公开(公告)号：US20180328910A1

公开(公告)日：2018-11-15

申请号：US16046931

申请日：2018-07-26

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  Santiago Fernandez-Gomez

IPC: G01N33/487 ,  C12Q1/6869 ,  B82Y15/00

Abstract: A method of exporting measurements of a nanopore sensor on a nanopore based sequencing chip is disclosed. An electrical characteristic associated with the nanopore sensor is measured. The electrical characteristic associated with the nanopore sensor is processed. A summary for the electrical characteristic and one or more previous electrical characteristics is determined. The summary for the electrical characteristic and the one or more previous electrical characteristics are exported. Determining the summary includes determining that the electrical characteristic and at least a portion of the one or more previous electrical characteristics correspond to a base call event at the nanopore sensor. The summary represents the electrical characteristic and the at least a portion of the one or more previous electrical characteristics.

公开(公告)号：US20170322195A1

公开(公告)日：2017-11-09

申请号：US15488432

申请日：2017-04-14

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Randy Davis

IPC: G01N33/487 ,  B81B1/00 ,  G01N15/12 ,  B82Y5/00

CPC classification number: G01N33/48721 ,  B81B1/00 ,  B82Y5/00 ,  C12Q1/6869 ,  G01N15/12 ,  G01N15/1218

Abstract: A method of forming a nanopore in a lipid bilayer is disclosed. A nanopore forming solution is deposited over a lipid bilayer. The nanopore forming solution has a concentration level and a corresponding activity level of pore molecules such that nanopores are substantially not formed un-stimulated in the lipid bilayer. Formation of a nanopore in the lipid bilayer is initiated by applying an agitation stimulus level to the lipid bilayer. In some embodiments, the concentration level and the corresponding activity level of pore molecules are at levels such that less than 30 percent of a plurality of available lipid bilayers have nanopores formed un-stimulated therein.

公开(公告)号：US20170203520A1

公开(公告)日：2017-07-20

申请号：US15411780

申请日：2017-01-20

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Randy Davis

IPC: B29C67/20 ,  G01N33/487 ,  C12Q1/68

CPC classification number: B29C67/20 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y40/00 ,  C12Q1/6869 ,  G01N33/48721 ,  Y10S977/797 ,  Y10S977/90 ,  Y10S977/942 ,  C12Q2565/631

Abstract: Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.

公开(公告)号：US20170159117A1

公开(公告)日：2017-06-08

申请号：US15435206

申请日：2017-02-16

Applicant: Genia Technologies, Inc.

Inventor： Randall W Davis ,  Roger J.A. Chen

IPC: C12Q1/68 ,  G01N33/487 ,  G01N27/447 ,  B82Y15/00

CPC classification number: C12Q1/6869 ,  B82Y10/00 ,  B82Y15/00 ,  C12Q1/6816 ,  C12Q1/6825 ,  G01N27/447 ,  G01N33/48721 ,  Y10S977/924 ,  C12Q2525/119 ,  C12Q2525/121 ,  C12Q2525/204 ,  C12Q2527/101 ,  C12Q2563/149 ,  C12Q2565/631

Abstract: The present invention relates to a method of using nanopores to obtain sequence information of sample DNAs in ss test DNAs. The method comprises using speed bumps to stall the ss test DNAs in the nanopores at random positions of the ss test DNAs to obtain sequence information of each and every nucleotides of the sample DNAs, and to construct the whole sequences of the sample DNAs. The present invention also relates to identification and/or isolation of test DNAs having desired sequence(s) using nanopore detectors facilitated by speed bump.

公开(公告)号：US20160001250A1

公开(公告)日：2016-01-07

申请号：US14794524

申请日：2015-07-08

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: B01J19/00

CPC classification number: B01J19/0046 ,  B01J2219/00495 ,  B01J2219/00698 ,  G05D23/1919 ,  G05D23/2034 ,  G05D23/2037

Abstract: A system for regulating a temperature of a measurement array is disclosed. The system includes a measurement array including a plurality of sensors, wherein the plurality of sensors are integrated onto an integrated circuit die. The system includes a thermal sensor integrated onto the integrated circuit die, wherein the thermal sensor senses a temperature associated with the plurality of sensors. The system further includes a heat pump coupled to the integrated circuit die, wherein the heat pump is controlled by a feedback control circuit including the thermal sensor.

Abstract translation: 公开了一种用于调节测量阵列的温度的系统。 该系统包括包括多个传感器的测量阵列，其中多个传感器集成在集成电路管芯上。 该系统包括集成在集成电路管芯上的热传感器，其中热传感器感测与多个传感器相关联的温度。 该系统还包括耦合到集成电路管芯的热泵，其中热泵由包括热传感器的反馈控制电路控制。

公开(公告)号：US20140374252A1

公开(公告)日：2014-12-25

申请号：US14335616

申请日：2014-07-18

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Randy Davis

IPC: C12Q1/68 ,  G01N33/487

CPC classification number: B29C67/20 ,  B82Y5/00 ,  B82Y10/00 ,  B82Y40/00 ,  C12Q1/6869 ,  G01N33/48721 ,  Y10S977/797 ,  Y10S977/90 ,  Y10S977/942 ,  C12Q2565/631

Abstract: Techniques for forming a nanopore in a lipid bilayer are described herein. In one example, an agitation stimulus level such as an electrical agitation stimulus is applied to a lipid bilayer wherein the agitation stimulus level tends to facilitate the formation of nanopores in the lipid bilayer. In some embodiments, a change in an electrical property of the lipid bilayer resulting from the formation of the nanopore in the lipid bilayer is detected, and a nanopore has formed in the lipid bilayer is determined based on the detected change in the lipid bilayer electrical property.

Abstract translation: 本文描述了在脂质双层中形成纳米孔的技术。 在一个实例中，将搅动刺激水平（例如电刺激刺激）施加到脂质双层，其中搅动刺激水平倾向于促进脂质双层中的纳米孔的形成。 在一些实施方案中，检测由在脂质双层中形成纳米孔而导致的脂质双层的电性质的变化，并且基于检测到的脂质双层电性能的变化来确定在脂质双层中形成的纳米孔 。

公开(公告)号：US20190212296A1

公开(公告)日：2019-07-11

申请号：US16238451

申请日：2019-01-02

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen ,  Hui Tian ,  J. William Maney, JR.

IPC: G01N27/447 ,  C12Q1/6869 ,  B01L3/00 ,  G01N33/487

CPC classification number: G01N27/44791 ,  B01L3/50273 ,  B01L2400/0421 ,  C12Q1/6869 ,  G01N27/44743 ,  G01N33/48721 ,  C12Q2565/607 ,  C12Q2565/631

Abstract: A method of analyzing a molecule in a nanopore is disclosed. A voltage is applied across a nanopore that is inserted in a membrane by coupling the nanopore to a voltage source. The nanopore is decoupled from the voltage source. After the decoupling, a rate of decay of the voltage across the nanopore is determined. A molecule in the nanopore is distinguished from other possible molecules based on the determined rate of decay of the voltage across the nanopore.

公开(公告)号：US20190079049A1

公开(公告)日：2019-03-14

申请号：US16157019

申请日：2018-10-10

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: G01N27/447 ,  G01N33/487 ,  G01N27/327

CPC classification number: G01N27/44791 ,  G01N27/3278 ,  G01N27/4473 ,  G01N27/44743 ,  G01N33/48721

Abstract: A method of identifying a molecule is disclosed. A molecule is drawn to a nanopore by applying a first voltage signal to a pair of electrodes during a first period, wherein the first voltage signal causes a first ionic current through the nanopore that is indicative of a property of a portion of the molecule proximate to the nanopore. The molecule is released from the nanopore by applying a second voltage signal to the pair of electrodes during a second period, wherein the second voltage signal causes a second ionic current through the nanopore. The first period and the second period are determined based at least in part on a net ionic current through the nanopore comprising the first ionic current and the second ionic current.

公开(公告)号：US20170199151A1

公开(公告)日：2017-07-13

申请号：US15380721

申请日：2016-12-15

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: G01N27/447 ,  C12Q1/68 ,  G01N33/487

CPC classification number: G01N27/3278 ,  C12Q1/6874 ,  G01N27/44791 ,  G01N33/48721

Abstract: A system for communicating information from an array of sensors is disclosed. The system comprises a sensor array that includes a plurality of sensors, wherein each sensor senses a physical property of a material that is in communication with the sensor. The system further comprises signal processing circuitry associated with each sensor that integrates the output of the sensor over time and compares the integrated output to a threshold. The system further comprises a communication network coupled to the signal processing circuitry that outputs information indicating that the integrated output corresponding to a given sensor has reached the threshold.

公开(公告)号：US20170153205A1

公开(公告)日：2017-06-01

申请号：US15288107

申请日：2016-10-07

Applicant: Genia Technologies, Inc.

Inventor： Roger J.A. Chen

IPC: G01N27/447 ,  G01N33/487

CPC classification number: G01N27/44791 ,  G01N27/3278 ,  G01N27/4473 ,  G01N27/44743 ,  G01N33/48721

Abstract: A method of identifying a molecule is disclosed. A molecule is drawn to a nanopore by applying a first voltage signal to a pair of electrodes during a first period, wherein the first voltage signal causes a first ionic current through the nanopore that is indicative of a property of a portion of the molecule proximate to the nanopore. The molecule is released from the nanopore by applying a second voltage signal to the pair of electrodes during a second period, wherein the second voltage signal causes a second ionic current through the nanopore. The first period and the second period are determined based at least in part on a net ionic current through the nanopore comprising the first ionic current and the second ionic current.