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公开(公告)号：US20180327512A1
公开(公告)日：2018-11-15
申请号：US15525688
申请日：2015-11-11
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav Rodrigo , Tomas Bjorkman , Mats Ander
IPC: C07K16/46 , B01J20/289 , B01J20/32 , B01D15/38
CPC classification number: C07K16/46 , B01D15/3809 , B01J20/289 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K14/31 , C07K16/00 , C07K16/065
Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO 26 or SEQ ID NO 27, wherein at least the alanine residue at the position corresponding to position 42 in SEQ ID NO:4-7 has been mutated to arginine and/or wherein at least the aspartic acid residue at the position corresponding to position 37 in SEQ ID NO:4-7 has been mutated to glutamic acid.
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公开(公告)号：US20170334954A1
公开(公告)日：2017-11-23
申请号：US15526941
申请日：2015-11-16
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav Rodrigo , Tomas Bjorkman , Mats Ander
CPC classification number: C07K16/46 , B01D15/3809 , B01J20/289 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K14/31 , C07K16/00 , C07K16/065
Abstract: An Fc-binding polypeptide of improved alkali stability, comprising a mutant of an Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 1, SEQ ID NO: 2, SEQ ID NO:3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:22, SEQ ID NO 51 or SEQ ID NO 52 wherein at least the asparagine or serine residue at the position corresponding to position 11 in SEQ ID NO:4-7 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
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公开(公告)号：US20200317725A1
公开(公告)日：2020-10-08
申请号：US16911511
申请日：2020-06-25
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Annika Forss , Mats Ander , Tomas Bjorkman , Hans Blom , Jesper Hansson , Gustav Rodrigo
IPC: C07K1/16 , C07K16/00 , B01J20/286 , B01J20/285 , B01D15/18 , B01D15/34 , C07K1/22 , C07K14/31
Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising at least 15 mg/ml multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, wherein the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml; b) contacting a liquid sample comprising an immunoglobulin with the separation matrix; c) washing the separation matrix with a washing liquid; d) eluting the immunoglobulin from the separation matrix with an elution liquid; and e) cleaning the separation matrix with a cleaning liquid comprising at least 0.5 M NaOH.
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公开(公告)号：US20200283475A1
公开(公告)日：2020-09-10
申请号：US16884475
申请日：2020-05-27
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Annika Kristina Forss , Gustav Jose Rodrigo , Tomas Bjorkman , Jesper Ulf Hansson , Mats Ander
Abstract: The invention relates to a method of isolating an immunoglobulin, comprising the steps of: a) providing a separation matrix comprising multimers of immunoglobulin-binding alkali-stabilized Protein A domains covalently coupled to a porous support, b) contacting a liquid sample comprising an immunoglobulin with the separation matrix, c) washing said separation matrix with a washing liquid, d) eluting the immunoglobulin from the separation matrix with an elution liquid, and e) cleaning the separation matrix with a cleaning liquid, wherein the alkali-stabilized Protein A domains comprise mutants of a parental Fc-binding domain of Staphylococcus Protein A (SpA), as defined by SEQ ID NO: 51 or SEQ ID NO: 52, wherein the amino acid residues at positions 13 and 44 of SEQ ID NO: 51 or 52 are asparagines and wherein at least the asparagine residue at position 3 of SEQ ID NO: 51 or 52 has been mutated to an amino acid selected from the group consisting of glutamic acid, lysine, tyrosine, threonine, phenylalanine, leucine, isoleucine, tryptophan, methionine, valine, alanine, histidine and arginine.
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公开(公告)号：US20200071353A1
公开(公告)日：2020-03-05
申请号：US16682855
申请日：2019-11-13
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav José Rodrigo , Tomas Bjorkman , Mats Ander , Jesper Ulf Hansson
IPC: C07K1/22 , C07K16/00 , C07K14/31 , B01J20/28 , B01J20/24 , B01D15/38 , C07K17/10 , B01J20/286 , B01J20/32 , C07K16/06
Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
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Patent Agency Ranking
公开(公告)号：US10208092B2
公开(公告)日：2019-02-19
申请号：US15526895
申请日：2015-12-11
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav Rodrigo , Mats Ander , Tomas Bjorkman
IPC: C07K14/195 , C07K1/22 , C07K16/00
Abstract: The invention discloses kappa light chain-binding polypeptide comprising a mutated binding domain of Peptostreptococcus protein L, wherein at least one asparagine residue of a parental domain defined by, or having at least 95% or 98% sequence homology with, SEQ ID NO: 2-6 or 2 has been mutated to another amino acid residue which is not asparagine, proline or cysteine.
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公开(公告)号：US09896486B2
公开(公告)日：2018-02-20
申请号：US14903837
申请日：2014-07-08
Applicant: GE Healthcare BioProcess R&D AB
Inventor： Gustav Rodrigo , Mats Ander , Tomas Bjorkman , Goran Bauren
IPC: C07K14/31 , C07K1/22 , B01D15/38 , B01J20/286 , B01J20/32 , C07K14/745 , C07K16/00
CPC classification number: C07K14/31 , B01D15/3809 , B01J20/286 , B01J20/3204 , B01J20/3212 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K14/745 , C07K16/00
Abstract: A polypeptide with improved alkaline stability, which polypeptide comprises a mutant of a B or C domain of Staphylococcus Protein A, as specified by SEQ ID NO 1 or SEQ ID NO 2, or of Protein Z, as specified by SEQ ID NO 3, wherein at least the glutamine residue at position 9 has been mutated to an amino acid other than asparagine. The invention also discloses multimers of said polypeptide, as well as separation matrices comprising the multimers or polypeptides.
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公开(公告)号：US20180036445A1
公开(公告)日：2018-02-08
申请号：US15554075
申请日：2016-02-25
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Elin Marianne Monie , Tomas Bjorkman , Anna Gronberg , Anders Ljunglof , Gustav Jose Rodrigo , Karin Torstenson , Magnus Carl Erik Wetterhall
CPC classification number: A61L2/18 , B01D15/203 , B01D15/3804 , G01N30/50
Abstract: The invention discloses a method for cleaning or sanitization of an affinity chromatography matrix, comprising the steps of: a) providing an affinity chromatography matrix having oxidation-tolerant proteinaceous ligands coupled to a support, b) contacting the matrix with a sanitization solution comprising at least one oxidant defined by formula I, R—O—O—H (I) wherein R is hydrogen or an acyl group R′—C(O)—, with R′ being a hydrogen or a methyl, ethyl or propyl group.
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公开(公告)号：US20170327545A1
公开(公告)日：2017-11-16
申请号：US15526849
申请日：2015-12-11
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav Rodrigo , Mats Ander , Tomas Bjorkman
IPC: C07K14/195 , C07K1/22 , C07K16/00
CPC classification number: C07K14/195 , C07K1/22 , C07K16/00 , C07K2317/55 , C07K2317/569 , C07K2319/02
Abstract: A kappa light chain-binding polypeptide comprising or consisting essentially of one or more binding domains of Peptostreptococcus Protein L, each of said domains being selected from the group consisting of domain 2, domain 3 and domain 4.
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公开(公告)号：US20200299326A1
公开(公告)日：2020-09-24
申请号：US16893574
申请日：2020-06-05
Applicant: GE HEALTHCARE BIOPROCESS R&D AB
Inventor： Gustav José Rodrigo , Tomas Bjorkman , Mats Ander , Jesper Ulf Hansson
IPC: C07K1/22 , C07K16/00 , C07K14/31 , B01J20/28 , B01J20/24 , B01D15/38 , C07K17/10 , B01J20/286 , B01J20/32 , C07K16/06
Abstract: The invention relates to a separation matrix comprising at least 11 mg/ml Fc-binding ligands covalently coupled to a porous support, wherein: a) the ligands comprise multimers of alkali-stabilized Protein A domains, and b) the porous support comprises cross-linked polymer particles having a volume-weighted median diameter (d50,v) of 56-70 micrometers and a dry solids weight of 55-80 mg/ml.
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