid sequences having decreased thermodynamic stability to complementary sequences is also provided and achieved by this invention. Unique nucleic acid polymers are also disclosed and provided in addition to other novel compositions, kits and the like.

    摘要翻译： 本发明提供用于扩增感兴趣的核酸序列的新方法，包括线性和非线性扩增。 在线性扩增中，使用单个初始引物或核酸构建体进行扩增过程。 在非线性扩增中，使用第一初始引物或核酸构建体与随后的初始引物或核酸构建体。 在本发明提供的其它非线性扩增方法中，用第二初始引物或核酸构建物展开第一个初始引物或核酸构建体，以扩增所提供的特异性核酸序列及其互补序列。 也可以使用能够进行非线性扩增的单一引物或单个核酸构建体来进行本发明的非线性扩增。 用于核酸测序的终止后标记方法也在本发明中公开，其基于共价结合到为链终止剂提供的化学反应性基团的标记分子的检测。 通过本发明提供并实现了对互补序列产生具有降低的热力学稳定性的核酸序列的方法。 除了其它新型组合物，试剂盒等之外，还公开并提供了独特的核酸聚合物。