公开(公告)号：US20240101945A1

公开(公告)日：2024-03-28

申请号：US18374435

申请日：2023-09-28

Applicant: Genzyme Corporation

Inventor： Kevin Brower ,  Michael Coolbaugh ,  Xhorxhi Gjoka ,  Chad Varner ,  Jason Walther

IPC: C12M3/00 ,  B01D15/18 ,  B01D15/38 ,  C12M1/00

CPC classification number: C12M23/44 ,  B01D15/1821 ,  B01D15/3804 ,  C12M47/10 ,  C12M47/12

Abstract: Systems and devices for producing biological products and methods of using the same are provided. An example universal skid includes a rigid support and a plurality of flow-channel hardware sets. Each flow-channel hardware set couples with a respective flow channel of a plurality of flow channels for unit operations. The flow-channel hardware sets allow the flow channels to operate in parallel or in series for continuous processing over the unit operations. An example modular assembly includes one or more single-use kits for one or more unit operations and a universal skid coupled with the one or more single-use kits. An example manufacturing system includes multiple modular assemblies coupled with each other allowing continuous processing within each modular assembly, in between the modular assemblies, fully continuous processing across multiple unit operations.

公开(公告)号：US20230340396A1

公开(公告)日：2023-10-26

申请号：US18308870

申请日：2023-04-28

Applicant: Genzyme Corporation

Inventor： Jiuyi Lu ,  Jason Walther ,  Jonathan Wang ,  Kevin Victor Chen

IPC: C12M1/36 ,  B01D61/18 ,  B01D15/18 ,  B01D61/22 ,  C12M1/00 ,  C12M1/34

CPC classification number: C12M41/48 ,  B01D15/1807 ,  B01D61/18 ,  B01D61/22 ,  C12M29/04 ,  C12M29/10 ,  C12M41/40 ,  B01D2311/2626 ,  B01D2311/2688 ,  B01D2313/243 ,  B01D2315/10 ,  B01D2317/04 ,  B01D2317/06

Abstract: The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.

公开(公告)号：US20190338238A1

公开(公告)日：2019-11-07

申请号：US16403161

申请日：2019-05-03

Applicant: Genzyme Corporation

Inventor： Jiuyi Lu ,  Jason Walther ,  Jonathan Wang ,  Kevin Victor Chen

IPC: C12M1/36 ,  B01D61/18 ,  B01D61/22 ,  B01D15/18 ,  C12M1/00 ,  C12M1/34

Abstract: The disclosure provides a filtration system for a cell culture apparatus and a method of cell culture. The filtration system comprises a bioreactor vessel and two or more alternating tangential flow (ATF) filters connected in parallel. A failure in either filter is detected by an in-line sensor, and an automated response system functions to sequester the malfunctioning filter by stopping the flow of liquid media through the filter. Media flow through the remaining operable filters can be increased so that the rate of perfusion through the bioreactor remains relatively unchanged. Such a system may prevent issues that arise from ATF filter failures in conventional perfusion bioreactors, thereby improving the long-term viability of cell cultures.

公开(公告)号：US20160177361A1

公开(公告)日：2016-06-23

申请号：US14976486

申请日：2015-12-21

Applicant: Genzyme Corporation

Inventor： Christopher Hwang ,  Timothy Johnson ,  Jason Walther ,  Cheng Cheng ,  Jonathan Wang ,  Neha Shah ,  Seul-A Bae

IPC: C12P21/00 ,  C07K16/00

CPC classification number: C12P21/00 ,  C07K16/00 ,  C07K2317/14 ,  C12N5/0043 ,  C12N5/005 ,  C12N2500/50 ,  C12N2510/02

Abstract: Provided herein are methods of culturing a mammalian cell in a liquid medium including poloxamer-188 at a concentration of 1.8 g/L or at a greater concentration than 1.8 g/L more or a liquid medium that includes a poloxamer-188 concentration that is selected based on one or more factors selected from the group of: pore size, pore type, gas flow rate, viable cell density in the medium, and markers related to cell stress.

Abstract translation: 本文提供了在液体培养基中培养哺乳动物细胞的方法，所述液体培养基包括浓度为1.8g / L或浓度高于1.8g / L的泊洛沙姆-188或包含选择的泊洛沙姆188浓度的液体培养基 基于选自以下组中的一种或多种因素：孔径，孔型，气体流速，培养基中的活细胞密度和与细胞应激相关的标记。