-
公开(公告)号：US5888780A
公开(公告)日：1999-03-30
申请号：US802233
申请日：1997-02-19
IPC分类号： C07H21/00 , C07K14/47 , C07K14/82 , C12N9/12 , C12N15/10 , C12N15/113 , C12Q1/68 , C12Q1/70 , C12P19/34
CPC分类号： C12N15/113 , C07H21/00 , C07K14/4746 , C07K14/82 , C12N15/10 , C12N9/1252 , C12Q1/682 , C12Q1/6823 , C12Q1/686 , C12Q1/6886 , Y10S435/975
摘要： The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5' nucleases and 3' exonucleases, are used to screen for known and unknown mutations, including single base changes, in nucleic acids. Methods are provided which allow for the identification of genetic mutations and the identification bacterial and viral strains and species in a sample.
摘要翻译： 本发明涉及以位点特异性方式切割核酸切割结构的方法。 包括5'核酸酶和3'核酸外切酶在内的酶用于筛选核酸中已知和未知突变，包括单碱基变化。 提供了允许鉴定基因突变和鉴定样品中的细菌和病毒菌株和物种的方法。
-
公开(公告)号：US5614402A
公开(公告)日：1997-03-25
申请号：US254359
申请日：1994-06-06
IPC分类号： C07H21/00 , C07K14/47 , C12N9/12 , C12N15/09 , C12N15/10 , C12N15/113 , C12Q1/68 , C12Q1/70 , G01N33/53 , C12N9/22
CPC分类号： C12N15/113 , C07H21/00 , C07K14/4746 , C12N15/10 , C12N9/1252 , C12N9/14 , C12Q1/682 , C12Q1/6823 , C12Q1/6825 , C12Q1/6827 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , C12Q1/705 , C12Q2600/156
摘要： A means cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.
摘要翻译： 公开了以站点特异性方式切割核酸切割结构的方法。 采用具有5'核酸酶活性而不干扰核酸合成能力的切割酶作为检测特异性核酸序列的新方法的基础。
-
公开(公告)号：US08026050B1
公开(公告)日：2011-09-27
申请号：US09660924
申请日：2000-09-13
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , C07H21/00 , C07K14/4746 , C07K14/82 , C12N9/1252 , C12N15/10 , C12N15/113 , C12Q1/682 , C12Q1/6823 , C12Q1/683 , C12Q1/689 , C12Q2537/149 , C12Q2525/301 , C12Q2521/319
摘要： The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
摘要翻译： 本发明涉及以位点特异性方式切割核酸切割结构的方法。 酶包括5'核酸酶和3'核酸外切酶，用于检测和鉴定衍生自微生物的核酸。 提供了允许在样品中检测和鉴定细菌和病毒病原体的方法。
-
公开(公告)号：US07588891B2
公开(公告)日：2009-09-15
申请号：US11183077
申请日：2005-07-15
IPC分类号： C12Q1/68
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301 , C12Q2563/149
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
-
公开(公告)号：US20080268455A1
公开(公告)日：2008-10-30
申请号：US12099656
申请日：2008-04-08
IPC分类号： C12Q1/68
CPC分类号： C12N9/22 , C12Q1/6823 , C12Q1/6827 , Y10S435/81 , Y10S435/822 , C12Q2565/525 , C12Q2561/109 , C12Q2525/301
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。 本发明还涉及基于电荷分离核酸分子的方法和装置。 本发明还提供了通过形成完整和活化的蛋白质结合区域来检测非靶切割产物的方法。 本发明还提供用于检测样品中巨细胞病毒核酸的敏感和具体方法。
-
公开(公告)号：US07273696B2
公开(公告)日：2007-09-25
申请号：US09941095
申请日：2001-08-28
IPC分类号： C12Q1/68
CPC分类号： C12N15/113 , C07H21/00 , C07K14/4746 , C07K14/82 , C12N9/1252 , C12N15/10 , C12Q1/68 , C12Q1/682 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , C12Q1/6853 , C12Q1/686 , C12Q1/689 , C12Q1/705 , Y10T436/143333 , C12Q2537/149 , C12Q2525/301 , C12Q2521/319
摘要： The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5′ nucleases and 3′ exonucleases, are used to detect and identify nucleic acids derived from microorganisms. Methods are provided which allow for the detection and identification of bacterial and viral pathogens in a sample.
摘要翻译： 本发明涉及以位点特异性方式切割核酸切割结构的方法。 酶包括5'核酸酶和3'核酸外切酶，用于检测和鉴定衍生自微生物的核酸。 提供了允许在样品中检测和鉴定细菌和病毒病原体的方法。
-
17.发明授权Polymorphism analysis by nucleic acid structure probing with structure-bridging oligonucleotides 失效通过结构桥接寡核苷酸的核酸结构探测进行多态性分析公开(公告)号：US06709819B2
公开(公告)日：2004-03-23
申请号：US09825574
申请日：2001-04-03
申请人： Victor I. Lyamichev , Fang Dong , Mary Ann D. Brow , Lance Fors , Bruce P. Neri
发明人： Victor I. Lyamichev , Fang Dong , Mary Ann D. Brow , Lance Fors , Bruce P. Neri
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q2565/133
摘要： The present invention relates to methods and compositions for analyzing nucleic acids. In particular, the present invention provides methods and compositions for the detection and characterization of nucleic acid sequences and sequence changes. The methods of the present invention permit the detection and/or identification of genetic polymorphism such as those associated with human disease and permit the identification of pathogens (e.g., viral and bacterial strain identification).
摘要翻译： 本发明涉及用于分析核酸的方法和组合物。 特别地，本发明提供了用于检测和表征核酸序列和序列变化的方法和组合物。 本发明的方法允许检测和/或鉴定遗传多态性，例如与人类疾病相关的遗传多态性，并允许鉴定病原体（例如病毒和细菌菌株鉴定）。
-
公开(公告)号：US06709815B1
公开(公告)日：2004-03-23
申请号：US09402618
申请日：2000-07-18
申请人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
发明人： Fang Dong , Victor I. Lyamichev , James R. Prudent , Lance Fors , Bruce P. Neri , Mary Ann D. Brow , Todd A. Anderson , James E. Dahlberg
IPC分类号： C12Q168
CPC分类号： C07H21/04 , C07H21/02 , C12Q1/68 , C12Q1/6827 , Y02A90/26 , C12Q2565/131 , C12Q2565/501 , C12Q2565/625 , C12Q2565/133
摘要： The present invention relates to methods and compns. for treating nucleic acids, and in particular, methods and compns. for the detection and characterization of nucleic acid sequences and sequence changes. The invention provides methods for examg. the conformations assumed by single strands of nucleic acid, forming the basis of novel methods of detection of specific nucleic acid sequences. The present invention contemplates use of novel detection methods for, among other uses, clinical diagnostic purposes, including but not limited to the detection and identification of pathogenic organisms. Examples are presented for the analysis of Mycobacterium tuberculosis and hepatitis C virus genes.
摘要翻译： 本发明涉及方法和方法。 用于处理核酸，特别是方法和化合物。 用于检测和表征核酸序列和序列变化。 本发明提供了检查方法。 由单链核酸假设的构象，形成检测特异性核酸序列的新方法的基础。 本发明考虑使用新的检测方法用于临床诊断目的，包括但不限于病原体的检测和鉴定。 介绍了分析结核分枝杆菌和丙型肝炎病毒基因的实例。
-
公开(公告)号：US5994069A
公开(公告)日：1999-11-30
申请号：US823516
申请日：1997-03-24
IPC分类号： C12N15/09 , C12N1/21 , C12N9/12 , C12N9/16 , C12N9/22 , C12Q1/68 , C12R1/92 , G01N33/50 , G01N33/566 , C12P19/34
CPC分类号： C12N9/22 , C12N9/1252 , C12Q1/6816 , C12Q1/6823 , C12Q1/6827 , C12Q1/683 , C12Q1/70 , C12Q1/701
摘要： The present invention relates to means for the detection and characterization of nucleic acid sequences, as well as variations in nucleic acid sequences. The present invention also relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. The structure-specific nuclease activity of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof. The present invention further relates to methods and devices for the separation of nucleic acid molecules based on charge. The present invention also provides methods for the detection of non-target cleavage products via the formation of a complete and activated protein binding region. The invention further provides sensitive and specific methods for the detection of human cytomegalovirus nucleic acid in a sample.
摘要翻译： 本发明涉及核酸序列的检测和表征以及核酸序列的变化的方法。 本发明还涉及在靶序列上形成核酸切割结构并以位点特异性方式切割核酸切割结构的方法。 使用各种酶的结构特异性核酸酶活性来切割靶依赖性切割结构，从而指示特异性核酸序列的存在或其特定变体。 本发明还涉及基于电荷分离核酸分子的方法和装置。 本发明还提供了通过形成完整和活化的蛋白质结合区域来检测非靶切割产物的方法。 本发明还提供用于检测样品中巨细胞病毒核酸的敏感和具体方法。
-
公开(公告)号：US5795763A
公开(公告)日：1998-08-18
申请号：US481238
申请日：1995-06-06
IPC分类号： C12N1/21 , C07H21/00 , C07K14/47 , C12N9/12 , C12N9/14 , C12N9/16 , C12N15/09 , C12N15/10 , C12N15/113 , C12N15/55 , C12Q1/44 , C12Q1/68 , C12Q1/70 , C12R1/19 , G01N33/53 , C12N9/22
CPC分类号： C12N9/14 , C07H21/00 , C07K14/4746 , C12N15/10 , C12N15/113 , C12N9/1252 , C12Q1/682 , C12Q1/6823 , C12Q1/6827 , C12Q1/6844 , C12Q1/6853 , C12Q1/686 , C12Q1/705 , C12Q2600/156 , Y10T436/143333
摘要： A means for cleaving a nucleic acid cleavage structure in a site-specific manner is disclosed. A cleaving enzyme having 5' nuclease activity without interfering nucleic acid synthetic ability is employed as the basis of a novel method of detection of specific nucleic acid sequences.
摘要翻译： 公开了以位点特异性方式切割核酸切割结构的方法。 采用具有5'核酸酶活性而不干扰核酸合成能力的切割酶作为检测特异性核酸序列的新方法的基础。
-
-
-
-
-
-
-
-
-
搜索结果
59 条记录 (耗时 0.113 秒)
