公开(公告)号：US20180179558A1

公开(公告)日：2018-06-28

申请号：US15726978

申请日：2017-10-06

Applicant: Braskem S.A.

Inventor： Daniel Johannes Koch ,  Mateus Schreiner LOPES ,  Ane Fernanda Beraldi Zeidler ,  Lucas Pedersen Parizzi

IPC: C12P7/28 ,  C12N9/10 ,  C12N9/02 ,  C12N9/88 ,  C12N9/12 ,  C12N9/90 ,  C12N15/52 ,  C12P5/02 ,  C12P7/04 ,  C12P7/26 ,  C12P7/18

CPC classification number: C12P7/28 ,  C12N9/0008 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/13 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P5/026 ,  C12P7/04 ,  C12P7/18 ,  C12P7/26 ,  C12P2203/00 ,  C12Y102/01021 ,  C12Y203/01009 ,  C12Y207/01047 ,  C12Y208/03008 ,  C12Y401/01004 ,  C12Y401/02013 ,  C12Y501/03

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and one or more three-carbon compounds. Also provided are methods of producing MEG and one or more three-carbon compounds using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and one or more three-carbon compounds.

公开(公告)号：US20160032325A1

公开(公告)日：2016-02-04

申请号：US14885311

申请日：2015-10-16

Applicant: Braskem S.A.

Inventor： Mateus Schreiner Garcez Lopes ,  Avram Michael Slovic ,  Iuri Estrada Gouvea ,  Johana Rincones Perez ,  Lucas Pedersen Parizzi

IPC: C12P5/02 ,  C12N9/88

CPC classification number: C12P5/026 ,  C12N9/88 ,  C12N15/52 ,  C12P5/02 ,  C12Y402/01127

Abstract: The present disclosure generally relates to microorganisms that comprise one or more polynucleotides coding for enzymes in one or more pathways that catalyze a conversion of a fermentable carbon source to butadiene. Also provided are methods of using the microorganisms in industrial processes including, for use in the production of butadiene and products derived therefrom.

公开(公告)号：US11746361B2

公开(公告)日：2023-09-05

申请号：US16841312

申请日：2020-04-06

Applicant: Braskem S.A.

Inventor： Veronica Maria Rodege Gogola Kolling ,  Ane Fernanda Beraldi Zeidler ,  Lucas Pedersen Parizzi

IPC: C12P7/18 ,  C12N1/20 ,  C12P7/42 ,  C12N9/88 ,  C12N9/10 ,  C12N9/12 ,  C12N9/04 ,  C12N9/90 ,  C12P7/28 ,  C12N9/92 ,  C12P7/04 ,  C12N1/21

CPC classification number: C12P7/18 ,  C12N1/20 ,  C12N9/0006 ,  C12N9/1029 ,  C12N9/1205 ,  C12N9/13 ,  C12N9/88 ,  C12N9/90 ,  C12N9/92 ,  C12P7/04 ,  C12P7/28 ,  C12P7/42 ,  C12P2203/00 ,  C12Y503/01005 ,  Y02E50/10

Abstract: The present disclosure provides methods for genetically modifying microbes to produce a microbe capable of simultaneous consumption of xylose and glucose to increase the productivity output of desired chemical products. The disclosure further provides modified bacteria that are capable of simultaneous consumption of xylose and glucose, and compositions comprising the microbes.

公开(公告)号：US10941424B2

公开(公告)日：2021-03-09

申请号：US15726978

申请日：2017-10-06

Applicant: Braskem S.A.

Inventor： Daniel Johannes Koch ,  Mateus Schreiner Lopes ,  Ane Fernanda Beraldi Zeidler ,  Lucas Pedersen Parizzi

IPC: C12N9/10 ,  C12N1/20 ,  C12P7/18 ,  C12P7/28 ,  C12N9/02 ,  C12N9/12 ,  C12N9/88 ,  C12N9/90 ,  C12N15/52 ,  C12P5/02 ,  C12P7/04 ,  C12P7/26

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and one or more three-carbon compounds. Also provided are methods of producing MEG and one or more three-carbon compounds using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and one or more three-carbon compounds.

公开(公告)号：US20180023101A1

公开(公告)日：2018-01-25

申请号：US15726983

申请日：2017-10-06

Applicant: Braskem S.A.

Inventor： Daniel Johannes KOCH ,  Mateus Schreiner Lopes ,  Ane Fernanda Beraldi Zeidler ,  Lucas Pedersen Parizzi

IPC: C12P7/28 ,  C12N9/02 ,  C12N9/88 ,  C12N9/12 ,  C12P7/18 ,  C12N15/52 ,  C12P5/02 ,  C12P7/04 ,  C12P7/26 ,  C12N9/10 ,  C12N9/90

Abstract: The present application relates to recombinant microorganisms useful in the biosynthesis of monoethylene glycol (MEG) and one or more three-carbon compounds such as acetone, isopropanol or propene. The MEG and one or more three-carbon compounds described herein are useful as starting material for production of other compounds or as end products for industrial and household use. The application further relates to recombinant microorganisms co-expressing a C2 branch pathway and a C3 branch pathway for the production of MEG and one or more three-carbon compounds. Also provided are methods of producing MEG and one or more three-carbon compounds using the recombinant microorganisms, as well as compositions comprising the recombinant microorganisms and/or optionally the products MEG and one or more three-carbon compounds.

公开(公告)号：US20150064760A1

公开(公告)日：2015-03-05

申请号：US14479071

申请日：2014-09-05

Applicant: BRASKEM S.A.

Inventor： Johana Rincones Perez ,  Juan Diego Rojas Rojas ,  Ane Fernanda Beraldi Zeidler ,  Aline Silva Romao Dumaresq ,  Marilene Elizabete Pavan Rodrigues ,  Iuri Estrada Gouvea ,  Felipe Galzerani ,  Daniel Johannes Koch ,  Lucas Pedersen Parizzi ,  Mateus Schreiner Garcez Lopes ,  Thomas Martin Halder ,  Antonio Luis Ribeiro De Castro Morschbacker ,  Avram Michael Slovic

IPC: C12P5/02 ,  C12P7/04 ,  C12P7/18 ,  C12N15/63

CPC classification number: C12P7/18 ,  C07K14/395 ,  C12N15/63 ,  C12P5/026 ,  C12P7/04

Abstract: The present disclosure provides a non-naturally occurring microorganism comprising: one or more polynucleotides encoding one or more enzymes in a pathway that produces acetyl-CoA; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of crotonyl alcohol, 5-hydroxy-3-ketovaleryl-CoA, 3-ketopent-4-enoyl-CoA, or 3,5-ketovaleryl-CoA to butadiene; one or more polynucleotides encoding one or more enzymes in a pathway that catalyze a conversion of dihydroxyacetone-phosphate to 1-propanol and/or 1,2-propanediol, wherein the microorganism has reduced levels of pyruvate decarboxylase enzymatic activity (e.g., the microorganism comprises a disruption of one or more enzymes that decarboxylate pyruvate and/or a disruption of one or more transcription factors of one or more enzymes that decarboxylate pyruvate), and wherein the microorganism is capable of growing on a C6 sugar as a sole carbon source under anaerobic conditions. Also provided are methods of using the disclosed non-naturally occurring microorganisms in methods for the coproduction of butadiene and 1-propanol and/or 1,2-propanediol.

Abstract translation: 本公开提供了非天然存在的微生物，其包含：在产生乙酰辅酶A的途径中编码一种或多种酶的一种或多种多核苷酸; 在催化巴豆醇，5-羟基-3-酮戊酰基-CoA，3-酮戊-4-烯酰基-CoA或3,5-酮戊酰基-CoA转化为丁二烯的途径中编码一种或多种酶的一种或多种多核苷酸 ; 一种或多种编码一种或多种酶的多核苷酸，其在催化二羟丙基磷酸盐转化为1-丙醇和/或1,2-丙二醇的途径中，其中微生物具有降低的丙酮酸脱羧酶水平的酶活性（例如，微生物包含 破坏一种或多种脱羧酸丙酮酸酶和/或破坏一种或多种丙酮酸脱羧酸酶的一种或多种转录因子的酶），并且其中所述微生物能够生长在C6糖作为唯一碳源的厌氧 条件。 还提供了在共同生产丁二烯和1-丙醇和/或1,2-丙二醇的方法中使用所公开的非天然存在的微生物的方法。