-
1.发明申请Novel processes for non-linearly amplifying nucleic acids using a primer with three segments 审中-公开使用具有三个片段的引物非线性扩增核酸的新方法公开(公告)号：US20050260573A1
公开(公告)日：2005-11-24
申请号：US10303152
申请日：2002-11-22
IPC分类号： C12N15/09 , C12P19/34 , C12Q1/68 , C12Q1/6844 , C12Q1/6853 , C12Q1/6869
CPC分类号： C12Q1/6869 , C12P19/34 , C12Q1/6844 , C12Q1/6853 , C12Q2531/119 , C12Q2525/301 , C12Q2525/161
摘要： This invention provides novel processes for amplifying nucleic acid sequences of interest, including linear and non-linear amplification. In linear amplification, a single initial primer or nucleic acid construct is utilized to carry out the amplification process. In non-linear amplification, a first initial primer or nucleic acid construct is employed with a subsequent initial primer or nucleic acid construct. In other non-linear amplification processes provided by this invention, a first initial primer or nucleic acid construct is deployed with a second initial primer or nucleic acid construct to amplify the specific nucleic acid sequence of interest and its complement that are provided. A singular primer or a singular nucleic acid construct capable of non-linear amplification can also be used to carry out non-linear amplification in accordance with this invention. Post-termination labeling process for nucleic acid sequencing is also disclosed in this invention that is based upon the detection of tagged molecules that are covalently bound to chemically reactive groups provided for chain terminators. A process for producing nucleic acid sequences having decreased thermodynamic stability to complementary sequences is also provided and achieved by this invention. Unique nucleic acid polymers are also disclosed and provided in addition to other novel compositions, kits and the like.
摘要翻译： 本发明提供用于扩增感兴趣的核酸序列的新方法，包括线性和非线性扩增。 在线性扩增中，使用单个初始引物或核酸构建体进行扩增过程。 在非线性扩增中，使用第一初始引物或核酸构建体与随后的初始引物或核酸构建体。 在本发明提供的其它非线性扩增方法中，用第二初始引物或核酸构建物展开第一个初始引物或核酸构建体，以扩增所提供的特异性核酸序列及其互补序列。 也可以使用能够进行非线性扩增的单一引物或单个核酸构建体来进行本发明的非线性扩增。 用于核酸测序的终止后标记方法也在本发明中公开，其基于共价结合到为链终止剂提供的化学反应性基团的标记分子的检测。 通过本发明提供并实现了对互补序列产生具有降低的热力学稳定性的核酸序列的方法。 除了其它新型组合物，试剂盒等之外，还公开并提供了独特的核酸聚合物。
-
2.发明申请Novel compositions and processes for analyte detection, quantification and amplification 有权用于分析物检测，定量和扩增的新型组合物和方法公开(公告)号：US20060099601A1
公开(公告)日：2006-05-11
申请号：US10900453
申请日：2004-07-27
CPC分类号： C40B40/06 , B01J19/0046 , B01J2219/00599 , B01J2219/0061 , B01J2219/00612 , B01J2219/00648 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C07H21/00 , C12N15/1006 , C12N15/1058 , C12Q1/68 , C12Q1/6809 , C12Q1/6825 , C12Q1/6837 , C40B40/00 , C40B40/10 , G01N33/68 , C12Q2565/501 , C12Q2563/179 , C12Q2545/114 , C12Q2525/155 , C12Q2565/514 , C12Q2565/537
摘要： This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
摘要翻译： 本发明提供了用于分析物检测，定量和扩增的新型组合物和方法。 分析物的核酸阵列和文库可有效地并入到这样的组合物和方法中。 使用通用检测元件，信号实体等来检测并且如果需要或期望量化分析物。 靶分析物的扩增也由本发明的组合物和方法提供。
-
公开(公告)号：US20060040271A1
公开(公告)日：2006-02-23
申请号：US10902567
申请日：2004-07-29
IPC分类号： C40B40/08
CPC分类号： C40B40/06 , B01J19/0046 , B01J2219/00599 , B01J2219/0061 , B01J2219/00612 , B01J2219/00648 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C07H21/00 , C12N15/1006 , C12N15/1058 , C12Q1/68 , C12Q1/6809 , C12Q1/6825 , C12Q1/6837 , C40B40/00 , C40B40/10 , G01N33/68 , C12Q2565/501 , C12Q2563/179 , C12Q2545/114 , C12Q2525/155 , C12Q2565/514 , C12Q2565/537
摘要： This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
-
4.发明申请公开(公告)号：US20060024735A1
公开(公告)日：2006-02-02
申请号：US11236151
申请日：2005-09-27
CPC分类号： C12Q1/6818 , C12Q1/6834 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , G01N21/64 , G01N21/6486 , C12Q2565/101 , C12Q2533/101 , C12Q2561/113
摘要： This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided.
-
公开(公告)号：US20060014156A1
公开(公告)日：2006-01-19
申请号：US10902682
申请日：2004-07-29
CPC分类号： C40B40/06 , B01J19/0046 , B01J2219/00599 , B01J2219/0061 , B01J2219/00612 , B01J2219/00648 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C07H21/00 , C12N15/1006 , C12N15/1058 , C12Q1/68 , C12Q1/6809 , C12Q1/6825 , C12Q1/6837 , C40B40/00 , C40B40/10 , G01N33/68 , C12Q2565/501 , C12Q2563/179 , C12Q2545/114 , C12Q2525/155 , C12Q2565/514 , C12Q2565/537
摘要： This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
摘要翻译： 本发明提供了用于分析物检测，定量和扩增的新型组合物和方法。 分析物的核酸阵列和文库可有效地并入到这样的组合物和方法中。 使用通用检测元件，信号实体等来检测并且如果需要或期望量化分析物。 靶分析物的扩增也由本发明的组合物和方法提供。
-
公开(公告)号：US20050202456A1
公开(公告)日：2005-09-15
申请号：US10902640
申请日：2004-07-29
IPC分类号： G01N33/53 , C07B61/00 , C07H21/00 , C07K17/02 , C12M1/00 , C12N15/09 , C12Q1/00 , C40B40/02 , C40B50/06 , G01N33/58 , G01N37/00 , C12Q1/68 , C07H21/02 , C07K14/00
CPC分类号： C40B40/06 , B01J19/0046 , B01J2219/00599 , B01J2219/0061 , B01J2219/00612 , B01J2219/00648 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C07H21/00 , C12N15/1006 , C12N15/1058 , C12Q1/68 , C12Q1/6809 , C12Q1/6825 , C12Q1/6837 , C40B40/00 , C40B40/10 , G01N33/68 , C12Q2565/501 , C12Q2563/179 , C12Q2545/114 , C12Q2525/155 , C12Q2565/514 , C12Q2565/537
摘要： This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
摘要翻译： 本发明提供了用于分析物检测，定量和扩增的新型组合物和方法。 分析物的核酸阵列和文库可有效地并入到这样的组合物和方法中。 使用通用检测元件，信号实体等来检测并且如果需要或期望量化分析物。 靶分析物的扩增也由本发明的组合物和方法提供。
-
7.发明申请公开(公告)号：US20060029968A1
公开(公告)日：2006-02-09
申请号：US11235516
申请日：2005-09-26
CPC分类号： C12Q1/6818 , C12Q1/6834 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , G01N21/64 , G01N21/6486 , C12Q2565/101 , C12Q2533/101 , C12Q2561/113
摘要： This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided.
摘要翻译： 本发明提供用于实时核酸检测过程的组合物。 这种实时核酸检测方法是用连接于核酸引物，核苷酸，核酸探针或核酸结合剂的能量转移元件进行的。 实时核酸检测允许定性或定量检测或测定样品中感兴趣的单链或双链核酸。 本发明提供了其它方法，包括从被分析物或分析物库中除去一部分均聚物序列，例如聚A序列或尾部的方法。 还描述和提供了用于进行这种去除方法的组合物。
-
8.发明申请Composition of matter comprising library of analytes hybridized to nucleic acid array for generating complex formation and signaling domains 审中-公开包含与核酸阵列杂交的分析物文库以产生复合物形成和信号结构域的物质组成公开(公告)号：US20050233343A1
公开(公告)日：2005-10-20
申请号：US10900452
申请日：2004-07-27
IPC分类号： G01N33/53 , C07B61/00 , C07H21/00 , C07K17/02 , C12M1/00 , C12N15/09 , C12Q1/00 , C40B40/02 , C40B50/06 , G01N33/58 , G01N37/00 , C12Q1/68 , C12M1/34
CPC分类号： C40B40/06 , B01J19/0046 , B01J2219/00599 , B01J2219/0061 , B01J2219/00612 , B01J2219/00648 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C07H21/00 , C12N15/1006 , C12N15/1058 , C12Q1/68 , C12Q1/6809 , C12Q1/6825 , C12Q1/6837 , C40B40/00 , C40B40/10 , G01N33/68 , C12Q2565/501 , C12Q2563/179 , C12Q2545/114 , C12Q2525/155 , C12Q2565/514 , C12Q2565/537
摘要： This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
摘要翻译： 本发明提供了用于分析物检测，定量和扩增的新型组合物和方法。 分析物的核酸阵列和文库可有效地并入到这样的组合物和方法中。 使用通用检测元件，信号实体等来检测并且如果需要或期望量化分析物。 靶分析物的扩增也由本发明的组合物和方法提供。
-
9.发明申请公开(公告)号：US20050214784A1
公开(公告)日：2005-09-29
申请号：US10902641
申请日：2004-07-29
IPC分类号： G01N33/53 , C07B61/00 , C07H21/00 , C07K17/02 , C12M1/00 , C12N15/09 , C12Q1/00 , C40B40/02 , C40B50/06 , G01N33/58 , G01N37/00 , C12Q1/68 , C07H21/04
CPC分类号： C40B40/06 , B01J19/0046 , B01J2219/00599 , B01J2219/0061 , B01J2219/00612 , B01J2219/00648 , B01J2219/00722 , B01J2219/00725 , C07B2200/11 , C07H21/00 , C12N15/1006 , C12N15/1058 , C12Q1/68 , C12Q1/6809 , C12Q1/6825 , C12Q1/6837 , C40B40/00 , C40B40/10 , G01N33/68 , C12Q2565/501 , C12Q2563/179 , C12Q2545/114 , C12Q2525/155 , C12Q2565/514 , C12Q2565/537
摘要： This invention provides novel compositions and processes for analyte detection, quantification and amplification. Nucleic acid arrays and libraries of analytes are usefully incorporated into such compositions and processes. Universal detection elements, signaling entities and the like are employed to detect and if necessary or desirable, to quantify analytes. Amplification of target analytes are also provided by the compositions and processes of this invention.
摘要翻译： 本发明提供了用于分析物检测，定量和扩增的新型组合物和方法。 分析物的核酸阵列和文库可有效地并入这种组合物和方法中。 使用通用检测元件，信号实体等来检测并且如果需要或期望量化分析物。 靶分析物的扩增也由本发明的组合物和方法提供。
-
公开(公告)号：US20050137388A1
公开(公告)日：2005-06-23
申请号：US10096076
申请日：2002-03-12
CPC分类号： C12Q1/6818 , C12Q1/6834 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , G01N21/64 , G01N21/6486 , C12Q2565/101 , C12Q2533/101 , C12Q2561/113
摘要： This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided.
摘要翻译： 本发明提供用于实时核酸检测过程的组合物。 这种实时核酸检测方法是用连接于核酸引物，核苷酸，核酸探针或核酸结合剂的能量转移元件进行的。 实时核酸检测允许定性或定量检测或测定样品中感兴趣的单链或双链核酸。 本发明提供了其它方法，包括从被分析物或分析物库中除去一部分均聚物序列，例如聚A序列或尾部的方法。 还描述和提供了用于进行这种去除方法的组合物。
-
-
-
-
-
-
-
-
-
搜索结果
37 条记录 (耗时 0.034 秒)
