ACETYL LYSINE INCORPORATION WITH tRNA SYNTHETASE
    12.
    发明申请
    ACETYL LYSINE INCORPORATION WITH tRNA SYNTHETASE 审中-公开
    乙酰胆碱配合tRNA合成酶

    公开(公告)号:US20120190825A1

    公开(公告)日:2012-07-26

    申请号:US13499340

    申请日:2010-10-01

    CPC分类号: C12P21/02 C12N9/93

    摘要: The invention relates to a tRNA synthetase capable of binding Nε-acetyl lysine, wherein said synthetase comprises a polypeptide having at least 90% sequence identity to the amino acid sequence of MbPyIRS, and wherein said synthetase comprises a L266M mutation.

    摘要翻译: 本发明涉及能够结合N-乙酰基赖氨酸的tRNA合成酶,其中所述合成酶包含与MbPyIRS的氨基酸序列具有至少90%序列同一性的多肽,并且其中所述合成酶包含L266M突变。

    Evolved orthogonal ribosomes
    17.
    发明授权
    Evolved orthogonal ribosomes 失效
    进化正交核糖体

    公开(公告)号:US08497231B2

    公开(公告)日:2013-07-30

    申请号:US12516230

    申请日:2007-11-28

    摘要: There is provided a method for evolving an orthogonal rRNA molecule, comprising the steps of: providing one or more libraries of mutant orthogonal rRNA molecules and introducing the libraries into cells such that the orthogonal rRNA is incorporated into ribosomes to provide orthogonal ribosomes; providing one or more orthogonal mRNA molecules which (i) are not translated by natural ribosomes, and (ii) comprise one or more orthogonal mRNA codons; assaying the translation of the orthogonal mRNA and selecting the orthogonal rRNA molecules which translate the orthogonal mRNA, wherein the assay in step (c) requires translation of one or more orthogonal mRNA codons in the orthogonal mRNA; and orthogonal ribosomes incorporating such rRNA molecules.

    摘要翻译: 提供了用于进化正交rRNA分子的方法,包括以下步骤:提供一个或多个突变正交rRNA分子文库,并将文库引入细胞,使得正交rRNA被并入核糖体中以提供正交核糖体; 提供(i)不被天然核糖体翻译的一个或多个正交mRNA分子,和(ii)包含一个或多个正交mRNA密码子; 测定正交mRNA的翻译并选择翻译正交mRNA的正交rRNA分子,其中步骤(c)中的测定需要在正交mRNA中翻译一个或多个正交的mRNA密码子; 并结合这样的rRNA分子的正交核糖体。

    Twin Buckle Assembly with Dual Release Positions
    19.
    发明申请
    Twin Buckle Assembly with Dual Release Positions 有权
    双扣单元双释放位置

    公开(公告)号:US20100186204A1

    公开(公告)日:2010-07-29

    申请号:US12546914

    申请日:2009-08-25

    申请人: Jason Chin Marc Jung

    发明人: Jason Chin Marc Jung

    IPC分类号: A44B11/25

    摘要: Disclosed is a twin buckle assembly with dual release positions. More particularly, the invention relates to a buckle assembly that can lockingly secure two buckle tongues. The buckle assembly includes a single, pivotal latch mechanism that can separately secure one or both of the buckle tongues. Pivotal movement of the latch occurs upon rotation of the buckle cover. Thus, one tongue is released at a first rotational position of the cover and both tongues are released at a second rotational position of the cover. The buckle assembly, thereby, allows the independent removal of lap and shoulder belts.

    摘要翻译: 公开了具有双重释放位置的双扣件。 更具体地,本发明涉及一种能够锁定地固定两个带扣舌的带扣组件。 带扣组件包括单独的枢转闩锁机构,其可以单独地固定一个或两个带扣舌。 闩锁的枢转运动发生在带扣盖旋转时。 因此,一个舌片在盖的第一旋转位置被释放,并且两个舌片在盖子的第二旋转位置被释放。 因此,带扣组件可以独立地拆卸搭扣和肩带。