公开(公告)号：US08566038B2

公开(公告)日：2013-10-22

申请号：US11582938

申请日：2006-10-17

Applicant: James K. Gimzewski ,  Bhubaneswar Mishra ,  Jason C. Reed

Inventor： James K. Gimzewski ,  Bhubaneswar Mishra ,  Jason C. Reed

IPC: G01Q10/00 ,  G01N33/48

CPC classification number: C12Q1/6834 ,  C12Q1/683 ,  C12Q2565/601 ,  C12Q2565/518 ,  C12Q2521/331 ,  C12Q2522/101

Abstract: The present invention provides methods of detecting a nucleic acid analyte in a sample. The present invention further provides a method for assigning a profile of a feature to a nucleic acid. The present invention further provides a computer program product for use in a subject method. The present invention further provides a system for detecting a nucleic acid in a sample; and a system for assigning a profile of a feature to a nucleic acid. The present invention further provides a method for immobilizing a nucleic acid onto an insoluble support; and further provides insoluble support having nucleic acid(s) immobilized thereon. The present invention further provides a method of diagnosing a disorder or condition in an individual, where the method involves use of a subject method for detecting a nucleic acid analyte.

Abstract translation: 本发明提供了检测样品中核酸分析物的方法。 本发明还提供了将特征的轮廓分配给核酸的方法。 本发明还提供一种用于本发明方法的计算机程序产品。 本发明还提供了一种用于检测样品中核酸的系统; 以及用于将特征的轮廓分配给核酸的系统。 本发明还提供了将核酸固定在不溶性载体上的方法; 并进一步提供其上固定有核酸的不溶性载体。 本发明还提供了诊断个体中的病症或病症的方法，其中所述方法涉及使用本发明的方法来检测核酸分析物。

公开(公告)号：US20100284016A1

公开(公告)日：2010-11-11

申请号：US12436702

申请日：2009-05-06

Applicant: Michael A. Teitell ,  James K. Gimzewski ,  Jason C. Reed

Inventor： Michael A. Teitell ,  James K. Gimzewski ,  Jason C. Reed

IPC: G01J3/45 ,  G02B21/24 ,  G02B21/36 ,  G01B9/021 ,  G02B3/10

CPC classification number: G01N33/5008 ,  G01J3/453 ,  G02B5/126 ,  G02B21/0056 ,  G02B21/32

Abstract: The present invention provides optical systems and methods for determining a characteristic of a cell, such as cell type, cellular response to a biochemical event, biological state and the like. The methods typically involve using interferometry to observe membrane properties in a cell and then use this information to determine one or more characteristics of a cell. The methods of the invention are useful for applications such as drug screening as well as diagnostic techniques.

Abstract translation: 本发明提供了用于确定细胞特征的光学系统和方法，例如细胞类型，对生物化学事件的细胞反应，生物学状态等。 所述方法通常涉及使用干涉测量来观察细胞中的膜特性，然后使用该信息来确定细胞的一个或多个特征。 本发明的方法对于诸如药物筛选以及诊断技术的应用是有用的。

公开(公告)号：US4698502A

公开(公告)日：1987-10-06

申请号：US821946

申请日：1986-01-24

Applicant: Johannes G. Bednorz ,  James K. Gimzewski ,  Bruno Reihl

Inventor： Johannes G. Bednorz ,  James K. Gimzewski ,  Bruno Reihl

IPC: H01J37/28 ,  H01J37/073 ,  H01J37/256 ,  G01N23/00 ,  H01J37/26

CPC classification number: H01J37/256 ,  H01J37/073

Abstract: An Auger electron microscope is equipped with a field-emission tip maintained at an essentially constant distance above the surface of the specimen. The tip may consist of a tungsten (100) whisker having a radius of .about.50 nm at the apex, the working distance being on the order of 1 mm. Auger electrons emitted from the surface of the specimen are collected by an electron energy analyzer for conventional processing. Mutual scanning displacement between the tip and specimen is obtained through use of an xyz-drive module, which is also responsible for adjusting the working distance of the tip. The entire microscope setup is mounted on vibration damping means and may be inserted into a vacuum system by means of an appropriate flange, if desired.

Abstract translation: 俄歇电子显微镜配备有一个场致发射尖端，其保持在样品表面上方基本恒定的距离处。 尖端可以由顶点处的半径为50nm的钨（100）晶须组成，工作距离在1mm左右。 通过电子能量分析仪收集从样品表面发射的俄歇电子，用于常规处理。 通过使用xyz驱动模块获得尖端和样品之间的相互扫描位移，该模块也负责调整尖端的工作距离。 整个显微镜装置安装在减震装置上，如果需要，可以通过适当的凸缘插入真空系统中。

公开(公告)号：US08652798B2

公开(公告)日：2014-02-18

申请号：US12993826

申请日：2008-12-01

Applicant: James K. Gimzewski ,  Sarah E. Cross ,  Yusheng Jin ,  Jianyu Rao

Inventor： James K. Gimzewski ,  Sarah E. Cross ,  Yusheng Jin ,  Jianyu Rao

IPC: C12Q1/02 ,  B82B3/00

CPC classification number: G01Q30/025 ,  B82Y15/00 ,  B82Y35/00 ,  G01Q20/00 ,  G01Q30/14 ,  G01Q60/366 ,  Y10S977/92

Abstract: Described herein is the analysis of nanomechanical characteristics of cells. In particular, changes in certain local nanomechanical characteristics of ex vivo human cells can correlate with presence of a human disease, such as cancer, as well as a particular stage of progression of the disease. Also, for human patients that are administered with a therapeutic agent, changes in local nanomechanical characteristics of ex vivo cells collected from the patients can correlate with effectiveness of the therapeutic agent in terms of impeding or reversing progression of the disease. By exploiting this correlation, systems and related methods can be advantageously implemented for disease state detection and therapeutic agent selection and monitoring.

Abstract translation: 这里描述的是细胞的纳米机械特性的分析。 特别地，离体人细胞的某些局部纳米机械特征的变化可以与人类疾病如癌症的存在以及疾病进展的特定阶段相关联。 此外，对于施用治疗剂的人类患者，从患者收集的离体细胞的局部纳米机械特征的变化可以与治疗剂在阻碍或逆转疾病进展方面的有效性相关。 通过利用这种相关性，系统和相关方法可以有利地实现用于疾病状态检测和治疗剂选择和监测。

公开(公告)号：US08524488B2

公开(公告)日：2013-09-03

申请号：US11077266

申请日：2005-03-09

Applicant: James K. Gimzewski ,  Andrew E. Pelling

Inventor： James K. Gimzewski ,  Andrew E. Pelling

IPC: C12M1/34

CPC classification number: G01N33/5088 ,  G01N33/5008 ,  G01N33/502 ,  G01N33/5076 ,  G01N33/5091 ,  G01Q20/02 ,  G01Q60/24 ,  Y02A90/26

Abstract: The present invention provides methods of determining a characteristic of a cell, such as cell type, cellular response to a biochemical event, and biological state. The method generally involves detecting membrane movement in a cell to determine a characteristic of a cell. The methods of the invention are useful for applications such as drug screening and diagnostics. The invention further provides databases of cell characteristics, as determined by the instant methods. The invention further provides systems for determining the characteristic of a cell.

Abstract translation: 本发明提供了确定细胞特征的方法，例如细胞类型，对生物化学事件的细胞反应和生物学状态。 该方法通常涉及检测细胞中的膜运动以确定细胞的特征。 本发明的方法对于诸如药物筛选和诊断的应用是有用的。 本发明还提供了通过本方法确定的小区特征的数据库。 本发明还提供用于确定电池特性的系统。

公开(公告)号：US08501092B2

公开(公告)日：2013-08-06

申请号：US10589430

申请日：2005-04-14

Applicant: James K. Gimzewski

Inventor： James K. Gimzewski

IPC: G01N33/00

CPC classification number: G01N25/20 ,  G01K1/16 ,  G01K17/00

Abstract: The present invention provides a calorimeter device, generally comprising a reaction vessel which may be U-shaped and which may be cantilevered; and a sensor for detecting temperature changes. In various embodiments, the sensor detects heat input into or output from the reaction vessel; changes in the electrical properties of a material coated onto the reaction vessel; changes in the mechanical properties of the reaction vessel; or changes in the resonance properties of the reaction vessel. The present invention further provides arrays of a subject calorimeter device. The present invention further provides a system for detecting a temperature change. The present invention further provides methods of detecting a temperature change that occurs as a result of a chemical, biochemical, biological, light-induced, or physical process. The methods generally involve introducing a sample into a subject device, and detecting a temperature change.

Abstract translation: 本发明提供了一种热量计装置，其通常包括可以是U形并且可以悬臂的反应容器; 以及用于检测温度变化的传感器。 在各种实施例中，传感器检测进入或从反应容器输出的热量; 涂覆在反应容器上的材料的电性能的变化; 反应容器机械性能的变化; 或反应容器的共振特性的变化。 本发明还提供一种主题量热仪装置的阵列。 本发明还提供一种用于检测温度变化的系统。 本发明还提供了检测作为化学，生物化学，生物学，光诱导或物理过程的结果而发生的温度变化的方法。 所述方法通常涉及将样品引入到本体装置中并检测温度变化。

公开(公告)号：US20110070604A1

公开(公告)日：2011-03-24

申请号：US12993826

申请日：2008-12-01

Applicant: James K. Gimzewski ,  Sarah E. Cross ,  Yusheng Jin ,  Jianyu Rao

Inventor： James K. Gimzewski ,  Sarah E. Cross ,  Yusheng Jin ,  Jianyu Rao

IPC: C12Q1/02 ,  C12M1/34 ,  B82Y15/00 ,  B82Y5/00

CPC classification number: G01Q30/025 ,  B82Y15/00 ,  B82Y35/00 ,  G01Q20/00 ,  G01Q30/14 ,  G01Q60/366 ,  Y10S977/92

Abstract: Described herein is the analysis of nanomechanical characteristics of cells. In particular, changes in certain local nanomechanical characteristics of ex vivo human cells can correlate with presence of a human disease, such as cancer, as well as a particular stage of progression of the disease. Also, for human patients that are administered with a therapeutic agent, changes in local nanomechanical characteristics of ex vivo cells collected from the patients can correlate with effectiveness of the therapeutic agent in terms of impeding or reversing progression of the disease. By exploiting this correlation, systems and related methods can be advantageously implemented for disease state detection and therapeutic agent selection and monitoring.

Abstract translation: 这里描述的是细胞的纳米机械特性的分析。 特别地，离体人细胞的某些局部纳米机械特征的变化可以与人类疾病如癌症的存在以及疾病进展的特定阶段相关联。 此外，对于施用治疗剂的人类患者，从患者收集的离体细胞的局部纳米机械特征的变化可以与治疗剂在阻碍或逆转疾病进展方面的有效性相关。 通过利用这种相关性，系统和相关方法可以有利地实现用于疾病状态检测和治疗剂选择和监测。

公开(公告)号：US07741615B2

公开(公告)日：2010-06-22

申请号：US11596586

申请日：2005-04-22

Applicant: Seth Putterman ,  James K. Gimzewski ,  Brian B. Naranjo

Inventor： Seth Putterman ,  James K. Gimzewski ,  Brian B. Naranjo

IPC: H01J27/00

CPC classification number: H01J27/02 ,  H01J1/30 ,  H01J2201/306

Abstract: Ferroelectric, pyroelectric and piezoelectric crystals are used to generate spatially localized high energy (up to and exceeding 100 keV) electron and ion beams, which may be used in a wide variety of applications including pulsed neutron generation, therapeutic X-ray/electron devices, elemental analysis, local scanning chemical analysis, high energy scanning microscopy, point source compact transmission electron microscopy, compact ion beam sources, positron sources, micro-thrusters for ion engines, and improved fusion efficiency especially of the Farnsworth type. The high-energy emission can be created by simply heating the material or by application of external coercive electromagnetic and acoustic fields.

Abstract translation: 铁电，热电和压电晶体用于产生空间局部的高能（高达和超过100keV）的电子和离子束，其可用于各种应用，包括脉冲中子生成，治疗性X射线/电子器件， 元素分析，局部扫描化学分析，高能扫描显微镜，点源紧凑型透射电子显微镜，紧凑型离子束源，正电子源，离子发动机的微推进器，以及特别是Farnsworth型的融合效率的提高。 可以通过简单地加热材料或通过施加外部矫顽电磁场和声场来产生高能量发射。

公开(公告)号：US20080251735A1

公开(公告)日：2008-10-16

申请号：US11596586

申请日：2005-04-22

Applicant: Seth Putterman ,  James K. Gimzewski ,  Brian B. Naranjo

Inventor： Seth Putterman ,  James K. Gimzewski ,  Brian B. Naranjo

IPC: H01J27/02 ,  H01J29/46

CPC classification number: H01J27/02 ,  H01J1/30 ,  H01J2201/306

Abstract: Ferroelectric, pyroelectric and piezoelectric crystals are used to generate spatially localized high energy (up to and exceeding 100 keV) electron and ion beams, which may be used in a wide variety of applications including pulsed neutron generation, therapeutic X-ray/electron devices, elemental analysis, local scanning chemical analysis, high energy scanning microscopy, point source compact transmission electron microscopy, compact ion beam sources, positron sources, micro-thrusters for ion engines, and improved fusion efficiency especially of the Farnsworth type. The high-energy emission can be created by simply heating the material or by application of external coercive electromagnetic and acoustic fields.

Abstract translation: 铁电，热电和压电晶体用于产生空间局部的高能（高达和超过100keV）的电子和离子束，其可用于各种应用，包括脉冲中子生成，治疗性X射线/电子器件， 元素分析，局部扫描化学分析，高能扫描显微镜，点源紧凑型透射电子显微镜，紧凑型离子束源，正电子源，离子发动机的微推进器，以及特别是Farnsworth型的融合效率的提高。 可以通过简单地加热材料或通过施加外部矫顽电磁场和声场来产生高能量发射。